ATE238392T1 - Verfahren und reaktiv für einen energietransfer- assay - Google Patents
Verfahren und reaktiv für einen energietransfer- assayInfo
- Publication number
- ATE238392T1 ATE238392T1 AT99925185T AT99925185T ATE238392T1 AT E238392 T1 ATE238392 T1 AT E238392T1 AT 99925185 T AT99925185 T AT 99925185T AT 99925185 T AT99925185 T AT 99925185T AT E238392 T1 ATE238392 T1 AT E238392T1
- Authority
- AT
- Austria
- Prior art keywords
- groups
- atoms
- alkyl
- optionally
- fluorescent
- Prior art date
Links
- 238000003556 assay Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title 1
- 239000000975 dye Substances 0.000 abstract 4
- 125000004429 atom Chemical group 0.000 abstract 3
- 125000004178 (C1-C4) alkyl group Chemical group 0.000 abstract 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 2
- 125000005647 linker group Chemical group 0.000 abstract 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract 2
- 125000001424 substituent group Chemical group 0.000 abstract 2
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 abstract 2
- 125000000008 (C1-C10) alkyl group Chemical group 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract 1
- 125000004442 acylamino group Chemical group 0.000 abstract 1
- 125000003118 aryl group Chemical group 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 abstract 1
- -1 nitro, amino Chemical group 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 125000001453 quaternary ammonium group Chemical group 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 229910021653 sulphate ion Inorganic materials 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Plural Heterocyclic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Indole Compounds (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9812596.6A GB9812596D0 (en) | 1998-06-11 | 1998-06-11 | Energy transfer assay method |
| PCT/GB1999/001746 WO1999064519A1 (en) | 1998-06-11 | 1999-06-02 | Energy transfer assay method and reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE238392T1 true ATE238392T1 (de) | 2003-05-15 |
Family
ID=10833576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99925185T ATE238392T1 (de) | 1998-06-11 | 1999-06-02 | Verfahren und reaktiv für einen energietransfer- assay |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6828116B1 (de) |
| EP (1) | EP1086179B1 (de) |
| JP (1) | JP5008044B2 (de) |
| AT (1) | ATE238392T1 (de) |
| AU (1) | AU749437B2 (de) |
| CA (1) | CA2330974C (de) |
| DE (1) | DE69907185T2 (de) |
| DK (1) | DK1086179T3 (de) |
| ES (1) | ES2198133T3 (de) |
| GB (1) | GB9812596D0 (de) |
| WO (1) | WO1999064519A1 (de) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9812596D0 (en) * | 1998-06-11 | 1998-08-12 | Amersham Pharm Biotech Uk Ltd | Energy transfer assay method |
| US6627400B1 (en) | 1999-04-30 | 2003-09-30 | Aclara Biosciences, Inc. | Multiplexed measurement of membrane protein populations |
| US6322980B1 (en) | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US6673550B2 (en) | 1999-04-30 | 2004-01-06 | Aclara Biosciences, Inc. | Electrophoretic tag reagents comprising fluorescent compounds |
| US7037654B2 (en) | 1999-04-30 | 2006-05-02 | Aclara Biosciences, Inc. | Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents |
| US7001725B2 (en) | 1999-04-30 | 2006-02-21 | Aclara Biosciences, Inc. | Kits employing generalized target-binding e-tag probes |
| EP1252236A1 (de) * | 2000-02-02 | 2002-10-30 | Amersham Pharmacia Biotech UK Limited | Messreagenz |
| GB0002261D0 (en) * | 2000-02-02 | 2000-03-22 | Amersham Pharm Biotech Uk Ltd | Fluorescent detection method & reagent |
| US7160735B2 (en) | 2000-04-28 | 2007-01-09 | Monogram Biosciences, Inc. | Tagged microparticle compositions and methods |
| US7537938B2 (en) | 2000-04-28 | 2009-05-26 | Monogram Biosciences, Inc. | Biomarker detection in circulating cells |
| US7771929B2 (en) | 2000-04-28 | 2010-08-10 | Monogram Biosciences, Inc. | Tag library compounds, compositions, kits and methods of use |
| CA2423806C (en) | 2000-09-29 | 2009-12-22 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
| JP2005505749A (ja) | 2001-05-21 | 2005-02-24 | アクララ バイオサイエンシーズ， インコーポレイテッド | タンパク質を分析するための方法および組成物 |
| AU2002305658A1 (en) | 2001-05-21 | 2002-12-03 | Aclara Biosciences, Inc. | Analyzing phosphorylated proteins |
| US7358052B2 (en) | 2001-05-26 | 2008-04-15 | Monogram Biosciences, Inc. | Catalytic amplification of multiplexed assay signals |
| EP1437386A4 (de) * | 2001-09-27 | 2005-01-26 | Fuji Photo Film Co Ltd | Asymmetrischer cyaninfarbstoff |
| US20040229294A1 (en) | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB surface receptor complexes as biomarkers |
| US7402397B2 (en) | 2002-05-21 | 2008-07-22 | Monogram Biosciences, Inc. | Detecting and profiling molecular complexes |
| AU2003246957A1 (en) * | 2002-07-30 | 2004-02-16 | Amersham Biosciences Uk Limited | Site-specific labelling of proteins using cyanine dye reporters |
| JP3698708B2 (ja) * | 2003-08-07 | 2005-09-21 | 旭電化工業株式会社 | シアニン化合物、光学記録材料及び光学記録媒体 |
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| EP1720944B1 (de) | 2003-12-05 | 2013-07-17 | Life Technologies Corporation | Cyanin farbstoffverbindungen |
| WO2005083394A2 (en) * | 2004-02-20 | 2005-09-09 | Molecular Probes, Inc. | Methods for detecting anionic and non-anionic proteins using carbocyanine dyes |
| US7598390B2 (en) | 2005-05-11 | 2009-10-06 | Life Technologies Corporation | Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use |
| US8227621B2 (en) | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
| NO20053354D0 (no) * | 2005-07-11 | 2005-07-11 | Amersham Health As | Optical imaging contrast agent. |
| KR20090115944A (ko) | 2007-02-28 | 2009-11-10 | 사노피-아벤티스 | 영상 프로브 |
| EP2185721B1 (de) | 2007-08-03 | 2014-04-02 | Sanofi | Caspase-bildgebungssonden |
| DE102007038730A1 (de) * | 2007-08-16 | 2009-02-19 | Carl Zeiss Meditec Ag | Nachweis des menschlichen Vascular Endothelial Growth Factor |
| CA2706763C (en) | 2007-11-27 | 2013-07-30 | Laboratory Corporation Of America Holdings | Enhanced method for detecting and/or quantifying an analyte in a sample |
| DE102008040513B4 (de) * | 2008-07-17 | 2010-08-26 | BAM Bundesanstalt für Materialforschung und -prüfung | Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen |
| SG172321A1 (en) | 2009-01-29 | 2011-07-28 | Commw Scient Ind Res Org | Measuring g protein coupled receptor activation |
| US8865075B2 (en) * | 2010-11-01 | 2014-10-21 | Princeton Biochemicals, Inc. | Multi-task immunoaffinity device secured to a peripheral box and integrated to a capillary electrophoresis apparatus |
| WO2015064786A1 (ko) * | 2013-10-31 | 2015-05-07 | 주식회사 디케이씨코포레이션 | 생체 분자 표지를 위한 시아닌 염료 및 그 제조방법 |
| GB201516987D0 (en) | 2015-09-25 | 2015-11-11 | Illumina Cambridge Ltd | Polymethine compounds and their use as fluorescent labels |
| CN118475563A (zh) * | 2022-05-07 | 2024-08-09 | 宁波康柏睿格医药科技有限公司 | 花菁素-海藻糖类化合物、其制备方法及用途 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT988269B (it) * | 1973-06-18 | 1975-04-10 | Minnesota Mining & Mfg | Coloranti cianinici e loro impiego come sensibilizzatori spettrali in materiali fotografici positivi diretti del tipo ad effetto herschel sensibilizzato spettralmente |
| JPS55116259A (en) * | 1979-03-01 | 1980-09-06 | Fuji Photo Film Co Ltd | Microimmunoassay method |
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| DE3912046B4 (de) * | 1988-09-02 | 2004-03-25 | Carnegie Mellon University | Verfahren zum Markieren einer Komponente einer wäßrigen Flüssigkeit und lumineszenzphotostabiles Reaktionsprodukt |
| US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| JPH075618A (ja) * | 1993-06-18 | 1995-01-10 | Fuji Photo Film Co Ltd | 直接ポジ型ハロゲン化銀写真感光材料 |
| PL328272A1 (en) * | 1996-01-23 | 1999-01-18 | Rapigene Inc | Methods of and compositions for detecting ligand pair bonds using non-fluoroscent labelling |
| EP0898596B1 (de) * | 1996-04-19 | 2001-08-29 | Amersham Pharmacia Biotech UK Limited | Quadratfarbstoffe und deren verwendung in einem fluorescenzsequenzierungsverfahren |
| JPH10119434A (ja) * | 1996-10-23 | 1998-05-12 | Sony Corp | 光記録媒体 |
| DE19649971A1 (de) * | 1996-11-19 | 1998-05-28 | Diagnostikforschung Inst | Optische Diagnostika zur Diagnostik neurodegenerativer Krankheiten mittels Nahinfrarot-Strahlung (NIR-Strahlung) |
| EP1000359A1 (de) * | 1998-05-26 | 2000-05-17 | Koninklijke Philips Electronics N.V. | Marker zur erzeugung einer charge-transfer fluoreszenzsonde |
| GB9812596D0 (en) * | 1998-06-11 | 1998-08-12 | Amersham Pharm Biotech Uk Ltd | Energy transfer assay method |
| GB9901746D0 (en) | 1999-01-27 | 1999-03-17 | Rom Limited | Cage former and clamp therefor |
-
1998
- 1998-06-11 GB GBGB9812596.6A patent/GB9812596D0/en not_active Ceased
-
1999
- 1999-06-02 ES ES99925185T patent/ES2198133T3/es not_active Expired - Lifetime
- 1999-06-02 JP JP2000553515A patent/JP5008044B2/ja not_active Expired - Fee Related
- 1999-06-02 AT AT99925185T patent/ATE238392T1/de not_active IP Right Cessation
- 1999-06-02 WO PCT/GB1999/001746 patent/WO1999064519A1/en not_active Ceased
- 1999-06-02 DK DK99925185T patent/DK1086179T3/da active
- 1999-06-02 DE DE69907185T patent/DE69907185T2/de not_active Expired - Lifetime
- 1999-06-02 CA CA002330974A patent/CA2330974C/en not_active Expired - Fee Related
- 1999-06-02 EP EP99925185A patent/EP1086179B1/de not_active Expired - Lifetime
- 1999-06-02 AU AU41568/99A patent/AU749437B2/en not_active Ceased
- 1999-06-03 US US09/719,016 patent/US6828116B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU4156899A (en) | 1999-12-30 |
| DE69907185T2 (de) | 2004-03-11 |
| JP2003532745A (ja) | 2003-11-05 |
| CA2330974A1 (en) | 1999-12-16 |
| CA2330974C (en) | 2008-10-21 |
| EP1086179A1 (de) | 2001-03-28 |
| ES2198133T3 (es) | 2004-01-16 |
| WO1999064519A1 (en) | 1999-12-16 |
| DE69907185D1 (de) | 2003-05-28 |
| EP1086179B1 (de) | 2003-04-23 |
| US6828116B1 (en) | 2004-12-07 |
| JP5008044B2 (ja) | 2012-08-22 |
| DK1086179T3 (da) | 2003-08-11 |
| GB9812596D0 (en) | 1998-08-12 |
| AU749437B2 (en) | 2002-06-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1086179 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |