ATE230032T1 - Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund - Google Patents
Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrundInfo
- Publication number
- ATE230032T1 ATE230032T1 AT96937697T AT96937697T ATE230032T1 AT E230032 T1 ATE230032 T1 AT E230032T1 AT 96937697 T AT96937697 T AT 96937697T AT 96937697 T AT96937697 T AT 96937697T AT E230032 T1 ATE230032 T1 AT E230032T1
- Authority
- AT
- Austria
- Prior art keywords
- solid supports
- specific background
- hybridization
- producing solid
- background reduction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Manufacture And Refinement Of Metals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US650195P | 1995-10-18 | 1995-10-18 | |
| PCT/US1996/016777 WO1997014815A1 (en) | 1995-10-18 | 1996-10-17 | Methods for preparing solid supports for hybridization and reducing non-specific background |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE230032T1 true ATE230032T1 (de) | 2003-01-15 |
Family
ID=21721191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96937697T ATE230032T1 (de) | 1995-10-18 | 1996-10-17 | Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5994065A (de) |
| EP (1) | EP0862655B1 (de) |
| JP (1) | JPH11514872A (de) |
| KR (1) | KR19990064358A (de) |
| AT (1) | ATE230032T1 (de) |
| AU (1) | AU723889B2 (de) |
| CA (1) | CA2235671A1 (de) |
| DE (1) | DE69625510T2 (de) |
| WO (1) | WO1997014815A1 (de) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2274587A1 (en) | 1996-12-10 | 1998-06-18 | Genetrace Systems Inc. | Releasable nonvolatile mass-label molecules |
| US7173011B2 (en) | 2000-11-20 | 2007-02-06 | University Of Southern California | Radiation therapy methods |
| EP1071821A4 (de) * | 1998-04-20 | 2005-06-29 | Affymetrix Inc | Verfahren zur redultion nichtspezifischer bindung an einen nukleinsäuresondenarray |
| DK1121460T3 (da) * | 1998-10-23 | 2010-09-20 | Qiagen Ges Mit Beschraenkter H | Fremgangsmåde og middel til isolering og oprensning af nucleinsyrer på overflader |
| US6713258B2 (en) | 2000-03-29 | 2004-03-30 | Center For The Application Of Molecular Biology To International Agriculture (Cambia) | Methods for genotyping by hybridization analysis |
| DE10027120A1 (de) * | 2000-05-23 | 2001-12-06 | Epigenomics Ag | Probenträger für Massenspektrometer |
| WO2002040962A2 (en) * | 2000-11-15 | 2002-05-23 | Benchmark Science, Inc. | Methods and reagents to reduce backgrounds of heterogeneous assays |
| JP3523188B2 (ja) * | 2000-12-13 | 2004-04-26 | 富士写真フイルム株式会社 | プローブ分子が固定された検出具の水性処理液 |
| DE10130800B4 (de) | 2001-06-22 | 2005-06-23 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität |
| JP2003084002A (ja) | 2001-06-27 | 2003-03-19 | Fuji Photo Film Co Ltd | 蛍光検出におけるバックグラウンドの低減方法 |
| US20030082969A1 (en) * | 2001-10-25 | 2003-05-01 | Trevor Arthurs | Breathable barrier extruded laminate |
| DE10154317B4 (de) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| GB2392977A (en) * | 2002-09-13 | 2004-03-17 | Suisse Electronique Microtech | A fluidic dielectrophoretic system and method for analysing biomolecules |
| AU2002952702A0 (en) * | 2002-11-18 | 2002-11-28 | Murdoch Childrens Research Institute | A diagnostic assay |
| US7368082B1 (en) * | 2002-12-12 | 2008-05-06 | Tung-Lian Huang | Formulation of spotting solution to achieve uniform spot size and morphology and for nondestructive quality control of assay articles |
| CA2584230C (en) | 2005-05-06 | 2012-11-27 | Gen-Probe Incorporated | Methods of nucleic acid target capture |
| US20060257958A1 (en) * | 2005-05-13 | 2006-11-16 | Pronucleotein Biotechnologies, Llc | Magnetically-assisted test strip cartridge and method for using same |
| US20090246754A1 (en) * | 2008-02-19 | 2009-10-01 | Intelligent Mdx | Optimized probes and primers and methods of using same for the detection and quantitation of bk virus |
| US20090215050A1 (en) * | 2008-02-22 | 2009-08-27 | Robert Delmar Jenison | Systems and methods for point-of-care amplification and detection of polynucleotides |
| WO2009126517A2 (en) * | 2008-04-09 | 2009-10-15 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, quantification and grouping of hiv-1 |
| US20110200995A1 (en) | 2009-09-04 | 2011-08-18 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of vancomycin resistance genes and vancomycin resistant enterococci |
| US8877909B2 (en) | 2011-04-04 | 2014-11-04 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantitation, monitoring, screening, and sequencing of group B Streptococcus |
| US20140127674A1 (en) | 2012-10-25 | 2014-05-08 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the binding, detection, differentiation, isolation and sequencing of influenza a; influenza b and respiratory syncytial virus |
| US20140256582A1 (en) | 2013-03-05 | 2014-09-11 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
| DE69130396T2 (de) * | 1990-08-16 | 1999-06-24 | Genelabs Diagnostics Pte Ltd., Singapore | Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern |
| WO1994000600A1 (en) * | 1992-06-25 | 1994-01-06 | Microprobe Corporation | Solid supports for nucleic acid hybridization assays |
-
1996
- 1996-10-17 US US08/733,671 patent/US5994065A/en not_active Expired - Lifetime
- 1996-10-17 DE DE69625510T patent/DE69625510T2/de not_active Expired - Fee Related
- 1996-10-17 WO PCT/US1996/016777 patent/WO1997014815A1/en not_active Ceased
- 1996-10-17 KR KR1019980708439A patent/KR19990064358A/ko not_active Withdrawn
- 1996-10-17 AT AT96937697T patent/ATE230032T1/de not_active IP Right Cessation
- 1996-10-17 JP JP9516062A patent/JPH11514872A/ja active Pending
- 1996-10-17 CA CA002235671A patent/CA2235671A1/en not_active Abandoned
- 1996-10-17 AU AU75174/96A patent/AU723889B2/en not_active Ceased
- 1996-10-17 EP EP96937697A patent/EP0862655B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| KR19990064358A (ko) | 1999-07-26 |
| WO1997014815A1 (en) | 1997-04-24 |
| US5994065A (en) | 1999-11-30 |
| DE69625510T2 (de) | 2003-07-31 |
| DE69625510D1 (de) | 2003-01-30 |
| EP0862655B1 (de) | 2002-12-18 |
| JPH11514872A (ja) | 1999-12-21 |
| EP0862655A1 (de) | 1998-09-09 |
| AU723889B2 (en) | 2000-09-07 |
| AU7517496A (en) | 1997-05-07 |
| CA2235671A1 (en) | 1997-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69625510D1 (de) | Verfahren zur herstellung von festen trägern für hybridisierung und zur verminderung von nichtspezifischem hintergrund | |
| DE69729122D1 (de) | KüNSTLICHE FEHLHYBRIDISIERUNG | |
| ATE191934T1 (de) | Verfahren zur isothermer amplifikation von nukleinsäure molekülen | |
| DE69225074D1 (de) | Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen | |
| ATE211769T1 (de) | Oligonukleotide und verfahren zum nachweis von chlamydia trachomatis | |
| ATE141956T1 (de) | Verfahren zur vervielfältigung von nukleinsäuresequenzen | |
| ATE180570T1 (de) | Verfahren und vorrichtung zur herstellung von mikromatrizen für biologische proben | |
| ATE374833T1 (de) | Verfahren zur synthese von nukleinsäuren | |
| FI953445A0 (fi) | Menetelmä nukleiinihappojen herkkään osoitukseen | |
| DE69627314D1 (de) | Kettenabbruchgruppen, ihre verwendung zur sequenzierung und synthese von nukleinsäuren und verfahren zu ihrer herstellung | |
| DE59310281D1 (de) | Verfahren zur präparierung und hybridisierung von spezifischen proben | |
| ATE244506T1 (de) | Oligonukleotidmodulation der expression von multiwirkstoff-resistenz -assoziiertem protein | |
| ATE250671T1 (de) | Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren | |
| DE68925717D1 (de) | Verfahren und Reagenzkombination zur Bestimmung von Nukleotidsequenzen | |
| WO1997014815B1 (en) | Methods for preparing solid supports for hybridization and reducing non-specific background | |
| Ruf-Zamojski et al. | Regulatory architecture of the LβT2 gonadotrope cell underlying the response to gonadotropin-releasing hormone | |
| ATE401424T1 (de) | Verfahren zur identifizierung von nukleinsäureliganden für gewebsziele | |
| DE69534217D1 (de) | Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen | |
| DE69715184D1 (de) | Verfahren zur herstellung von hydroxyaldehyden | |
| ATE465261T1 (de) | Integrin-gekoppelte kinase, ihre hemmstoffe und deren gebrauch für verfahren zur therapeutischen behandlung; gentherapie und bereitstellung von hemmstoffen, die als pseudosubstrate wirken | |
| DE69815370D1 (de) | Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen | |
| DE69941690D1 (de) | Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen | |
| DE69924128D1 (de) | Verfahren zur erkennung von verbindungen,die die wechselwirkung von rna und rna-bindungsproteinen beinflussen | |
| ATE321308T1 (de) | Verfahren zur auswahl von oligonukleotiden mit niedriger kreuzhybridisierung | |
| ATE346165T1 (de) | Verfahren zur trennung und/oder erkennung von dns-molekülen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |