ATE206465T1 - Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien - Google Patents

Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien

Info

Publication number
ATE206465T1
ATE206465T1 AT95103520T AT95103520T ATE206465T1 AT E206465 T1 ATE206465 T1 AT E206465T1 AT 95103520 T AT95103520 T AT 95103520T AT 95103520 T AT95103520 T AT 95103520T AT E206465 T1 ATE206465 T1 AT E206465T1
Authority
AT
Austria
Prior art keywords
enzyme
ionic detergent
inhibition
suppressing
reaction
Prior art date
Application number
AT95103520T
Other languages
English (en)
Inventor
Daniel Louis Kacian
Diane Lisa Mcallister
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22789688&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE206465(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE206465T1 publication Critical patent/ATE206465T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
AT95103520T 1994-03-10 1995-03-10 Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien ATE206465T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21213194A 1994-03-10 1994-03-10

Publications (1)

Publication Number Publication Date
ATE206465T1 true ATE206465T1 (de) 2001-10-15

Family

ID=22789688

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95103520T ATE206465T1 (de) 1994-03-10 1995-03-10 Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien

Country Status (11)

Country Link
US (3) US5766890A (de)
EP (1) EP0671473B1 (de)
JP (1) JP3062250B2 (de)
KR (1) KR100238833B1 (de)
AT (1) ATE206465T1 (de)
AU (1) AU699232B2 (de)
CA (1) CA2184270C (de)
DE (1) DE69522978T2 (de)
DK (1) DK0671473T3 (de)
ES (1) ES2162875T3 (de)
WO (1) WO1995024499A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
JP2000500342A (ja) 1995-11-15 2000-01-18 ジェン−プローブ・インコーポレーテッド ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキット
US5800997A (en) * 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
US6558901B1 (en) 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
GB9922971D0 (en) 1999-09-29 1999-12-01 Secr Defence Reaction system
US6632557B1 (en) * 1999-10-26 2003-10-14 The Gillette Company Cathodes for metal air electrochemical cells
WO2001042456A2 (en) * 1999-12-10 2001-06-14 Genespan Corporation Isolation and purification of nucleic acids
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
WO2002014548A1 (en) * 2000-08-10 2002-02-21 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
GB0110476D0 (en) * 2001-04-30 2001-06-20 Secr Defence Reagent delivery system
DE60330842D1 (de) 2002-01-28 2010-02-25 Ambion Inc Für den nachweis von nukleinsäuren kompetente biologische rohderivate
EP1558748A4 (de) 2002-11-01 2008-10-22 Promega Corp Zellysezusammensetzungen, verwendungsverfahren, vorrichtung und kit
EP2042612B1 (de) * 2004-01-23 2010-12-15 bioMerieux, Inc. Neuartiges Nukleotidgemisch für eine verbesserte Nukleinsäurenamplifikationsleistung
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1812601A2 (de) 2004-10-22 2007-08-01 Redpath Integrated Pathology, Inc. Dynamische expansion genomischer deletion und formulierung aus molekularmarkerpanels für integrierte molekulare pathologiediagnose und charakterisierung von gewebe, zellflüssigkeit und reinen flüssigproben
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
EP2348320B1 (de) 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
US7972828B2 (en) 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
WO2008122088A1 (en) * 2007-04-05 2008-10-16 Lifeprint Australia Pty Ltd Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
CN101679957A (zh) * 2007-06-13 2010-03-24 通用电气医疗集团生物科学公司 聚合酶稳定
JP5546109B2 (ja) * 2008-05-27 2014-07-09 富士フイルム株式会社 核酸の塩基配列の識別方法
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
EP2955233A1 (de) * 2008-12-19 2015-12-16 Life Technologies Corporation Proteinase K Inhibitore, Verfahren und Zusammensetzungen davon
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
WO2013050881A2 (en) * 2011-10-05 2013-04-11 Spartan Bioscience Inc. Direct nucleic acid analysis
AU2013202804A1 (en) 2012-06-14 2014-01-16 Gen-Probe Incorporated Use of a fluorescent material to detect failure or deteriorated performance of a fluorometer
US9341628B2 (en) 2012-11-30 2016-05-17 Interpace Diagnostics Corporation Methods for measuring carcinoembryonic antigen
EP3063129B1 (de) 2013-10-25 2019-04-17 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
CN107109402B (zh) * 2014-12-23 2020-09-25 3M创新有限公司 用于减少核酸扩增抑制的组合物
CN108350407B (zh) 2015-12-11 2022-07-08 杰诺玛迪克斯公司 用于核酸扩增的管密封系统和方法
CA3008891C (en) 2015-12-31 2024-02-20 Gen-Probe Incorporated Systems and methods for analyzing a sample and for monitoring the performance of an optical signal detector
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
EP3645710A1 (de) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophile dna-polymerase-mutanten
WO2022021163A1 (zh) * 2020-07-29 2022-02-03 深圳华大智造科技股份有限公司 在固体支持物上装载核酸分子的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8722252D0 (en) * 1987-09-22 1987-10-28 Brewing Res Found Atp extraction
US5358690A (en) * 1989-01-10 1994-10-25 Lamina, Ltd. Environmental sample collection and membrane testing device
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
EP0488243A1 (de) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Verfahren zur Extraktion des viralen Genoms von einer von einem lebenden Körper abgeleiteten Probe, die mit dem Virus infiziert ist sowie Nachweis des Genoms
US5175094A (en) * 1991-08-01 1992-12-29 Becton Dickinson And Company Increased expression of HBcAg
JPH08501208A (ja) * 1992-06-12 1996-02-13 ジェン−プローブ・インコーポレイテッド 血液から核酸の製造
CA2161337A1 (en) * 1993-05-06 1994-11-24 Denis R. Henrard Direct lysis buffer and the detection of hiv-1 plasma viremia
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor

Also Published As

Publication number Publication date
EP0671473B1 (de) 2001-10-04
DK0671473T3 (da) 2001-10-29
WO1995024499A1 (en) 1995-09-14
AU2097895A (en) 1995-09-25
KR970701787A (ko) 1997-04-12
US5846701A (en) 1998-12-08
DE69522978T2 (de) 2002-04-04
ES2162875T3 (es) 2002-01-16
AU699232B2 (en) 1998-11-26
CA2184270A1 (en) 1995-09-14
US5766890A (en) 1998-06-16
JP3062250B2 (ja) 2000-07-10
US5871975A (en) 1999-02-16
EP0671473A1 (de) 1995-09-13
JPH09510099A (ja) 1997-10-14
DE69522978D1 (de) 2001-11-08
CA2184270C (en) 1999-08-17
KR100238833B1 (ko) 2000-03-02

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