ES2196009T3 - Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion. - Google Patents

Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion.

Info

Publication number
ES2196009T3
ES2196009T3 ES93915827T ES93915827T ES2196009T3 ES 2196009 T3 ES2196009 T3 ES 2196009T3 ES 93915827 T ES93915827 T ES 93915827T ES 93915827 T ES93915827 T ES 93915827T ES 2196009 T3 ES2196009 T3 ES 2196009T3
Authority
ES
Spain
Prior art keywords
enzyme
protein
cell
cell wall
polinucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93915827T
Other languages
English (en)
Inventor
Franciscus Maria Klis
Maarten Pleun Schreuder
Holger York Toschka
Cornelis Theodorus Verrips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Application granted granted Critical
Publication of ES2196009T3 publication Critical patent/ES2196009T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PROPORCIONA UN METODO PARA INMOVILIZAR UNA ENZIMA, QUE COMPRENDE LA INMOVILIZACION DE UNA ENZIMA O UNA PARTE FUNCIONAL DE LA MISMA A LA PARED CELULAR DE UNA CELULA MICROBIAL, UTILIZANDO TECNICAS RECOMBINANTES DE DNA. LA ENZIMA SE INMOVILIZA UNIENDOLA AL SEGMENTO C-TERMINAL DE UNA PROTEINA QUE ASEGURA EL ANCLAJE EN LA PARED CELULAR. TAMBIEN PROPORCIONA UN POLINUCLEOTIDO RECOMBINANTE QUE COMPRENDE UN GENESTRUCTURAL QUE CODIFICA UNA PROTEINA ED ENZIMA, UNA PARTE DE UN GEN QUE CODIFICA LA PARTE C-TERMINAL DE UNA PROTEINA CAPAZ DE ANCLARSE EN UNA PARED CELULAR EUCARIOTICA O PROCARIOTICA, ADEMAS DE UNA SECUENCIA DE SEÑAL, Y EN ADICION A UNA PROTEINA QUIMERICA CODIFICADA POR EL POLINUCLEOTIDO RECOMBINANTE Y UN VECTOR Y MICRORGANISMO QUE CONTIENEN EL POLINUCLEOTIDO. EL MICRORGANISMO ES IDONEO PARA LLEVAR A CABO PROCESOS ENZIMATICOS A ESCALA INDUSTRIAL.
ES93915827T 1992-07-08 1993-07-07 Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion. Expired - Lifetime ES2196009T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92202080 1992-07-08
EP92203899 1992-12-14

Publications (1)

Publication Number Publication Date
ES2196009T3 true ES2196009T3 (es) 2003-12-16

Family

ID=26131543

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93915827T Expired - Lifetime ES2196009T3 (es) 1992-07-08 1993-07-07 Procedimiento para inmovilizar enzimas en la pared celular de una celula microbiana mediante la produccion de una proteina de fusion.

Country Status (16)

Country Link
US (1) US6027910A (es)
EP (1) EP0673427B1 (es)
JP (2) JP3681385B2 (es)
KR (1) KR950702634A (es)
AT (1) ATE236257T1 (es)
AU (1) AU685057B2 (es)
CA (1) CA2139670C (es)
DE (1) DE69332829T2 (es)
DK (1) DK0673427T3 (es)
ES (1) ES2196009T3 (es)
FI (1) FI950042A7 (es)
NZ (1) NZ254108A (es)
OA (1) OA10123A (es)
PT (1) PT673427E (es)
RU (1) RU95105899A (es)
WO (1) WO1994001567A1 (es)

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US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
DK0682710T3 (da) * 1993-02-10 2004-03-01 Unilever Nv Isolationsmetode, ved hvilken der anvendes immobiliserede proteiner med specifikke kapaciteter
CN1070533C (zh) * 1993-02-26 2001-09-05 三宝乐啤酒株式会社 酵母凝集基因及含这些基因的酵母
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
EP1163260B1 (en) 1999-03-25 2009-05-20 Valtion Teknillinen Tutkimuskeskus Process for partitioning of proteins
JP2003284556A (ja) * 1999-11-19 2003-10-07 Toyota Motor Corp 金属捕捉能タンパク質を発現する遺伝子
ES2324280T3 (es) 2000-03-14 2009-08-04 Unilever N.V. Dominios variables de la cadena pesada de anticuerpos frente a lipasas dieteticas humanas y sus usos.
DE10016279A1 (de) * 2000-04-03 2001-10-04 Henkel Kgaa Enzymatisches Färbemittel
ATE403717T1 (de) * 2000-05-01 2008-08-15 Daiichi Sankyo Co Ltd Verfahren zum screening von auf zellwände wirkenden arzneistoffen
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US20020165149A1 (en) * 2000-12-08 2002-11-07 Kranz David M. Mutated class II major histocompatibility proteins
ATE416192T1 (de) * 2001-04-19 2008-12-15 Kansai Chem Eng Protein, das sich an die zelloberflächenschichten bindet, und dessen verwendung
DK1395648T3 (da) 2001-06-11 2009-08-03 Applied Nanosystems Bv Fremgangsmåder til binding af AcmA-type proteinankerfusioner til cellevægsmateriale af mikroorganismer
DK2093286T3 (da) * 2001-10-01 2013-05-13 Dyax Corp Flerkædede, eukaryote displayvektorer og anvendelser deraf
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US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
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WO2005055944A2 (en) 2003-12-05 2005-06-23 Cincinnati Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
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JP2009501522A (ja) * 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
KR20080073293A (ko) 2005-10-14 2008-08-08 메디뮨 엘엘씨 항체 라이브러리의 세포 디스플레이
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20080138663A1 (en) * 2006-12-12 2008-06-12 Canon Kabushiki Kaisha Microbial electrode and fuel cell and sensor using the same
JP2010517577A (ja) * 2007-02-09 2010-05-27 メディミューン，エルエルシー 酵母細胞原形質膜による抗体ライブラリー提示
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EP3124497B1 (en) 2007-09-14 2020-04-15 Adimab, LLC Rationally designed, synthetic antibody libraries and uses therefor
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
ES2410579T5 (es) 2008-12-16 2021-10-04 Novartis Ag Sistemas de despliegue de levaduras
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Also Published As

Publication number Publication date
ATE236257T1 (de) 2003-04-15
US6027910A (en) 2000-02-22
AU4565393A (en) 1994-01-31
AU685057B2 (en) 1998-01-15
EP0673427B1 (en) 2003-04-02
FI950042L (fi) 1995-01-24
OA10123A (en) 1996-12-18
JPH07508652A (ja) 1995-09-28
JP3681385B2 (ja) 2005-08-10
DE69332829D1 (de) 2003-05-08
WO1994001567A1 (en) 1994-01-20
DE69332829T2 (de) 2004-01-22
PT673427E (pt) 2003-08-29
DK0673427T3 (da) 2003-07-21
EP0673427A1 (en) 1995-09-27
CA2139670C (en) 2005-09-27
CA2139670A1 (en) 1994-01-20
KR950702634A (ko) 1995-07-29
JP2004254700A (ja) 2004-09-16
NZ254108A (en) 1995-12-21
FI950042A0 (fi) 1995-01-04
FI950042A7 (fi) 1995-01-24
RU95105899A (ru) 1997-03-20

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