ATE374782T1 - Verbessertes verfahren zur bisulfit-behandlung - Google Patents
Verbessertes verfahren zur bisulfit-behandlungInfo
- Publication number
- ATE374782T1 ATE374782T1 AT03019321T AT03019321T ATE374782T1 AT E374782 T1 ATE374782 T1 AT E374782T1 AT 03019321 T AT03019321 T AT 03019321T AT 03019321 T AT03019321 T AT 03019321T AT E374782 T1 ATE374782 T1 AT E374782T1
- Authority
- AT
- Austria
- Prior art keywords
- solid phase
- glass
- nucleic acid
- bisulfite
- improved method
- Prior art date
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 239000011521 glass Substances 0.000 abstract 4
- 239000007790 solid phase Substances 0.000 abstract 4
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 230000009615 deamination Effects 0.000 abstract 2
- 238000006481 deamination reaction Methods 0.000 abstract 2
- 230000006326 desulfonation Effects 0.000 abstract 2
- 238000005869 desulfonation reaction Methods 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Compounds Of Iron (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02019097A EP1394172A1 (de) | 2002-08-29 | 2002-08-29 | Verbessertes Verfahren Zur Bisulfit-Behandlung |
| EP02028114 | 2002-12-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE374782T1 true ATE374782T1 (de) | 2007-10-15 |
Family
ID=32071078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03019321T ATE374782T1 (de) | 2002-08-29 | 2003-08-27 | Verbessertes verfahren zur bisulfit-behandlung |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US9394332B2 (de) |
| EP (2) | EP1829886B1 (de) |
| JP (2) | JP5153048B2 (de) |
| CN (1) | CN1271081C (de) |
| AT (1) | ATE374782T1 (de) |
| AU (1) | AU2003236461B2 (de) |
| CA (1) | CA2438327C (de) |
| CY (1) | CY1107118T1 (de) |
| DE (1) | DE60316642T2 (de) |
| DK (2) | DK1394173T3 (de) |
| ES (2) | ES2545577T3 (de) |
| HU (1) | HUE027240T2 (de) |
| PT (1) | PT1394173E (de) |
| SI (2) | SI1394173T1 (de) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10154317B4 (de) * | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| AU2003236461B2 (en) * | 2002-08-29 | 2009-05-28 | Epigenomics Ag | Improved method for bisulfite treatment |
| US7534873B2 (en) * | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| US7371526B2 (en) * | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| US7262013B2 (en) * | 2003-08-29 | 2007-08-28 | Applera Corporation | Bisulfite method |
| US7368239B2 (en) * | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| DE602004021192D1 (de) | 2003-10-09 | 2009-07-02 | Epigenomics Ag | Verbesserte bisulfitkonversion von dna |
| WO2005100240A1 (ja) * | 2004-04-08 | 2005-10-27 | Toyo Boseki Kabushiki Kaisha | Dnaの脱アミノ化のための組成物及びメチル化dnaの検出方法 |
| EP1632578A1 (de) * | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | Methode zur Dekontamination der DNA |
| CA2581140A1 (en) * | 2004-09-21 | 2006-03-30 | Applera Corporation | Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated dna analysis |
| US7658288B2 (en) | 2004-11-08 | 2010-02-09 | Applied Biosystems, Llc | Bisulfite conversion reagent |
| WO2006103111A2 (en) | 2005-04-01 | 2006-10-05 | Epigenomics Ag | Improved bisulfite conversion of dna |
| EP1963352B1 (de) * | 2005-12-14 | 2010-09-08 | Roche Diagnostics GmbH | Neues verfahren für die bisulfitbehandlung |
| US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
| US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| WO2009088987A2 (en) * | 2008-01-03 | 2009-07-16 | The Johns Hopkins University | Compositions and methods for polynucleotide extraction and methylation detection |
| US9115386B2 (en) | 2008-09-26 | 2015-08-25 | Children's Medical Center Corporation | Selective oxidation of 5-methylcytosine by TET-family proteins |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| EP3141616B1 (de) | 2011-07-29 | 2020-05-13 | Cambridge Epigenetix Limited | Methoden zum nachweis der nukleotidmodifikation |
| US10563248B2 (en) | 2012-11-30 | 2020-02-18 | Cambridge Epigenetix Limited | Oxidizing agent for modified nucleotides |
| JP6681414B2 (ja) * | 2015-06-10 | 2020-04-15 | バイオカーティス エヌ ヴイ | メチル化ｄｎａの改善された検出 |
| CN107922941A (zh) | 2015-06-15 | 2018-04-17 | 塞弗德公司 | 在自动化反应盒中DNA甲基化的整合的纯化和测量以及突变和/或mRNA表达水平的共同测量 |
| EP3551332B1 (de) | 2016-12-12 | 2024-01-10 | Cepheid | Integrierte reinigung und messung der dna-methylierung und ko-messung von mutationen und/oder mrna-expressionsniveaus in einer automatisierten reaktionskartusche |
| AU2019234843B2 (en) | 2018-03-13 | 2025-12-04 | Grail, Inc. | Anomalous fragment detection and classification |
| CN108265050A (zh) * | 2018-03-13 | 2018-07-10 | 普迈德（北京）科技有限公司 | 一种血浆直接亚硫酸氢盐转化的方法及其应用 |
| WO2019195268A2 (en) | 2018-04-02 | 2019-10-10 | Grail, Inc. | Methylation markers and targeted methylation probe panels |
| CA3111887A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| EP3899953A1 (de) | 2018-12-21 | 2021-10-27 | Grail, Inc. | Ursprungsquellendekonvolution auf basis von methylierungsfragmenten in zellfreien dna-proben |
| DE102019118332B4 (de) | 2019-07-07 | 2022-04-07 | Ist Innuscreen Gmbh | Verfahren und testkit zur bisulfitmodifizierung von dna |
| EP4096694A4 (de) | 2020-01-30 | 2024-01-24 | Prognomiq Inc | Lungenbiomarker und verfahren zur verwendung davon |
| CN116157519A (zh) * | 2020-07-29 | 2023-05-23 | 精密科学公司 | 经磺化dna的纯化 |
| US20220328134A1 (en) | 2021-03-31 | 2022-10-13 | PrognomIQ, Inc. | Multi-omic assessment |
| US12387508B2 (en) | 2021-09-10 | 2025-08-12 | PrognomIQ, Inc. | Direct classification of raw biomolecule measurement data |
| EP4711762A1 (de) | 2021-09-13 | 2026-03-18 | Prognomiq Inc | Verbesserter nachweis und quantifizierung von biomolekülen |
| CN114317761A (zh) * | 2022-02-23 | 2022-04-12 | 厦门飞朔生物技术有限公司 | 一种宫颈癌相关基因甲基化检测方法 |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| DE3724442A1 (de) | 1987-07-23 | 1989-02-02 | Europ Lab Molekularbiolog | Verfahren und vorrichtung zur reinigung von m-13-phagen-dna |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| WO1990001069A1 (en) | 1988-07-20 | 1990-02-08 | Segev Diagnostics, Inc. | Process for amplifying and detecting nucleic acid sequences |
| AU640626B2 (en) | 1988-11-21 | 1993-09-02 | Dynal As | Nucleic acid probes |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| AU669398B2 (en) | 1992-11-13 | 1996-06-06 | Becton Dickinson & Company | Boron silicates aluminum silicates phosphosilicates and purification of DNA |
| US5438127A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| US5503816A (en) | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
| US5552277A (en) | 1994-07-19 | 1996-09-03 | The Johns Hopkins University School Of Medicine | Genetic diagnosis of prostate cancer |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| EP0912766B2 (de) | 1996-06-04 | 2011-12-14 | University of Utah Research Foundation | Überwachung der hybridisierung während pcr |
| CA2286573C (en) * | 1997-04-16 | 2004-10-26 | Immunological Associates Of Denver | Nucleic acid archiving |
| GB9714716D0 (en) * | 1997-07-11 | 1997-09-17 | Brax Genomics Ltd | Characterising nucleic acids |
| DE19743518A1 (de) | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| DE59912604D1 (de) | 1998-02-04 | 2005-11-03 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| JP3561235B2 (ja) | 1998-11-30 | 2004-09-02 | ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸を精製するための磁性粒子 |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| US6423536B1 (en) | 1999-08-02 | 2002-07-23 | Molecular Dynamics, Inc. | Low volume chemical and biochemical reaction system |
| DK1232502T3 (da) * | 1999-11-17 | 2006-05-22 | Roche Diagnostics Gmbh | Magnetiske glaspartikler, fremgangsmåde til deres fremstilling samt anvendelser deraf |
| DE19958042A1 (de) | 1999-12-03 | 2001-06-21 | Invitek Gmbh | Oberflächenmodifizierte Trägermaterialien zur Bindung biologischer Materialien, Verfahren zu ihrer Herstellung und ihre Verwendung |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| US20030152950A1 (en) | 2001-06-27 | 2003-08-14 | Garner Harold R. | Identification of chemically modified polymers |
| WO2003012124A2 (en) * | 2001-07-31 | 2003-02-13 | New York Institute Of Technology | Methods for immobilizing molecules to a solid phase and uses thereof |
| DE10154317B4 (de) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| AU2003236461B2 (en) | 2002-08-29 | 2009-05-28 | Epigenomics Ag | Improved method for bisulfite treatment |
| EP1394172A1 (de) | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Verbessertes Verfahren Zur Bisulfit-Behandlung |
| US7501240B2 (en) * | 2003-12-02 | 2009-03-10 | Roche Molecular Systems, Inc. | Method for bisulfite treatment |
| EP1963352B1 (de) * | 2005-12-14 | 2010-09-08 | Roche Diagnostics GmbH | Neues verfahren für die bisulfitbehandlung |
-
2003
- 2003-08-25 AU AU2003236461A patent/AU2003236461B2/en not_active Ceased
- 2003-08-25 US US10/647,720 patent/US9394332B2/en active Active
- 2003-08-27 ES ES07012188.4T patent/ES2545577T3/es not_active Expired - Lifetime
- 2003-08-27 EP EP07012188.4A patent/EP1829886B1/de not_active Revoked
- 2003-08-27 SI SI200331015T patent/SI1394173T1/sl unknown
- 2003-08-27 EP EP03019321A patent/EP1394173B9/de not_active Expired - Lifetime
- 2003-08-27 PT PT03019321T patent/PT1394173E/pt unknown
- 2003-08-27 SI SI200332443T patent/SI1829886T1/sl unknown
- 2003-08-27 DK DK03019321T patent/DK1394173T3/da active
- 2003-08-27 DK DK07012188.4T patent/DK1829886T3/en active
- 2003-08-27 DE DE60316642T patent/DE60316642T2/de not_active Expired - Lifetime
- 2003-08-27 ES ES03019321T patent/ES2294231T3/es not_active Expired - Lifetime
- 2003-08-27 HU HUE07012188A patent/HUE027240T2/en unknown
- 2003-08-27 AT AT03019321T patent/ATE374782T1/de active
- 2003-08-28 CN CNB031602835A patent/CN1271081C/zh not_active Expired - Fee Related
- 2003-08-28 CA CA002438327A patent/CA2438327C/en not_active Expired - Fee Related
- 2003-08-29 JP JP2003306141A patent/JP5153048B2/ja not_active Expired - Lifetime
-
2008
- 2008-01-02 CY CY20081100002T patent/CY1107118T1/el unknown
-
2009
- 2009-02-13 JP JP2009031400A patent/JP5310059B2/ja not_active Expired - Lifetime
-
2015
- 2015-08-24 US US14/833,738 patent/US9868756B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1829886A3 (de) | 2008-10-15 |
| DK1829886T3 (en) | 2015-08-17 |
| CN1271081C (zh) | 2006-08-23 |
| US9868756B2 (en) | 2018-01-16 |
| DK1394173T3 (da) | 2008-02-04 |
| EP1394173B9 (de) | 2008-10-15 |
| ES2545577T3 (es) | 2015-09-14 |
| SI1394173T1 (sl) | 2008-02-29 |
| US20160068888A1 (en) | 2016-03-10 |
| EP1394173A1 (de) | 2004-03-03 |
| DE60316642T2 (de) | 2008-01-31 |
| AU2003236461B2 (en) | 2009-05-28 |
| US9394332B2 (en) | 2016-07-19 |
| JP2004089195A (ja) | 2004-03-25 |
| CA2438327A1 (en) | 2004-02-29 |
| CY1107118T1 (el) | 2012-10-24 |
| EP1829886A2 (de) | 2007-09-05 |
| EP1394173B1 (de) | 2007-10-03 |
| JP2009106304A (ja) | 2009-05-21 |
| ES2294231T3 (es) | 2008-04-01 |
| CA2438327C (en) | 2008-04-15 |
| HUE027240T2 (en) | 2016-10-28 |
| DE60316642D1 (de) | 2007-11-15 |
| US20040241704A1 (en) | 2004-12-02 |
| HK1062688A1 (en) | 2004-11-19 |
| PT1394173E (pt) | 2007-11-28 |
| JP5153048B2 (ja) | 2013-02-27 |
| SI1829886T1 (sl) | 2015-12-31 |
| CN1495192A (zh) | 2004-05-12 |
| EP1829886B1 (de) | 2015-07-29 |
| JP5310059B2 (ja) | 2013-10-09 |
| AU2003236461A1 (en) | 2004-03-18 |
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| Date | Code | Title | Description |
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| UEP | Publication of translation of european patent specification |
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