ATE488582T1 - Verfahren zur reinigung von faktor ix - Google Patents

Verfahren zur reinigung von faktor ix

Info

Publication number
ATE488582T1
ATE488582T1 AT05012614T AT05012614T ATE488582T1 AT E488582 T1 ATE488582 T1 AT E488582T1 AT 05012614 T AT05012614 T AT 05012614T AT 05012614 T AT05012614 T AT 05012614T AT E488582 T1 ATE488582 T1 AT E488582T1
Authority
AT
Austria
Prior art keywords
purifying factor
factor
hydroxyapatite
resins
purification
Prior art date
Application number
AT05012614T
Other languages
English (en)
Inventor
Barry W Foster
Robert J Costigan
Duane Bonam
Mary B Switzer
Rochelle Walsh
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE488582T1 publication Critical patent/ATE488582T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT05012614T 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix ATE488582T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/472,823 US5714583A (en) 1995-06-07 1995-06-07 Factor IX purification methods

Publications (1)

Publication Number Publication Date
ATE488582T1 true ATE488582T1 (de) 2010-12-15

Family

ID=23877079

Family Applications (2)

Application Number Title Priority Date Filing Date
AT96915892T ATE393217T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix
AT05012614T ATE488582T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT96915892T ATE393217T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix

Country Status (11)

Country Link
US (2) US5714583A (de)
EP (2) EP0832200B1 (de)
JP (1) JP3676817B2 (de)
AT (2) ATE393217T1 (de)
AU (1) AU5754396A (de)
CA (1) CA2220501C (de)
DE (2) DE69637509T2 (de)
DK (2) DK0832200T3 (de)
ES (2) ES2306450T3 (de)
PT (2) PT1571208E (de)
WO (1) WO1996040883A1 (de)

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US6150328A (en) 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
US6291206B1 (en) 1993-09-17 2001-09-18 Genetics Institute, Inc. BMP receptor proteins
AU689184B2 (en) 1993-12-07 1998-03-26 Genetics Institute, Llc BMP-12, BMP-13 and tendon-inducing compositions thereof
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
NZ507557A (en) † 1998-05-06 2003-10-31 Genentech Inc Protein purification by ion exchange chromatography
NZ511034A (en) 1998-09-21 2004-03-26 American Nat Red Cross Methods of downmodulating the immune response to therapeutic proteins
US6727224B1 (en) 1999-02-01 2004-04-27 Genetics Institute, Llc. Methods and compositions for healing and repair of articular cartilage
DK1223990T3 (da) 1999-10-15 2004-11-29 Inst Genetics Llc Formuleringer af hyaluronsyre til tilförsel af osteogene proteiner
AU2002225681A1 (en) * 2000-11-15 2002-05-27 Globe Immune, Inc. Yeast-dentritic cell vaccines and uses thereof
US7226587B2 (en) 2001-06-01 2007-06-05 Wyeth Compositions and methods for systemic administration of sequences encoding bone morphogenetic proteins
TWI267378B (en) 2001-06-08 2006-12-01 Wyeth Corp Calcium phosphate delivery vehicles for osteoinductive proteins
KR100451308B1 (ko) * 2001-12-28 2004-10-06 선바이오(주) 단백질 의약품 정제 방법에서의 바이러스 제거 방법
EP1581644B1 (de) 2003-01-09 2007-06-06 Genentech, Inc. Reinigung von polypeptiden
EP1617799A2 (de) * 2003-04-09 2006-01-25 Wyeth Hömophilie-behandlung durch inhalation von gerinnungsfaktoren
CA2571292C (en) * 2004-06-30 2013-05-21 Nektar Therapeutics Al, Corporation Polymer-factor ix moiety conjugates
AU2006331501B2 (en) 2005-12-21 2013-09-05 Aptevo Biotherapeutics Llc Method of producing biologically active vitamin K dependent proteins by recombinant methods
TWI428448B (zh) * 2006-03-24 2014-03-01 Syntonix Pharmaceuticals Inc 作為第九因子（ｆａｃｔｏｒ ｉｘ）原肽處理酶之ｐｃ５
DK2125866T3 (da) * 2007-02-28 2013-07-29 Baxter Int Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler
JP6050927B2 (ja) 2007-04-26 2016-12-21 シーエヌジェイ ホールディングス，インコーポレイテッド 高シアル酸含量を有する組換えビタミンｋ依存性タンパク質およびその調製方法
RU2353386C1 (ru) * 2007-10-11 2009-04-27 Общество с ограниченной ответственностью "БиоГениус" Способ получения концентрата ix фактора свертывания крови человека
ES2533266T5 (es) 2007-10-30 2018-04-18 Genentech, Inc. Purificación de anticuerpos mediante cromatografía de intercambio catiónico
US20110166332A1 (en) * 2008-09-12 2011-07-07 Ge Healthcare Bio-Sciences Ab Enhanced antibody aggregate removal with capto adhere in the presence of protein-excluded zwitterions
FR2946348B1 (fr) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement Procede de preparation d'une composition de complexe prothrombique a haut degre de purete
EP2451963B1 (de) 2009-07-10 2014-04-30 CSL Limited Verfahren zur erhöhung des expressionsertrags von vitamin-k-abhängigen proteinen
WO2011053738A1 (en) 2009-10-30 2011-05-05 Inspiration Biopharmaceuticals, Inc. Method of producing recombinant vitamin k dependent proteins
US8697844B2 (en) 2009-11-24 2014-04-15 Novo Nordisk A/S Method of purifying pegylated proteins
KR101951446B1 (ko) 2010-03-30 2019-02-22 옥타파마 아게 성장 인자 단백질을 정제하는 방법
WO2011121020A1 (en) * 2010-03-30 2011-10-06 Octapharma Ag A process for purifying vitamin k dependent proteins such as coagulation factor ix
SG185005A1 (en) * 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
SG191186A1 (en) 2010-12-15 2013-07-31 Baxter Int Eluate collection using conductivity gradient
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
AU2012304763A1 (en) * 2011-09-06 2014-03-06 Medimmune Llc Methods for processing coagulation factors
ES2700933T3 (es) * 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
AU2012322948B2 (en) * 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
NZ629845A (en) 2013-03-15 2016-10-28 Baxalta Inc Purification method for vitamin k dependent proteins by anion exchange chromatography
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
CN114134109B (zh) * 2021-12-10 2023-01-24 广州远想生物科技股份有限公司 Egf间充质干细胞外泌体的纯化方法

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DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
US5055557A (en) * 1983-03-04 1991-10-08 Scripps Clinic & Research Foundation Ultrapurification of factor IX and other vitamin K-dependent proteins
WO1985001941A1 (en) * 1983-10-28 1985-05-09 New England Medical Center Hospitals, Inc. Method for the purification and isolation of blood clotting proteins using conformation specific antibodies
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4831118A (en) * 1987-08-07 1989-05-16 Scripps Clinic And Research Foundation Flouroplastic immunoaffinity columns for purification of blood proteins
EP0317376B2 (de) * 1987-10-23 1996-04-03 Centre Regional De Transfusion Sanguine De Lille Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat
WO1989005652A1 (en) * 1987-12-21 1989-06-29 American National Red Cross Pure factor ix product
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
CA1330302C (en) * 1988-01-18 1994-06-21 Miroslav Rybak Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use
DE3826792C1 (de) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (de) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
AT402261B (de) * 1991-01-25 1997-03-25 Immuno Ag Komplex enthaltend den gerinnungsfaktor ix
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods

Also Published As

Publication number Publication date
US6627737B1 (en) 2003-09-30
EP1571208A1 (de) 2005-09-07
JPH11506603A (ja) 1999-06-15
CA2220501A1 (en) 1996-12-19
EP0832200B1 (de) 2008-04-23
WO1996040883A1 (en) 1996-12-19
ES2306450T3 (es) 2008-11-01
DK0832200T3 (da) 2008-07-21
DE69638291D1 (de) 2010-12-30
EP0832200A1 (de) 1998-04-01
CA2220501C (en) 2014-01-07
ATE393217T1 (de) 2008-05-15
PT832200E (pt) 2008-07-17
ES2353798T3 (es) 2011-03-07
EP1571208B1 (de) 2010-11-17
JP3676817B2 (ja) 2005-07-27
US5714583A (en) 1998-02-03
DE69637509T2 (de) 2009-05-28
AU5754396A (en) 1996-12-30
DK1571208T3 (da) 2011-01-24
DE69637509D1 (de) 2008-06-05
PT1571208E (pt) 2011-01-04

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