ATE333469T1 - Verfahren zur reinigung hochanionischer proteine - Google Patents

Verfahren zur reinigung hochanionischer proteine

Info

Publication number
ATE333469T1
ATE333469T1 AT02749564T AT02749564T ATE333469T1 AT E333469 T1 ATE333469 T1 AT E333469T1 AT 02749564 T AT02749564 T AT 02749564T AT 02749564 T AT02749564 T AT 02749564T AT E333469 T1 ATE333469 T1 AT E333469T1
Authority
AT
Austria
Prior art keywords
chanionic
proteins
containing molecules
mixture
purifying highly
Prior art date
Application number
AT02749564T
Other languages
English (en)
Inventor
Jonathan L Coffman
William B Foster
Shujun Sun
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE333469T1 publication Critical patent/ATE333469T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02749564T 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine ATE333469T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05

Publications (1)

Publication Number Publication Date
ATE333469T1 true ATE333469T1 (de) 2006-08-15

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine

Country Status (11)

Country Link
US (1) US7223848B2 (de)
EP (2) EP1404428B1 (de)
JP (1) JP4288153B2 (de)
AT (2) ATE421536T1 (de)
AU (1) AU2008203302B2 (de)
CA (1) CA2448788C (de)
DE (2) DE60213248T2 (de)
DK (2) DK1404428T3 (de)
ES (2) ES2266535T3 (de)
NZ (1) NZ530025A (de)
WO (1) WO2002098531A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
EP1963367A4 (de) * 2005-12-06 2009-05-13 Amgen Inc Reinigungsschritte in mehrstufigen proteinreinigungsverfahren
WO2007081906A2 (en) * 2006-01-06 2007-07-19 Amgen Inc. Purification by column-chromatography using eluants containing organic solvents
TW200821026A (en) * 2006-09-08 2008-05-16 Wyeth Corp Arginine wash in protein purification using affinity chromatography
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
DK2089429T3 (en) * 2006-10-19 2015-01-12 Janssen Biotech Inc PROCEDURE FOR MANUFACTURING NON-AGGREGATED ANTIBODY-FC DOMAINS
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
CA2932207A1 (en) * 2008-10-20 2010-12-09 Abbvie Inc. Isolation and purification of antibodies using protein a affinity chromatography
US20120149875A1 (en) * 2009-01-12 2012-06-14 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
BR112012014937B1 (pt) 2009-12-18 2021-01-19 Novartis Ag método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade
KR101953405B1 (ko) 2011-06-01 2019-02-28 노파르티스 아게 친화성 크로마토그래피를 위한 세척 용액 및 방법
WO2021147857A1 (en) * 2020-01-20 2021-07-29 Wuxi Biologics (Shanghai) Co., Ltd A novel wash buffer solution for affinity chromatography

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US5843707A (en) 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
EP1104809A1 (de) 1994-04-09 2001-06-06 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von Alpha-Interferon
US6008328A (en) 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
AU729459B2 (en) 1996-11-15 2001-02-01 Genentech Inc. Purification of neurotrophins
ES2293661T3 (es) * 1996-11-27 2008-03-16 Genentech, Inc. Purificacion por afinidad de polipeptido en una matriz de proteinas a.
DE69930653T2 (de) 1998-09-24 2007-01-25 Pharming Intellectual Property Bv Reinigung von fibrinogen aus milch durch den einsatz von kationenaustauschchromatographie
CN1342085A (zh) * 1998-10-30 2002-03-27 遗传研究所有限公司 通过p－选择素配体(psgl)拮抗剂抑制细胞素t细胞的分化
JP2003517580A (ja) 1999-01-21 2003-05-27 メタモーフイクス・インコーポレーテツド 増殖分化因子インヒビター及びそれらの用途
EP1681299B1 (de) * 2000-03-27 2009-11-11 Genetics Institute, LLC Verfahren zur Reinigung stark anionischer Proteine

Also Published As

Publication number Publication date
US7223848B2 (en) 2007-05-29
EP1404428B1 (de) 2006-07-19
NZ530025A (en) 2005-06-24
ES2321539T3 (es) 2009-06-08
EP1404428A1 (de) 2004-04-07
CA2448788A1 (en) 2002-12-12
AU2008203302B2 (en) 2011-10-27
DE60213248D1 (de) 2006-08-31
EP1714982A3 (de) 2006-12-20
JP4288153B2 (ja) 2009-07-01
ES2266535T3 (es) 2007-03-01
DE60231016D1 (de) 2009-03-12
ATE421536T1 (de) 2009-02-15
EP1714982A2 (de) 2006-10-25
WO2002098531A1 (en) 2002-12-12
AU2008203302A1 (en) 2008-08-14
EP1714982B1 (de) 2009-01-21
US20030050450A1 (en) 2003-03-13
DK1404428T3 (da) 2006-10-30
EP1404428A4 (de) 2004-12-08
DE60213248T2 (de) 2006-11-30
CA2448788C (en) 2010-08-24
JP2004537042A (ja) 2004-12-09
DK1714982T3 (da) 2009-05-04

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