ATE230032T1 - Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund - Google Patents

Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund

Info

Publication number
ATE230032T1
ATE230032T1 AT96937697T AT96937697T ATE230032T1 AT E230032 T1 ATE230032 T1 AT E230032T1 AT 96937697 T AT96937697 T AT 96937697T AT 96937697 T AT96937697 T AT 96937697T AT E230032 T1 ATE230032 T1 AT E230032T1
Authority
AT
Austria
Prior art keywords
solid supports
specific background
hybridization
producing solid
background reduction
Prior art date
Application number
AT96937697T
Other languages
English (en)
Inventor
Ness Jeffrey Van
Original Assignee
Qiagen Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Genomics Inc filed Critical Qiagen Genomics Inc
Application granted granted Critical
Publication of ATE230032T1 publication Critical patent/ATE230032T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Manufacture And Refinement Of Metals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT96937697T 1995-10-18 1996-10-17 Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund ATE230032T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US650195P 1995-10-18 1995-10-18
PCT/US1996/016777 WO1997014815A1 (en) 1995-10-18 1996-10-17 Methods for preparing solid supports for hybridization and reducing non-specific background

Publications (1)

Publication Number Publication Date
ATE230032T1 true ATE230032T1 (de) 2003-01-15

Family

ID=21721191

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96937697T ATE230032T1 (de) 1995-10-18 1996-10-17 Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund

Country Status (9)

Country Link
US (1) US5994065A (de)
EP (1) EP0862655B1 (de)
JP (1) JPH11514872A (de)
KR (1) KR19990064358A (de)
AT (1) ATE230032T1 (de)
AU (1) AU723889B2 (de)
CA (1) CA2235671A1 (de)
DE (1) DE69625510T2 (de)
WO (1) WO1997014815A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0963443B1 (de) 1996-12-10 2006-03-08 Sequenom, Inc. Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
US7173011B2 (en) 2000-11-20 2007-02-06 University Of Southern California Radiation therapy methods
JP2002512045A (ja) * 1998-04-20 2002-04-23 アフィメトリックス インコーポレイテッド 核酸プローブアレイに対する非特異的な結合を減少させるための方法
CA2348054C (en) * 1998-10-23 2012-06-19 Qiagen Gesellschaft Mit Beschrankter Haftung Processes and means for the isolation and purification of nucleic acids at surfaces
AU6052001A (en) 2000-03-29 2001-10-08 Ct For The Applic Of Molecular Methods for genotyping by hybridization analysis
DE10027120A1 (de) * 2000-05-23 2001-12-06 Epigenomics Ag Probenträger für Massenspektrometer
WO2002040962A2 (en) * 2000-11-15 2002-05-23 Benchmark Science, Inc. Methods and reagents to reduce backgrounds of heterogeneous assays
JP3523188B2 (ja) * 2000-12-13 2004-04-26 富士写真フイルム株式会社 プローブ分子が固定された検出具の水性処理液
DE10130800B4 (de) 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
JP2003084002A (ja) 2001-06-27 2003-03-19 Fuji Photo Film Co Ltd 蛍光検出におけるバックグラウンドの低減方法
US20030082969A1 (en) * 2001-10-25 2003-05-01 Trevor Arthurs Breathable barrier extruded laminate
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
GB2392977A (en) * 2002-09-13 2004-03-17 Suisse Electronique Microtech A fluidic dielectrophoretic system and method for analysing biomolecules
AU2002952702A0 (en) * 2002-11-18 2002-11-28 Murdoch Childrens Research Institute A diagnostic assay
US7368082B1 (en) * 2002-12-12 2008-05-06 Tung-Lian Huang Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles
ATE551433T1 (de) 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
US20060257958A1 (en) * 2005-05-13 2006-11-16 Pronucleotein Biotechnologies, Llc Magnetically-assisted test strip cartridge and method for using same
US20090246754A1 (en) * 2008-02-19 2009-10-01 Intelligent Mdx Optimized probes and primers and methods of using same for the detection and quantitation of bk virus
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090258342A1 (en) * 2008-04-09 2009-10-15 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the detection, quantification and grouping of hiv-1
US20110200995A1 (en) 2009-09-04 2011-08-18 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci
US8877909B2 (en) 2011-04-04 2014-11-04 Intelligent Medical Devices, Inc. Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus
WO2014066749A2 (en) 2012-10-25 2014-05-01 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the binding, detection, differentiation, isolation and sequencing of influenza a; influenza b and respiratory syncytial virus
WO2014137906A1 (en) 2013-03-05 2014-09-12 Intelligent Medical Devices, Inc. Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
WO1992003579A1 (en) * 1990-08-16 1992-03-05 Diagnostic Biotechnology, Inc. An augmented western blot format and immunoassay for detection of viral antibodies
WO1994000600A1 (en) * 1992-06-25 1994-01-06 Microprobe Corporation Solid supports for nucleic acid hybridization assays

Also Published As

Publication number Publication date
JPH11514872A (ja) 1999-12-21
KR19990064358A (ko) 1999-07-26
US5994065A (en) 1999-11-30
WO1997014815A1 (en) 1997-04-24
EP0862655A1 (de) 1998-09-09
AU7517496A (en) 1997-05-07
EP0862655B1 (de) 2002-12-18
DE69625510T2 (de) 2003-07-31
DE69625510D1 (de) 2003-01-30
AU723889B2 (en) 2000-09-07
CA2235671A1 (en) 1997-04-24

Similar Documents

Publication Publication Date Title
ATE230032T1 (de) Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund
DE69729122D1 (de) KüNSTLICHE FEHLHYBRIDISIERUNG
DE69607830D1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
ATE164883T1 (de) Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen
ATE211769T1 (de) Oligonukleotide und verfahren zum nachweis von chlamydia trachomatis
ATE141956T1 (de) Verfahren zur vervielfältigung von nukleinsäuresequenzen
DE69509925D1 (de) Verfahren und vorrichtung zur herstellung von mikromatrizen für biologische proben
ATE374833T1 (de) Verfahren zur synthese von nukleinsäuren
DE69627314D1 (de) Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung
DE59310281D1 (de) Verfahren zur präparierung und hybridisierung von spezifischen proben
ATE244506T1 (de) Oligonukleotidmodulation der expression von multiwirkstoff-resistenz -assoziiertem protein
ATE250671T1 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
DE68925717D1 (de) Verfahren und Reagenzkombination zur Bestimmung von Nukleotidsequenzen
WO1997014815B1 (en) Methods for preparing solid supports for hybridization and reducing non-specific background
Ruf-Zamojski et al. Regulatory architecture of the LβT2 gonadotrope cell underlying the response to gonadotropin-releasing hormone
ATE401424T1 (de) Verfahren zur identifizierung von nukleinsäureliganden für gewebsziele
DE69534217D1 (de) Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen
ATE465261T1 (de) Integrin-gekoppelte kinase, ihre hemmstoffe und deren gebrauch für verfahren zur therapeutischen behandlung; gentherapie und bereitstellung von hemmstoffen, die als pseudosubstrate wirken
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
DE69941690D1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
DE69924128D1 (de) Verfahren zur erkennung von verbindungen,die die wechselwirkung von rna und rna-bindungsproteinen beinflussen
ATE321308T1 (de) Verfahren zur auswahl von oligonukleotiden mit niedriger kreuzhybridisierung
DE69636725D1 (de) Verfahren zur trennung und/oder erkennung von dns-molekülen
DE60035416D1 (de) Ein polynucleotidmotor, ein motorsystem, seine herstellung und verwendungen
ATE105871T1 (de) Verfahren zur stabilisierung der hybridisierung von komplementären polynukleotidsequenzen.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties