ATE374814T1 - Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem - Google Patents

Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem

Info

Publication number
ATE374814T1
ATE374814T1 AT02722815T AT02722815T ATE374814T1 AT E374814 T1 ATE374814 T1 AT E374814T1 AT 02722815 T AT02722815 T AT 02722815T AT 02722815 T AT02722815 T AT 02722815T AT E374814 T1 ATE374814 T1 AT E374814T1
Authority
AT
Austria
Prior art keywords
protein synthesis
protein
cell
detergent
free
Prior art date
Application number
AT02722815T
Other languages
English (en)
Inventor
Kaori Tajima
Takanori Kigawa
Mikako Shirouzu
Takashi Yabuki
Goushi Ishihara
Shigeyuki Yokoyama
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Application granted granted Critical
Publication of ATE374814T1 publication Critical patent/ATE374814T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT02722815T 2001-05-02 2002-04-26 Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem ATE374814T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001135111 2001-05-02
JP2001285145A JP4838957B2 (ja) 2001-05-02 2001-09-19 無細胞タンパク質合成系を用いたタンパク質の製造方法

Publications (1)

Publication Number Publication Date
ATE374814T1 true ATE374814T1 (de) 2007-10-15

Family

ID=26614632

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02722815T ATE374814T1 (de) 2001-05-02 2002-04-26 Proteinsyntheseverfahren mit zellfreiem proteinsynthesesystem

Country Status (7)

Country Link
US (1) US7045593B2 (de)
EP (1) EP1384778B1 (de)
JP (1) JP4838957B2 (de)
AT (1) ATE374814T1 (de)
CA (1) CA2446031A1 (de)
DE (1) DE60222759T2 (de)
WO (1) WO2002090537A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003097829A1 (fr) * 2002-05-22 2003-11-27 Riken Procede de production d'une proteine dans un systeme de synthese de proteine sans cellule utilisant un vecteur d'expression de proteine fusionnee de thioredoxine
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP2008514240A (ja) * 2004-10-01 2008-05-08 インヴィトロジェン コーポレーション 生体分子のインビトロ合成用の供給バッファー、系、及び方法
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
JP4785174B2 (ja) * 2004-12-10 2011-10-05 株式会社豊田中央研究所 膜タンパク質複合材料及びその製造方法
US20060216762A1 (en) * 2005-03-24 2006-09-28 Bayer Healthcare Llc Extracting reagent for hydrophobic analyte in whole blood
JP4854415B2 (ja) * 2006-07-25 2012-01-18 花王株式会社 界面活性剤によるプロテアーゼ活性化制御
JP5704677B2 (ja) * 2007-11-05 2015-04-22 独立行政法人理化学研究所 膜タンパク質の製造方法
JP2010132594A (ja) * 2008-12-04 2010-06-17 Tokyo Institute Of Technology 膜タンパク質の固定化方法
JP6706813B2 (ja) 2015-06-30 2020-06-10 住友ゴム工業株式会社 膜結合蛋白質を結合させたゴム粒子の製造方法、空気入りタイヤの製造方法及びゴム製品の製造方法
JP6778372B2 (ja) 2015-06-30 2020-11-04 住友ゴム工業株式会社 ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法
JP6586693B2 (ja) 2015-06-30 2019-10-09 住友ゴム工業株式会社 ポリイソプレノイドの製造方法、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法
CA3032514C (en) * 2015-07-31 2021-05-04 Takehiro SHINODA Method of producing membrane protein and utilization thereof
JP6977928B2 (ja) 2016-12-21 2021-12-08 住友ゴム工業株式会社 トランス型ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法
JP7799272B2 (ja) 2022-03-02 2026-01-15 住友ゴム工業株式会社 変異シス型プレニルトランスフェラーゼ（ｃｐｔ）ファミリー蛋白質、ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法
JP2023178884A (ja) * 2022-06-06 2023-12-18 国立大学法人千葉大学 膜タンパク質のＣ末端に付加したＨｉｓ－ｔａｇに対する高親和性モノクローナル抗体

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270181A (en) 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
JP3092904B2 (ja) 1995-10-13 2000-09-25 株式会社エイチ・エス・ピー研究所 細菌により可溶性蛋白質を生産する方法
ES2230701T3 (es) * 1997-07-07 2005-05-01 Medical Research Council Procedimiento de seleccion in vitro.
JP2000175695A (ja) 1998-12-14 2000-06-27 Inst Of Physical & Chemical Res 無細胞タンパク質合成系によるポリペプチドの製造方法
ATE424467T1 (de) * 1999-05-11 2009-03-15 Cellfree Sciences Co Ltd Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins
US7276355B2 (en) * 2000-12-26 2007-10-02 Sekisui Chemical Co., Ltd. Process for production of a recombinant protein and a fusion protein
JP2002262873A (ja) 2001-03-06 2002-09-17 Inst Of Physical & Chemical Res タンパク質ドメインの製造方法及びその方法により得られたドメインを用いるタンパク質の立体構造解析方法

Also Published As

Publication number Publication date
EP1384778A1 (de) 2004-01-28
JP2003018999A (ja) 2003-01-21
CA2446031A1 (en) 2002-11-14
JP4838957B2 (ja) 2011-12-14
EP1384778B1 (de) 2007-10-03
WO2002090537A1 (en) 2002-11-14
DE60222759T2 (de) 2008-07-03
US20040203091A1 (en) 2004-10-14
DE60222759D1 (de) 2007-11-15
EP1384778A4 (de) 2004-06-16
US7045593B2 (en) 2006-05-16

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