DK0671473T3 - Fremgangsmåde til undertrykkelse af inhibering af enzymmedierede reaktioner med ioniske detergenter - Google Patents
Fremgangsmåde til undertrykkelse af inhibering af enzymmedierede reaktioner med ioniske detergenterInfo
- Publication number
- DK0671473T3 DK0671473T3 DK95103520T DK95103520T DK0671473T3 DK 0671473 T3 DK0671473 T3 DK 0671473T3 DK 95103520 T DK95103520 T DK 95103520T DK 95103520 T DK95103520 T DK 95103520T DK 0671473 T3 DK0671473 T3 DK 0671473T3
- Authority
- DK
- Denmark
- Prior art keywords
- enzyme
- ionic detergent
- reaction
- ionic detergents
- mediated reactions
- Prior art date
Links
- 239000003599 detergent Substances 0.000 title abstract 5
- 238000006243 chemical reaction Methods 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 230000005764 inhibitory process Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000001404 mediated effect Effects 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21213194A | 1994-03-10 | 1994-03-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0671473T3 true DK0671473T3 (da) | 2001-10-29 |
Family
ID=22789688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK95103520T DK0671473T3 (da) | 1994-03-10 | 1995-03-10 | Fremgangsmåde til undertrykkelse af inhibering af enzymmedierede reaktioner med ioniske detergenter |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US5846701A (da) |
| EP (1) | EP0671473B1 (da) |
| JP (1) | JP3062250B2 (da) |
| KR (1) | KR100238833B1 (da) |
| AT (1) | ATE206465T1 (da) |
| AU (1) | AU699232B2 (da) |
| CA (1) | CA2184270C (da) |
| DE (1) | DE69522978T2 (da) |
| DK (1) | DK0671473T3 (da) |
| ES (1) | ES2162875T3 (da) |
| WO (1) | WO1995024499A1 (da) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5514551A (en) * | 1994-10-14 | 1996-05-07 | Gen-Probe Incorporated | Compositions for the detection of Chlamydia trachomatis |
| CA2237891C (en) * | 1995-11-15 | 2013-07-30 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| US5800997A (en) * | 1996-11-01 | 1998-09-01 | Novartis Finance Corporation | Detection of maize fungal pathogens using the polymerase chain reaction |
| IL124275A (en) * | 1997-05-02 | 2002-03-10 | Bio Merieux Vitek Inc | A method to produce sequences of nucleic acids |
| US6558901B1 (en) | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
| EP0989192A3 (en) * | 1998-09-21 | 2004-02-25 | Shimadzu Corporation | Method for synthesis of nucleic acids |
| US6242188B1 (en) | 1999-07-30 | 2001-06-05 | Applied Gene Technologies, Inc. | Sample processing to release nucleic acids for direct detection |
| GB9922971D0 (en) * | 1999-09-29 | 1999-12-01 | Secr Defence | Reaction system |
| US6632557B1 (en) * | 1999-10-26 | 2003-10-14 | The Gillette Company | Cathodes for metal air electrochemical cells |
| WO2001042456A2 (en) * | 1999-12-10 | 2001-06-14 | Genespan Corporation | Isolation and purification of nucleic acids |
| US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| AU2000267686A1 (en) * | 2000-08-10 | 2002-02-25 | Applied Gene Technologies, Inc. | Compositions and methods for nucleic acids sample processing and amplification |
| GB0110476D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence | Reagent delivery system |
| EP2290098A1 (en) | 2002-01-28 | 2011-03-02 | Ambion, Inc. | Preparing crude biological extracts using protease, suitable for preparing cDNA |
| US7319021B2 (en) | 2002-11-01 | 2008-01-15 | Promega Corporation | Cell lysis composition, methods of use, apparatus and kit |
| ATE491810T1 (de) * | 2004-01-23 | 2011-01-15 | Bio Merieux Inc | Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung |
| US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
| WO2006047483A2 (en) * | 2004-10-22 | 2006-05-04 | Redpath Integrated Pathology, Inc. | Dynamic genomic deletion expansion and formulation of molecular marker panels for integrated molecular pathology diagnosis and characterization of tissue, cellular fluid, and pure fluid specimens |
| FR2878254B1 (fr) * | 2004-11-22 | 2010-08-20 | Bio Rad Pasteur | Composition pour l'amplification d'acides nucleiques |
| US7964413B2 (en) | 2005-03-10 | 2011-06-21 | Gen-Probe Incorporated | Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay |
| EP2348320B1 (en) | 2005-03-10 | 2024-05-01 | Gen-Probe Incorporated | Methods and systems for detecting multiple fluorescent emission signals |
| US7972828B2 (en) * | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
| US9181554B2 (en) * | 2007-04-05 | 2015-11-10 | Australian Centre for Plant Functional Genomics Pty, Ltd | Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex |
| US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
| BRPI0813342A2 (pt) * | 2007-06-13 | 2014-10-14 | Ge Healthcare Bio Sciences | " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ". |
| JP5546109B2 (ja) * | 2008-05-27 | 2014-07-09 | 富士フイルム株式会社 | 核酸の塩基配列の識別方法 |
| US8097412B2 (en) * | 2008-07-12 | 2012-01-17 | Biodiagnostics, Inc. | DNA-based test for detection of annual and intermediate ryegrass |
| EP2367954B1 (en) * | 2008-12-19 | 2015-02-18 | Life Technologies Corporation | Proteinase k inhibitors, methods and compositions therefor |
| EP2678664B1 (en) | 2011-02-24 | 2019-08-07 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| FI3461807T3 (fi) | 2011-06-08 | 2023-09-07 | Life Technologies Corp | Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten |
| EP2764112B1 (en) * | 2011-10-05 | 2018-04-18 | Spartan Bioscience Inc. | Direct nucleic acid analysis |
| AU2013202804A1 (en) | 2012-06-14 | 2014-01-16 | Gen-Probe Incorporated | Use of a fluorescent material to detect failure or deteriorated performance of a fluorometer |
| US9341628B2 (en) | 2012-11-30 | 2016-05-17 | Interpace Diagnostics Corporation | Methods for measuring carcinoembryonic antigen |
| EP3539944A1 (en) | 2013-10-25 | 2019-09-18 | Life Technologies Corporation | Novel compounds for use in pcr systems and applications thereof |
| EP3237637B1 (en) * | 2014-12-23 | 2019-09-04 | 3M Innovative Properties Company | Composition for reducing inhibition of nucleic acid amplification |
| US10391498B2 (en) | 2015-12-11 | 2019-08-27 | Spartan Bioscience Inc. | Systems and methods for nucleic acid amplification |
| JP6845858B2 (ja) | 2015-12-31 | 2021-03-24 | ジェン−プローブ・インコーポレーテッド | サンプルを分析し、光信号検出器の性能を監視するシステム及び方法 |
| US10626383B2 (en) | 2016-01-15 | 2020-04-21 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| JP7620080B2 (ja) * | 2020-07-29 | 2025-01-22 | 深▲セン▼華大智造科技股▲ふん▼有限公司 | 核酸分子を固体支持体に載置するための方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8722252D0 (en) * | 1987-09-22 | 1987-10-28 | Brewing Res Found | Atp extraction |
| US5358690A (en) * | 1989-01-10 | 1994-10-25 | Lamina, Ltd. | Environmental sample collection and membrane testing device |
| US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
| US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
| EP0488243A1 (en) * | 1990-11-30 | 1992-06-03 | Sanwa Kagaku Kenkyusho Co., Ltd. | Method of extracting virus genome from sample derived from living body infected with the virus and detecting the genome |
| US5175094A (en) * | 1991-08-01 | 1992-12-29 | Becton Dickinson And Company | Increased expression of HBcAg |
| AU668746B2 (en) * | 1992-06-12 | 1996-05-16 | Gen-Probe Incorporated | Preparation of nucleic acid from blood |
| EP0698082A4 (en) * | 1993-05-06 | 1997-12-17 | Abbott Lab | A BUFFER FOR DIRECT LYSIS AND DETECTING HIV-1 PLASMA VIREMIA |
| US5538870A (en) * | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
-
1995
- 1995-03-07 AU AU20978/95A patent/AU699232B2/en not_active Ceased
- 1995-03-07 JP JP7523607A patent/JP3062250B2/ja not_active Expired - Fee Related
- 1995-03-07 KR KR1019960704966A patent/KR100238833B1/ko not_active Expired - Fee Related
- 1995-03-07 CA CA002184270A patent/CA2184270C/en not_active Expired - Fee Related
- 1995-03-07 WO PCT/US1995/002865 patent/WO1995024499A1/en not_active Ceased
- 1995-03-10 ES ES95103520T patent/ES2162875T3/es not_active Expired - Lifetime
- 1995-03-10 DK DK95103520T patent/DK0671473T3/da active
- 1995-03-10 EP EP95103520A patent/EP0671473B1/en not_active Expired - Lifetime
- 1995-03-10 DE DE69522978T patent/DE69522978T2/de not_active Expired - Lifetime
- 1995-03-10 AT AT95103520T patent/ATE206465T1/de not_active IP Right Cessation
-
1997
- 1997-01-06 US US08/779,342 patent/US5846701A/en not_active Expired - Lifetime
- 1997-01-06 US US08/779,341 patent/US5766890A/en not_active Expired - Fee Related
- 1997-03-25 US US08/823,568 patent/US5871975A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU699232B2 (en) | 1998-11-26 |
| JPH09510099A (ja) | 1997-10-14 |
| US5871975A (en) | 1999-02-16 |
| ES2162875T3 (es) | 2002-01-16 |
| DE69522978D1 (de) | 2001-11-08 |
| EP0671473A1 (en) | 1995-09-13 |
| US5766890A (en) | 1998-06-16 |
| JP3062250B2 (ja) | 2000-07-10 |
| DE69522978T2 (de) | 2002-04-04 |
| CA2184270A1 (en) | 1995-09-14 |
| ATE206465T1 (de) | 2001-10-15 |
| KR100238833B1 (ko) | 2000-03-02 |
| KR970701787A (ko) | 1997-04-12 |
| CA2184270C (en) | 1999-08-17 |
| US5846701A (en) | 1998-12-08 |
| WO1995024499A1 (en) | 1995-09-14 |
| AU2097895A (en) | 1995-09-25 |
| EP0671473B1 (en) | 2001-10-04 |
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