ATE374782T1 - Verbessertes verfahren zur bisulfit-behandlung - Google Patents

Verbessertes verfahren zur bisulfit-behandlung

Info

Publication number
ATE374782T1
ATE374782T1 AT03019321T AT03019321T ATE374782T1 AT E374782 T1 ATE374782 T1 AT E374782T1 AT 03019321 T AT03019321 T AT 03019321T AT 03019321 T AT03019321 T AT 03019321T AT E374782 T1 ATE374782 T1 AT E374782T1
Authority
AT
Austria
Prior art keywords
solid phase
glass
nucleic acid
bisulfite
improved method
Prior art date
Application number
AT03019321T
Other languages
English (en)
Inventor
Christine Markert-Hahn
Dirk Block
Original Assignee
Hoffmann La Roche
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32071078&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE374782(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP02019097A external-priority patent/EP1394172A1/de
Application filed by Hoffmann La Roche, Epigenomics Ag filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE374782T1 publication Critical patent/ATE374782T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Iron (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT03019321T 2002-08-29 2003-08-27 Verbessertes verfahren zur bisulfit-behandlung ATE374782T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02019097A EP1394172A1 (de) 2002-08-29 2002-08-29 Verbessertes Verfahren Zur Bisulfit-Behandlung
EP02028114 2002-12-18

Publications (1)

Publication Number Publication Date
ATE374782T1 true ATE374782T1 (de) 2007-10-15

Family

ID=32071078

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03019321T ATE374782T1 (de) 2002-08-29 2003-08-27 Verbessertes verfahren zur bisulfit-behandlung

Country Status (14)

Country Link
US (2) US9394332B2 (de)
EP (2) EP1394173B9 (de)
JP (2) JP5153048B2 (de)
CN (1) CN1271081C (de)
AT (1) ATE374782T1 (de)
AU (1) AU2003236461B2 (de)
CA (1) CA2438327C (de)
CY (1) CY1107118T1 (de)
DE (1) DE60316642T2 (de)
DK (2) DK1394173T3 (de)
ES (2) ES2545577T3 (de)
HU (1) HUE027240T2 (de)
PT (1) PT1394173E (de)
SI (2) SI1829886T1 (de)

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AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
US7368239B2 (en) * 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
DE602004021192D1 (de) 2003-10-09 2009-07-02 Epigenomics Ag Verbesserte bisulfitkonversion von dna
WO2005100240A1 (ja) * 2004-04-08 2005-10-27 Toyo Boseki Kabushiki Kaisha Dnaの脱アミノ化のための組成物及びメチル化dnaの検出方法
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
WO2006034264A2 (en) * 2004-09-21 2006-03-30 Applera Corporation Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated dna analysis
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
WO2006103111A2 (en) 2005-04-01 2006-10-05 Epigenomics Ag Improved bisulfite conversion of dna
CN101331148B (zh) * 2005-12-14 2012-06-27 霍夫曼-拉罗奇有限公司 用于亚硫酸氢盐处理的新方法
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
EP2240603A4 (de) * 2008-01-03 2011-07-20 Univ Johns Hopkins Zusammensetzungen und verfahren für polynukleotidextraktion und methylierungsnachweis
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
HK1198547A1 (en) 2011-07-29 2015-05-15 剑桥表现遗传学有限公司 Methods for detection of nucleotide modification
ES2786984T3 (es) 2012-11-30 2020-10-14 Cambridge Epigenetix Ltd Agente oxidante para nucleótidos modificados
CA2981940A1 (en) * 2015-06-10 2016-12-15 Biocartis N.V. Improved detection of methylated dna
CN107922941A (zh) * 2015-06-15 2018-04-17 塞弗德公司 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量
CA3046636A1 (en) 2016-12-12 2018-06-21 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
EP3765637A1 (de) 2018-03-13 2021-01-20 Grail, Inc. Detektion und klassifizierung von anomalen fragmenten
CN108265050A (zh) * 2018-03-13 2018-07-10 普迈德（北京）科技有限公司 一种血浆直接亚硫酸氢盐转化的方法及其应用
AU2019249422B2 (en) 2018-04-02 2025-09-25 Grail, Inc. Methylation markers and targeted methylation probe panels
IL281741B2 (en) 2018-09-27 2026-04-01 Grail Inc Methylation markers and a targeted methylation detection panel
WO2020132572A1 (en) 2018-12-21 2020-06-25 Grail, Inc. Source of origin deconvolution based on methylation fragments in cell-free-dna samples
DE102019118332B4 (de) 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
EP4096694A4 (de) 2020-01-30 2024-01-24 Prognomiq Inc Lungenbiomarker und verfahren zur verwendung davon
JP7853277B2 (ja) * 2020-07-29 2026-04-28 エグザクト サイエンシーズ コーポレーション スルホン化ｄｎａの精製
US20220328134A1 (en) 2021-03-31 2022-10-13 PrognomIQ, Inc. Multi-omic assessment
US12387508B2 (en) 2021-09-10 2025-08-12 PrognomIQ, Inc. Direct classification of raw biomolecule measurement data
AU2022341187A1 (en) 2021-09-13 2024-03-21 PrognomIQ, Inc. Enhanced detection and quantitation of biomolecules
CN114317761A (zh) * 2022-02-23 2022-04-12 厦门飞朔生物技术有限公司 一种宫颈癌相关基因甲基化检测方法

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Also Published As

Publication number Publication date
AU2003236461A1 (en) 2004-03-18
EP1394173B1 (de) 2007-10-03
JP5153048B2 (ja) 2013-02-27
ES2294231T3 (es) 2008-04-01
DE60316642D1 (de) 2007-11-15
EP1394173A1 (de) 2004-03-03
PT1394173E (pt) 2007-11-28
HK1062688A1 (en) 2004-11-19
US9394332B2 (en) 2016-07-19
DK1394173T3 (da) 2008-02-04
EP1829886B1 (de) 2015-07-29
US20040241704A1 (en) 2004-12-02
SI1829886T1 (sl) 2015-12-31
EP1829886A3 (de) 2008-10-15
CA2438327C (en) 2008-04-15
CA2438327A1 (en) 2004-02-29
ES2545577T3 (es) 2015-09-14
EP1829886A2 (de) 2007-09-05
DE60316642T2 (de) 2008-01-31
JP2004089195A (ja) 2004-03-25
EP1394173B9 (de) 2008-10-15
AU2003236461B2 (en) 2009-05-28
JP2009106304A (ja) 2009-05-21
CN1271081C (zh) 2006-08-23
HUE027240T2 (en) 2016-10-28
CN1495192A (zh) 2004-05-12
US20160068888A1 (en) 2016-03-10
JP5310059B2 (ja) 2013-10-09
US9868756B2 (en) 2018-01-16
DK1829886T3 (en) 2015-08-17
SI1394173T1 (sl) 2008-02-29
CY1107118T1 (el) 2012-10-24

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