ATE207124T1 - Für das yap3 signalpeptid kodierendes dna- konstrukt - Google Patents

Für das yap3 signalpeptid kodierendes dna- konstrukt

Info

Publication number
ATE207124T1
ATE207124T1 AT94920419T AT94920419T ATE207124T1 AT E207124 T1 ATE207124 T1 AT E207124T1 AT 94920419 T AT94920419 T AT 94920419T AT 94920419 T AT94920419 T AT 94920419T AT E207124 T1 ATE207124 T1 AT E207124T1
Authority
AT
Austria
Prior art keywords
pct
signal peptide
sequence encoding
dna sequence
yap3
Prior art date
Application number
AT94920419T
Other languages
English (en)
Inventor
Lars Christiansen
Jens G Litske Petersen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE207124T1 publication Critical patent/ATE207124T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT94920419T 1993-07-08 1994-07-08 Für das yap3 signalpeptid kodierendes dna- konstrukt ATE207124T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK93828A DK82893D0 (da) 1993-07-08 1993-07-08 Peptid
PCT/DK1994/000281 WO1995002059A1 (en) 1993-07-08 1994-07-08 A dna construct encoding the yap3 signal peptide

Publications (1)

Publication Number Publication Date
ATE207124T1 true ATE207124T1 (de) 2001-11-15

Family

ID=8098010

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94920419T ATE207124T1 (de) 1993-07-08 1994-07-08 Für das yap3 signalpeptid kodierendes dna- konstrukt

Country Status (20)

Country Link
US (1) US5726038A (de)
EP (1) EP0792367B1 (de)
JP (1) JP3542604B2 (de)
KR (1) KR100245173B1 (de)
AT (1) ATE207124T1 (de)
AU (1) AU7122194A (de)
CA (1) CA2166684A1 (de)
DE (1) DE69428719T2 (de)
DK (2) DK82893D0 (de)
ES (1) ES2165881T3 (de)
HU (1) HU222406B1 (de)
IL (1) IL110238A (de)
NZ (1) NZ268346A (de)
PL (1) PL178924B1 (de)
PT (1) PT792367E (de)
RU (1) RU2143495C1 (de)
SG (1) SG52452A1 (de)
UA (1) UA40625C2 (de)
WO (1) WO1995002059A1 (de)
ZA (1) ZA944912B (de)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5772629A (en) * 1995-10-23 1998-06-30 Localmed, Inc. Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP0948538B1 (de) 1996-12-13 2008-06-11 Novartis Vaccines and Diagnostics, Inc. Analyse und separation von pdgf-proteinen
EP0954589A1 (de) * 1997-01-24 1999-11-10 Novo Nordisk A/S Sythetische signalpeptid-sequenzen.
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
JP2003531567A (ja) * 1999-04-30 2003-10-28 オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド チモーゲン活性化系
DE60044728D1 (de) 1999-12-29 2010-09-02 Novo Nordisk As VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE
EP1803730A1 (de) * 2000-04-12 2007-07-04 Human Genome Sciences, Inc. Albuminfusionsproteine
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US20030143191A1 (en) * 2001-05-25 2003-07-31 Adam Bell Chemokine beta-1 fusion proteins
EP1997829A1 (de) * 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Albumin Fusionsproteine
CA2484556A1 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
ATE480624T1 (de) * 2002-06-29 2010-09-15 Korea Res Inst Of Bioscience Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
DE60335497D1 (de) 2002-09-13 2011-02-03 Cornell Res Foundation Inc Ergillus-phytasen
KR100470978B1 (ko) * 2003-01-02 2005-03-10 한국생명공학연구원 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2005047508A1 (en) 2003-11-14 2005-05-26 Novo Nordisk A/S Processes for making acylated insulin
JP5697831B2 (ja) 2003-12-03 2015-04-08 ノヴォ ノルディスク アー／エス 単鎖インシュリン
HUE027902T2 (en) * 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
ATE513921T1 (de) 2005-08-16 2011-07-15 Novo Nordisk As Verfahren zur herstellung reifer insulinpolypeptide
ATE482977T1 (de) 2006-02-27 2010-10-15 Novo Nordisk As Insulinderivate
EP2069502B1 (de) 2006-09-27 2014-02-26 Novo Nordisk A/S Verfahren zur herstellung gereifter insulinpolypeptide
JP5404411B2 (ja) 2006-11-22 2014-01-29 ノボ・ノルデイスク・エー／エス 活性化カルボキシペプチダーゼの製造方法
JP5688969B2 (ja) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
PT2910570T (pt) 2008-03-18 2017-01-24 Novo Nordisk As Análogos de insulina acilados, estabilizados contra proteases
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
CA2819552C (en) 2010-12-09 2023-09-26 Institut Pasteur Mgmt-based method for obtaining high yield of recombinant protein expression
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
EP2788478B1 (de) 2011-12-09 2017-08-23 Institut Pasteur Multiplex-immunoscreening-test
CA2886460C (en) 2012-10-29 2023-11-14 Lonza Ltd Leader peptides for expressing a protein of interest in an eukaryotic host cell
JP6735561B2 (ja) 2012-12-03 2020-08-05 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ｏ−グリコシル化カルボキシ末端部分（ｃｔｐ）ペプチド系のインスリンおよびインスリン類似体
CN105308067B (zh) 2013-06-07 2020-07-24 诺和诺德股份有限公司 制备成熟胰岛素多肽的方法
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
EP3268384B1 (de) 2015-03-10 2021-11-03 Merck Sharp & Dohme Corp. Verfahren zur herstellung von rekombinantem insulin unter verwendung von mikrofiltration
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
WO2019099615A1 (en) 2017-11-17 2019-05-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of copeptin to modulate the immune response and hematopoiesis, and stimulate bone growth
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2
CN112920280B (zh) * 2021-04-22 2022-07-05 江南大学 一种酸性蛋白酶高效表达的方法及其应用
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

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WO1987002670A1 (en) * 1985-10-25 1987-05-07 Mackay Vivian L Method of using bar1 for secreting foreign proteins
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide

Also Published As

Publication number Publication date
PT792367E (pt) 2002-04-29
RU2143495C1 (ru) 1999-12-27
US5726038A (en) 1998-03-10
DE69428719T2 (de) 2002-08-08
AU7122194A (en) 1995-02-06
ES2165881T3 (es) 2002-04-01
JP3542604B2 (ja) 2004-07-14
DK82893D0 (da) 1993-07-08
EP0792367B1 (de) 2001-10-17
IL110238A0 (en) 1994-10-21
HU9600053D0 (en) 1996-03-28
PL312436A1 (en) 1996-04-29
KR100245173B1 (en) 2000-02-15
DK0792367T3 (da) 2001-12-31
WO1995002059A1 (en) 1995-01-19
JPH08512202A (ja) 1996-12-24
EP0792367A1 (de) 1997-09-03
SG52452A1 (en) 1998-09-28
HUT74253A (en) 1996-11-28
NZ268346A (en) 1997-05-26
ZA944912B (en) 1995-03-27
HU222406B1 (hu) 2003-06-28
PL178924B1 (pl) 2000-06-30
CA2166684A1 (en) 1995-01-19
UA40625C2 (uk) 2001-08-15
IL110238A (en) 2003-04-10
DE69428719D1 (de) 2001-11-22

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee