ATE421536T1 - Verfahren zur reinigung stark anionischer proteine - Google Patents

Verfahren zur reinigung stark anionischer proteine

Info

Publication number
ATE421536T1
ATE421536T1 AT06010184T AT06010184T ATE421536T1 AT E421536 T1 ATE421536 T1 AT E421536T1 AT 06010184 T AT06010184 T AT 06010184T AT 06010184 T AT06010184 T AT 06010184T AT E421536 T1 ATE421536 T1 AT E421536T1
Authority
AT
Austria
Prior art keywords
purifying
containing molecules
mixture
anionic proteins
strongly anionic
Prior art date
Application number
AT06010184T
Other languages
English (en)
Inventor
Jonathan L Coffmann
William B Foster
Shujun Sun
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE421536T1 publication Critical patent/ATE421536T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT06010184T 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine ATE421536T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05

Publications (1)

Publication Number Publication Date
ATE421536T1 true ATE421536T1 (de) 2009-02-15

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine

Country Status (11)

Country Link
US (1) US7223848B2 (de)
EP (2) EP1714982B1 (de)
JP (1) JP4288153B2 (de)
AT (2) ATE333469T1 (de)
AU (1) AU2008203302B2 (de)
CA (1) CA2448788C (de)
DE (2) DE60231016D1 (de)
DK (2) DK1714982T3 (de)
ES (2) ES2321539T3 (de)
NZ (1) NZ530025A (de)
WO (1) WO2002098531A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
WO2007075283A2 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
WO2007081906A2 (en) * 2006-01-06 2007-07-19 Amgen Inc. Purification by column-chromatography using eluants containing organic solvents
CN101511387A (zh) * 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2089429B1 (de) * 2006-10-19 2014-11-26 Janssen Biotech, Inc. Verfahren zur herstellung nicht aggregierter antikörper-fc-domänen
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
BRPI0920572A8 (pt) 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos
EP2389386A4 (de) * 2009-01-12 2013-11-06 Ge Healthcare Bio Sciences Ab Affinitätschromatographiematrix
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
ES2982297T3 (es) 2009-12-18 2024-10-15 Novartis Ag Método de cromatografía de afinidad
EP2714714B1 (de) 2011-06-01 2018-06-20 Novartis AG Waschlösung zur verwendung in der affinätschromatographie
EP4093745A4 (de) * 2020-01-20 2024-03-06 Wuxi Biologics Ireland Limited Neue waschpufferlösung für affinitätschromatographie

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983722A (en) * 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) * 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US6277975B1 (en) * 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5843707A (en) * 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
ATE205256T1 (de) * 1994-04-09 2001-09-15 Hoffmann La Roche Verfahren zur herstellung von alpha-interferon
US6008328A (en) * 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) * 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) * 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) * 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
AU729459B2 (en) * 1996-11-15 2001-02-01 Genentech Inc. Purification of neurotrophins
DE69738075T2 (de) * 1996-11-27 2008-05-21 Genentech, Inc., South San Francisco Affinitätsreinigung von polypeptiden an einer protein a-matrix
EP1115742B1 (de) * 1998-09-24 2006-03-29 Pharming Intellectual Property BV Reinigung von fibrinogen aus milch durch den einsatz von kationenaustauschchromatographie
CA2347119A1 (en) * 1998-10-30 2000-05-11 Genetics Institute, Inc. Inhibition of differentiation of cytotoxic t-cells by p-selectin ligand (psgl) antagonists
MXPA01007366A (es) * 1999-01-21 2002-06-04 Metamorphix Inc Inhibidores de factores de crecimiento y diferenciacion y usos de los mismos.
AU4951501A (en) * 2000-03-27 2001-10-08 Genetics Inst Methods for purifying highly anionic proteins

Also Published As

Publication number Publication date
US20030050450A1 (en) 2003-03-13
EP1404428A4 (de) 2004-12-08
EP1404428B1 (de) 2006-07-19
EP1714982A2 (de) 2006-10-25
AU2008203302B2 (en) 2011-10-27
CA2448788A1 (en) 2002-12-12
CA2448788C (en) 2010-08-24
EP1714982B1 (de) 2009-01-21
DK1714982T3 (da) 2009-05-04
ATE333469T1 (de) 2006-08-15
US7223848B2 (en) 2007-05-29
DK1404428T3 (da) 2006-10-30
JP4288153B2 (ja) 2009-07-01
JP2004537042A (ja) 2004-12-09
EP1714982A3 (de) 2006-12-20
ES2266535T3 (es) 2007-03-01
WO2002098531A1 (en) 2002-12-12
EP1404428A1 (de) 2004-04-07
DE60231016D1 (de) 2009-03-12
AU2008203302A1 (en) 2008-08-14
ES2321539T3 (es) 2009-06-08
DE60213248T2 (de) 2006-11-30
NZ530025A (en) 2005-06-24
DE60213248D1 (de) 2006-08-31

Similar Documents

Publication Publication Date Title
ATE421536T1 (de) Verfahren zur reinigung stark anionischer proteine
DE60030323D1 (de) Verfahren und verbindungen zur verlängerung der halbwertzeiten bei der ausscheidung von biowirksamen verbindungen
ATE342353T1 (de) Abtrennung von plasmin(ogen) aus proteinlösungen
AU2658397A (en) Purification of tissue plasminogen activator (tpa)
ATE278785T1 (de) Neues verfahren zur gewinnung und reinigung von fusionsproteinen
ATE200293T1 (de) Verfahren zur chromatographischen reinigung und trennung von nucleinsäuregemischen
WO2002057290A8 (en) Novel fibronectin epitopes and proteinaceous molecules capable of binding said epitopes
ATE449104T1 (de) Verfahren zur reinigung von fibrinogen
DE60104833D1 (de) Verfahren zur abtrennung von einer basischen aminosäure aus fermentationsbrühen
DE50012831D1 (de) Verfahren zur verbindung von molekularen substanzen
DE69435020D1 (en) Protein-tyrosin-phosphatase ptp-s31
ATE417056T1 (de) Verfahren zur proteinreinigung
Vosbeck et al. The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). Specificity and immobilization of aminopeptidase.
DE60140493D1 (de) Verfahren zur Reinigung stark anionischer Proteine
ATE242782T1 (de) Verfahren zur herstellung von peptiden aus biologischen flüssigkeiten und nach diesem verfahren herstellbare peptide
ATE215964T1 (de) Phrothrombin und thrombin bindende peptide.
ATE278699T1 (de) Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens
DE60039533D1 (de) Verbessertes verfahren zur herstellung von 2s,3s-n-isobutyl-n-(2-hydroxy-3-amino-4-phenylbutyl)-p-nitrobenzenesulfonylamid hydrochlorid und anderen derivaten von 2-hydroxy-1,3-diamin
PL340728A1 (en) Histamine and serotonin bonding molecules
WO2004007536A3 (en) Interactions of the epstein-barr virus protein ebna1, and uses thereof
DE69621714D1 (de) Verfahren zur herstellung von trans-4-hydroxy-l-prolin
ATE220419T1 (de) Verfahren zur herstellung von cis-3-hydroxy-l- prolin
SE9303822D0 (sv) Method for purification
CA2193867A1 (en) Hydrophobic chromatographic resins with ionizable groups
ATE380197T1 (de) Verfahren zur reinigung von epi-hne proteinen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties