ATE352568T1 - Faltung von polypeptiden - Google Patents

Faltung von polypeptiden

Info

Publication number
ATE352568T1
ATE352568T1 AT04006513T AT04006513T ATE352568T1 AT E352568 T1 ATE352568 T1 AT E352568T1 AT 04006513 T AT04006513 T AT 04006513T AT 04006513 T AT04006513 T AT 04006513T AT E352568 T1 ATE352568 T1 AT E352568T1
Authority
AT
Austria
Prior art keywords
buffer
polypeptides
folding
refolding
alcoholic
Prior art date
Application number
AT04006513T
Other languages
English (en)
Inventor
Stuart Builder
Phillip Lester
Roger Hart
David Reifsnyder
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE352568T1 publication Critical patent/ATE352568T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT04006513T 1993-08-20 1994-08-15 Faltung von polypeptiden ATE352568T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/110,664 US5663304A (en) 1993-08-20 1993-08-20 Refolding of misfolded insulin-like growth factor-I

Publications (1)

Publication Number Publication Date
ATE352568T1 true ATE352568T1 (de) 2007-02-15

Family

ID=22334224

Family Applications (2)

Application Number Title Priority Date Filing Date
AT94927917T ATE264872T1 (de) 1993-08-20 1994-08-15 Mit rekombinantem insulin-ähnlichen wachstumsfaktor igf-i vergleichbare rückfaltung von polypeptiden
AT04006513T ATE352568T1 (de) 1993-08-20 1994-08-15 Faltung von polypeptiden

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT94927917T ATE264872T1 (de) 1993-08-20 1994-08-15 Mit rekombinantem insulin-ähnlichen wachstumsfaktor igf-i vergleichbare rückfaltung von polypeptiden

Country Status (10)

Country Link
US (3) US5663304A (de)
EP (2) EP1486511B1 (de)
JP (1) JP3648242B2 (de)
AT (2) ATE264872T1 (de)
CA (1) CA2168552C (de)
DE (2) DE69434919T2 (de)
DK (2) DK1486511T3 (de)
ES (2) ES2219649T3 (de)
PT (2) PT1486511E (de)
WO (1) WO1995006064A1 (de)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779927A1 (de) * 1994-09-08 1997-06-25 Chiron Corporation Methode zur gesteigerten herstellung von insulin-ähnlichem wachstumsfaktor
US5650496A (en) * 1995-04-14 1997-07-22 Cephalon, Inc. IGF-I purification process
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
AU6167596A (en) * 1995-06-07 1996-12-30 Chiron Corporation Methods for purifying authentic igf from yeast hosts
US7071313B1 (en) * 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6265542B1 (en) 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
US6417330B1 (en) 1998-06-01 2002-07-09 Celtrix Pharmaceuticals, Inc. Insulin-like growth factor binding protein variants
SE9802454D0 (sv) * 1998-07-08 1998-07-08 Pharmacia & Upjohn Ab Production of peptides
JP4709383B2 (ja) 1998-09-22 2011-06-22 ジェネンテック， インコーポレイテッド Ｕｃｐ４
US7399751B2 (en) * 1999-11-04 2008-07-15 Sertoli Technologies, Inc. Production of a biological factor and creation of an immunologically privileged environment using genetically altered Sertoli cells
US7342102B2 (en) 1998-11-30 2008-03-11 Genentech, Inc. Uncoupling protein 5 (UCP5)
ATE492640T1 (de) * 1998-11-30 2011-01-15 Genentech Inc Loslösendes protein 5 - ucp5
CA2358609A1 (en) * 1999-01-07 2000-07-13 Takeda Chemical Industries, Ltd. Process for producing activated protein
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
DE19930676B4 (de) * 1999-07-02 2006-01-19 Sanofi-Aventis Deutschland Gmbh Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
US6395538B1 (en) 1999-07-16 2002-05-28 Human Genome Sciences, Inc. Method and system for providing real-time, in situ biomanufacturing process monitoring and control in response to IR spectroscopy
US7118737B2 (en) 2000-09-08 2006-10-10 Amylin Pharmaceuticals, Inc. Polymer-modified synthetic proteins
KR20030057529A (ko) * 2000-09-08 2003-07-04 그리폰 테라퓨틱스, 인코포레이티드 합성 적혈구생성 자극 단백질
EP1679318A1 (de) * 2000-11-27 2006-07-12 Rmf Dictagene S.A. Verfahren zur Rückfaltung von chemisch hergestellten Polypeptiden
US20060252917A1 (en) * 2001-02-08 2006-11-09 University Of Utah Methods for refolding conformationally constrained peptides
CN1553811A (zh) * 2001-09-07 2004-12-08 ӡ�����Ƽ��ɷ����޹�˾ 人组织尿激酶类型纤溶酶原激活物制剂
EP1314739A1 (de) * 2001-11-22 2003-05-28 Bayer Ag Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen
AU2003215272B2 (en) 2002-02-14 2008-04-03 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
US8143210B2 (en) 2002-02-14 2012-03-27 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
US20030224476A1 (en) * 2002-03-01 2003-12-04 Szu-Yi Chou Method of producing transglutaminase reactive compound
US7776545B2 (en) 2002-11-20 2010-08-17 The Board Of Trustees Of The Leland Stanford Junior University Diagnostic method for Celiac Sprue
US7189811B2 (en) * 2002-09-06 2007-03-13 National Institute Of Immunology Process for solubilization of recombinant proteins expressed as inclusion body
US20050272117A1 (en) * 2003-06-23 2005-12-08 Astrazeneca Ab Method
CA2531022C (en) * 2003-07-03 2014-09-09 Jannette Dufour Compositions containing sertoli cells and myoid cells and use thereof in cellular transplants
US7579313B2 (en) * 2003-11-18 2009-08-25 The Board Of Trustees Of The Leland Stanford Junior University Transglutaminase inhibitors and methods of use thereof
EP2495308A1 (de) * 2005-12-08 2012-09-05 Amgen Inc. Verbesserte Herstellung von Glykoproteinen unter Verwendung von Mangan
BRPI0621124A2 (pt) 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
US20090081145A1 (en) * 2005-12-23 2009-03-26 Aplagen Gmbh Process for forming disulphide bridges
AR062069A1 (es) * 2006-07-14 2008-10-15 Genentech Inc Replegado de proteinas recombinantes
WO2008077621A1 (de) * 2006-12-23 2008-07-03 Aplagen Gmbh Verfahren zur ausbildung von disulfidbrücken
WO2008115428A2 (en) * 2007-03-16 2008-09-25 The Board Of Trustees Of The Leland Stanford Junior University A scaleable manufacturing process for cysteine endoprotease b, isoform 2
WO2008115411A1 (en) * 2007-03-16 2008-09-25 The Board Of Trustees Of The Leland Stanford Junior University Combination enzyme therapy for digestion of dietary gluten
KR102055873B1 (ko) 2007-07-09 2019-12-13 제넨테크, 인크. 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지
CN102438657A (zh) * 2009-03-26 2012-05-02 沃萨乌整形外科公司 鉴别定向药物递送组合物的潜在组分的方法
EP3366692A1 (de) 2009-06-22 2018-08-29 Amgen, Inc Proteinneufaltung mit einem chemisch gesteuerten redoxzustand
AU2010266093B2 (en) 2009-06-25 2013-06-27 Amgen Inc. Capture purification processes for proteins expressed in a non-mammalian system
US9052323B2 (en) * 2009-08-27 2015-06-09 The University Of Kansas Osmolyte mixture for protein stabilization
JP2013513622A (ja) * 2009-12-14 2013-04-22 ユニバーシティ オブ マサチューセッツ 白内障及び老眼を阻害する方法
US8906421B2 (en) * 2011-06-21 2014-12-09 Med-Genesis, Llc Methods of dissolving blood clots and the like
PE20151747A1 (es) 2013-03-14 2015-12-18 Univ Massachusetts Metodo para inhibir cataratas y presbicia
MX376641B (es) 2015-11-13 2025-03-07 Univ Massachusetts Moléculas bifuncionales que contienen peg para uso en la inhibición de cataratas y presbicia.
US20220396787A1 (en) * 2021-06-11 2022-12-15 Acutis Diagnostics, Inc. Method for enhanced direct detection of microbial antigens from biological fluids
CN117603332B (zh) * 2023-11-23 2024-09-10 基因科技（上海）股份有限公司 重组人ny-eso-1蛋白的制备方法及其应用

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518256A (en) * 1977-05-23 1985-05-21 Sanders Associates, Inc. Enhanced accuracy optical radar
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
SE8303626D0 (sv) * 1983-06-23 1983-06-23 Kabigen Ab A recombinant plasmid a transformant microorganism, a polydoxyrebonucleotide segment, a process for producing a biologically active protein, and the protein thus produced
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
US5028531A (en) * 1984-03-19 1991-07-02 Fujisawa Pharmaceutical Company, Ltd. IGF-I fusion proteins; protection of IGF-I from degradation by host cell; and processes for the production thereof
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
US4572798A (en) * 1984-12-06 1986-02-25 Cetus Corporation Method for promoting disulfide bond formation in recombinant proteins
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
CA1260858A (en) * 1985-03-28 1989-09-26 Lawrence S. Cousens Expression using fused genes providing for protein product
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
ES2029785T3 (es) * 1985-10-21 1992-10-01 Fujisawa Pharmaceutical Co., Ltd. Procedimiento para producir factor de crecimiento tipo insulina i y plasmido para su produccion.
JPH0759598B2 (ja) * 1986-02-14 1995-06-28 藤沢薬品工業株式会社 ヒトインスリン様成長因子▲ｉ▼の製造法
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
EP0264074B1 (de) * 1986-10-09 1992-12-30 Fujisawa Pharmaceutical Co., Ltd. Expressionsvektor für menschlichen insulinähnlichen Wachstumsfaktor-I
JP2502563B2 (ja) * 1987-02-20 1996-05-29 松下電器産業株式会社 フロントパネルの外観仕上げ方法
US5104796A (en) * 1987-04-06 1992-04-14 International Minerals & Chemical Corp. High titer production of human somatomedin c
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
EP0288451B1 (de) * 1987-04-23 1994-08-10 Monsanto Company Sekretion eines dem Insulin ähnlichen Wachstumsfaktors in E. coli
ATE156491T1 (de) * 1987-05-11 1997-08-15 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
JPS63294796A (ja) * 1987-05-27 1988-12-01 Sagami Chem Res Center タンパク質の再賦活化方法
IL86455A0 (en) * 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
US4923967A (en) * 1988-09-26 1990-05-08 Eli Lilly And Company Purification and refolding of recombinant proteins
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
GB8915130D0 (en) * 1989-06-30 1989-08-23 Univ Dundee Enzyme production
EP0647714B1 (de) * 1989-07-19 1997-12-29 Tonen Corporation Peptidyl prolyl-cis.trans-isomerase
US5124444A (en) * 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5158875A (en) * 1989-08-25 1992-10-27 Amgen Inc. Production of biologically active insulin-like growth factor i from high expression host cell systems
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
JPH06500084A (ja) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ 生物学的に活性な化合物、その製造方法及びその利用
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
WO1993019084A1 (en) * 1992-03-24 1993-09-30 Synergen, Inc. Refolding and purification of insulin-like growth factor i

Also Published As

Publication number Publication date
ES2219649T3 (es) 2004-12-01
PT714406E (pt) 2004-09-30
JPH09501693A (ja) 1997-02-18
EP1486511B1 (de) 2007-01-24
US5756672A (en) 1998-05-26
JP3648242B2 (ja) 2005-05-18
ATE264872T1 (de) 2004-05-15
US5663304A (en) 1997-09-02
DE69433724D1 (de) 2004-05-27
US5808006A (en) 1998-09-15
CA2168552C (en) 2000-02-15
EP0714406A1 (de) 1996-06-05
WO1995006064A1 (en) 1995-03-02
EP0714406B1 (de) 2004-04-21
CA2168552A1 (en) 1995-03-02
DK0714406T3 (da) 2004-07-26
ES2280857T3 (es) 2007-09-16
DK1486511T3 (da) 2007-05-29
DE69434919D1 (de) 2007-03-15
DE69434919T2 (de) 2007-11-29
EP1486511A1 (de) 2004-12-15
DE69433724T2 (de) 2005-04-28
PT1486511E (pt) 2007-04-30

Similar Documents

Publication Publication Date Title
ATE352568T1 (de) Faltung von polypeptiden
EP0886648B8 (de) An den erythropoietin-rezeptor bindende zusammensetzungen und peptide
US4530787B1 (de)
KR950005324A (ko) 인슐린 제제
CA2168429A1 (en) Expression of fusion polypeptides transported out of cytoplasm without leader sequences
EP1686172A3 (de) Herztransplantate und dafür nützliche Zellzusammensetzungen
WO2000023472A3 (en) Interferon-beta fusion proteins and uses
AU3167289A (en) Low toxicity alloy composition for joining and sealing
CA2116066A1 (en) Stabilized composition of troponin for immunoassays and process for stabilizing troponin for immunoassays
CA2303165A1 (en) Buffers for stabilizing antigens
CA2157146A1 (en) Heterocyclyl-benzoylguanidines
NO971173L (no) Konserveringsmiddel
KR910016767A (ko) 신규한 펩티드
EP0373325A3 (de) Verfahren zum Lösen und Renaturieren von Somatotropin
van der Rohe et al. Barcelona Pavilion
CA2176655A1 (en) Stabilized composition of troponin for immunoassays and method of preparation of such a stabilized composition
ATE228847T1 (de) Inhibitoren von katalytischen antikörpern
AU564269B2 (en) Compositions containing s-adenosyl-l methionine
ATE294189T1 (de) Kristallisation von proteinen
KR890008166A (ko) 융합 단백질의 선택적 분해 방법
EP1739090A3 (de) Interferon Beta Fusions Proteine und deren Gebrauch
AU7294294A (en) 12-hetero substituted 6,11-ethano-6,11-dihydrobenzo(b) quinolizinium salts and compositions and method of use thereof
AU6243094A (en) Stable aqueous acid compositions thickened with polyacrylamide
EP0063328A3 (de) Sekretin enthaltende Zusammensetzungen und Verfahren zur Verhütung der Adsorption von Sekretin
JPH10158282A5 (de)

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1486511

Country of ref document: EP