ATE263841T1 - Praktische in vitro sialylierung von rekombinanten glykpproteinen - Google Patents

Praktische in vitro sialylierung von rekombinanten glykpproteinen

Info

Publication number
ATE263841T1
ATE263841T1 AT98904583T AT98904583T ATE263841T1 AT E263841 T1 ATE263841 T1 AT E263841T1 AT 98904583 T AT98904583 T AT 98904583T AT 98904583 T AT98904583 T AT 98904583T AT E263841 T1 ATE263841 T1 AT E263841T1
Authority
AT
Austria
Prior art keywords
practical
glyp
recombinant
proteins
sialylation
Prior art date
Application number
AT98904583T
Other languages
English (en)
Inventor
James C Paulson
Robert J Bayer
Eric Sjoberg
Original Assignee
Neose Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neose Technologies Inc filed Critical Neose Technologies Inc
Application granted granted Critical
Publication of ATE263841T1 publication Critical patent/ATE263841T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT98904583T 1997-01-16 1998-01-15 Praktische in vitro sialylierung von rekombinanten glykpproteinen ATE263841T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3571097P 1997-01-16 1997-01-16
PCT/US1998/000835 WO1998031826A1 (en) 1997-01-16 1998-01-15 Practical in vitro sialylation of recombinant glycoproteins

Publications (1)

Publication Number Publication Date
ATE263841T1 true ATE263841T1 (de) 2004-04-15

Family

ID=21884346

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98904583T ATE263841T1 (de) 1997-01-16 1998-01-15 Praktische in vitro sialylierung von rekombinanten glykpproteinen

Country Status (12)

Country Link
US (5) US6399336B1 (de)
EP (1) EP1015622B1 (de)
JP (2) JP2001507215A (de)
AT (1) ATE263841T1 (de)
AU (1) AU736993B2 (de)
CA (1) CA2278178C (de)
DE (1) DE69823046T2 (de)
DK (1) DK1015622T3 (de)
ES (1) ES2218806T3 (de)
IL (2) IL130964A0 (de)
NZ (1) NZ336780A (de)
WO (1) WO1998031826A1 (de)

Families Citing this family (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ336780A (en) * 1997-01-16 2000-12-22 Neose Technologies Inc In vitro sialylation of glycoproteins
US6689604B1 (en) * 1998-03-20 2004-02-10 National Research Council Of Canada Lipopolysaccharide α-2,3 sialyltransferase of Campylobacter jejuni and its uses
NZ512185A (en) 1998-12-09 2004-02-27 Dow Chemical Co A method for manufacturing glycoproteins having human- type glycosylation in plant cells
US7345019B1 (en) * 1999-04-13 2008-03-18 The Kenneth S. Warren Institute, Inc. Modulation of excitable tissue function by peripherally administered erythropoietin
AU1738401A (en) 1999-10-26 2001-05-08 Plant Research International B.V. Mammalian-type glycosylation in plants
AU2001263149A1 (en) * 2000-05-12 2001-11-26 Neose Technologies, Inc. In vitro fucosylation recombinant glycopeptides
US20030072737A1 (en) * 2000-12-29 2003-04-17 Michael Brines Tissue protective cytokines for the protection, restoration, and enhancement of responsive cells, tissues and organs
US7767643B2 (en) 2000-12-29 2010-08-03 The Kenneth S. Warren Institute, Inc. Protection, restoration, and enhancement of erythropoietin-responsive cells, tissues and organs
CA2434364C (en) 2001-01-19 2013-03-19 The Dow Chemical Company Method for secretory production of glycoprotein having human-type sugar chain using plant cell
US7125843B2 (en) 2001-10-19 2006-10-24 Neose Technologies, Inc. Glycoconjugates including more than one peptide
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7439043B2 (en) 2001-10-10 2008-10-21 Neose Technologies, Inc. Galactosyl nucleotide sugars
US7696163B2 (en) 2001-10-10 2010-04-13 Novo Nordisk A/S Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7157277B2 (en) 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
US7795210B2 (en) 2001-10-10 2010-09-14 Novo Nordisk A/S Protein remodeling methods and proteins/peptides produced by the methods
US7265085B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycoconjugation methods and proteins/peptides produced by the methods
US7179617B2 (en) 2001-10-10 2007-02-20 Neose Technologies, Inc. Factor IX: remolding and glycoconjugation of Factor IX
US7399613B2 (en) 2001-10-10 2008-07-15 Neose Technologies, Inc. Sialic acid nucleotide sugars
US8008252B2 (en) * 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
US7265084B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
US7226903B2 (en) 2001-10-10 2007-06-05 Neose Technologies, Inc. Interferon beta: remodeling and glycoconjugation of interferon beta
US7297511B2 (en) 2001-10-10 2007-11-20 Neose Technologies, Inc. Interferon alpha: remodeling and glycoconjugation of interferon alpha
EP2042196B1 (de) 2001-10-10 2016-07-13 ratiopharm GmbH Neumodellierung und Glykokonjugation von Granulozyten-Kolonie stimulierender Faktor (G-CSF)
UA87433C2 (ru) * 2001-10-22 2009-07-27 Еплайд Рісьорч Системз Ерс Холдінг Н.В. Гонадотропины для стимулирования фолликулогенеза
DE60228492D1 (de) 2001-11-28 2008-10-02 Neose Technologies Inc Remodellierung von glycoproteinen unter verwendung von endoglycanasen
US7473680B2 (en) 2001-11-28 2009-01-06 Neose Technologies, Inc. Remodeling and glycoconjugation of peptides
EP2339004B1 (de) 2002-03-19 2015-02-25 Stichting Dienst Landbouwkundig Onderzoek Optimierung von Glycanprozessierung in Pflanzen
EP2319935B1 (de) 2002-03-19 2016-04-27 Stichting Dienst Landbouwkundig Onderzoek GnTIII (UDP-N-acetylglucosamin:Beta -D mannoside Beta (1,4)-N -acetylglucosaminyltransferase III) expression in pflanzen
MXPA04012496A (es) 2002-06-21 2005-09-12 Novo Nordisk Healthcare Ag Glicoformos del factor vii pegilados.
BR0311978A (pt) 2002-06-21 2005-03-22 Novo Nordisk Healthcare Ag Preparação, método para preparar a mesma, formulação farmacêutica, métodos para tratar de uma sìndrome responsiva ao fator vii, para prevenir hemorragias indesejáveis, para prevenir coagulação sanguìnea indesejável e para prevenir as reações mediadas pelo fator tecidual, e, uso de uma preparação
US20040122216A1 (en) * 2002-07-01 2004-06-24 Jacob Nielsen Recombinant tissue protective cytokines and encoding nucleic acids thereof for protection, restoration, and enhancement of responsive cells, tissues, and organs
AU2003301332B2 (en) * 2002-10-17 2008-06-12 Pharming Intellectual Property B.V. Protein modification
CL2003002461A1 (es) 2002-11-27 2005-01-07 Dow Chemical Company Agroscien Inmunoglobulina que comprende al menos un glicano afucosilado, composicion que la contiene, secuencia nucleotidica y vector que la comprende, procedimiento para producir dicha inmunoglobulina en plantas.
JP2006523211A (ja) 2003-03-14 2006-10-12 ネオス テクノロジーズ インコーポレイテッド 分岐水溶性ポリマーとその複合物
EP1613261A4 (de) * 2003-04-09 2011-01-26 Novo Nordisk As Intrazelluläre bildung von peptidkonjugaten
WO2007022512A2 (en) * 2005-08-19 2007-02-22 Neose Technologies, Inc. Glycopegylated factor vii and factor viia
US8791070B2 (en) 2003-04-09 2014-07-29 Novo Nordisk A/S Glycopegylated factor IX
WO2006127896A2 (en) 2005-05-25 2006-11-30 Neose Technologies, Inc. Glycopegylated factor ix
NZ542586A (en) 2003-04-09 2009-11-27 Biogenerix Ag Method of forming a covalent conjugate between a G-CSF peptide and PEG
AU2004240553A1 (en) * 2003-05-09 2004-12-02 Neose Technologies, Inc. Compositions and methods for the preparation of human growth hormone glycosylation mutants
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
JP2007517769A (ja) * 2003-09-29 2007-07-05 ウォーレン ファーマシューティカルズ，インコーポレーテッド 敗血症および癒着形成の治療および予防のための組織保護性サイトカイン
US7405198B2 (en) * 2003-11-24 2008-07-29 Neose Technologies, Inc. Glycopegylated erythropoietin
WO2005072371A2 (en) * 2004-01-26 2005-08-11 Neose Technologies, Inc. Branched polymeric sugars and nucleotides thereof
US8633157B2 (en) 2003-11-24 2014-01-21 Novo Nordisk A/S Glycopegylated erythropoietin
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
US20060040856A1 (en) 2003-12-03 2006-02-23 Neose Technologies, Inc. Glycopegylated factor IX
US7956032B2 (en) 2003-12-03 2011-06-07 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
US7338933B2 (en) 2004-01-08 2008-03-04 Neose Technologies, Inc. O-linked glycosylation of peptides
KR20070008645A (ko) 2004-05-04 2007-01-17 노보 노르디스크 헬스 케어 악티엔게젤샤프트 폴리펩티드의 ｏ-연결된 단백당형 및 그들의 제조 방법
WO2006010143A2 (en) 2004-07-13 2006-01-26 Neose Technologies, Inc. Branched peg remodeling and glycosylation of glucagon-like peptide-1 [glp-1]
US20090292110A1 (en) * 2004-07-23 2009-11-26 Defrees Shawn Enzymatic modification of glycopeptides
EP1799249A2 (de) 2004-09-10 2007-06-27 Neose Technologies, Inc. Glycopegyliertes interferon alpha
JP4812625B2 (ja) * 2004-10-18 2011-11-09 日本たばこ産業株式会社 糖転移酵素の酵素活性を向上させる方法
EP3061461A1 (de) 2004-10-29 2016-08-31 ratiopharm GmbH Remodellierung und glykopegylierung von fibroblasten-wachstumsfaktor (fgf)
WO2006072625A2 (en) 2005-01-06 2006-07-13 Novo Nordisk A/S Anti-kir combination treatments and methods
JP2008526864A (ja) * 2005-01-06 2008-07-24 ネオス テクノロジーズ インコーポレイテッド 糖断片を用いる糖結合
US9029331B2 (en) 2005-01-10 2015-05-12 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
WO2006102652A2 (en) * 2005-03-24 2006-09-28 Neose Technologies, Inc. Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms
EP1871795A4 (de) * 2005-04-08 2010-03-31 Biogenerix Ag Zusammensetzungen und verfahren zur herstellung von glycosylierungsmutanten eines proteaseresistenten menschlichen wachstumshormons
WO2006127910A2 (en) 2005-05-25 2006-11-30 Neose Technologies, Inc. Glycopegylated erythropoietin formulations
US20110003744A1 (en) * 2005-05-25 2011-01-06 Novo Nordisk A/S Glycopegylated Erythropoietin Formulations
FI20055398A0 (fi) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Menetelmä solupopulaatioiden evaluoimiseksi
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
WO2007028106A2 (en) 2005-08-31 2007-03-08 Centocor, Inc. Host cell lines for production of antibody constant region with enhanced effector function
WO2007056191A2 (en) 2005-11-03 2007-05-18 Neose Technologies, Inc. Nucleotide sugar purification using membranes
FR2901707B1 (fr) * 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
AR078117A1 (es) 2006-06-20 2011-10-19 Protech Pharma S A Una muteina recombinante del interferon alfa humano glicosilado, un gen que codifica para dicha muteina, un metodo de produccion de dicho gen, un metodo para obtener una celula eucariota productora de dicha muteina, un metodo para producir dicha muteina, un procedimiento para purificar dicha muteina
US20080242607A1 (en) 2006-07-21 2008-10-02 Neose Technologies, Inc. Glycosylation of peptides via o-linked glycosylation sequences
FR2904558B1 (fr) * 2006-08-01 2008-10-17 Lab Francais Du Fractionnement "composition de facteur vii recombinant ou transgenique, presentant majoritairement des formes glycanniques biantennees, bisialylees et non fucosylees"
US8969532B2 (en) 2006-10-03 2015-03-03 Novo Nordisk A/S Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
BRPI0717505B8 (pt) 2006-10-04 2021-05-25 Novo Nordisk As conjugado de peptídeo e formulação farmacêutica
JP2010512306A (ja) * 2006-10-27 2010-04-22 ザ ロックフェラー ユニバーシティー 抗炎症特性が増強され、細胞毒性特性が減少したポリペプチドおよび関連する方法
FI20075030A0 (fi) * 2007-01-18 2007-01-18 Suomen Punainen Risti Veripalv Menetelmä solujen modifioimiseksi
CN101796063B (zh) 2007-04-03 2017-03-22 拉蒂奥法姆有限责任公司 使用糖聚乙二醇化g‑csf的治疗方法
NZ580510A (en) 2007-04-17 2011-06-30 Stichting Dienst Landbouwkundi Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
CA2683423C (en) * 2007-04-26 2020-10-27 Inspiration Biopharmaceuticals, Inc. Recombinant vitamin k dependent proteins with high sialic acid content and methods of preparing same
US9493499B2 (en) 2007-06-12 2016-11-15 Novo Nordisk A/S Process for the production of purified cytidinemonophosphate-sialic acid-polyalkylene oxide (CMP-SA-PEG) as modified nucleotide sugars via anion exchange chromatography
US7968811B2 (en) * 2007-06-29 2011-06-28 Harley-Davidson Motor Company Group, Inc. Integrated ignition and key switch
DK2197919T3 (da) 2007-08-27 2014-07-07 Ratiopharm Gmbh Flydende formulering af g-csf-konjugat
US8207112B2 (en) 2007-08-29 2012-06-26 Biogenerix Ag Liquid formulation of G-CSF conjugate
DK2257311T3 (da) 2008-02-27 2014-06-30 Novo Nordisk As Konjugerede Faktor VIII-Molekyler
TWI488640B (zh) 2008-04-16 2015-06-21 菲瑞茵國際中心股份有限公司 藥學製劑
EP2166085A1 (de) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalente modifizierte Zellen
EP2347003B1 (de) 2008-10-29 2020-06-10 Janssen Biotech, Inc. Galactose-alpha (1-3)-galactose-zusammensetzungen
WO2011101277A1 (en) 2010-02-16 2011-08-25 Novo Nordisk A/S Conjugated proteins
BR112014007766B1 (pt) 2011-10-01 2022-05-10 Glytech, Inc Polipeptídeo glicosilado, método para fabricação do mesmo e composição farmacêutica
CA2865170C (en) 2012-02-21 2020-12-22 Cytonics Corporation Systems, compositions, and methods for transplantation
EP2877589B1 (de) 2012-07-26 2024-03-06 Momenta Pharmaceuticals, Inc. Glykoproteine mit entzündungshemmenden eigenschaften
MY171183A (en) * 2013-03-29 2019-09-30 Glytech Inc Polypeptide glycosylated with sialylated sugar chain
US10464996B2 (en) 2013-05-13 2019-11-05 Momenta Pharmaceuticals, Inc. Methods for the treatment of neurodegeneration
WO2015031654A2 (en) 2013-08-28 2015-03-05 Cytonics Corporation Systems, compositions, and methods for transplantation and treating conditions
WO2015057622A1 (en) 2013-10-16 2015-04-23 Momenta Pharmaceuticals, Inc. Sialylated glycoproteins
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10889631B2 (en) 2014-11-20 2021-01-12 Cytonics Corporation Therapeutic variant alpha-2-macroglobulin compositions
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US10772936B2 (en) * 2015-05-22 2020-09-15 CSL Behring Lengnau AG Methods for preparing modified von Willebrand factor
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
KR102229037B1 (ko) 2017-09-04 2021-03-17 89바이오 리미티드 돌연변이 fgf-21 펩티드 접합체 및 이의 용도
WO2020204814A1 (en) * 2019-03-29 2020-10-08 Agency For Science, Technology And Research Method of sialylating a protein

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272066A (en) 1986-03-07 1993-12-21 Massachusetts Institute Of Technology Synthetic method for enhancing glycoprotein stability
JPS63258591A (ja) * 1987-04-17 1988-10-26 Oji Paper Co Ltd 配糖体の製造方法
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
US5541083A (en) 1989-10-24 1996-07-30 The Regents Of The University Of California Method for producing secretable glycosyltransferases and other golgi processing enzymes
AU668505B2 (en) 1991-03-18 1996-05-09 Scripps Research Institute, The Oligosaccharide enzyme substrates and inhibitors: methods and compositions
US5352670A (en) * 1991-06-10 1994-10-04 Alberta Research Council Methods for the enzymatic synthesis of alpha-sialylated oligosaccharide glycosides
JPH05166757A (ja) * 1991-12-13 1993-07-02 Tokyo Electron Ltd 被処理体の温調装置
GB9225054D0 (en) * 1992-11-30 1993-01-20 Baxenden Chem Enzymatic synthesis
US5374541A (en) * 1993-05-04 1994-12-20 The Scripps Research Institute Combined use of β-galactosidase and sialyltransferase coupled with in situ regeneration of CMP-sialic acid for one pot synthesis of oligosaccharides
US5409817A (en) 1993-05-04 1995-04-25 Cytel, Inc. Use of trans-sialidase and sialyltransferase for synthesis of sialylα2→3βgalactosides
US5834251A (en) * 1994-12-30 1998-11-10 Alko Group Ltd. Methods of modifying carbohydrate moieties
ES2180757T3 (es) * 1995-04-11 2003-02-16 Neose Technologies Inc Sintesis enzimatica mejorada de oligosacaridos.
NZ336780A (en) * 1997-01-16 2000-12-22 Neose Technologies Inc In vitro sialylation of glycoproteins

Also Published As

Publication number Publication date
AU736993B2 (en) 2001-08-09
IL130964A0 (en) 2001-01-28
US20030124645A1 (en) 2003-07-03
EP1015622B1 (de) 2004-04-07
AU6242798A (en) 1998-08-07
US6399336B1 (en) 2002-06-04
DK1015622T3 (da) 2004-08-02
US20020119516A1 (en) 2002-08-29
WO1998031826A1 (en) 1998-07-23
DE69823046D1 (de) 2004-05-13
CA2278178A1 (en) 1998-07-23
DE69823046T2 (de) 2005-03-31
US20020142370A1 (en) 2002-10-03
NZ336780A (en) 2000-12-22
EP1015622A4 (de) 2002-06-19
ES2218806T3 (es) 2004-11-16
US7220555B2 (en) 2007-05-22
IL130964A (en) 2006-06-11
JP2011024597A (ja) 2011-02-10
CA2278178C (en) 2012-09-25
JP2001507215A (ja) 2001-06-05
EP1015622A1 (de) 2000-07-05
US20020160460A1 (en) 2002-10-31

Similar Documents

Publication Publication Date Title
DE69823046D1 (de) Praktische in vitro sialylierung von rekombinanten glykpproteinen
ATE339507T1 (de) Erythropoietin-analog-menschliches serum-albumin fusionsprotein
DK0757717T3 (da) Papillomavirusvacciner
DE3884529D1 (de) Leader-anteile zur produktion von rekombinanten proteinen.
DE60228492D1 (de) Remodellierung von glycoproteinen unter verwendung von endoglycanasen
BR0013231A (pt) Complexos citocina-anticorpo múltiplos
TR199700610A2 (xx) D���k molek�l a��rl�kl� ��z�n�r, t�m�r nekroz fakt�r� tip-I ve tip-II proteinleri.
BR9507618A (pt) Frações polipeptidicas solúveis da proteína lag-3 processo de produção composição terapêutica anticorpos anti-idiotipo
ATE317904T1 (de) Überexpression von phytase in hefesystemen
DE69535147D1 (de) Überexpression von säugetier- und virusproteinen
PT1121382E (pt) Proteinas de fusao do interferao beta e as respectivas utilizacoes
DK289285A (da) Thymopentin analoge
EE03693B1 (et) Isoleeritud dimeerne fibroblastide aktivatsioonivalk alfa ja selle kasutusalad
ES2196082T3 (es) Analagos del factor de crecimiento de queratinocitos.
ATE223481T1 (de) Peptid der löslichen form von acetylcholinesterase mit aktivität als calciumkanalmodulator
ATE285474T1 (de) Haemophilus adhäsionsproteinen
DE69032258D1 (de) Antikoagulierende Peptide
ATE397081T1 (de) Erhöhte expression von proteinen mittels gfp
DK0942992T3 (da) O-fucosyltransferase
AP9100296A0 (en) Peptides and analogues and mixtures thereof for detecting antibodies to HTLV-1 and HTLV11 viruses.
DE68928942D1 (de) Antikoagulierende Peptide
FI943218A7 (fi) Kaseinomakropeptidin peptidit, mainittujen peptidien vasta-aineet ja k äytöt
DE69122103D1 (de) Antigerinnungspeptide
NO20020329D0 (no) Antitumor antistoffer, proteiner og anvendelser derav
DE69000908D1 (de) Leichtes transportables brueckenteil zur ueberwindung von graeben.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1015622

Country of ref document: EP

REN Ceased due to non-payment of the annual fee