ATE374825T1 - Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen - Google Patents

Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen

Info

Publication number
ATE374825T1
ATE374825T1 AT04713540T AT04713540T ATE374825T1 AT E374825 T1 ATE374825 T1 AT E374825T1 AT 04713540 T AT04713540 T AT 04713540T AT 04713540 T AT04713540 T AT 04713540T AT E374825 T1 ATE374825 T1 AT E374825T1
Authority
AT
Austria
Prior art keywords
fusion proteins
caspases
recombinant fusion
produce mature
mature recombinant
Prior art date
Application number
AT04713540T
Other languages
English (en)
Inventor
Nico Mertens
Andrew Kelly
Original Assignee
Biotecnol Sa
Vlaams Interuniv Inst Biotech
Univ Gent
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotecnol Sa, Vlaams Interuniv Inst Biotech, Univ Gent filed Critical Biotecnol Sa
Application granted granted Critical
Publication of ATE374825T1 publication Critical patent/ATE374825T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5415—Leukaemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04713540T 2003-02-21 2004-02-23 Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen ATE374825T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03447034 2003-02-21

Publications (1)

Publication Number Publication Date
ATE374825T1 true ATE374825T1 (de) 2007-10-15

Family

ID=32893040

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04713540T ATE374825T1 (de) 2003-02-21 2004-02-23 Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen

Country Status (13)

Country Link
US (1) US20060099689A1 (de)
EP (1) EP1597369B1 (de)
JP (1) JP2006518207A (de)
AT (1) ATE374825T1 (de)
AU (1) AU2004213586B2 (de)
CA (1) CA2511855A1 (de)
DE (1) DE602004009292T2 (de)
DK (1) DK1597369T3 (de)
NO (1) NO20053033L (de)
NZ (1) NZ541551A (de)
PT (1) PT1597369E (de)
WO (1) WO2004074488A1 (de)
ZA (1) ZA200504958B (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101412994B (zh) 2003-04-23 2012-05-30 弗·哈夫曼-拉罗切有限公司 使用粒酶b蛋白酶切割融合蛋白
JP2007512008A (ja) * 2003-11-24 2007-05-17 ノボ　ノルディスク　アクティーゼルスカブ 融合タンパク質及び該タンパク質の切断方法
CN102712914A (zh) 2009-09-29 2012-10-03 根特大学 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖
MX2012006985A (es) * 2009-12-16 2012-09-12 Allergan Inc Toxinas clostridium modificadas que comprenden un dominio de enlace al sitio de escision de la proteasa integrado.
KR101189193B1 (ko) * 2010-06-23 2012-10-09 한국과학기술연구원 저분자열충격단백질이 포함된 융합 단백질, 이에 의해 구성된 케이지 단백질 및 이의 신규한 용도
WO2019126960A1 (zh) * 2017-12-25 2019-07-04 清华大学 一种具有蛋白酶活性的多肽及其应用
KR20200080749A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법
US20220380739A1 (en) 2019-08-14 2022-12-01 Boehringer Ingelheim Rcv Gmbh & Co Kg Caspase-2 variants
JPWO2021193566A1 (de) * 2020-03-23 2021-09-30
EP4000596A1 (de) * 2020-11-17 2022-05-25 The Boots Company plc Tetrapeptid und zusammensetzungen mit tetrapeptiden
EP4000595A1 (de) * 2020-11-17 2022-05-25 The Boots Company plc Tetrapeptid und zusammensetzungen mit tetrapeptiden

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DE2900806C3 (de) * 1979-01-11 1982-03-18 B. Braun Melsungen Ag, 3508 Melsungen Vorrichtung zum Sammeln einer Körperflüssigkeit, insbesondere Urin
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
US5087564A (en) * 1985-06-20 1992-02-11 Monsanto Company Release of recombinant peptides from polypeptides using V8 endopeptidase
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
EP0286239B1 (de) * 1987-03-10 1996-03-13 New England Biolabs, Inc. Herstellung und Reinigung eines Proteins, das mit einem Bindungsprotein fusioniert ist
WO1990006369A1 (en) * 1988-12-09 1990-06-14 Smithkline Beecham Corporation Use of hiv protease cleavage site to express mature proteins
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
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Also Published As

Publication number Publication date
AU2004213586A1 (en) 2004-09-02
EP1597369B1 (de) 2007-10-03
NO20053033D0 (no) 2005-06-20
JP2006518207A (ja) 2006-08-10
NO20053033L (no) 2005-06-20
ZA200504958B (en) 2006-04-26
EP1597369A1 (de) 2005-11-23
NZ541551A (en) 2007-02-23
CA2511855A1 (en) 2004-09-02
DE602004009292D1 (de) 2007-11-15
DK1597369T3 (da) 2008-02-04
AU2004213586B2 (en) 2009-01-15
WO2004074488A1 (en) 2004-09-02
DE602004009292T2 (de) 2009-03-19
US20060099689A1 (en) 2006-05-11
PT1597369E (pt) 2008-01-10

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