NO20053033L - Anvendelse av caspase enzymer for modning av konstruerte rekombinante polypeptidfusjoner. - Google Patents

Anvendelse av caspase enzymer for modning av konstruerte rekombinante polypeptidfusjoner.

Info

Publication number
NO20053033L
NO20053033L NO20053033A NO20053033A NO20053033L NO 20053033 L NO20053033 L NO 20053033L NO 20053033 A NO20053033 A NO 20053033A NO 20053033 A NO20053033 A NO 20053033A NO 20053033 L NO20053033 L NO 20053033L
Authority
NO
Norway
Prior art keywords
maturation
caspase
recombinant polypeptide
engineered recombinant
polypeptide fusions
Prior art date
Application number
NO20053033A
Other languages
English (en)
Norwegian (no)
Other versions
NO20053033D0 (no
Inventor
Andrew Graham Kelly
Nico Maurice August Co Mertens
Original Assignee
Vlaams Interuniv Inst Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vlaams Interuniv Inst Biotech filed Critical Vlaams Interuniv Inst Biotech
Publication of NO20053033D0 publication Critical patent/NO20053033D0/no
Publication of NO20053033L publication Critical patent/NO20053033L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5415—Leukaemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
NO20053033A 2003-02-21 2005-06-20 Anvendelse av caspase enzymer for modning av konstruerte rekombinante polypeptidfusjoner. NO20053033L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03447034 2003-02-21
PCT/EP2004/001759 WO2004074488A1 (en) 2003-02-21 2004-02-23 Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions

Publications (2)

Publication Number Publication Date
NO20053033D0 NO20053033D0 (no) 2005-06-20
NO20053033L true NO20053033L (no) 2005-06-20

Family

ID=32893040

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20053033A NO20053033L (no) 2003-02-21 2005-06-20 Anvendelse av caspase enzymer for modning av konstruerte rekombinante polypeptidfusjoner.

Country Status (13)

Country Link
US (1) US20060099689A1 (de)
EP (1) EP1597369B1 (de)
JP (1) JP2006518207A (de)
AT (1) ATE374825T1 (de)
AU (1) AU2004213586B2 (de)
CA (1) CA2511855A1 (de)
DE (1) DE602004009292T2 (de)
DK (1) DK1597369T3 (de)
NO (1) NO20053033L (de)
NZ (1) NZ541551A (de)
PT (1) PT1597369E (de)
WO (1) WO2004074488A1 (de)
ZA (1) ZA200504958B (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2427974T3 (es) 2003-04-23 2013-11-05 F. Hoffmann-La Roche Ag Corte de proteínas de fusión mediante la utilización de la proteasa granzima B
CA2545024A1 (en) * 2003-11-24 2005-06-02 Novo Nordisk A/S Fusion proteins and methods of cleavage of such proteins
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
JP2013514091A (ja) * 2009-12-16 2013-04-25 アラーガン、インコーポレイテッド 組込型プロテアーゼ切断部位結合ドメインを含む改変クロストリジウム毒素
KR101189193B1 (ko) * 2010-06-23 2012-10-09 한국과학기술연구원 저분자열충격단백질이 포함된 융합 단백질, 이에 의해 구성된 케이지 단백질 및 이의 신규한 용도
WO2019126960A1 (zh) * 2017-12-25 2019-07-04 清华大学 一种具有蛋白酶活性的多肽及其应用
KR20200080749A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법
KR20220061126A (ko) 2019-08-14 2022-05-12 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 카스파제-2 변이체
JPWO2021193566A1 (de) * 2020-03-23 2021-09-30
EP4000596A1 (de) * 2020-11-17 2022-05-25 The Boots Company plc Tetrapeptid und zusammensetzungen mit tetrapeptiden
EP4000595A1 (de) * 2020-11-17 2022-05-25 The Boots Company plc Tetrapeptid und zusammensetzungen mit tetrapeptiden

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DE2900806C3 (de) * 1979-01-11 1982-03-18 B. Braun Melsungen Ag, 3508 Melsungen Vorrichtung zum Sammeln einer Körperflüssigkeit, insbesondere Urin
US5087564A (en) * 1985-06-20 1992-02-11 Monsanto Company Release of recombinant peptides from polypeptides using V8 endopeptidase
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
ATE135403T1 (de) * 1987-03-10 1996-03-15 New England Biolabs Inc Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
WO1990006369A1 (en) * 1988-12-09 1990-06-14 Smithkline Beecham Corporation Use of hiv protease cleavage site to express mature proteins
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
JP2001526533A (ja) * 1997-04-23 2001-12-18 フラームス・インテルウニフェルシタイル・インスティチュート・フォール・ビオテヒノロヒー λ様プロモーターを有する遺伝子の誘導性発現のための調節系
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
EP1013763B1 (de) * 1997-08-07 2011-02-02 Toray Industries, Inc. Methode zur herstellung von canine interleukin 18
US5989868A (en) * 1997-09-12 1999-11-23 The Board Of Regents Of The University Of Oklahoma Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in esherichia coli
US6379950B1 (en) * 1998-01-09 2002-04-30 Thomas Jefferson University Recombinant, active caspases and uses thereof
US6916917B1 (en) * 1998-11-17 2005-07-12 California Institute Of Technology Chimeric pro-caspases and methods of using same
IL129427A0 (en) * 1999-04-13 2000-02-17 Yeda Res & Dev Preparation of biologically active molecules
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
AU4906100A (en) * 1999-05-27 2000-12-18 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins
US7262005B1 (en) * 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
US6890745B1 (en) * 2000-07-19 2005-05-10 Chemicon International, Inc. Protease specific cleavable luciferases and methods of use thereof
US20020073441A1 (en) * 2000-12-13 2002-06-13 Ross Brian D. Compositions and methods for detecting proteolytic activity
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
CA2454238A1 (en) * 2001-07-31 2003-02-13 Clontech Laboratories, Inc. Method of detecting protease activity in a cell
US20030157519A1 (en) * 2001-10-15 2003-08-21 Stratagene Recombinant renilla reniformis system for bioluminescence resonance energy transfer

Also Published As

Publication number Publication date
EP1597369A1 (de) 2005-11-23
ATE374825T1 (de) 2007-10-15
PT1597369E (pt) 2008-01-10
DE602004009292D1 (de) 2007-11-15
NO20053033D0 (no) 2005-06-20
AU2004213586B2 (en) 2009-01-15
DE602004009292T2 (de) 2009-03-19
US20060099689A1 (en) 2006-05-11
CA2511855A1 (en) 2004-09-02
ZA200504958B (en) 2006-04-26
AU2004213586A1 (en) 2004-09-02
NZ541551A (en) 2007-02-23
EP1597369B1 (de) 2007-10-03
WO2004074488A1 (en) 2004-09-02
JP2006518207A (ja) 2006-08-10
DK1597369T3 (da) 2008-02-04

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