NO20012561L - Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro - Google Patents

Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro

Info

Publication number
NO20012561L
NO20012561L NO20012561A NO20012561A NO20012561L NO 20012561 L NO20012561 L NO 20012561L NO 20012561 A NO20012561 A NO 20012561A NO 20012561 A NO20012561 A NO 20012561A NO 20012561 L NO20012561 L NO 20012561L
Authority
NO
Norway
Prior art keywords
thermostable
nucleic acid
amplification
fidelity
vitro
Prior art date
Application number
NO20012561A
Other languages
English (en)
Norwegian (no)
Other versions
NO20012561D0 (no
Inventor
Waltraud Ankenbauer
Frank Laue
Harald Sobek
Michael Greif
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of NO20012561D0 publication Critical patent/NO20012561D0/no
Publication of NO20012561L publication Critical patent/NO20012561L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
NO20012561A 1999-09-28 2001-05-25 Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro NO20012561L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99119268A EP1088891B1 (de) 1999-09-28 1999-09-28 Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
PCT/EP2000/009423 WO2001023583A2 (en) 1999-09-28 2000-09-27 Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro

Publications (2)

Publication Number Publication Date
NO20012561D0 NO20012561D0 (no) 2001-05-25
NO20012561L true NO20012561L (no) 2001-07-18

Family

ID=8239082

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20012561A NO20012561L (no) 1999-09-28 2001-05-25 Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro

Country Status (14)

Country Link
US (2) US7030220B1 (de)
EP (2) EP1088891B1 (de)
JP (1) JP3655240B2 (de)
CN (1) CN1185347C (de)
AT (2) ATE286981T1 (de)
AU (1) AU777229B2 (de)
CA (1) CA2351634C (de)
DE (2) DE69923180T2 (de)
ES (1) ES2269189T3 (de)
IL (1) IL143141A0 (de)
NO (1) NO20012561L (de)
NZ (1) NZ511759A (de)
RU (1) RU2260055C2 (de)
WO (1) WO2001023583A2 (de)

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ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
EP1277841B1 (de) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US9181534B1 (en) 2001-12-21 2015-11-10 Agilent Technologies Inc. High fidelity DNA polymerase compositions and uses thereof
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
EP1502958A1 (de) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
KR20070090233A (ko) * 2004-12-08 2007-09-05 타케시 야마모토 유전자 서열 검사법
US7922177B2 (en) * 2005-05-18 2011-04-12 Diamond Game Enterprises, Inc. Ticket strips that encourage multiple ticket purchasing
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US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8409805B2 (en) * 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
ES2629400T3 (es) 2009-03-24 2017-08-09 Asuragen, Inc. Métodos de PCR para caracterizar la región 5' no traducida de los genes FMR1 y FMR2
BRPI1006682B1 (pt) * 2009-04-16 2020-04-22 Koninl Philips Electronics Nv método para a quantificação relativa de no mínimo um ácido nucleico alvo em uma amostra, método para a quantificação absoluta de no mínimo um ácido nucleico alvo em uma amostra, meios legíveis por máquina e equipamento para a análise das amostras de ácido nucleico
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP2582806B1 (de) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
EP2582803B1 (de) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
EP2675897B1 (de) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
EP2559774A1 (de) * 2011-08-17 2013-02-20 Roche Diagniostics GmbH Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes
EP2788480B1 (de) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
US9371560B2 (en) 2012-07-20 2016-06-21 Asuragen, Inc. Comprehensive FMR1 genotyping
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ． ホフマン－ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ
CN116391042A (zh) * 2020-10-02 2023-07-04 新加坡国立大学 Dna组装混合物和其使用方法
WO2026153862A1 (en) 2025-01-14 2026-07-23 F. Hoffmann-La Roche Ag Dna polymerases with improved activity

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WO1990012115A1 (en) * 1989-03-21 1990-10-18 Collaborative Research, Inc. A dna diagnostic test using an exonuclease activity
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
WO1994023066A1 (en) * 1993-03-30 1994-10-13 United States Biochemical Corporation Use of exonuclease in dna sequencing
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
CN1552869A (zh) * 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) * 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung

Also Published As

Publication number Publication date
DE60029927D1 (de) 2006-09-21
EP1088891A1 (de) 2001-04-04
DE69923180T2 (de) 2006-01-12
DE69923180D1 (de) 2005-02-17
ATE286981T1 (de) 2005-01-15
NO20012561D0 (no) 2001-05-25
EP1088891B1 (de) 2005-01-12
CA2351634A1 (en) 2001-04-05
DE60029927T2 (de) 2007-03-08
ES2269189T3 (es) 2007-04-01
NZ511759A (en) 2003-12-19
CN1185347C (zh) 2005-01-19
WO2001023583A3 (en) 2001-10-25
CA2351634C (en) 2007-02-20
JP3655240B2 (ja) 2005-06-02
US7030220B1 (en) 2006-04-18
CN1343254A (zh) 2002-04-03
AU7907700A (en) 2001-04-30
JP2003510084A (ja) 2003-03-18
AU777229B2 (en) 2004-10-07
US20060078928A1 (en) 2006-04-13
EP1144653B1 (de) 2006-08-09
WO2001023583A2 (en) 2001-04-05
RU2260055C2 (ru) 2005-09-10
US7410782B2 (en) 2008-08-12
EP1144653A2 (de) 2001-10-17
IL143141A0 (en) 2002-04-21
ATE335825T1 (de) 2006-09-15

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