ATE324376T1 - Reagenz zur isolierung von rna - Google Patents
Reagenz zur isolierung von rnaInfo
- Publication number
- ATE324376T1 ATE324376T1 AT02701972T AT02701972T ATE324376T1 AT E324376 T1 ATE324376 T1 AT E324376T1 AT 02701972 T AT02701972 T AT 02701972T AT 02701972 T AT02701972 T AT 02701972T AT E324376 T1 ATE324376 T1 AT E324376T1
- Authority
- AT
- Austria
- Prior art keywords
- rna
- present
- methods
- reagents
- kits
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 238000002123 RNA extraction Methods 0.000 abstract 1
- 229920002472 Starch Polymers 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 abstract 1
- 108091092330 cytoplasmic RNA Proteins 0.000 abstract 1
- -1 for example Proteins 0.000 abstract 1
- 238000001502 gel electrophoresis Methods 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 235000013824 polyphenols Nutrition 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
- 235000018553 tannin Nutrition 0.000 abstract 1
- 229920001864 tannin Polymers 0.000 abstract 1
- 239000001648 tannin Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26125601P | 2001-01-16 | 2001-01-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE324376T1 true ATE324376T1 (de) | 2006-05-15 |
Family
ID=22992514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02701972T ATE324376T1 (de) | 2001-01-16 | 2002-01-16 | Reagenz zur isolierung von rna |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6875857B2 (de) |
| EP (1) | EP1399459B1 (de) |
| JP (1) | JP2004523229A (de) |
| AT (1) | ATE324376T1 (de) |
| CA (1) | CA2435241A1 (de) |
| DE (1) | DE60210963T2 (de) |
| WO (1) | WO2002057289A1 (de) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE324376T1 (de) * | 2001-01-16 | 2006-05-15 | Invitrogen Corp | Reagenz zur isolierung von rna |
| EP1476573B1 (de) | 2002-01-28 | 2010-01-06 | Ambion, Inc. | Für den nachweis von nukleinsäuren kompetente biologische rohderivate |
| AU2003245465A1 (en) * | 2002-02-13 | 2003-09-04 | Medimolecular Pty. Ltd. | Method of isolating nucleic acids from stool samples |
| GB0217963D0 (en) * | 2002-08-02 | 2002-09-11 | Cyclops Genome Sciences Ltd | Purification of nucleic acids |
| US20060134617A1 (en) * | 2002-12-18 | 2006-06-22 | University Of Geneva | Enzymatic method for the isolation of dna from plant tissue |
| US7267950B2 (en) | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
| US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
| US20070015165A1 (en) * | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| US20070072229A1 (en) * | 2005-09-28 | 2007-03-29 | Pierce Milwaukee, L.L.C. | Method for isolation, amplification and quantitation of ribonucleic acid |
| US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
| US20070202511A1 (en) * | 2006-02-28 | 2007-08-30 | Sigma-Aldrich Co. | Methods and compositions for the rapid isolation of small RNA molecules |
| EP2004822B1 (de) * | 2006-03-30 | 2013-06-12 | Council of Scientific and Industrial Research | Schnelles rna-isolierungsverfahren und kit dafür |
| US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
| US8357672B2 (en) * | 2008-06-13 | 2013-01-22 | Bio-Rad Laboratories, Inc. | Cell lysis reagent for isolation of RNA |
| EP2955233A1 (de) | 2008-12-19 | 2015-12-16 | Life Technologies Corporation | Proteinase K Inhibitore, Verfahren und Zusammensetzungen davon |
| CN102250876B (zh) | 2011-05-18 | 2014-08-13 | 李学敬 | 从生物材料中分离纯化rna的方法 |
| CN102690806A (zh) * | 2012-05-02 | 2012-09-26 | 江苏大学 | 一种简单、快速提取柳枝稷组织总rna的方法 |
| CN103571824B (zh) | 2012-08-09 | 2016-04-13 | 财团法人工业技术研究院 | 用于分离核酸的组合物及方法 |
| CN103952399A (zh) * | 2014-04-18 | 2014-07-30 | 天根生化科技(北京)有限公司 | 一种使用含有指示剂的酚胍盐裂解液提取核糖核酸的方法 |
| CN104031908A (zh) * | 2014-06-17 | 2014-09-10 | 中国林业科学研究院林业研究所 | 一种植物组织总rna提取方法 |
| FR3043695B1 (fr) | 2015-11-17 | 2019-10-25 | ISP Investments LLC. | Procede d'obtention d'un extrait aqueux enrichi en petits arn a partir d'une matiere vegetal et extraits issus du procede |
| WO2018037281A1 (en) | 2016-08-22 | 2018-03-01 | Biolumic Limited | System, device and methods of seed treatment |
| EP4234710A3 (de) | 2017-03-28 | 2023-11-01 | Phase Scientific International, Ltd. | Verfahren zur genauen diagnose von gegen krankheiten gerichtete biomarkers in der flüssigbiopsie |
| SG11201909324WA (en) | 2017-06-01 | 2019-11-28 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
| AU2018293468A1 (en) | 2017-06-29 | 2020-01-30 | Biolumic Limited | Method to improve crop yield and/or quality |
| WO2019038594A2 (en) | 2017-08-21 | 2019-02-28 | Biolumic Limited | TRANSGENIC PLANTS WITH HIGH GROWTH AND HIGH RUSTICITY |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| ES2964100T3 (es) | 2017-09-18 | 2024-04-04 | Phase Scient International Ltd | Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos |
| EP3740588A4 (de) | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | Verfahren zur isolierung und reinigung von nukleinsäuren unter verwendung eines fest-flüssig-phasensystems |
| WO2019143895A1 (en) | 2018-01-19 | 2019-07-25 | Yin To Chiu | Spontaneous nucleic acid purification and concentration in a single step |
| CN110616218B (zh) * | 2019-11-12 | 2023-06-13 | 苏州森苗生物科技有限公司 | 血液rna保存液及其制备方法、血液rna保存管、保存提取方法 |
| EP4121530A1 (de) | 2020-03-20 | 2023-01-25 | Phase Scientific International, Ltd. | Zusammensetzungen und verfahren zur ribonukleinsäureextraktion |
| CN111647595A (zh) * | 2020-06-23 | 2020-09-11 | 青岛农业大学 | 一种耐冬山茶叶片总rna提取方法 |
| CZ309658B6 (cs) | 2021-02-08 | 2023-06-21 | Bioinova, A.S. | Roztok pro molekulární diagnostiku virových infekcí pomocí PCR a odběrová sada |
| CN115109776A (zh) * | 2022-07-21 | 2022-09-27 | 山东省农业科学院 | 一种从芋头块茎组织中提取总rna的提取液及其提取方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61132189A (ja) | 1984-12-03 | 1986-06-19 | Norin Suisansyo Shokuhin Sogo Kenkyusho | ダイズグリシニンcDNA |
| US4833239A (en) | 1987-01-29 | 1989-05-23 | Genetics Institute, Inc. | Method for the isolation and purification of DNA molecules |
| US4843155A (en) | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| US5585264A (en) * | 1988-07-15 | 1996-12-17 | University Of Saskatchewan | Nucleotide sequences encoding recombinant bovine herpesvirus type-1 GI, GIII and GIV polypeptides |
| US5098603A (en) | 1990-01-16 | 1992-03-24 | Eastman Kodak Company | Stabilized phenol solution |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| BR9506270A (pt) | 1994-06-17 | 1997-08-12 | Kirin Brewery | Receptor de elicitador de glucano e molécula de dna codificado para o mesmo |
| AUPN283495A0 (en) | 1995-05-05 | 1995-06-01 | Australian National University, The | Plant promoter activated by fungal infection |
| DE69734263T2 (de) | 1996-07-12 | 2006-07-13 | Toyo Boseki K.K. | Verfahren zur Isolierung von Ribonucleinsäuren. |
| JP2001524820A (ja) * | 1997-04-10 | 2001-12-04 | ライフ テクノロジーズ,インコーポレイテッド | Rna単離試薬および方法 |
| DE19916534A1 (de) | 1999-04-13 | 2000-10-19 | Gsf Forschungszentrum Umwelt | RNA-Isolierungs-Kit |
| ATE324376T1 (de) * | 2001-01-16 | 2006-05-15 | Invitrogen Corp | Reagenz zur isolierung von rna |
| WO2002065125A1 (en) * | 2001-02-13 | 2002-08-22 | Invitrogen Corporation | Methods and compositions for isolation of biological macromolecules |
-
2002
- 2002-01-16 AT AT02701972T patent/ATE324376T1/de not_active IP Right Cessation
- 2002-01-16 CA CA002435241A patent/CA2435241A1/en not_active Abandoned
- 2002-01-16 JP JP2002557965A patent/JP2004523229A/ja active Pending
- 2002-01-16 EP EP02701972A patent/EP1399459B1/de not_active Expired - Lifetime
- 2002-01-16 US US10/046,667 patent/US6875857B2/en not_active Expired - Lifetime
- 2002-01-16 WO PCT/US2002/001017 patent/WO2002057289A1/en not_active Ceased
- 2002-01-16 DE DE60210963T patent/DE60210963T2/de not_active Expired - Fee Related
-
2005
- 2005-01-10 US US11/030,903 patent/US20050158783A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050158783A1 (en) | 2005-07-21 |
| JP2004523229A (ja) | 2004-08-05 |
| WO2002057289A1 (en) | 2002-07-25 |
| US6875857B2 (en) | 2005-04-05 |
| EP1399459A1 (de) | 2004-03-24 |
| DE60210963D1 (de) | 2006-06-01 |
| WO2002057289A8 (en) | 2003-04-17 |
| EP1399459A4 (de) | 2004-09-08 |
| EP1399459B1 (de) | 2006-04-26 |
| US20030078412A1 (en) | 2003-04-24 |
| CA2435241A1 (en) | 2002-07-25 |
| DE60210963T2 (de) | 2006-11-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |