ATE373723T1 - Verbessertes verfahren zum nachweis von proteolytischen enzymen - Google Patents

Verbessertes verfahren zum nachweis von proteolytischen enzymen

Info

Publication number
ATE373723T1
ATE373723T1 AT04706386T AT04706386T ATE373723T1 AT E373723 T1 ATE373723 T1 AT E373723T1 AT 04706386 T AT04706386 T AT 04706386T AT 04706386 T AT04706386 T AT 04706386T AT E373723 T1 ATE373723 T1 AT E373723T1
Authority
AT
Austria
Prior art keywords
improved method
proteolytic enzymes
detecting proteolytic
modified pro
detecting
Prior art date
Application number
AT04706386T
Other languages
English (en)
Inventor
Johan Verheijen
Jan Hanemaaijer
Original Assignee
Tno
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tno filed Critical Tno
Application granted granted Critical
Publication of ATE373723T1 publication Critical patent/ATE373723T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472Cysteine endopeptidases (3.4.22)
    • C12N9/6475Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT04706386T 2003-01-31 2004-01-29 Verbessertes verfahren zum nachweis von proteolytischen enzymen ATE373723T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03075319A EP1443116A1 (de) 2003-01-31 2003-01-31 Verbessertes Verfahren zum Nachweis von proteolytischen Enzymen

Publications (1)

Publication Number Publication Date
ATE373723T1 true ATE373723T1 (de) 2007-10-15

Family

ID=32605369

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04706386T ATE373723T1 (de) 2003-01-31 2004-01-29 Verbessertes verfahren zum nachweis von proteolytischen enzymen

Country Status (6)

Country Link
US (2) US7361485B2 (de)
EP (2) EP1443116A1 (de)
JP (1) JP4708326B2 (de)
AT (1) ATE373723T1 (de)
DE (1) DE602004009036T2 (de)
WO (1) WO2004067768A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005015756D1 (de) * 2004-12-23 2009-09-10 Tno Verfahren zum funktionellen nachweis von masps und dessen komplexen
WO2007091881A2 (en) * 2005-04-29 2007-08-16 Leiden University Medical Center Methods and means for detection of mycobacterium leprae infections
AU2012228407B2 (en) * 2011-03-11 2016-10-13 Merz Pharma Gmbh & Co. Kgaa Method for the determination of botulinum neurotoxin biological activity
US9402876B2 (en) 2013-11-27 2016-08-02 Industrial Technology Research Institute Method and pharmaceutical composition for hair growth
AU2022339955A1 (en) * 2021-09-02 2024-03-07 University Of Washington Multiplex, temporally resolved molecular signal recorder and related methods

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69528614T2 (de) * 1994-07-07 2003-06-18 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno, Delft Modifizierte Proenzyme als Substrate für proteolische Enzyme
EP0691409B1 (de) * 1994-07-07 2002-10-23 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Modifizierte Proenzyme als Substrate für proteolische Enzyme
US6270980B1 (en) * 1997-06-05 2001-08-07 Idun Pharmaceuticals, Inc. Rapid methods for identifying modifiers of cellular apoptosis activity
US6379950B1 (en) * 1998-01-09 2002-04-30 Thomas Jefferson University Recombinant, active caspases and uses thereof

Also Published As

Publication number Publication date
US20060240406A1 (en) 2006-10-26
US20080187945A1 (en) 2008-08-07
US7361485B2 (en) 2008-04-22
WO2004067768A3 (en) 2004-12-02
JP2006516900A (ja) 2006-07-13
WO2004067768A2 (en) 2004-08-12
JP4708326B2 (ja) 2011-06-22
DE602004009036T2 (de) 2008-06-19
DE602004009036D1 (de) 2007-10-31
EP1587952B1 (de) 2007-09-19
EP1443116A1 (de) 2004-08-04
EP1587952A2 (de) 2005-10-26

Similar Documents

Publication Publication Date Title
ATE547412T1 (de) Inhibitoren von serinproteasen, insbesondere hcv- ns3-ns4a-protease
ATE510214T1 (de) Krankheitsdiagnose
DE69528614D1 (de) Modifizierte Proenzyme als Substrate für proteolische Enzyme
ATE520986T1 (de) Proteasenachweis
DE69424663D1 (de) Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen
ATE475887T1 (de) Verfahren und zusammensetzungen zur isolierung von metastatischen krebszellen und anwendung zur messung des metastatischen potentials eines krebses
ATE431427T1 (de) Verbessertes verfahren zum nachweis von atp
DE60233142D1 (de) Verfahren und reagentien zum nachweis von endotoxin
IS5028A (is) Serínpróteasatálmar, einkum við lifrarbólgu C veiru NS3 próteasa
ATE377654T1 (de) Ubiquitin-ligase-assay
ATE478559T1 (de) Nachweis von protease und protease-aktivität mithilfe einer einzel-nanosteigerungs-sers-sonde
ATE496123T1 (de) Nukleinsäuren, welche für tramsmembran serin proteasen kodieren, die kodierten proteine und darauf basierende methoden
DE69938600D1 (de) Fusionsproteine und deren verwendung zur messung von protease-aktivität
DE602004026888D1 (de) Kinase- und phosphatasetests
DE602005013998D1 (de) Verfahren und reagenzien zur erkennung von melanomen
DK1762626T3 (da) Signalamplifikation under anvendelse af et syntetisk zymogen
ATE373723T1 (de) Verbessertes verfahren zum nachweis von proteolytischen enzymen
ATE352038T1 (de) Kit und verfahren zum nachweis von fäkalen parasiten
ATE371867T1 (de) Überwachung von zellen
ATE347105T1 (de) Verfahren, assay und kit zur quantifizierung von hiv-proteasehemmer
ATE307142T1 (de) Zweifarbiges fluorimetrisches proteaseassay
FI20040805A7 (fi) Menetelmä polyamidin modifioimiseksi
ATE288081T1 (de) Verfahren zum selektieren von inhibitoren für enzyme
DE60331414D1 (de) Aggrecanase-1- und -2-peptidsubstrate und verfahren
ATE321877T1 (de) Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1587952

Country of ref document: EP