ATE199391T1 - Expression von an g-protein gekoppelten rezeptoren in hefe - Google Patents

Expression von an g-protein gekoppelten rezeptoren in hefe

Info

Publication number
ATE199391T1
ATE199391T1 AT91916216T AT91916216T ATE199391T1 AT E199391 T1 ATE199391 T1 AT E199391T1 AT 91916216 T AT91916216 T AT 91916216T AT 91916216 T AT91916216 T AT 91916216T AT E199391 T1 ATE199391 T1 AT E199391T1
Authority
AT
Austria
Prior art keywords
protein
segment
alpha
yeast
cell
Prior art date
Application number
AT91916216T
Other languages
English (en)
Inventor
Klim King
Henrik G Dohlman
Marc G Caron
Robert J Lefkowitz
Original Assignee
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24326283&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE199391(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Duke filed Critical Univ Duke
Application granted granted Critical
Publication of ATE199391T1 publication Critical patent/ATE199391T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Neurology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT91916216T 1990-09-13 1991-09-12 Expression von an g-protein gekoppelten rezeptoren in hefe ATE199391T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58171490A 1990-09-13 1990-09-13
PCT/US1991/006605 WO1992005244A1 (en) 1990-09-13 1991-09-12 Expression of g protein coupled receptors in yeast

Publications (1)

Publication Number Publication Date
ATE199391T1 true ATE199391T1 (de) 2001-03-15

Family

ID=24326283

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91916216T ATE199391T1 (de) 1990-09-13 1991-09-12 Expression von an g-protein gekoppelten rezeptoren in hefe

Country Status (12)

Country Link
US (6) US5482835A (de)
EP (2) EP0548165B1 (de)
JP (3) JP3348406B2 (de)
AT (1) ATE199391T1 (de)
AU (1) AU652576B2 (de)
CA (1) CA2092717C (de)
DE (1) DE69132546T2 (de)
DK (1) DK0548165T3 (de)
ES (1) ES2157197T3 (de)
GR (1) GR3035937T3 (de)
IE (2) IE20020110A1 (de)
WO (1) WO1992005244A1 (de)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2628750B1 (fr) * 1988-03-21 1991-11-08 Pasteur Institut Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire
US6080540A (en) 1990-04-20 2000-06-27 Cold Spring Harbor Laboratory Cloning of mammalian genes in microbial organisms and methods for pharmacological screening
JP3348406B2 (ja) 1990-09-13 2002-11-20 デューク ユニバーシティー 酵母におけるｇタンパク質結合受容体の発現
US5264565A (en) * 1991-01-22 1993-11-23 Affymax Technologies, N.V. Nucleic acid encoding the D2 /Ml chimeric receptor
DE4138621A1 (de) * 1991-11-25 1993-06-17 Boehringer Ingelheim Int Verfahren zum screenen von substanzen mit modulierender wirkung auf einen rezeptorabhaengigen zellulaeren signaluebertragungsweg
US5688662A (en) * 1992-04-09 1997-11-18 Linguagen Corporation Gustducin polynucleotides, vectors, host cells and recombinant methods
US6864060B1 (en) 1993-03-31 2005-03-08 Cadus Technologies, Inc. Yeast cells expressing modified G proteins and methods of use therefor
US7416881B1 (en) 1993-03-31 2008-08-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
ATE207118T1 (de) * 1993-03-31 2001-11-15 Cadus Pharmaceutical Corp Hefe zellen so konstruiert, dass sie proteinsurrogate des pheromensystems produzieren und anwendungen dafuer
US6100042A (en) * 1993-03-31 2000-08-08 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US20030054402A1 (en) 1993-03-31 2003-03-20 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US7235648B1 (en) 1993-03-31 2007-06-26 Cadus Technologies, Inc. Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US5789184A (en) * 1993-03-31 1998-08-04 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates, and uses therefor
US5876951A (en) * 1993-03-31 1999-03-02 Cadus Pharmaceutical Corporation Yeast cells engineered to produce pheromone system protein surrogates and uses therefor
US6255059B1 (en) 1993-03-31 2001-07-03 Cadus Pharmaceutical Corporation Methods for identifying G protein coupled receptor effectors
US6001581A (en) * 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US5707798A (en) * 1993-07-13 1998-01-13 Novo Nordisk A/S Identification of ligands by selective amplification of cells transfected with receptors
US5691188A (en) * 1994-02-14 1997-11-25 American Cyanamid Company Transformed yeast cells expressing heterologous G-protein coupled receptor
WO1995030012A1 (en) * 1994-04-26 1995-11-09 Cadus Pharmaceutical Corporation Functional expression of mammalian adenylyl cyclase in yeast
WO1996040939A2 (en) * 1995-06-07 1996-12-19 Cadus Pharmaceutical Corporation Expression of functional vertebrate phospholipases in yeast
AU7115896A (en) * 1995-09-20 1997-04-09 Molecular Geriatrics Corporation Yeast receptor and g-protein fusion protein
US5851786A (en) * 1995-09-27 1998-12-22 National Jewish Center For Immunology And Respiratory Medicine Product and process to regulate actin polymerization
US7329741B2 (en) * 1996-06-05 2008-02-12 Chiron Corporation Polynucleotides that hybridize to DP-75 and their use
WO1997046680A1 (en) 1996-06-05 1997-12-11 Chiron Corporation Dna encoding dp. 75 and a process for its use
US6083693A (en) * 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
DE69731960T2 (de) * 1996-09-24 2005-12-15 Cadus Pharmaceutical Corp. Verfahren und zusammensetzungen für die identifizierung von receptor effectoren
US6087115A (en) * 1997-01-22 2000-07-11 Cornell Research Foundation, Inc. Methods of identifying negative antagonists for G protein coupled receptors
US6403305B1 (en) * 1997-02-06 2002-06-11 Cornell Research Foundation, Inc. Methods of identifying peptide agonists or negative antagonists of a G protein coupled receptor
US5891646A (en) * 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US6528271B1 (en) 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
EP1021538A1 (de) * 1997-10-07 2000-07-26 Cadus Pharmaceutical Corporation Modifizierte g proteine-exprimierende hefezellen und ihre verwendungen
AU9794598A (en) * 1997-10-10 1999-05-03 Lxr Biotechnology Inc. Methods for detecting compounds which modulate the activity of an lpa receptor
US6485922B1 (en) 1997-10-10 2002-11-26 Atairgin Technologies, Inc. Methods for detecting compounds which modulate the activity of an LPA receptor
US6265174B1 (en) 1997-11-03 2001-07-24 Morphochem, Inc. Methods and compositions for identifying and modulating ctionprotein-interactions
GB9725462D0 (en) * 1997-12-01 1998-01-28 Smithkline Beecham Plc Novel method
US5955575A (en) * 1997-12-22 1999-09-21 Hopital Sainte-Justine Antagonists of G-protein-coupled receptor
US7262280B1 (en) 1998-04-03 2007-08-28 Nps Pharmaceuticals, Inc. G-protein fusion receptors and constructs encoding same
CA2325334A1 (en) * 1998-04-03 1999-10-14 Nps Pharmaceuticals, Inc. G-protein fusion receptors and chimeric gabab receptors
GB9807722D0 (en) * 1998-04-08 1998-06-10 Smithkline Beecham Plc Novel compounds
JP2002513135A (ja) 1998-04-24 2002-05-08 エール ユニヴァーシティ 薬剤−受容体及びタンパク質−タンパク質相互作用を検出する方法
US6355473B1 (en) 1998-05-06 2002-03-12 Cadus Pharmaceutical Corp. Yeast cells having mutations in stp22 and uses therefor
US6746852B1 (en) 1998-05-08 2004-06-08 Osi Pharmaceuticals, Inc. AGS proteins and nucleic acid molecules and uses thereof
US6733991B1 (en) * 1998-05-08 2004-05-11 Osi Pharmaceuticals, Inc. AGS proteins and nucleic acid molecules and uses therefor
US6280934B1 (en) 1998-05-13 2001-08-28 Millennium Pharmaceuticals, Inc. Assay for agents which alter G-protein coupled receptor activity
EP1084231A1 (de) * 1998-06-05 2001-03-21 Discovery Technologies Ltd. Transformierte zell-linien, die heterologe g-protein-gekoppelte rezeptoren exprimieren
AU4820399A (en) * 1998-06-29 2000-01-17 Hyseq, Inc. A chemokine receptor obtained from a cdna library of fetal liver-spleen
US7081360B2 (en) 1998-07-28 2006-07-25 Cadus Technologies, Inc. Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties
US7273747B2 (en) 1998-08-27 2007-09-25 Cadus Technologies, Inc. Cell having amplified signal transduction pathway responses and uses therefor
US7094593B1 (en) * 1998-09-01 2006-08-22 Basf Aktiengesellschaft Method for improving the function of heterologous G protein-coupled receptors
US6251605B1 (en) 1998-10-27 2001-06-26 Cadus Pharmaceutical Corporation Yeast cells having mutations in Cav1 and uses therefor
GB9824682D0 (en) * 1998-11-10 1999-01-06 Isis Innovation Bacillus strain and assay method
AU2030000A (en) 1998-11-25 2000-06-13 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
US6074845A (en) * 1999-01-28 2000-06-13 Smithkline Beecham Corporation Nucleic acid encoding a bovine calcitonin receptor-like receptor (BECRLR)
US6504008B1 (en) 1999-02-01 2003-01-07 Cadus Technologies, Inc. Cell based signal generation
US6555325B1 (en) 1999-06-14 2003-04-29 Cadus Technologies, Inc. System for detection of a functional interaction between a compound and a cellular signal transduction component
US7223533B2 (en) 1999-09-10 2007-05-29 Cadus Technologies, Inc. Cell surface proteins and use thereof as indicators of activation of cellular signal transduction pathways
US6998255B1 (en) * 1999-09-24 2006-02-14 Solvay Pharmaceuticals B.V. Human G-protein coupled receptor
WO2001025269A2 (en) 1999-09-24 2001-04-12 Solvay Pharmaceuticals B.V. Human g-protein coupled receptor
US20050148018A1 (en) 1999-10-07 2005-07-07 David Weiner Methods of identifying inverse agonists of the serotonin 2A receptor
AU3263101A (en) * 1999-11-24 2001-06-04 Millennium Pharmaceuticals, Inc. Expression cloning method
US7186505B1 (en) 1999-11-29 2007-03-06 Stil Biotechnologies Ltd. Expression systems and methods for detecting and isolating polypeptides regulating signal transduction pathways
US7324236B2 (en) * 2000-02-01 2008-01-29 Canon Kabushiki Kaisha Discrepancy correction method and apparatus for correcting difference in levels of image signals obtained by an image sensor having a multiple output channels
US20050136431A1 (en) * 2000-11-03 2005-06-23 Oakley Robert H. Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7163800B2 (en) * 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7018812B2 (en) * 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
US7223550B2 (en) * 2001-02-08 2007-05-29 Temple University-Of The Commonwealth System Of Higher Education Biosensor for defecting chemical agents
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
WO2002096946A1 (en) * 2001-05-30 2002-12-05 Bristol-Myers Squibb Company Polynucleotide encoding a novel human g-protein coupled receptor, hgprbmy30
US20030232359A1 (en) * 2002-03-18 2003-12-18 Ramanathan Chandra S. Polynucleotide encoding a novel human G-protein coupled receptor, HGPRBMY40_2
US20030182669A1 (en) * 2002-03-19 2003-09-25 Rockman Howard A. Phosphoinositide 3-kinase mediated inhibition of GPCRs
US7290215B2 (en) * 2002-06-03 2007-10-30 Microsoft Corporation Dynamic wizard interface system and method
WO2004079000A1 (en) * 2003-03-07 2004-09-16 National Research Council Of Canada The determination of function of mammalian proteins by analysis of global gene expression profiles
US20050186658A1 (en) * 2003-10-17 2005-08-25 Nps Pharmaceuticals, Inc. Chimeric metabotropic glutamate receptors and uses thereof
EP2497478A3 (de) 2003-12-05 2012-11-07 Children's Hospital Medical Center Oligosaccarid-Zusammensetzungen und ihre Verwendung bei der Behandlung von Infektionen
JP2008537481A (ja) 2005-03-07 2008-09-18 ユニヴァーシティー オブ ロチェスター Ｇタンパク質シグナリングを阻害する組成物及び方法
GB0520568D0 (en) 2005-10-10 2005-11-16 Paradigm Therapeutics Ltd Receptor
CN109071626A (zh) 2016-02-24 2018-12-21 阿罗姆斯公司 用于检测气味、香味和味道的生物传感器
US10209239B1 (en) 2017-08-16 2019-02-19 Aromyx Corporation Method of making an aromagraph comprising ectopic olfactory receptors

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU560472B2 (en) * 1981-08-25 1987-04-09 Celltech Limited Yeast expression vectors
US4865989A (en) * 1981-12-22 1989-09-12 U.C. Regents Eukaryotic vectors and plasmids having PGK regulatory signals
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
US4661454A (en) 1983-02-28 1987-04-28 Collaborative Research, Inc. GAL1 yeast promoter linked to non galactokinase gene
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4797359A (en) * 1983-05-10 1989-01-10 Board Of Regents, The University Of Texas System Heat shock regulated production of selected and fused proteins in yeast
US4880734A (en) * 1984-05-11 1989-11-14 Chiron Corporation Eukaryotic regulatable transcription
US4859609A (en) 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
US5071773A (en) 1986-10-24 1991-12-10 The Salk Institute For Biological Studies Hormone receptor-related bioassays
US5378603A (en) 1987-03-30 1995-01-03 Board Of Regents, The University Of Texas System Method and composition for identifying substances which activate transcription of the LDL receptor gene
US4952499A (en) 1988-02-11 1990-08-28 Dana-Farber Cancer Institute Genes and their encoded proteins which regulate gene expression of the interleukin-2 receptor and of human lymphotropic retroviruses
FR2628750B1 (fr) * 1988-03-21 1991-11-08 Pasteur Institut Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire
US5386025A (en) 1990-02-20 1995-01-31 The Salk Institute Biotechnology/Industrial Associates Calcium channel compositions and methods
FI904827A7 (fi) 1988-04-04 1990-10-01 The Salk Inst Biotechnology/ Kalsiumkanavakoostumuksia ja -menetelmiä
US5407820A (en) 1988-04-04 1995-04-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Calcium channel α-2 subunit DNAs and cells expressing them
US5747336A (en) 1988-09-08 1998-05-05 The United States Of America As Represented By The Department Of Health And Human Services Cloned human genes for muscarinic acetylcholine receptors and cells lines expressing same
WO1990005780A1 (en) * 1988-11-18 1990-05-31 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education, Acting For And On Behalf Of The Oregon Health Sciences University Dopamine receptors and genes
US5217867A (en) * 1988-11-30 1993-06-08 The Salk Institute For Biological Studies Receptors: their identification, characterization, preparation and use
EP0483249B1 (de) 1989-07-18 2003-04-23 OSI Pharmaceuticals, Inc. Methode der veränderung der expression von genen bei der transkription und zum nachweis von chemischen substanzen, die wie modulatoren der genexpression wirken
US5665543A (en) 1989-07-18 1997-09-09 Oncogene Science, Inc. Method of discovering chemicals capable of functioning as gene expression modulators
US5284746A (en) 1990-02-08 1994-02-08 Zymogenetics, Inc. Methods of producing hybrid G protein-coupled receptors
US5369028A (en) 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
AU7686891A (en) * 1990-04-05 1991-10-30 American National Red Cross, The A protein family related to immediate-early protein expressed by human endothelial cells during differentiation
AU8066591A (en) * 1990-05-14 1991-12-10 Duke University Cloned genes encoding the d1 dopamine receptor
US5426177A (en) 1990-06-01 1995-06-20 Regeneron Pharmaceuticals, Inc. Ciliary neurotrophic factor receptor
US5401629A (en) * 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
JP3348406B2 (ja) * 1990-09-13 2002-11-20 デューク ユニバーシティー 酵母におけるｇタンパク質結合受容体の発現
NZ240921A (en) * 1990-12-12 1994-06-27 Zymogenetics Inc G protein coupled glutamate receptor (neurotransmitters), recombinant production
US5384243A (en) * 1991-04-12 1995-01-24 The United States Of America As Represented By The Department Of Health And Human Services Method for screening an agent for its ability to prevent cell transformation
US5245011A (en) * 1991-04-16 1993-09-14 Duke University Aqueous solution containing D1B dopamine receptor
US5215915A (en) * 1991-04-16 1993-06-01 Duke University Cloned gene encoding rat d1b dopamine receptor
WO1992019723A1 (en) * 1991-04-29 1992-11-12 The Trustees Of Boston University Method of isolating ligands which bind neutrophil receptors
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
US5364791A (en) * 1992-05-14 1994-11-15 Elisabetta Vegeto Progesterone receptor having C. terminal hormone binding domain truncations
US5468615A (en) * 1993-07-01 1995-11-21 The Upjohn Company Binding assay employing a synthetic gene for D4 dopamine receptors

Also Published As

Publication number Publication date
ES2157197T3 (es) 2001-08-16
US6855550B2 (en) 2005-02-15
DK0548165T3 (da) 2001-07-16
US20050214739A1 (en) 2005-09-29
AU652576B2 (en) 1994-09-01
US7413876B2 (en) 2008-08-19
GR3035937T3 (en) 2001-08-31
JP2002253282A (ja) 2002-09-10
CA2092717C (en) 2001-06-12
US5739029A (en) 1998-04-14
EP0548165A4 (en) 1993-08-11
AU8511591A (en) 1992-04-15
DE69132546T2 (de) 2001-10-04
JP3348406B2 (ja) 2002-11-20
EP0548165B1 (de) 2001-02-28
WO1992005244A1 (en) 1992-04-02
EP0548165A1 (de) 1993-06-30
DE69132546D1 (de) 2001-04-05
CA2092717A1 (en) 1992-03-14
US20020022238A1 (en) 2002-02-21
IE913242A1 (en) 1992-02-25
US5482835A (en) 1996-01-09
US20030215887A1 (en) 2003-11-20
US6168927B1 (en) 2001-01-02
JPH06500693A (ja) 1994-01-27
EP1029915A3 (de) 2004-11-17
JP2004000282A (ja) 2004-01-08
EP1029915A2 (de) 2000-08-23
IE20020110A1 (en) 2003-05-28

Similar Documents

Publication Publication Date Title
DE69132546D1 (de) Expression von an g-protein gekoppelten rezeptoren in hefe
ES2172502T3 (es) Receptores tipo ii de interleucina 1.
DE69024291D1 (de) Rezeptoren für Tumornekrosisfaktor-alpha und -beta
NO931955L (no) Reseptorer for leukemiinhiberende faktor
DK144695A (da) Ekspressionsvektor eller et par af ekspressionsvektorer omfattende heterolog DNA, som koder for alfa- og beta-underenhederne af humant FSH, eukatyotisk celle transficeret med vektoren eller parret af vektorer, fremgangsmåde til fremstilling af biologisk aktivt heterodimert humant FSH og fremgangsmåde til fremstilling af et farmaceutisk præparat indeholdende rekombinant humant FSH
ATE153068T1 (de) Interleukin-4-rezeptoren
DE69232537D1 (de) Rezeptoren der steroid/thyroid superfamilie von rezeptoren
PT96714A (pt) Processo para a preparacao de receptores acoplados a proteina g hibrida
AU1846995A (en) Heterologous g protein coupled receptors expressed in yeast, their fusion with g proteins and use thereof in bioassay
NO2006009I1 (no) Natalizumab eller farmasoytisk akseptable salter derav
ES2036192T3 (es) Derivados del acido (met)-acrilico, que contienen grupos uretano, de triciclo (5.2.1.0.2.6) decano.
KR880700858A (ko) 재조합된 인간의 내피성 세포의 생장인자
ES2061582T3 (es) Procedimiento para producir una proteina de factor viii:c humano recombinante.
GR80517B (en) Cdna clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity
DK0479948T3 (da) Sammensætninger og fremgangsmåder til syntese af natriuretisk protein-receptor B og anvendelsesfremgangsmåder
HU901326D0 (en) Recombinant protein receptor and process for production thereof
DE59611434D1 (de) Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
DK308586D0 (da) Dna-sekvens, der koder for et specifikt protein, rekombinantvektor, der omfatter sekvensen, og celler, der er transficerede til ekspression af proteinet
EP0749441A4 (de) Expression und reinigung von kloniertem humanen alpha-fetoprotein
DK432987A (da) Fremgangsmaade til forbedring af ekspressionen af eukaryotprotein
MX9705172A (es) Polipeptido asociado a il-2r y moleculas de adn que codifican el mismo.
EP0494702A3 (en) Production of factor xiiia by gene technology
ATE148499T1 (de) Herstellung monoklonaler antikörper gegen menschliches vbeta t-zellrezeptorelement unter verwendung rekombinanter dna-vektoren
EP0155192A3 (de) CDNS-Klonen kodierend für eine IgE-Bindungsfaktoraktivität aufweisende Polypeptide
King et al. Methods of testing in yeast cells for agonists and antagonists of mammal G-protein coupled receptors

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee