ATE216729T1 - Eine methode und ein gerät für die durchführung einer vervielfältigung von nukleinsäuren an trägern - Google Patents

Eine methode und ein gerät für die durchführung einer vervielfältigung von nukleinsäuren an trägern

Info

Publication number
ATE216729T1
ATE216729T1 AT95930794T AT95930794T ATE216729T1 AT E216729 T1 ATE216729 T1 AT E216729T1 AT 95930794 T AT95930794 T AT 95930794T AT 95930794 T AT95930794 T AT 95930794T AT E216729 T1 ATE216729 T1 AT E216729T1
Authority
AT
Austria
Prior art keywords
supports
nucleic acids
nucleic acid
performing duplication
methods
Prior art date
Application number
AT95930794T
Other languages
English (en)
Inventor
Christopher P Adams
Stephen Joseph Kron
Original Assignee
Mosaic Technologies Inc
Whitehead Biomedical Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies Inc, Whitehead Biomedical Inst filed Critical Mosaic Technologies Inc
Application granted granted Critical
Publication of ATE216729T1 publication Critical patent/ATE216729T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Conveying And Assembling Of Building Elements In Situ (AREA)
  • Load-Engaging Elements For Cranes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Control Of Non-Electrical Variables (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT95930794T 1994-08-03 1995-08-03 Eine methode und ein gerät für die durchführung einer vervielfältigung von nukleinsäuren an trägern ATE216729T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/285,385 US5641658A (en) 1994-08-03 1994-08-03 Method for performing amplification of nucleic acid with two primers bound to a single solid support
PCT/US1995/009905 WO1996004404A1 (en) 1994-08-03 1995-08-03 Method and apparatus for performing amplification of nucleic acid on supports

Publications (1)

Publication Number Publication Date
ATE216729T1 true ATE216729T1 (de) 2002-05-15

Family

ID=23094009

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95930794T ATE216729T1 (de) 1994-08-03 1995-08-03 Eine methode und ein gerät für die durchführung einer vervielfältigung von nukleinsäuren an trägern

Country Status (7)

Country Link
US (1) US5641658A (de)
EP (1) EP0784701B1 (de)
JP (1) JPH10505492A (de)
AT (1) ATE216729T1 (de)
CA (1) CA2196604A1 (de)
DE (1) DE69526511D1 (de)
WO (1) WO1996004404A1 (de)

Families Citing this family (540)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU707391B2 (en) * 1994-05-28 1999-07-08 Tepnel Medical Limited Producing copies of nucleic acids
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US6468751B1 (en) * 1994-08-03 2002-10-22 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acid on supports
US6090592A (en) * 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
GB9503808D0 (en) * 1995-02-24 1995-04-12 Univ Nottingham Detection assay
EP0867506A1 (de) * 1996-09-13 1998-09-30 Laboratory of Molecular Biophotonics Festphase zur detektion von zielnukleinsäuren, verfahren zu deren herstellung, und methode der detektion
US6361940B1 (en) 1996-09-24 2002-03-26 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization and priming specificity
GB9624165D0 (en) * 1996-11-19 1997-01-08 Amdex A S Use of nucleic acids bound to carrier macromolecules
EP2327797B1 (de) 1997-04-01 2015-11-25 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
DE69835516D1 (de) * 1997-05-16 2006-09-21 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
HUP0002356A2 (hu) * 1997-07-22 2000-11-28 Qiagen Genomics, Inc. Nukleinsav-mátrixokon lejátszódó amplifikáció és más enzimatikus reakciók
NZ501776A (en) 1997-07-22 2001-10-26 Qiagen Genomics Inc Spring probe and methods for arraying oligonucleotide and thickening agent to a solid support
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6465178B2 (en) * 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6762019B2 (en) * 1997-09-30 2004-07-13 Surmodics, Inc. Epoxide polymer surfaces
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
EP1021558A1 (de) 1997-10-10 2000-07-26 President And Fellows Of Harvard College Oberflächengebundene, doppelsträngige dna-protein-arrays
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6251660B1 (en) 1997-11-25 2001-06-26 Mosaic Technologies, Inc. Devices and methods for detecting target molecules in biological samples
KR20010040747A (ko) * 1998-02-09 2001-05-15 다나베 히로까즈 디엔에이를 고정화해서 증폭하기 위한 기질, 그 기질에디엔에이를 고정화한 디엔에이고정화칩 및 디엔에이를증폭하는 방법
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Dnaプローブアレー
DE69940647D1 (de) 1998-05-01 2009-05-07 Gen Probe Inc Automatische diagnostische Analysevorrichtung
CA2331767A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
EP1088105A4 (de) * 1998-06-19 2003-06-25 Exact Sciences Corp Detektion nicht-viraler organismen mittels srp-rna
KR100369694B1 (ko) * 1998-09-17 2003-01-29 인터내셔널 비지네스 머신즈 코포레이션 화학적 태그의 합성 방법
WO2000017401A2 (en) 1998-09-21 2000-03-30 Ramot University Authority For Applied Research & Industrial Development Ltd. Method and apparatus for effecting and monitoring nucleic acid amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
AU1042000A (en) * 1998-10-21 2000-05-08 Noxxon Pharma Ag Method for the exponential amplification of molecular matrices
US6458566B2 (en) * 1998-10-23 2002-10-01 Albert Einstein College Of Medicine Of Yeshiva University Method of identification of differentially expressed MRNA
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
AU1817300A (en) * 1998-11-13 2000-06-05 Mosaic Technologies Multielement analytical device for assay of nucleic acid sequences and uses therefore
DE19854946C2 (de) 1998-11-27 2002-01-03 Guenter Von Kiedrowski Klonieren und Kopieren an Oberflächen
AU772213C (en) * 1999-02-26 2004-12-09 Exact Sciences Corporation Biochemical purification devices with immobilized capture probes and their uses
US6326173B1 (en) * 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
JP2002541475A (ja) * 1999-04-12 2002-12-03 ナノゲン/ベクトン・ディッキンソン・パートナーシップ 鎖置換増幅およびバイオエレクトロニック・マイクロチップ技術を用いる核酸配列の増幅および分離
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6221635B1 (en) 1999-05-06 2001-04-24 The Wistar Institute Methods for solid-phase amplification of DNA template (SPADT) using multiarrays
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
JP3911909B2 (ja) 1999-06-09 2007-05-09 株式会社日立製作所 Dna試料調製方法及びdna試料調製装置
EP1110967A1 (de) * 1999-12-21 2001-06-27 LION Bioscience AG Ein Biomolekül und ein Organosilan enthaltende Verbindung
EP1111068A1 (de) * 1999-12-21 2001-06-27 LION Bioscience AG Verzweigte Substanz zur Verwendung in Reaktionen zur Detektion und Analyse von Nukleinsäuren
AU2843201A (en) * 1999-12-21 2001-07-03 Vbc-Genomics Bioscience Research Gmbh Compound comprising a peptide moiety and an organo-silane moiety.
AU3015801A (en) * 1999-12-23 2001-07-09 Axaron Bioscience Ag Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
DE19962893A1 (de) * 1999-12-23 2001-07-12 Basf Lynx Bioscience Ag Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
AU2282801A (en) 1999-12-29 2001-07-09 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
WO2001062982A2 (en) * 2000-02-25 2001-08-30 Mosaic Technologies, Inc. Methods for multi-stage solid phase amplification of nucleic acids
EP1294935A2 (de) * 2000-03-14 2003-03-26 Investigen Zusammensetzungen und methoden zur simultanen detektion unterschiedlicher biologischer spezies
US6376191B1 (en) 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
WO2001075154A2 (de) * 2000-04-03 2001-10-11 Axaron Bioscience Ag Neue verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
DE10049363A1 (de) * 2000-04-20 2001-10-31 Adnagen Gmbh Verfahren, Diagnose-Kit und Mikroarray zur Bestimmung des Rhesusfaktors
KR20010097731A (ko) * 2000-04-25 2001-11-08 김희태 다양한 종류의 특이 핵산 시발자( 이하 프라이머 )를피씨알 튜브안에 함께 부착하여 핵산 중합 반응( 이하피씨알 )을 함께 수행하여 준 뒤 여기에 표지자가 부착된탐침자( 이하 프로브 )를 핵산 결합 반응을 시켜주어유전정보를 알 수 있게 하는 실험기법.
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
DE10040857A1 (de) 2000-08-11 2002-02-28 Jens P Fuerste Nukleinsäurenbibliothek oder Protein- oder Peptidbibliothek
ATE316581T1 (de) 2000-09-05 2006-02-15 Zeltz Patrick Dr Verfahren zur spezifischen bestimmung von dna- sequenzen mittels paralleler amplifikation
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP1427847B1 (de) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
MXPA02012739A (es) * 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
US6869532B2 (en) * 2001-06-04 2005-03-22 Cuno Incorporated Nucleic acid binding matrix
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
AT500427B1 (de) * 2002-05-29 2009-02-15 Anagnostics Bioanalysis Gmbh Vorrichtung zur analyse von bestandteilen einer probe
JP3917640B2 (ja) * 2002-07-26 2007-05-23 株式会社東芝 核酸プローブ固定化基体を用いた標的核酸の存在を検出する方法
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
DE10244456A1 (de) * 2002-09-24 2004-04-15 Hain Lifescience Gmbh Verfahren zum Nachweis und Differenzierung von Bakterien
WO2004031351A2 (en) * 2002-10-01 2004-04-15 Nimblegen Systems, Inc. Microarrays having multiple oligonucleotides in single array features
WO2004067765A2 (en) * 2003-01-29 2004-08-12 Keck Graduate Institute Organism fingerprinting using nicking agents
CN1791682B (zh) * 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7722456B2 (en) * 2003-03-04 2010-05-25 Igt Method and apparatus for associating symbols with a state of a gaming device
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
JP2006523465A (ja) * 2003-04-14 2006-10-19 ニューゲン テクノロジーズ, インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US20050039420A1 (en) * 2003-07-31 2005-02-24 Albritton Charles Wade Fluid sensing in a drip tray
WO2005028629A2 (en) * 2003-09-19 2005-03-31 Applera Corporation Whole genome expression analysis system
EP1706488B1 (de) 2004-01-12 2013-07-24 Roche NimbleGen, Inc. Verfahren zur pcr-amplifikation auf einem microarray
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
US20050260636A1 (en) * 2004-03-24 2005-11-24 Li X J Methods for the detection of biological organisms using transfer ribonucleic acids as biomarkers
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7026851B2 (en) * 2004-05-12 2006-04-11 System General Corp. PWM controller having frequency jitter for power supplies
WO2006073504A2 (en) * 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP2236628A3 (de) 2005-02-01 2010-10-13 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
EP2857523A1 (de) 2005-02-01 2015-04-08 Applied Biosystems, LLC Verfahren zur Sequenzbestimmung in einem Polynucleotid
US20060257907A1 (en) * 2005-04-19 2006-11-16 The Regents Of The University Of California Packed bed for nucleic acid capture and amplification
NZ563759A (en) 2005-05-31 2010-06-25 Mylan Lab Inc Compositions comprising nebivolol, a hydralazine compound and an isosorbide nitrate
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
EP1929046B1 (de) * 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
AT502549B1 (de) * 2005-10-07 2007-06-15 Anagnostics Bioanalysis Gmbh Vorrichtung zur analyse von flüssigen proben
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) * 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US20070148677A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and systems for acquiring real-time quantitative melt data
US20070148639A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and devices for nucleic acid amplification on a surface
US20080076131A1 (en) * 2005-12-02 2008-03-27 Chagovetz Alexander M Methods and devices for nucleic acid amplification on a surface
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
WO2007107710A1 (en) * 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
WO2007111937A1 (en) * 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20090275486A1 (en) * 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
EP2038426A2 (de) * 2006-06-16 2009-03-25 Sequenom, Inc. Verfahren und zusammensetzungen für amplifikation, nachweis und quantifizierung einer nukleinsäure aus einer probe
US9328378B2 (en) * 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US20100240544A1 (en) * 2006-09-29 2010-09-23 Liu David J Aptamer biochip for multiplexed detection of biomolecules
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US12497656B2 (en) 2007-01-26 2025-12-16 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US7835871B2 (en) 2007-01-26 2010-11-16 Illumina, Inc. Nucleic acid sequencing system and method
JP5151167B2 (ja) * 2007-01-31 2013-02-27 住友化学株式会社 Dnaメチル化測定方法
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
EP2140031A4 (de) 2007-03-26 2011-04-20 Sequenom Inc Restriktionsendonukleaseverstärkte polymorphe sequenzerfassung
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
CN103157400B (zh) 2007-06-21 2014-12-24 简·探针公司 用于将容器暴露于多个热区的仪器和方法
JP2009000070A (ja) * 2007-06-22 2009-01-08 Hitachi Ltd 大規模並列核酸分析方法
EP2201021A4 (de) * 2007-08-29 2012-01-25 Applied Biosystems Llc Alternative nukleinsäuresequenzierverfahren
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US20090191553A1 (en) * 2007-10-01 2009-07-30 Applied Biosystems Inc. Chase Ligation Sequencing
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8932879B2 (en) * 2007-11-06 2015-01-13 Ambergen, Inc. Methods and compounds for phototransfer
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US8481263B2 (en) * 2007-11-06 2013-07-09 Ambergen Bead-ligand-nascent protein complexes
US20090270278A1 (en) * 2007-11-06 2009-10-29 Ambergen, Inc. Methods and compounds for making arrays
US20090286286A1 (en) * 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
US8486634B2 (en) 2007-11-06 2013-07-16 Ambergen, Inc. Amplifying bisulfite-treated template
US20090264298A1 (en) * 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
US20100075374A1 (en) * 2007-11-08 2010-03-25 Ambergen, Inc. Methods for capturing nascent proteins
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US8202691B2 (en) * 2008-01-25 2012-06-19 Illumina, Inc. Uniform fragmentation of DNA using binding proteins
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US8999642B2 (en) 2008-03-10 2015-04-07 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US9624489B2 (en) 2008-03-10 2017-04-18 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US7846666B2 (en) * 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
JP2009240249A (ja) * 2008-03-31 2009-10-22 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出用基板
US9518288B2 (en) 2008-04-11 2016-12-13 University Of Utah Research Foundation Methods and compositions related to quantitative, array based methylation analysis
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
EP2607496B1 (de) 2008-12-23 2014-07-16 Illumina, Inc. Für Nukleinsäuresequenzierungsprotokolle geeignete Verfahren
CA2750900C (en) 2009-01-30 2017-03-28 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP2435461B1 (de) 2009-05-29 2017-08-09 Life Technologies Corporation Nukleinsäuregerüst-polymerpartikel und verfahren zu ihrer herstellung und verwendung
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2494353B1 (de) 2009-10-30 2017-09-27 Illumina Inc. Vorrichtung mit einer Mehrzahl von codierten Mikrogefäßen und einer Vielzahl von Fächern und ein Verfahren zum Lesen einer Mehrzahl von codierten Mikrogefäßen
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011071923A2 (en) 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
EP2517025B1 (de) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Verfahren zur reduzierung des austauschs von molekülen zwischen tröpfchen
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4435111A1 (de) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digitale analytanalyse
CN202281746U (zh) 2010-03-06 2012-06-20 伊鲁米那股份有限公司 检测来自样品光信号的测定设备及其光学组件和光学系统
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
KR20130029128A (ko) 2010-07-23 2013-03-21 베크만 컬터, 인코포레이티드 실시간 pcr용 용기
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン9・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
US20140378333A1 (en) * 2011-09-13 2014-12-25 Tufts University Digital bridge pcr
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
AU2012336040B2 (en) 2011-11-07 2015-12-10 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US8973736B2 (en) 2011-11-07 2015-03-10 Beckman Coulter, Inc. Magnetic damping for specimen transport system
CN104040352B (zh) 2011-11-07 2016-09-07 贝克曼考尔特公司 机械臂
EP3373015A1 (de) 2011-11-07 2018-09-12 Beckman Coulter Inc. Aliquotierungssystem und arbeitsablauf
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
WO2013070755A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Centrifuge system and workflow
US9910054B2 (en) 2011-11-07 2018-03-06 Beckman Coulter, Inc. System and method for processing samples
DE102011055247A1 (de) 2011-11-10 2013-05-16 Albert-Ludwigs-Universität Freiburg Multianalyt-Reportersystem
US9200274B2 (en) 2011-12-09 2015-12-01 Illumina, Inc. Expanded radix for polymeric tags
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
NO2694769T3 (de) 2012-03-06 2018-03-03
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
JP6159391B2 (ja) 2012-04-03 2017-07-05 イラミーナ インコーポレーテッド 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
EP2885626B1 (de) 2012-08-20 2020-03-04 Illumina, Inc. Ein integriertes detektions-, durchflusszell- und photonik-gerät (dfp)
US10066264B2 (en) 2012-08-30 2018-09-04 Kabushiki Kaisha Dnaform Method for analyzing target nucleic acid, kit, and analyzer
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN105190656B (zh) 2013-01-17 2018-01-16 佩索纳里斯公司 用于遗传分析的方法和系统
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP2970951B1 (de) 2013-03-13 2019-02-20 Illumina, Inc. Verfahren zur nukleinsäuresequenzierung
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
WO2014160254A2 (en) 2013-03-14 2014-10-02 Abbott Molecular Inc. Minimizing errors using uracil-dna-n-glycosylase
CN105378107A (zh) 2013-03-14 2016-03-02 雅培分子公司 多重甲基化-特异性扩增系统和方法
EP3533884A1 (de) 2013-03-15 2019-09-04 Ibis Biosciences, Inc. Dna-sequenzen zur beurteilung der kontamination in der dna-sequenzierung
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
US9890425B2 (en) 2013-03-15 2018-02-13 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
DK3431614T3 (da) 2013-07-01 2021-12-06 Illumina Inc Katalysator fri overfladefunktionalisering og polymerpodning
CN105358715B (zh) 2013-07-03 2018-09-18 伊鲁米那股份有限公司 正交合成测序
WO2015013465A2 (en) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CN113789372B (zh) 2013-08-08 2025-02-25 伊鲁米那股份有限公司 用于向流动池的试剂输送的流体系统
EP3038834B1 (de) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation von tröpfchen auf hydrophilen oder variegiert hydrophilen oberflächen
GB2534067B (en) 2013-08-30 2021-07-21 Personalis Inc Methods and systems for genomic analysis
GB2535066A (en) 2013-10-03 2016-08-10 Personalis Inc Methods for analyzing genotypes
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ, インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
EP3080268A1 (de) 2013-12-09 2016-10-19 Illumina, Inc. Verfahren und zusammensetzungen zur gezielten nukleinsäuresequenzierung
EP4220137A1 (de) 2013-12-10 2023-08-02 Illumina, Inc. Biosensoren zur biologischen oder chemischen analyse und verfahren zur herstellung davon
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP3084523B1 (de) 2013-12-19 2019-07-03 Illumina, Inc. Substrate mit nanostrukturierungsoberflächen und verfahren zur herstellung davon
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2015259024B2 (en) 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
JP6960223B2 (ja) 2014-05-27 2021-11-05 イラミーナ インコーポレーテッド 基礎器具と取外可能カートリッジとを含む生化学的解析のためのシステム及び方法
RU2688746C2 (ru) 2014-06-05 2019-05-22 Иллумина, Инк. Системы и способы с использованием поворотного клапана для по меньшей мере одного из приготовления образца или анализа образца
SG11201610357UA (en) 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
EP3169799B1 (de) 2014-07-15 2019-09-04 Qiagen Sciences, LLC Halbzufällige strichcodes zur nukleinsäureanalyse
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
KR102538753B1 (ko) 2014-09-18 2023-05-31 일루미나, 인코포레이티드 핵산 서열결정 데이터를 분석하기 위한 방법 및 시스템
MX391361B (es) 2014-10-09 2025-03-21 Illumina Inc Metodo y dispositivo para la separacion de liquidos inmiscibles para aislar de modo efectivo al menos uno de los liquidos.
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
PT3212684T (pt) 2014-10-31 2020-02-03 Illumina Cambridge Ltd Revestimentos de polímeros e de copolímero de dna
WO2016077426A1 (en) 2014-11-11 2016-05-19 Abbott Molecular Inc. Hybridization probes and methods
EP3247804B1 (de) 2015-01-23 2020-08-05 Qiagen Sciences, LLC Hochmultiplex-pcr mit molekularer strichcodierung
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2979850C (en) 2015-03-24 2020-07-21 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
EP4321627A3 (de) 2015-04-10 2024-04-17 10x Genomics Sweden AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
US11220705B2 (en) 2015-04-24 2022-01-11 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
CN107810415B (zh) 2015-05-11 2021-06-22 亿明达股份有限公司 用于发现和分析治疗剂的平台
KR102134953B1 (ko) 2015-05-29 2020-07-16 일루미나, 인코포레이티드 지정된 반응을 수행하기 위한 샘플 캐리어 및 검정 시스템
HK1253950A1 (zh) 2015-07-07 2019-07-05 Illumina, Inc. 经由纳米压印的选择性表面图案化
EP3325648B1 (de) 2015-07-17 2023-03-29 Illumina, Inc. Polymerfolien zur sequenzierung von anwendungen
IL294145B2 (en) 2015-07-30 2024-02-01 Illumina Inc Removal of orthogonal blocking of nucleotides
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
WO2017037078A1 (en) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
JP2019508669A (ja) 2016-01-11 2019-03-28 イラミーナ インコーポレーテッド マイクロフルオロメータ、流体システム、およびフローセルラッチクランプモジュールを有する検出装置
EP3433379B1 (de) 2016-03-25 2021-12-29 Qiagen Sciences, LLC Primer mit einander ergänzenden sequenzen zur mehrfachen strangverdrängungsamplifizierung
SG11201805665UA (en) 2016-03-28 2018-10-30 Illumina Inc Multi-plane microarrays
CN109313396B (zh) 2016-05-18 2022-11-22 Illumina公司 使用图案化疏水性表面进行的自组装图案化
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
PT3464628T (pt) 2016-06-06 2024-06-03 Redvault Biosciences Lp Construções repórter de alvo e suas utilizações
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
EP4180539A1 (de) 2016-07-12 2023-05-17 Qiagen Sciences, LLC Einzelende-duplex-dna-sequenzierung
EP3488017A4 (de) 2016-07-20 2020-02-26 Genapsys Inc. Systeme und verfahren zur nukleinsäuresequenzierung
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A3 (de) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nukleinsäurebasierte datenspeicherung
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
CN111781139B (zh) 2016-10-14 2023-09-12 亿明达股份有限公司 夹盒组件
DE102016120124B8 (de) 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
CA3044231A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
EP3929306A1 (de) 2017-01-26 2021-12-29 QIAGEN GmbH Verfahren zur anreicherung von schablonennukleinsäuren
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2019023951A1 (zh) 2017-08-01 2019-02-07 深圳华大智造科技有限公司 核酸测序方法
JP2021500531A (ja) 2017-08-15 2021-01-07 オムニオム インコーポレイテッドOmniome, Inc. 化学的および生物学的検体の検出に有用なスキャン装置および方法
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
EP3696275B1 (de) 2017-10-11 2025-12-24 MGI Tech Co., Ltd. Verfahren zur verbesserung der belastung und stabilität von nukleinsäuren auf einem festen träger
EP3622519B1 (de) 2017-10-16 2023-09-13 Illumina, Inc. Auf tiefenlernen basierende detektion von aberranter spleissung
US11315016B2 (en) 2017-10-16 2022-04-26 Illumina, Inc. Deep convolutional neural networks for variant classification
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019084158A1 (en) 2017-10-26 2019-05-02 Ultima Genomics, Inc. METHODS AND SYSTEMS FOR SEQUENCE CALL
US11499962B2 (en) 2017-11-17 2022-11-15 Ultima Genomics, Inc. Methods and systems for analyte detection and analysis
US10267790B1 (en) 2017-11-17 2019-04-23 Ultima Genomics, Inc. Systems for biological sample processing and analysis
IL312616B2 (en) 2018-01-04 2025-08-01 Twist Bioscience Corp Dna-based digital information storage
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
KR102370055B1 (ko) 2018-01-08 2022-03-03 일루미나, 인코포레이티드 반도체-기반 검출을 사용하는 고-처리율 서열분석용 시스템 및 디바이스
WO2019140402A1 (en) 2018-01-15 2019-07-18 Illumina, Inc. Deep learning-based variant classifier
AU2019240046B2 (en) 2018-03-22 2022-04-14 Illumina, Inc. Preparation of nucleic acid libraries from RNA and DNA
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
WO2019200338A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant classifier based on deep neural networks
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP7554117B2 (ja) 2018-04-26 2024-09-19 パシフィック・バイオサイエンシズ・オブ・カリフォルニア・インク. 核酸-ヌクレオチド-ポリメラーゼ複合体を安定化するための方法及び組成物
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
JP2021525078A (ja) 2018-05-31 2021-09-24 オムニオム インコーポレイテッドOmniome, Inc. 核酸配列決定におけるシグナル対ノイズの増加
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
CN113767175B (zh) 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113705585A (zh) 2018-10-15 2021-11-26 因美纳有限公司 基于神经网络实现的方法和系统
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
CN211311438U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 流动池
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US12239980B2 (en) 2018-12-07 2025-03-04 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
US10512911B1 (en) 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894585A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von erfassungssonden zur räumlichen analyse
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
EP3899032A2 (de) 2018-12-20 2021-10-27 Omniome, Inc. Temperaturkontrolle zur analyse von nukleinsäuren und anderen analyten
EP3902816A4 (de) 2018-12-26 2022-09-14 Twist Bioscience Corporation Hochgenaue de-novo-polynukleotidsynthese
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN119662792A (zh) 2019-02-19 2025-03-21 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
US11680950B2 (en) 2019-02-20 2023-06-20 Pacific Biosciences Of California, Inc. Scanning apparatus and methods for detecting chemical and biological analytes
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
WO2020185790A1 (en) 2019-03-10 2020-09-17 Ultima Genomics, Inc. Methods and systems for sequence calling
US10830703B1 (en) 2019-03-14 2020-11-10 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
US11118223B2 (en) 2019-03-14 2021-09-14 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
KR102901606B1 (ko) 2019-03-14 2025-12-18 울티마 제노믹스, 인크. 분석물 검출 및 분석을 위한 방법, 장치, 및 시스템
US10900078B2 (en) 2019-03-14 2021-01-26 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
US10852518B1 (en) 2019-03-14 2020-12-01 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
WO2020205296A1 (en) 2019-03-21 2020-10-08 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
CA3104851A1 (en) 2019-05-16 2020-11-19 Illumina, Inc. Base calling using convolutions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (de) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible nukleotide änderung
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
JP7470787B2 (ja) 2019-11-05 2024-04-18 パーソナリス,インコーポレイティド 単一試料からの腫瘍純度の推定
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
AU2020400030B2 (en) 2019-12-09 2026-05-21 Twist Bioscience Corporation Variant nucleic acid libraries for adenosine receptors
EP4752521A3 (de) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zur verwendung davon
US11747262B2 (en) 2019-12-23 2023-09-05 Singular Genomics Systems, Inc. Flow cell carrier and methods of use
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US11498078B2 (en) 2019-12-23 2022-11-15 Singular Genomics Systems, Inc. Flow cell receiver and methods of use
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
CN115667507A (zh) 2019-12-31 2023-01-31 奇异基因组学系统公司 用于长读出测序的多核苷酸条形码
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
US12591780B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Data compression for artificial intelligence-based base calling
US12592298B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Hardware execution and acceleration of artificial intelligence-based base caller
JP7747643B2 (ja) 2020-02-20 2025-10-01 イルミナ インコーポレイテッド 人工知能ベースの多対多ベースコール
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
CN115836135A (zh) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 用于修饰聚合酶-核酸复合物的组合物和方法
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
WO2021231263A2 (en) 2020-05-12 2021-11-18 Singular Genomics Systems, Inc. Nucleic acid amplification methods
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153775B1 (de) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
EP4600376A3 (de) 2020-06-02 2025-10-22 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
EP4164796A4 (de) 2020-06-10 2024-03-06 10x Genomics, Inc. Flüssigkeitsabgabeverfahren
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4450639B1 (de) 2020-06-25 2025-10-15 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
EP4153606A4 (de) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. Verfahren zur sequenzierung komplementärer polynukleotide
KR20230041808A (ko) 2020-07-29 2023-03-24 엠쥐아이 테크 컴퍼니 엘티디. 고체 지지체에 핵산 분자를 로딩하는 방법
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022072652A1 (en) 2020-09-30 2022-04-07 Ultima Genomics, Inc. Methods, systems, and apparatus for high throughput sequencing
EP4208470A4 (de) 2020-10-22 2024-10-30 Singular Genomics Systems, Inc. Nukleinsäurezirkularisierung und amplifikation auf einer oberfläche
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12576399B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for imaging and analyzing a cell in a compartment of a fluidic device
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
WO2022170212A1 (en) 2021-02-08 2022-08-11 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
US20220301657A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
EP4524261B1 (de) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4314324A4 (de) 2021-03-30 2025-03-05 Ultima Genomics, Inc. Gutartige, narbenbildende spaltbare linker
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
WO2022232308A1 (en) * 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12570831B2 (en) 2021-06-15 2026-03-10 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
WO2023278184A1 (en) * 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
CN117546249B (zh) 2021-06-29 2026-08-04 因美纳有限公司 使用寡核苷酸序列训练的自学碱基检出器
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
EP4679300A3 (de) 2021-07-19 2026-04-22 Illumina, Inc. Intensitätsextraktion mit interpolation und anpassung für basisanrufe
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
EP4373958A1 (de) 2021-07-23 2024-05-29 Illumina, Inc. Verfahren zur herstellung einer substratoberfläche zur dna-sequenzierung
KR20240037882A (ko) 2021-07-28 2024-03-22 일루미나, 인코포레이티드 염기 호출 시스템의 품질 점수 보정
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023014741A1 (en) 2021-08-03 2023-02-09 Illumina Software, Inc. Base calling using multiple base caller models
WO2023023357A2 (en) 2021-08-20 2023-02-23 Ultima Genomics, Inc. Systems and methods for sample preparation for sequencing
EP4388134A4 (de) 2021-08-20 2025-10-29 Singular Genomics Systems Inc Chemische und thermische unterstützte nukleinsäureamplifikationsverfahren
EP4344443A1 (de) 2021-08-31 2024-04-03 Illumina, Inc. Durchflusszelle mit verbesserter bohrlochabbildungsauflösung
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4395933A1 (de) 2021-09-03 2024-07-10 Elegen Corporation Vorrichtungen, systeme und verfahren zur mehrweg-wulstsortierung unter verwendung von druckquellen
WO2023059654A1 (en) 2021-10-05 2023-04-13 Personalis, Inc. Customized assays for personalized cancer monitoring
CN118076747A (zh) 2021-10-11 2024-05-24 深圳华大智造科技股份有限公司 皂苷类化合物在核酸测序中的用途
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023076833A1 (en) 2021-10-26 2023-05-04 Singular Genomics Systems, Inc. Multiplexed targeted amplification of polynucleotides
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
US20230183799A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
JP2025509651A (ja) 2022-03-15 2025-04-11 イルミナ インコーポレイテッド 並行サンプル及びインデックス配列決定
US12661647B2 (en) 2022-03-22 2026-06-23 Cellanome, Inc. Methods for enclosing and analyzing a cell in a fluidic device
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
US20240052406A1 (en) * 2022-08-04 2024-02-15 Singular Genomics Systems, Inc. Competitive methods and compositions for amplifying polynucleotides
US20260071264A1 (en) 2022-09-09 2026-03-12 Bgi Shenzhen Use of heteroaromatic ring compounds in nucleic acid detection
EP4587588A1 (de) 2022-09-16 2025-07-23 Illumina, Inc. Nanopartikel mit polynukleotidbindungsstelle und verfahren zur herstellung davon
EP4685239A1 (de) 2022-11-09 2026-01-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates
CN121241055A (zh) 2023-05-19 2025-12-30 深圳华大智造科技股份有限公司 一种荧光染料、其合成及其应用
WO2024243873A1 (zh) 2023-05-31 2024-12-05 深圳华大智造科技股份有限公司 一种调整核酸纳米球体积的方法及其应用
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2026006629A1 (en) 2024-06-27 2026-01-02 Personalis, Inc. System and method for management of compressed sequencing files
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
WO1990002205A1 (en) * 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2004326A1 (en) * 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
IE66572B1 (en) * 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
KR920700360A (ko) * 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
GB2233654A (en) * 1989-07-07 1991-01-16 Nat Res Dev Immobilised polynucleotides
GB9019512D0 (en) * 1990-09-06 1990-10-24 Ici Plc Assay method
CA2055755A1 (en) * 1990-11-22 1992-05-23 Toshihiko Kishimoto Method of immobilizing single-stranded dna on carrier at terminal
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
WO1993009250A1 (en) * 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes

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EP0784701A1 (de) 1997-07-23
DE69526511D1 (de) 2002-05-29
EP0784701B1 (de) 2002-04-24
WO1996004404A1 (en) 1996-02-15
CA2196604A1 (en) 1996-02-15
JPH10505492A (ja) 1998-06-02
US5641658A (en) 1997-06-24

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