ATE295412T1 - Methoden für die ex-vivo replikation und stabile genetische transformation von menschlichen stamm- zellen - Google Patents

Methoden für die ex-vivo replikation und stabile genetische transformation von menschlichen stamm- zellen

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Publication number
ATE295412T1
ATE295412T1 AT92904136T AT92904136T ATE295412T1 AT E295412 T1 ATE295412 T1 AT E295412T1 AT 92904136 T AT92904136 T AT 92904136T AT 92904136 T AT92904136 T AT 92904136T AT E295412 T1 ATE295412 T1 AT E295412T1
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Austria
Prior art keywords
methods
human stem
stem cells
genetic transformation
stable genetic
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AT92904136T
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English (en)
Inventor
Stephen G Emerson
Michael F Clarke
Bernhard O Palsson
Richard M Schwartz
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Univ Michigan
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Publication of ATE295412T1 publication Critical patent/ATE295412T1/de

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AT92904136T 1990-12-17 1991-12-17 Methoden für die ex-vivo replikation und stabile genetische transformation von menschlichen stamm- zellen ATE295412T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US62834390A 1990-12-17 1990-12-17
US73702491A 1991-07-29 1991-07-29
US07/740,590 US5399493A (en) 1989-06-15 1991-08-05 Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
PCT/US1991/009173 WO1992011355A1 (en) 1990-12-17 1991-12-17 Method for culturing and transforming human stem cell-containing compositions

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ATE295412T1 true ATE295412T1 (de) 2005-05-15

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US (2) US5399493A (de)
EP (2) EP0575350B1 (de)
JP (5) JPH06505151A (de)
KR (1) KR100225307B1 (de)
AT (1) ATE295412T1 (de)
AU (1) AU687674B2 (de)
CA (1) CA2100268C (de)
DE (1) DE69133459T2 (de)
WO (1) WO1992011355A1 (de)

Families Citing this family (197)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69012601T2 (de) * 1989-02-02 1995-01-19 Joel S Greenberger Gentherapie durch stützgewebebezügliche Zellen.
US5399493A (en) * 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
US5635386A (en) 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5605822A (en) * 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
US5635387A (en) * 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
ATE164076T1 (de) * 1990-06-08 1998-04-15 Univ New York Stimulierung der stroma- und vorläuferzellen im knochenmark
US5612211A (en) * 1990-06-08 1997-03-18 New York University Stimulation, production and culturing of hematopoietic progenitor cells by fibroblast growth factors
US20030125519A1 (en) 1990-08-27 2003-07-03 Peter Besmer Ligand for the c-kit receptor and methods of use thereof
US5744361A (en) * 1991-04-09 1998-04-28 Indiana University Expansion of human hematopoietic progenitor cells in a liquid medium
US5199942A (en) * 1991-06-07 1993-04-06 Immunex Corporation Method for improving autologous transplantation
NL9101680A (nl) * 1991-10-04 1993-05-03 Tno Werkwijze voor het genetisch modificeren van beenmergcellen van primaten, alsmede daarbij bruikbare cellen die recombinante retrovirale vectoren produceren.
JPH07504570A (ja) * 1992-03-04 1995-05-25 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン ヒトの幹細胞および/または造血細胞を維持,生育するための方法,組成物および装置
CA2109729A1 (en) * 1992-03-23 1993-09-30 James G. Bender In vitro-derived human neutrophil precursor cells
US6001803A (en) * 1992-04-23 1999-12-14 Sloan-Kettering Institute For Cancer Research Composition of c-kit ligand, GM-CSF, and TNF-α and method of use
ES2168273T3 (es) * 1992-04-23 2002-06-16 Sloan Kettering Inst Cancer Ligando para el receptor c-kit y metodos de uso del mismo.
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5554512A (en) * 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
CZ307995A3 (en) 1993-05-24 1996-10-16 Immunex Corp Ligands for flt3 receptors
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
US5846529A (en) * 1993-08-23 1998-12-08 Nexell Therapeutics, Inc. Infusion of neutrophil precursors for treatment of neutropenia
FR2709309B1 (fr) * 1993-08-25 1995-11-10 Centre Nat Rech Scient Compositions cellulaires, préparation et utilisations thérapeutiques.
US5534423A (en) * 1993-10-08 1996-07-09 Regents Of The University Of Michigan Methods of increasing rates of infection by directing motion of vectors
US5599705A (en) * 1993-11-16 1997-02-04 Cameron; Robert B. In vitro method for producing differentiated universally compatible mature human blood cells
US6190655B1 (en) 1993-12-03 2001-02-20 Immunex Corporation Methods of using Flt-3 ligand for exogenous gene transfer
US5622857A (en) * 1995-08-08 1997-04-22 Genespan Corporation High performance cell culture bioreactor and method
US5882918A (en) * 1995-08-08 1999-03-16 Genespan Corporation Cell culture incubator
AU687059B2 (en) * 1994-02-09 1998-02-19 Genespan Corporation High performance cell culture bioreactor and method
US7294331B2 (en) * 1994-03-07 2007-11-13 Immunex Corporation Methods of using flt3-ligand in hematopoietic cell transplantation
US6242417B1 (en) 1994-03-08 2001-06-05 Somatogen, Inc. Stabilized compositions containing hemoglobin
US5631219A (en) * 1994-03-08 1997-05-20 Somatogen, Inc. Method of stimulating hematopoiesis with hemoglobin
US5512480A (en) * 1994-03-11 1996-04-30 Baxter International Inc. Flow-through bioreactor with grooves for cell retention
WO1995024464A1 (en) * 1994-03-11 1995-09-14 Baxter International Inc. Flow-through bioreactor with grooves for cell retention
US7083979B1 (en) * 1994-03-25 2006-08-01 Indiana University Foundation Methods for enhanced retroviral-mediated gene transfer
DE4422667A1 (de) * 1994-06-30 1996-01-04 Boehringer Ingelheim Int Verfahren zur Herstellung und Züchtung hämatopoetischer Vorläuferzellen
WO1996017627A2 (en) * 1994-12-09 1996-06-13 Mosca Joseph D System for the maintenance, growth and differentiation of human and non-human primate pluripotent stem, progenitor and mature bone marrow cells
US5811274A (en) * 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
FR2728584A1 (fr) 1994-12-22 1996-06-28 Centre Nat Rech Scient Procede de transfert de gene dans des cellules activees a partir d'un etat de repos
US6974571B2 (en) * 1995-03-28 2005-12-13 Thomas Jefferson University Isolated stromal cells and methods of using the same
US5719058A (en) * 1995-04-10 1998-02-17 Merck & Co., Inc. Method for producing a highly enriched population of osteoclast cells
US6121042A (en) * 1995-04-27 2000-09-19 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
US5846828A (en) * 1995-06-07 1998-12-08 Advanced Tissue Sciences Apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing tissue, synthetic, or mechanical heart valves orvalve segments
US5876956A (en) * 1995-05-15 1999-03-02 Johns Hopkins University School Of Medicine Methods for identification or purification of cells containing an enzymatic intracellular marker
US5674750A (en) * 1995-05-19 1997-10-07 T. Breeders Continuous selective clonogenic expansion of relatively undifferentiated cells
US5925567A (en) * 1995-05-19 1999-07-20 T. Breeders, Inc. Selective expansion of target cell populations
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
US5688687A (en) * 1995-06-07 1997-11-18 Aastrom Biosciences, Inc. Bioreactor for mammalian cell growth and maintenance
AU5931896A (en) * 1995-06-07 1996-12-30 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, st oring, shipping, and testing replacement cartilage tissue co nstructs
US5843766A (en) * 1995-06-07 1998-12-01 Advanced Tissue Sciences, Inc. Apparatus for the growth and packaging of three dimensional tissue cultures
GB9518606D0 (en) 1995-09-12 1995-11-15 Inst Of Psychiatry Neural transplantation
US7150992B1 (en) 1995-10-04 2006-12-19 Innunex Corporation Methods of preparing dendritic cells with flt3-ligand and antigen
US7361330B2 (en) * 1995-10-04 2008-04-22 Immunex Corporation Methods of using flt3-ligand in the treatment of fibrosarcoma
US20020034517A1 (en) * 1995-10-04 2002-03-21 Kenneth Brasel Dendritic cell stimulatory factor
US6060052A (en) * 1995-10-30 2000-05-09 Systemix, Inc. Methods for use of Mpl ligands with primitive human hematopoietic stem cells
US6991787B1 (en) 1995-12-29 2006-01-31 Alg Company Methods of preparing bone marrow stromal cells for use in gene therapy
CN1209836A (zh) * 1995-12-29 1999-03-03 阿尔格公司 骨髓基质细胞的扩展
US5763267A (en) * 1996-04-16 1998-06-09 Advanced Tissue Sciences Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures
US6165785A (en) * 1996-05-24 2000-12-26 University Of Cincinnati Bone marrow cultures for developing suppressor and stimulator cells for research and therapeutic applications
ATE299030T1 (de) * 1996-07-10 2005-07-15 Meiji Dairies Corp Verwendung von proteinen aus der mk familie als hämatopoietischer faktor
US6458585B1 (en) 1996-08-14 2002-10-01 Nexell Therapeutics Inc. Cytokine-free culture of dendritic cells
EA199900355A1 (ru) 1996-10-01 2000-08-28 Джерон Корпорейшн Теломераза обратная тринскриптаза
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
AU7481098A (en) * 1997-05-13 1998-12-08 Case Western Reserve University Osteoarthritis cartilage regeneration using human mesenchymal stem ce lls
US20020034819A1 (en) * 1998-02-23 2002-03-21 Alan K. Smith Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses
US6835377B2 (en) 1998-05-13 2004-12-28 Osiris Therapeutics, Inc. Osteoarthritis cartilage regeneration
ES2296413T3 (es) * 1998-06-08 2008-04-16 Osiris Therapeutics, Inc. Regulacion de la diferenciacion de celulas madre hematopoyeticas por el uso de celulas madre mesenquimaticas humanas.
AUPP421298A0 (en) * 1998-06-19 1998-07-09 Fertilitescentrum Ab Method and medium for in vitro culture of human embryos
EP1105463A1 (de) * 1998-08-24 2001-06-13 t. Breeders, Inc. Hemangioblasten enthaltender zellverband und dessen herstellungsverfahren
GB9904281D0 (en) * 1999-02-24 1999-04-21 Reneuron Ltd Transplantation
US20040241842A1 (en) * 1999-04-15 2004-12-02 Monash University Stimulation of thymus for vaccination development
US20040259803A1 (en) * 1999-04-15 2004-12-23 Monash University Disease prevention by reactivation of the thymus
US20040258672A1 (en) * 1999-04-15 2004-12-23 Monash University Graft acceptance through manipulation of thymic regeneration
AUPR074500A0 (en) * 2000-10-13 2000-11-09 Monash University Treatment of t cell disorders
US20050020524A1 (en) * 1999-04-15 2005-01-27 Monash University Hematopoietic stem cell gene therapy
US20070274946A1 (en) * 1999-04-15 2007-11-29 Norwood Immunoloty, Ltd. Tolerance to Graft Prior to Thymic Reactivation
US20040265285A1 (en) * 1999-04-15 2004-12-30 Monash University Normalization of defective T cell responsiveness through manipulation of thymic regeneration
EP1224259A4 (de) * 1999-09-27 2005-04-27 Univ Florida Rückbildung von insulinabhängigem diabetes durch inselbildende stammzellen, inselvorläuferzellen und inselähnliche strukturen
EP1246617B1 (de) * 1999-12-06 2006-09-13 Rhode Island Hospital Verwendung von taurolidin oder taurultam zur herstellung eines medikaments zur behandlung von ovarialkarzinomen
US6682902B2 (en) 1999-12-16 2004-01-27 Schering Aktiengesellschaft DNA encoding a novel RG1 polypeptide
WO2001080865A2 (en) 2000-04-25 2001-11-01 Osiris Therapeutics, Inc. Joint repair using mesenchymal stem cells
NL1017973C2 (nl) * 2000-05-10 2002-11-08 Tristem Trading Cyprus Ltd Inrichting.
US6828145B2 (en) 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
EP1167517A1 (de) * 2000-06-22 2002-01-02 IsoTis N.V. Gewebetechnologie
CA2413916C (en) * 2000-06-28 2007-09-18 Ascendia Ab Dna/rna-embedding medium and method of use
US20060088512A1 (en) * 2001-10-15 2006-04-27 Monash University Treatment of T cell disorders
US7311905B2 (en) 2002-02-13 2007-12-25 Anthrogenesis Corporation Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
MXPA03005014A (es) 2000-12-06 2004-09-10 Robert J Hariri Metodo para recolectar celulas madres de la placenta.
AU2002251935B2 (en) 2001-02-14 2007-11-29 Celularity Inc. Post-partum mammalian placenta, its use and placental stem cells therefrom
WO2002081620A1 (en) * 2001-04-03 2002-10-17 Kabushiki Kaisya Advance Three-dimensional cell culture systems
JP2002315567A (ja) * 2001-04-18 2002-10-29 Yasuhiko Tabata 細胞接着活性物質を含有してなる幹細胞培養用基材
US7129034B2 (en) * 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
CA2470853A1 (en) * 2001-12-13 2003-06-19 Japan Science And Technology Agency Human cell culture medium and culture method
US20040076622A1 (en) * 2002-03-02 2004-04-22 Board Of Regents, The University Of Texas System Local production and/or delivery of anti-cancer agents by stromal cell precursors
AU2003222043A1 (en) * 2002-03-18 2003-10-08 National Jewish Medical And Research Center Method for production of neutrophils and uses therefor
US7498171B2 (en) * 2002-04-12 2009-03-03 Anthrogenesis Corporation Modulation of stem and progenitor cell differentiation, assays, and uses thereof
CA2487932A1 (en) * 2002-05-30 2003-12-11 Macrogenics, Inc. Cd16a binding proteins and use for the treatment of immune disorders
US7838290B2 (en) * 2002-07-25 2010-11-23 The Scripps Research Institute Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith
AU2003298775B2 (en) 2002-11-26 2008-07-17 Anthrogenesis Corporation Cytotherapeutics, cytotherapeutic units and methods for treatments using them
NZ566132A (en) * 2003-02-13 2009-09-25 Anthrogenesis Corp Use of umbilical cord blood to treat inflammation, ParkinsonÆs disease or diabetes
US20050003533A1 (en) * 2003-05-08 2005-01-06 Pawel Kalinski Mature type-1 polarized dendritic cells with enhanced IL-12 production and methods of serum-free production and use
US20050023085A1 (en) * 2003-07-30 2005-02-03 Munton Timothy John Lifesaver apparatus
US20080279812A1 (en) * 2003-12-05 2008-11-13 Norwood Immunology, Ltd. Disease Prevention and Vaccination Prior to Thymic Reactivation
DE602004028513D1 (de) 2003-12-23 2010-09-16 Mount Sinai Hospital Corp Verfahren zum nachweis von mit endometrialer krankheit oder phase assoziierten markern
US20070037276A1 (en) * 2004-02-23 2007-02-15 Eudes Francois Marie De Crecy Continuous culture apparatus with mobile vessel, allowing selection of fitter cell variants and producing a culture in a continuous manner
CA2566561C (en) * 2004-06-04 2013-01-08 Universite Pierre Et Marie Curie - Paris Vi A method for producing red blood cells
CN1993460A (zh) * 2004-07-12 2007-07-04 索林集团意大利有限公司 用于培养人细胞的装置和方法
US20060035375A1 (en) * 2004-08-16 2006-02-16 Head Jonathan F Method for selectively culturing epithelial or carcinoma cells
US7989001B2 (en) * 2004-09-13 2011-08-02 Untae Kim Method of separating tumor cells with and without lymphotropic metastatic potential in a human carcinoma
ES2294650T3 (es) 2004-09-30 2008-04-01 Reneuron Limited Linea celular.
WO2006052960A2 (en) * 2004-11-08 2006-05-18 The General Hospital Corporation METHODS AND COMPOSITIONS FOR INCREASING STEM CELL HOMING USING Gαs ACTIVATORS
EP1877547B1 (de) 2005-05-04 2012-08-08 Commonwealth Scientific and Industrial Research Organisation Selektion, kultivierung und erzeugung auf eine zellinie festgelegter hämatopoetischer stammzellen
EP1957633B1 (de) 2005-10-13 2013-12-18 Anthrogenesis Corporation Immunmodulation unter verwendung von plazentastammzellen
US8883507B2 (en) 2005-10-18 2014-11-11 The Regents Of The University Of Colorado Conditionally immortalized long-term hematopoietic stem cells and methods of making and using such cells
CN101563097A (zh) * 2005-11-14 2009-10-21 企业合伙人风险资本公司 用于组织损伤的干细胞生长因子疗法
US8846393B2 (en) 2005-11-29 2014-09-30 Gamida-Cell Ltd. Methods of improving stem cell homing and engraftment
KR20180105266A (ko) 2005-12-29 2018-09-27 안트로제네시스 코포레이션 태반 줄기 세포 집단
AU2006332679A1 (en) 2005-12-29 2007-07-12 Anthrogenesis Corporation Co-culture of placental stem cells and stem cells from a second source
US7682822B2 (en) 2006-03-31 2010-03-23 Aastrom Biosciences, Inc. Ex vivo generated tissue system
US20080064098A1 (en) * 2006-06-05 2008-03-13 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of maternal placental cells
US20080050814A1 (en) * 2006-06-05 2008-02-28 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
CA2655990A1 (en) * 2006-06-22 2007-12-27 Tai June Yoo Restoration of hearing loss
EP2054500B1 (de) 2006-08-18 2016-11-23 Ajinomoto Co., Inc. L-glutaminsäure produzierendes bakterium und verfahren zur herstellung von l-glutaminsäure
WO2008031882A1 (en) * 2006-09-14 2008-03-20 Probiogen Ag Modular culture system for maintenance, differentiation and proliferation of cells
WO2008054825A2 (en) 2006-11-03 2008-05-08 Aastrom Biosciences, Inc. Mixed cell populations for tissue repair and separation technique for cell processing
CN106890342B (zh) * 2007-02-06 2022-09-23 俞泰俊 使用基因疗法治疗和预防神经变性疾病
JP2010518812A (ja) 2007-02-12 2010-06-03 アンスロジェネシス コーポレーション 接着性胎盤幹細胞由来の肝細胞および軟骨細胞、ならびにcd34+、cd45−胎盤幹細胞の濃縮細胞集団
CN103356710A (zh) 2007-02-12 2013-10-23 人类起源公司 利用胎盘干细胞治疗炎性疾病
WO2008121461A2 (en) * 2007-02-26 2008-10-09 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Vaccine for activating helper function of cd8+ tcells
EP2129764B1 (de) 2007-03-05 2018-10-10 Terumo BCT, Inc. Zellexpansionssystem und anwendungsverfahren
US20080220522A1 (en) * 2007-03-05 2008-09-11 Gambro Bct, Inc. Methods to Control Cell Movement in Hollow Fiber Bioreactors
WO2008124229A2 (en) * 2007-04-06 2008-10-16 Caridianbct, Inc. Improved bioreactor surfaces
WO2008128165A2 (en) * 2007-04-13 2008-10-23 Caridianbct, Inc. Cell expansion system and methods of use
EP2203176B1 (de) 2007-09-28 2014-11-26 Anthrogenesis Corporation Tumorsuppression mit menschlichem plazentaperfusat und menschlichen natürlichen killerzellen eines zwischenproduktes aus der plazenta
EP2209810A1 (de) * 2007-10-15 2010-07-28 King's College London Virales therakpeutikum
EP2218716B1 (de) * 2007-12-06 2016-11-16 Nissan Chemical Industries, Ltd. Verfahren zur amplifizierung hämatopoietischer stammzellen mit einer heterocyclischen verbindung
US10059923B2 (en) 2008-01-30 2018-08-28 Memorial Sloan Kettering Cancer Center Methods for off-the-shelf tumor immunotherapy using allogeneic T-cell precursors
US10350243B2 (en) * 2008-01-30 2019-07-16 Memorial Sloan-Kettering Cancer Center Methods for off-the-shelf-tumor immunotherapy using allogeneic T-cell precursors
CA2618163A1 (en) 2008-02-07 2009-08-07 K. W. Michael Siu Head and neck cancer biomarkers
SG10201803982TA (en) 2008-05-16 2018-07-30 Taiga Biotechnologies Inc Antibodies and processes for preparing the same
NZ591292A (en) 2008-08-20 2012-10-26 Anthrogenesis Corp Improved cell composition and methods of making the same
CA2734237C (en) 2008-08-20 2019-07-02 Anthrogenesis Corporation Treatment of stroke using isolated placental cells
SI2331109T1 (sl) 2008-08-22 2013-10-30 Anthrogenesis Corporation Postopki in sestavki za zdravljenje okvar kosti s populacijami placentarnih celic
US8784825B2 (en) 2008-08-28 2014-07-22 Taiga Biotechnologies, Inc. Modulators of MYC, methods of using the same, and methods of identifying agents that modulate MYC
EP3031907B1 (de) * 2008-11-06 2021-01-06 Indiana University Research and Technology Corporation Materialien und verfahren, zum verbessern der hämatopoetischen stammzellen-engraftment prozedur
WO2010059828A1 (en) 2008-11-19 2010-05-27 Anthrogenesis Corporation Amnion derived adherent cells
CN102448949B (zh) 2009-06-04 2014-09-03 日产化学工业株式会社 杂环化合物及造血干细胞的扩增剂
JP2011045269A (ja) * 2009-08-26 2011-03-10 Hirosaki Univ 幹細胞および/または前駆細胞の培養方法
CA2787992A1 (en) 2010-01-26 2011-08-04 Anthrogenesis Corporation Treatment of bone-related cancers using placental stem cells
PL2556145T3 (pl) 2010-04-07 2017-02-28 Anthrogenesis Corporation Angiogeneza z zastosowaniem łożyskowych komórek macierzystych
KR20130092394A (ko) 2010-04-08 2013-08-20 안트로제네시스 코포레이션 태반 줄기 세포를 사용한 사르코이드증의 치료
JP6039547B2 (ja) 2010-05-05 2016-12-07 テルモ ビーシーティー、インコーポレーテッド 中空糸型バイオリアクターシステムにおいて成長させた接着細胞を再播種する方法
MX342995B (es) 2010-07-13 2016-10-21 Anthrogenesis Corp Métodos de generar células asesinas naturales.
EP2606165B1 (de) 2010-08-16 2016-10-12 Sinai Health System Marker für den männlichen urogenitaltrakt
US9725689B2 (en) 2010-10-08 2017-08-08 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US8969315B2 (en) 2010-12-31 2015-03-03 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
DK2714059T3 (en) 2011-06-01 2019-01-21 Celularity Inc TREATMENT OF PAIN WHEN USING PLACENT STAM CELLS
WO2013055476A1 (en) 2011-09-09 2013-04-18 Anthrogenesis Corporation Treatment of amyotrophic lateral sclerosis using placental stem cells
CA2850484C (en) 2011-09-30 2021-01-26 Bluebird Bio, Inc. Compounds for improved viral transduction
WO2013121426A1 (en) 2012-02-13 2013-08-22 Gamida-Cell Ltd. Culturing of mesenchymal stem cells
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
BR112014028094B1 (pt) 2012-05-09 2022-03-03 Cantex Pharmaceuticals, Inc Uso de um heparinoide interagindo com fator de plaqueta 4
WO2014011901A2 (en) * 2012-07-11 2014-01-16 Sangamo Biosciences, Inc. Methods and compositions for delivery of biologics
JP6285930B2 (ja) 2012-07-20 2018-02-28 タイガ バイオテクノロジーズ,インク. 造血コンパートメントの再構築及び自家再構築の増進
US9567569B2 (en) 2012-07-23 2017-02-14 Gamida Cell Ltd. Methods of culturing and expanding mesenchymal stem cells
US9175266B2 (en) 2012-07-23 2015-11-03 Gamida Cell Ltd. Enhancement of natural killer (NK) cell proliferation and activity
US9175259B2 (en) 2012-08-20 2015-11-03 Terumo Bct, Inc. Method of loading and distributing cells in a bioreactor of a cell expansion system
EP3622960A1 (de) 2013-02-05 2020-03-18 Celularity, Inc. Natürliche killerzellen aus plazenta
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
EP3068867B1 (de) 2013-11-16 2018-04-18 Terumo BCT, Inc. Zellenexpansion in einem bioreaktor
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
US10077421B2 (en) 2014-04-24 2018-09-18 Terumo Bct, Inc. Measuring flow rate
EP3198006B1 (de) 2014-09-26 2021-03-24 Terumo BCT, Inc. Geplante fütterung
US20160243168A1 (en) * 2015-02-17 2016-08-25 Cantex Pharmaceuticals, Inc. Adoptive cell transfer methods
EP3258941A4 (de) 2015-02-17 2018-09-26 Cantex Pharmaceuticals, Inc. Behandlung von durch cxcl12-cxcr4 interaktion privilegierten krebs- und hämatopoetischen stammzellerkrankungen
CN107532990B (zh) * 2015-05-12 2021-11-12 芯片生物技术株式会社 单一粒子解析方法及用于该解析的系统
GB201508752D0 (en) 2015-05-21 2015-07-01 Mason Christopher And Veraitch Farlan S Cell culture device, system and methods of use thereof
AU2016281662B2 (en) * 2015-06-23 2021-12-02 Board Of Regents Of The University Of Texas System Methods and apparatus for conditioning cell populations for cell therapies
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
CA2996582A1 (en) 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
JPWO2017061528A1 (ja) * 2015-10-09 2018-07-26 国立大学法人名古屋大学 血小板製造用デバイス、血小板製造装置及び血小板製造方法
CN108883136A (zh) 2016-02-12 2018-11-23 蓝鸟生物公司 Vcn增强子组合物及其使用方法
KR20250134722A (ko) 2016-02-12 2025-09-11 블루버드 바이오, 인코포레이티드. Vcn 인핸서 조성물 및 이의 사용 방법
JP6965874B2 (ja) * 2016-03-28 2021-11-10 ソニーグループ株式会社 細胞培養方法
KR102170373B1 (ko) 2016-05-02 2020-10-27 인테그리컬쳐 인코포레이티드 성장 유도 시스템, 성장 유도 제어 장치, 성장 유도 제어 방법, 및 성장 유도 제어 프로그램
WO2017205667A1 (en) 2016-05-25 2017-11-30 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US10583156B2 (en) 2016-12-02 2020-03-10 Taiga Biotechnologies, Inc. Nanoparticle formulations
JP7393945B2 (ja) 2017-03-31 2023-12-07 テルモ ビーシーティー、インコーポレーテッド 細胞増殖
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
WO2019191545A1 (en) * 2018-03-30 2019-10-03 Dsm Ip Assets B.V. Method of reducing emulsion by broth washing
EP3952909A4 (de) 2019-04-08 2023-06-07 Taiga Biotechnologies, Inc. Zusammensetzungen und verfahren zur kryokonservierung von immunzellen
SG11202112529WA (en) 2019-05-14 2021-12-30 Taiga Biotechnologies Inc Compositions and methods for treating t cell exhaustion
US20210008161A1 (en) 2019-06-17 2021-01-14 Crispr Therapeutics Ag Methods and compositions for improved homology directed repair

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4481946A (en) * 1980-08-14 1984-11-13 Altshuler John H Bone marrow transplant method and apparatus
US4486188A (en) * 1980-08-14 1984-12-04 Applied Medical Devices, Inc. Bone marrow transplant method and apparatus
US4514499A (en) * 1983-02-04 1985-04-30 Corning Glass Works Cell culture using a monolithic support
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5032508A (en) * 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5032407A (en) * 1987-01-16 1991-07-16 Ohio University Edison Animal Biotechnology Center Gene transfer using transformed, neodetermined, embryonic cells
US5399493A (en) * 1989-06-15 1995-03-21 The Regents Of The University Of Michigan Methods and compositions for the optimization of human hematopoietic progenitor cell cultures
KR100196062B1 (ko) * 1989-06-15 1999-06-15 로버트 에프. 게이민 세포를 성장시키기 위한 방법, 조성물 및 장치
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5199942A (en) * 1991-06-07 1993-04-06 Immunex Corporation Method for improving autologous transplantation
NL9101680A (nl) * 1991-10-04 1993-05-03 Tno Werkwijze voor het genetisch modificeren van beenmergcellen van primaten, alsmede daarbij bruikbare cellen die recombinante retrovirale vectoren produceren.
CA2131368A1 (en) * 1992-03-04 1993-09-16 Chu-Chih Shih Culturing of hematopoietic stem cells and their genetic engineering
WO1993018136A1 (en) * 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
EP0716570A4 (de) * 1993-09-03 1997-12-17 Systemix Inc Genetisch modifizierte menschliche hämatopoietische stammzellen und ihre nachkommen

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KR930703434A (ko) 1993-11-30
EP0575350A1 (de) 1993-12-29
EP1473360A2 (de) 2004-11-03
EP0575350A4 (en) 1997-07-02
AU5059296A (en) 1996-07-11
WO1992011355A1 (en) 1992-07-09
AU687674B2 (en) 1998-02-26
US5670351A (en) 1997-09-23
JPH06505151A (ja) 1994-06-16
AU9175091A (en) 1992-07-22
JP2000189157A (ja) 2000-07-11
EP1473360A3 (de) 2005-02-02
KR100225307B1 (ko) 1999-10-15
EP0575350B1 (de) 2005-05-11
CA2100268A1 (en) 1992-06-18
JPH11221074A (ja) 1999-08-17
US5399493A (en) 1995-03-21
JP2001120261A (ja) 2001-05-08
AU665525B2 (en) 1996-01-11
EP1473360A8 (de) 2010-07-28
JP2006158401A (ja) 2006-06-22
DE69133459T2 (de) 2006-01-19
CA2100268C (en) 2008-12-02
DE69133459D1 (de) 2005-06-16

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