ATE161881T1 - Verwendung von promotoren von filamentösen pilzen - Google Patents

Verwendung von promotoren von filamentösen pilzen

Info

Publication number
ATE161881T1
ATE161881T1 AT86902449T AT86902449T ATE161881T1 AT E161881 T1 ATE161881 T1 AT E161881T1 AT 86902449 T AT86902449 T AT 86902449T AT 86902449 T AT86902449 T AT 86902449T AT E161881 T1 ATE161881 T1 AT E161881T1
Authority
AT
Austria
Prior art keywords
coding regions
expression
foreign
aspergillus
secretion
Prior art date
Application number
AT86902449T
Other languages
English (en)
Inventor
David Ivor Gwynne
Francis Paul Buxton
Mark Hudson Pickett
Roger Wayne Davies
Claudio Scazzocchio
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/811,404 external-priority patent/US5198345A/en
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Application granted granted Critical
Publication of ATE161881T1 publication Critical patent/ATE161881T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2422—Alpha-amylase (3.2.1.1.) from plant source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Yarns And Mechanical Finishing Of Yarns Or Ropes (AREA)
  • Mushroom Cultivation (AREA)
  • Looms (AREA)
  • Treatment Of Sludge (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT86902449T 1985-04-15 1986-04-14 Verwendung von promotoren von filamentösen pilzen ATE161881T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA479135 1985-04-15
US06/811,404 US5198345A (en) 1985-04-15 1985-12-20 Vectors in use in filamentous fungi

Publications (1)

Publication Number Publication Date
ATE161881T1 true ATE161881T1 (de) 1998-01-15

Family

ID=25670651

Family Applications (2)

Application Number Title Priority Date Filing Date
AT86902449T ATE161881T1 (de) 1985-04-15 1986-04-14 Verwendung von promotoren von filamentösen pilzen
AT96116202T ATE253119T1 (de) 1985-04-15 1986-04-14 Verwendung des glucoamylasepromotors aus apergillus

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT96116202T ATE253119T1 (de) 1985-04-15 1986-04-14 Verwendung des glucoamylasepromotors aus apergillus

Country Status (6)

Country Link
EP (1) EP0284603B1 (de)
JP (1) JP2868179B2 (de)
AT (2) ATE161881T1 (de)
AU (1) AU606033B2 (de)
DE (2) DE3650664T2 (de)
WO (1) WO1986006097A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
NZ217373A (en) * 1985-08-29 1990-10-26 Genencor Inc Production of heterologous peptide in filamentous fungi, vectors and dna
US6004785A (en) * 1985-08-29 1999-12-21 Genencor International Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
FI872102A7 (fi) * 1986-06-06 1987-12-07 Panlabs Inc Rihmasienten ilmentymisjärjestelmät.
US5447862A (en) * 1987-02-04 1995-09-05 Ciba-Geigy Corporation Pectin lyase genes of aspergillus niger
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
WO1989001969A1 (en) * 1987-09-04 1989-03-09 Novo-Nordisk A/S Process for the production of protein products in aspergillus and promoters for use in aspergillus
US5252726A (en) * 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
ES2063162T5 (es) * 1988-07-28 2002-11-16 Novartis Ag Nuevo sistema de expresion.
US5162228A (en) * 1988-12-28 1992-11-10 Takeda Chemical Industries, Ltd. Gylceraldehyde-3-phosphate dehydrogenase gene and promoter
CA2034487C (en) * 1989-06-16 2000-08-29 Virgil B. Lawlis Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
JP2995770B2 (ja) * 1989-12-15 1999-12-27 吉富製薬株式会社 異種蛋白質の製造方法
WO1991013971A1 (en) * 1990-03-13 1991-09-19 Hawaii Biotechnology Group, Inc. Neurospora expression system
WO1993018166A2 (en) * 1992-03-04 1993-09-16 Genencor International, Inc. PRODUCTION OF $i(ASPERGILLUS NIGER CATALASE-R)
US5382658A (en) * 1992-04-03 1995-01-17 Allelix Biopharmaceuticals Inc. Stability-enhanced variants of parathyroid hormone
CA2098639A1 (en) * 1992-06-19 1993-12-20 K. Anne Kronis Bone stimulating, non-vasoactive parathyroid hormone variants
CA2151154C (en) * 1992-12-10 1999-01-26 William E. Hintz Production of heterologous proteins in filamentous fungi
GB9316883D0 (en) * 1993-08-13 1993-09-29 Univ Leeds Production of heterologous peptices
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
GB9515941D0 (en) 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
WO1996036718A1 (en) * 1995-05-18 1996-11-21 Genencor International, Inc. Expression of glycosyltransferase in aspergillus
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
CA2422748C (en) 2000-09-21 2011-08-09 Dsm N.V. Talaromyces xylanases
EP1464234A1 (de) 2000-12-07 2004-10-06 DSM IP Assets B.V. Verfahren zur Verhinderung oder Verminderung der Trübung in Getränken
CN100506981C (zh) 2000-12-07 2009-07-01 Dsmip资产有限公司 富含具有羧基末端脯氨酸残基的肽的蛋白质水解产物
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
ES2525873T3 (es) 2002-06-07 2014-12-30 Dsm Ip Assets B.V. Método mejorado para la prevención o reducción del enturbiamiento en bebidas
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
CA2630445C (en) 2005-11-28 2016-11-15 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
ES2608033T3 (es) 2007-03-22 2017-04-05 Dsm Ip Assets B.V. Nuevas lisil oxidasas
CN101835801B (zh) 2007-08-08 2014-09-10 诺维信生物制药丹麦公司 转铁蛋白变体和偶联物
WO2010089385A1 (en) 2009-02-06 2010-08-12 Novozymes Biopharma Dk A/S Purification process
JP5936112B2 (ja) 2009-02-11 2016-06-15 アルブミディクス アクティーゼルスカブ アルブミン変異体及び複合体
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0099226A1 (de) * 1982-07-07 1984-01-25 St. Louis University Zur Replikation eines extrachromosomalen Elements befähigte fadenartige Pilze
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
EP0136907A3 (de) * 1983-10-03 1986-12-30 Genentech, Inc. Xenogeneisches Expressionskontrollsystem, Verfahren zu dessen Verwendung, dieses enthaltende Expressionsvektoren, mit denselben transformierte Zellen und daraus hergestellte heterolose Proteine
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
EP0191221A1 (de) * 1984-10-24 1986-08-20 The Regents Of The University Of California Vektoren zur Transformation von Ascomyceten
US4753877A (en) * 1984-11-07 1988-06-28 Penalva Miguel A Cephalosporium acremonium
NZ217373A (en) * 1985-08-29 1990-10-26 Genencor Inc Production of heterologous peptide in filamentous fungi, vectors and dna

Also Published As

Publication number Publication date
DE3650664T2 (de) 1998-04-16
AU5698686A (en) 1986-11-05
WO1986006097A1 (en) 1986-10-23
EP0284603A1 (de) 1988-10-05
EP0284603B1 (de) 1998-01-07
JPH07163389A (ja) 1995-06-27
DE3650783T2 (de) 2004-07-15
ATE253119T1 (de) 2003-11-15
DE3650783D1 (de) 2003-12-04
JP2868179B2 (ja) 1999-03-10
AU606033B2 (en) 1991-01-31
DE3650664D1 (de) 1998-02-12

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Legal Events

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REN Ceased due to non-payment of the annual fee