ATE236257T1 - Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet. - Google Patents

Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet.

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Publication number
ATE236257T1
ATE236257T1 AT93915827T AT93915827T ATE236257T1 AT E236257 T1 ATE236257 T1 AT E236257T1 AT 93915827 T AT93915827 T AT 93915827T AT 93915827 T AT93915827 T AT 93915827T AT E236257 T1 ATE236257 T1 AT E236257T1
Authority
AT
Austria
Prior art keywords
cell wall
pct
enzyme
cell
protein
Prior art date
Application number
AT93915827T
Other languages
English (en)
Inventor
Franciscus Maria Klis
Maarten Pleun Schreuder
Holger York Toschka
Cornelis Theodorus Verrips
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of ATE236257T1 publication Critical patent/ATE236257T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT93915827T 1992-07-08 1993-07-07 Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet. ATE236257T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92202080 1992-07-08
EP92203899 1992-12-14
PCT/EP1993/001763 WO1994001567A1 (en) 1992-07-08 1993-07-07 Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein

Publications (1)

Publication Number Publication Date
ATE236257T1 true ATE236257T1 (de) 2003-04-15

Family

ID=26131543

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93915827T ATE236257T1 (de) 1992-07-08 1993-07-07 Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet.

Country Status (16)

Country Link
US (1) US6027910A (de)
EP (1) EP0673427B1 (de)
JP (2) JP3681385B2 (de)
KR (1) KR950702634A (de)
AT (1) ATE236257T1 (de)
AU (1) AU685057B2 (de)
CA (1) CA2139670C (de)
DE (1) DE69332829T2 (de)
DK (1) DK0673427T3 (de)
ES (1) ES2196009T3 (de)
FI (1) FI950042L (de)
NZ (1) NZ254108A (de)
OA (1) OA10123A (de)
PT (1) PT673427E (de)
RU (1) RU95105899A (de)
WO (1) WO1994001567A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
WO1994018330A1 (en) * 1993-02-10 1994-08-18 Unilever N.V. Immobilized proteins with specific binding capacities and their use in processes and products
CN1070533C (zh) * 1993-02-26 2001-09-05 三宝乐啤酒株式会社 酵母凝集基因及含这些基因的酵母
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US6696251B1 (en) * 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
KR100819187B1 (ko) 1999-03-25 2008-04-04 발티온 테크닐리넨 투트키무스케스쿠스 단백질 분배 방법
JP2003284556A (ja) * 1999-11-19 2003-10-07 Toyota Motor Corp 金属捕捉能タンパク質を発現する遺伝子
EP1134231B1 (de) 2000-03-14 2009-04-15 Unilever N.V. Variabele Domänen der schweren Kette eines Antikörpers gegen menschliche Ernährungslipasen und deren Verwendungen
DE10016279A1 (de) * 2000-04-03 2001-10-04 Henkel Kgaa Enzymatisches Färbemittel
ATE403717T1 (de) * 2000-05-01 2008-08-15 Daiichi Sankyo Co Ltd Verfahren zum screening von auf zellwände wirkenden arzneistoffen
AU2001256356A1 (en) * 2000-05-15 2001-11-26 Drochon, Bruno Permeable cements
CN1249227C (zh) 2000-07-07 2006-04-05 卫材株式会社 真菌细胞壁合成基因
AU2002220275A1 (en) * 2000-12-08 2002-06-18 The Board Of Trustees Of The University Of Illinois Mutated class ii major histocompatibility proteins
KR100714670B1 (ko) * 2001-04-19 2007-05-07 간사이가가쿠기카이세이사쿠가부시키가이샤 세포 표층 결합 단백질 및 이의 용도
CN100513548C (zh) * 2001-06-11 2009-07-15 应用超微系统股份有限公司 将AcmA型蛋白质锚融合体与微生物细胞壁材料进行结合的改良方法
DE60232672D1 (de) 2001-10-01 2009-07-30 Dyax Corp Mehrkettige eukaryontische display-vektoren und deren verwendungen
DE60221883T2 (de) * 2001-10-22 2008-04-30 Daiichi Sankyo Co., Ltd. Screeningverfahren für einen auf die zellwand wirkenden arzneistoff
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
EP2497478A3 (de) 2003-12-05 2012-11-07 Children's Hospital Medical Center Oligosaccarid-Zusammensetzungen und ihre Verwendung bei der Behandlung von Infektionen
EP1899477A4 (de) * 2005-07-01 2010-01-20 Medimmune Inc Integrierter ansatz zur erzeugung von heilmitteln mit multidomänen-proteinen
JP2009501522A (ja) * 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
JP2009511067A (ja) * 2005-10-14 2009-03-19 メディミューン，エルエルシー 抗体ライブラリーの細胞提示
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20080138663A1 (en) * 2006-12-12 2008-06-12 Canon Kabushiki Kaisha Microbial electrode and fuel cell and sensor using the same
US20110076752A1 (en) * 2007-02-09 2011-03-31 Medimmune, Llc Antibody library display by yeast cell plasma membrane
EP2137308B1 (de) 2007-03-26 2016-08-03 Agenus Inc. Zelloberflächenpräsentation, screening und produktion von interessierenden proteinen
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
JP5933894B2 (ja) 2007-09-14 2016-06-15 アディマブ， エルエルシー 合理的に設計された、合成抗体ライブラリおよびその使用
US20110275535A1 (en) 2008-12-16 2011-11-10 Novartis Ag Yeast Display Systems
KR102810907B1 (ko) 2010-07-16 2025-05-21 아디맵 엘엘씨 항체 라이브러리
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
EP4284905A1 (de) 2021-01-28 2023-12-06 Novozymes A/S Lipase mit geringer geruchsbildung
JP2025513274A (ja) 2022-04-20 2025-04-24 ノボザイムス アクティーゼルスカブ 遊離脂肪酸の生成法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU626288B2 (en) * 1988-02-05 1992-07-30 Commonwealth Scientific And Industrial Research Organisation Gene expression system (particularly for rotavirus vp7 protein) involving a foreign signal peptide and optionally a transmembrane anchor sequence
ES2107454T3 (es) * 1990-01-23 1997-12-01 Tanox Biosystems Inc Segmentos extracelulares de peptidos de anclaje de la inmunoglobulina ige humana, y anticuerpos especificos para los mismos.
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface

Also Published As

Publication number Publication date
US6027910A (en) 2000-02-22
FI950042A0 (fi) 1995-01-04
WO1994001567A1 (en) 1994-01-20
PT673427E (pt) 2003-08-29
AU685057B2 (en) 1998-01-15
JPH07508652A (ja) 1995-09-28
DE69332829D1 (de) 2003-05-08
AU4565393A (en) 1994-01-31
FI950042A7 (fi) 1995-01-24
ES2196009T3 (es) 2003-12-16
CA2139670A1 (en) 1994-01-20
CA2139670C (en) 2005-09-27
DE69332829T2 (de) 2004-01-22
KR950702634A (ko) 1995-07-29
DK0673427T3 (da) 2003-07-21
OA10123A (en) 1996-12-18
JP2004254700A (ja) 2004-09-16
EP0673427B1 (de) 2003-04-02
NZ254108A (en) 1995-12-21
JP3681385B2 (ja) 2005-08-10
RU95105899A (ru) 1997-03-20
EP0673427A1 (de) 1995-09-27
FI950042L (fi) 1995-01-24

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