ATE335827T1 - Verfahren zur verbindung von dna - Google Patents
Verfahren zur verbindung von dnaInfo
- Publication number
- ATE335827T1 ATE335827T1 AT01914857T AT01914857T ATE335827T1 AT E335827 T1 ATE335827 T1 AT E335827T1 AT 01914857 T AT01914857 T AT 01914857T AT 01914857 T AT01914857 T AT 01914857T AT E335827 T1 ATE335827 T1 AT E335827T1
- Authority
- AT
- Austria
- Prior art keywords
- linear
- vector
- joining dna
- specific
- alone
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18740100P | 2000-03-07 | 2000-03-07 | |
| CA002317865A CA2317865A1 (en) | 2000-03-07 | 2000-09-01 | Dna joining method |
| US26377101P | 2001-01-25 | 2001-01-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE335827T1 true ATE335827T1 (de) | 2006-09-15 |
Family
ID=22688823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01914857T ATE335827T1 (de) | 2000-03-07 | 2001-03-07 | Verfahren zur verbindung von dna |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1263973B1 (de) |
| JP (1) | JP4854160B2 (de) |
| AT (1) | ATE335827T1 (de) |
| AU (1) | AU2001242128A1 (de) |
| CA (1) | CA2317865A1 (de) |
| DE (1) | DE60122118T2 (de) |
| WO (1) | WO2001066775A2 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1915446T3 (en) | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| WO2007120624A2 (en) * | 2006-04-10 | 2007-10-25 | Codon Devices, Inc. | Concerted nucleic acid assembly reactions |
| EP2358875B1 (de) | 2008-11-19 | 2015-08-12 | Amyris, Inc. | Zusammensetzungen und verfahren zur konstruktion von polynukleotiden |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| JP6594955B2 (ja) * | 2014-08-27 | 2019-10-23 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シントン形成 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
| US5580759A (en) * | 1994-02-03 | 1996-12-03 | Board Of Regents, The University Of Texas System | Construction of recombinant DNA by exonuclease recession |
| IL120338A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Single step DNA fragments assembly |
-
2000
- 2000-09-01 CA CA002317865A patent/CA2317865A1/en not_active Abandoned
-
2001
- 2001-03-07 AT AT01914857T patent/ATE335827T1/de not_active IP Right Cessation
- 2001-03-07 JP JP2001565378A patent/JP4854160B2/ja not_active Expired - Lifetime
- 2001-03-07 AU AU2001242128A patent/AU2001242128A1/en not_active Abandoned
- 2001-03-07 WO PCT/CA2001/000283 patent/WO2001066775A2/en not_active Ceased
- 2001-03-07 DE DE60122118T patent/DE60122118T2/de not_active Expired - Lifetime
- 2001-03-07 EP EP01914857A patent/EP1263973B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP4854160B2 (ja) | 2012-01-18 |
| EP1263973B1 (de) | 2006-08-09 |
| DE60122118T2 (de) | 2007-03-08 |
| WO2001066775A2 (en) | 2001-09-13 |
| EP1263973A2 (de) | 2002-12-11 |
| AU2001242128A1 (en) | 2001-09-17 |
| CA2317865A1 (en) | 2001-09-07 |
| WO2001066775A3 (en) | 2002-03-28 |
| DE60122118D1 (de) | 2006-09-21 |
| JP2003533979A (ja) | 2003-11-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |