DK1714982T3 - Fremgangsmåde til rensning af stærkt anioniske proteiner - Google Patents

Fremgangsmåde til rensning af stærkt anioniske proteiner

Info

Publication number
DK1714982T3
DK1714982T3 DK06010184T DK06010184T DK1714982T3 DK 1714982 T3 DK1714982 T3 DK 1714982T3 DK 06010184 T DK06010184 T DK 06010184T DK 06010184 T DK06010184 T DK 06010184T DK 1714982 T3 DK1714982 T3 DK 1714982T3
Authority
DK
Denmark
Prior art keywords
purification
containing molecules
mixture
highly anionic
anionic proteins
Prior art date
Application number
DK06010184T
Other languages
English (en)
Inventor
Jonathan L Coffmann
William B Foster
Shujun Sun
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of DK1714982T3 publication Critical patent/DK1714982T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK06010184T 2001-06-05 2002-06-05 Fremgangsmåde til rensning af stærkt anioniske proteiner DK1714982T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05
PCT/US2002/018081 WO2002098531A1 (en) 2001-06-05 2002-06-05 Methods for purifying highly anionic proteins

Publications (1)

Publication Number Publication Date
DK1714982T3 true DK1714982T3 (da) 2009-05-04

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
DK02749564T DK1404428T3 (da) 2001-06-05 2002-06-05 Fremgangsmåder til oprensning af stærkt anioniske proteiner
DK06010184T DK1714982T3 (da) 2001-06-05 2002-06-05 Fremgangsmåde til rensning af stærkt anioniske proteiner

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK02749564T DK1404428T3 (da) 2001-06-05 2002-06-05 Fremgangsmåder til oprensning af stærkt anioniske proteiner

Country Status (11)

Country Link
US (1) US7223848B2 (da)
EP (2) EP1404428B1 (da)
JP (1) JP4288153B2 (da)
AT (2) ATE421536T1 (da)
AU (1) AU2008203302B2 (da)
CA (1) CA2448788C (da)
DE (2) DE60213248T2 (da)
DK (2) DK1404428T3 (da)
ES (2) ES2266535T3 (da)
NZ (1) NZ530025A (da)
WO (1) WO2002098531A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
WO2007075283A2 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
WO2007081906A2 (en) * 2006-01-06 2007-07-19 Amgen Inc. Purification by column-chromatography using eluants containing organic solvents
WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
TWI456062B (zh) 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2089429B1 (en) * 2006-10-19 2014-11-26 Janssen Biotech, Inc. Process for preparing unaggregated antibody fc domains
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
MX2011004201A (es) 2008-10-20 2011-05-24 Abbott Lab Desactivacion viral durante purificacion de anticuerpos.
CN102272145B (zh) * 2009-01-12 2014-07-30 通用电气健康护理生物科学股份公司 亲和色谱基质
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
EP2513134B1 (en) 2009-12-18 2017-09-06 Novartis AG Wash solution and method for affinity chromatography
BR112013030628B1 (pt) 2011-06-01 2022-01-25 Novartis Ag Método para produção de uma proteína purificada de interesse utilizando uma matriz de cromatografia de afinidade, e método para produção de um anticorpo purificado, fragmento de anticorpo ou proteína de fusão de fc utilizando uma coluna de proteína a
TWI787710B (zh) * 2020-01-20 2022-12-21 中國大陸商上海藥明生物技術有限公司 用於親和層析的新型清洗緩衝液

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US4983722A (en) 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) * 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US6277975B1 (en) * 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5843707A (en) * 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US5429746A (en) 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
DK0679718T3 (da) * 1994-04-09 2001-10-22 Hoffmann La Roche Fremgangsmåde til fremstilling af alfa-interferon
US6008328A (en) * 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) * 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) * 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) * 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
ES2293662T3 (es) * 1996-11-15 2008-03-16 Genentech, Inc. Purificacion de neurotrofinas.
EP0950067B1 (en) * 1996-11-27 2007-08-29 Genentech, Inc. Affinity purification of polypeptide on protein a matrix
EP1115742B1 (en) * 1998-09-24 2006-03-29 Pharming Intellectual Property BV Purification of fibrinogen from milk by use of cation exchange chromatography
MXPA01004310A (es) 1998-10-30 2003-06-06 Cbr Lab Inc Inhibicion de diferenciacion de celulas t citotoxicas mediante antagonistas de ligando de p-selectina (psgl).
AU772694B2 (en) 1999-01-21 2004-05-06 Metamorphix International, Inc. Growth differentiation factor inhibitors and uses therefor
JP5025874B2 (ja) * 2000-03-27 2012-09-12 ジェネティクス インスティテュート，エルエルシー 高アニオン性タンパク質の精製方法

Also Published As

Publication number Publication date
EP1714982B1 (en) 2009-01-21
EP1714982A3 (en) 2006-12-20
DE60231016D1 (de) 2009-03-12
EP1404428A4 (en) 2004-12-08
ES2266535T3 (es) 2007-03-01
CA2448788C (en) 2010-08-24
EP1404428A1 (en) 2004-04-07
ATE333469T1 (de) 2006-08-15
DE60213248D1 (de) 2006-08-31
JP4288153B2 (ja) 2009-07-01
DK1404428T3 (da) 2006-10-30
AU2008203302B2 (en) 2011-10-27
JP2004537042A (ja) 2004-12-09
ES2321539T3 (es) 2009-06-08
CA2448788A1 (en) 2002-12-12
AU2008203302A1 (en) 2008-08-14
DE60213248T2 (de) 2006-11-30
EP1714982A2 (en) 2006-10-25
NZ530025A (en) 2005-06-24
EP1404428B1 (en) 2006-07-19
US7223848B2 (en) 2007-05-29
ATE421536T1 (de) 2009-02-15
WO2002098531A1 (en) 2002-12-12
US20030050450A1 (en) 2003-03-13

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