ATE335825T1 - Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation - Google Patents

Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation

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Publication number
ATE335825T1
ATE335825T1 AT00969312T AT00969312T ATE335825T1 AT E335825 T1 ATE335825 T1 AT E335825T1 AT 00969312 T AT00969312 T AT 00969312T AT 00969312 T AT00969312 T AT 00969312T AT E335825 T1 ATE335825 T1 AT E335825T1
Authority
AT
Austria
Prior art keywords
thermostable
nucleic acid
enzyme
amplification
accuracy
Prior art date
Application number
AT00969312T
Other languages
English (en)
Inventor
Waltraud Ankenbauer
Frank Laue
Harald Sobek
Michael Greif
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE335825T1 publication Critical patent/ATE335825T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00969312T 1999-09-28 2000-09-27 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation ATE335825T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP99119268A EP1088891B1 (de) 1999-09-28 1999-09-28 Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation

Publications (1)

Publication Number Publication Date
ATE335825T1 true ATE335825T1 (de) 2006-09-15

Family

ID=8239082

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99119268T ATE286981T1 (de) 1999-09-28 1999-09-28 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
AT00969312T ATE335825T1 (de) 1999-09-28 2000-09-27 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT99119268T ATE286981T1 (de) 1999-09-28 1999-09-28 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation

Country Status (14)

Country Link
US (2) US7030220B1 (de)
EP (2) EP1088891B1 (de)
JP (1) JP3655240B2 (de)
CN (1) CN1185347C (de)
AT (2) ATE286981T1 (de)
AU (1) AU777229B2 (de)
CA (1) CA2351634C (de)
DE (2) DE69923180T2 (de)
ES (1) ES2269189T3 (de)
IL (1) IL143141A0 (de)
NO (1) NO20012561L (de)
NZ (1) NZ511759A (de)
RU (1) RU2260055C2 (de)
WO (1) WO2001023583A2 (de)

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EP1277841B1 (de) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
CA2409775C (en) * 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US9181534B1 (en) 2001-12-21 2015-11-10 Agilent Technologies Inc. High fidelity DNA polymerase compositions and uses thereof
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
EP1502958A1 (de) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
WO2006061994A1 (ja) * 2004-12-08 2006-06-15 Takeshi Yamamoto 遺伝子配列検査法
US7922177B2 (en) * 2005-05-18 2011-04-12 Diamond Game Enterprises, Inc. Ticket strips that encourage multiple ticket purchasing
US20090170090A1 (en) * 2005-11-18 2009-07-02 Bioline Limited Method for Enhancing Enzymatic DNA Polymerase Reactions
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8409805B2 (en) * 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
US8679757B2 (en) 2009-03-24 2014-03-25 Asuragen, Inc. PCR methods for characterizing the 5′ untranslated region of the FMR1 and FMR2 genes
US8700381B2 (en) * 2009-04-16 2014-04-15 Koninklijke Philips N.V. Methods for nucleic acid quantification
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CA2802239C (en) 2010-06-18 2016-08-23 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
EP2582806B1 (de) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
WO2011157432A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
JP5926248B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US8759062B2 (en) 2010-06-18 2014-06-24 Roche Molecular Systems, Inc. DNA polymerases with increased 3′- mismatch discrimination
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
US8765435B2 (en) 2011-02-15 2014-07-01 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
EP2559774A1 (de) * 2011-08-17 2013-02-20 Roche Diagniostics GmbH Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes
CN103998603B (zh) 2011-12-08 2016-05-25 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
ES2668448T3 (es) 2011-12-08 2018-05-18 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
ES2759023T3 (es) 2012-07-20 2020-05-07 Asuragen Inc Genotipificación completa de FMR1
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
US11739306B2 (en) 2018-09-13 2023-08-29 Roche Molecular Systems, Inc. Mutant DNA polymerase(s) with improved strand displacement ability
US20250320476A1 (en) * 2020-10-02 2025-10-16 National University Of Singapore A DNA Assembly Mix And Method Of Uses Thereof

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JP3761197B2 (ja) * 1995-12-27 2006-03-29 タカラバイオ株式会社 新規ｄｎａポリメラーゼ
AU7683998A (en) * 1997-04-08 1998-10-30 Rockfeller University, The Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
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DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
EP1175501A4 (de) 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
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EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung

Also Published As

Publication number Publication date
ES2269189T3 (es) 2007-04-01
NO20012561L (no) 2001-07-18
JP3655240B2 (ja) 2005-06-02
WO2001023583A2 (en) 2001-04-05
DE60029927D1 (de) 2006-09-21
RU2260055C2 (ru) 2005-09-10
IL143141A0 (en) 2002-04-21
DE69923180D1 (de) 2005-02-17
AU7907700A (en) 2001-04-30
DE69923180T2 (de) 2006-01-12
US7410782B2 (en) 2008-08-12
CA2351634A1 (en) 2001-04-05
CN1343254A (zh) 2002-04-03
EP1144653A2 (de) 2001-10-17
EP1088891B1 (de) 2005-01-12
US20060078928A1 (en) 2006-04-13
EP1144653B1 (de) 2006-08-09
DE60029927T2 (de) 2007-03-08
JP2003510084A (ja) 2003-03-18
CA2351634C (en) 2007-02-20
AU777229B2 (en) 2004-10-07
EP1088891A1 (de) 2001-04-04
NO20012561D0 (no) 2001-05-25
NZ511759A (en) 2003-12-19
US7030220B1 (en) 2006-04-18
WO2001023583A3 (en) 2001-10-25
ATE286981T1 (de) 2005-01-15
CN1185347C (zh) 2005-01-19

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