ATE320495T1 - Kalziumfreie subtilisin mutanten - Google Patents

Kalziumfreie subtilisin mutanten

Info

Publication number
ATE320495T1
ATE320495T1 AT95918373T AT95918373T ATE320495T1 AT E320495 T1 ATE320495 T1 AT E320495T1 AT 95918373 T AT95918373 T AT 95918373T AT 95918373 T AT95918373 T AT 95918373T AT E320495 T1 ATE320495 T1 AT E320495T1
Authority
AT
Austria
Prior art keywords
subtilisin mutants
calcium
free subtilisin
mutants
free
Prior art date
Application number
AT95918373T
Other languages
English (en)
Inventor
Philip N Bryan
Patrick A Alexander
Susan L Strausberg
Original Assignee
Univ Maryland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland filed Critical Univ Maryland
Application granted granted Critical
Publication of ATE320495T1 publication Critical patent/ATE320495T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Auxiliary Methods And Devices For Loading And Unloading (AREA)
  • Cosmetics (AREA)
AT95918373T 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten ATE320495T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/309,069 US5567601A (en) 1993-06-01 1994-09-20 Subtilisin mutants lacking a primary calcium binding site

Publications (1)

Publication Number Publication Date
ATE320495T1 true ATE320495T1 (de) 2006-04-15

Family

ID=23196540

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95918373T ATE320495T1 (de) 1994-09-20 1995-04-28 Kalziumfreie subtilisin mutanten

Country Status (12)

Country Link
US (1) US5567601A (de)
EP (1) EP0804592B1 (de)
JP (1) JPH10506277A (de)
KR (1) KR100364448B1 (de)
CN (1) CN1168829C (de)
AT (1) ATE320495T1 (de)
AU (1) AU693552B2 (de)
BR (1) BR9508981A (de)
CA (1) CA2200563C (de)
DE (1) DE69534875T2 (de)
NZ (1) NZ285286A (de)
WO (1) WO1996009396A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
AU1225099A (en) * 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
EP1082443A1 (de) * 1998-03-26 2001-03-14 The Procter & Gamble Company Serinproteasevarianten mit deletierten und substituierten aminosäuren
BR9909130A (pt) * 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
US6908757B1 (en) * 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
WO2001007578A2 (en) * 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
JP2003505067A (ja) * 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
EP1321513A1 (de) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variante mit verbesserten Eigenschaften
JP5328002B2 (ja) * 2002-01-16 2013-10-30 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
JP4908196B2 (ja) * 2003-03-21 2012-04-04 ノボザイムス アクティーゼルスカブ サブチラーゼ
EP1651751B1 (de) * 2003-08-06 2015-11-18 University Of Maryland Biotechnology Institute Gentechnisch hergestellte proteasen zur affinitätsreinigung und prozessierung von fusionsproteinen
EP3204401A1 (de) * 2014-10-10 2017-08-16 Enzypep B.V. Peptidfragmentkondensation und cyclisierung unter verwendung einer subtilisin-variante mit verbessertem synthese-hydrolyse-verhältnis
GB201803654D0 (en) * 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
PL3704143T3 (pl) * 2018-03-09 2024-04-15 Fresenius Kabi Ipsum S.R.L. Chemo-enzymatyczna synteza liraglutydu, semaglutydu i glp-1
WO2025262181A1 (en) * 2024-06-19 2025-12-26 Fresenius Kabi Ipsum S.R.L. Method to improve the producibility of a peptiligase enzyme

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4908773A (en) * 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
ATE371029T1 (de) * 1987-04-06 2007-09-15 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins
DE69034159T2 (de) * 1989-06-26 2005-02-17 Unilever N.V. Enzymatische Waschmittelzusammensetzungen
BR9006832A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicao detergente enzimatica
DE69128094T2 (de) * 1990-12-21 1998-03-19 Novonordisk As Enzym-mutanten mit einer geringen variation der molekularladung über einen ph-bereich
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
KR950702633A (ko) * 1992-07-17 1995-07-29 한스 발터 라벤 고알칼리성 세린 프로테아제(high alkaline serine proteases)
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants

Also Published As

Publication number Publication date
DE69534875T2 (de) 2006-11-16
CN1168829C (zh) 2004-09-29
BR9508981A (pt) 1997-12-30
JPH10506277A (ja) 1998-06-23
MX9702038A (es) 1997-10-31
CA2200563C (en) 2005-01-04
CA2200563A1 (en) 1996-03-28
EP0804592B1 (de) 2006-03-15
NZ285286A (en) 1998-06-26
US5567601A (en) 1996-10-22
DE69534875D1 (de) 2006-05-11
KR100364448B1 (ko) 2003-01-24
AU2433595A (en) 1996-04-09
EP0804592A1 (de) 1997-11-05
CN1166183A (zh) 1997-11-26
WO1996009396A1 (en) 1996-03-28
AU693552B2 (en) 1998-07-02
HK1004150A1 (en) 1998-11-20

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