ATE382097T1 - Auf microarrays basierende substraktive hybridisierung - Google Patents

Auf microarrays basierende substraktive hybridisierung

Info

Publication number
ATE382097T1
ATE382097T1 AT00936091T AT00936091T ATE382097T1 AT E382097 T1 ATE382097 T1 AT E382097T1 AT 00936091 T AT00936091 T AT 00936091T AT 00936091 T AT00936091 T AT 00936091T AT E382097 T1 ATE382097 T1 AT E382097T1
Authority
AT
Austria
Prior art keywords
microarrays
identification
removal
nucleic acids
present
Prior art date
Application number
AT00936091T
Other languages
English (en)
Inventor
Steven Perrin
Katherine Call
Timothy Connolly
Original Assignee
Aventis Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Inc filed Critical Aventis Pharma Inc
Application granted granted Critical
Publication of ATE382097T1 publication Critical patent/ATE382097T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Ceramic Products (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT00936091T 1999-05-19 2000-05-19 Auf microarrays basierende substraktive hybridisierung ATE382097T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/314,698 US6638717B2 (en) 1999-05-19 1999-05-19 Microarray-based subtractive hybridzation

Publications (1)

Publication Number Publication Date
ATE382097T1 true ATE382097T1 (de) 2008-01-15

Family

ID=23221053

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00936091T ATE382097T1 (de) 1999-05-19 2000-05-19 Auf microarrays basierende substraktive hybridisierung

Country Status (18)

Country Link
US (1) US6638717B2 (de)
EP (1) EP1185699B1 (de)
JP (1) JP4714883B2 (de)
KR (1) KR100733930B1 (de)
AT (1) ATE382097T1 (de)
AU (1) AU779901B2 (de)
BR (1) BR0011297A (de)
CA (1) CA2372698C (de)
CY (1) CY1107428T1 (de)
DE (1) DE60037584T2 (de)
DK (1) DK1185699T3 (de)
ES (1) ES2296623T3 (de)
IL (2) IL146547A0 (de)
MX (1) MXPA01011730A (de)
NO (1) NO330813B1 (de)
PT (1) PT1185699E (de)
WO (1) WO2000070098A1 (de)
ZA (1) ZA200109609B (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050272056A1 (en) * 1996-11-13 2005-12-08 Deiss Louis P Gene identification method
US20020142341A1 (en) * 2001-03-28 2002-10-03 Makoto Kameyama Method and apparatus for producing probe carrier
US20040110153A1 (en) * 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification
JP4182338B2 (ja) * 2003-03-06 2008-11-19 信越化学工業株式会社 熱硬化性パーフルオロポリエーテル系ゴム組成物及びゴム製品
US7300755B1 (en) * 2003-05-12 2007-11-27 Fred Hutchinson Cancer Research Center Methods for haplotyping genomic DNA
US20050075728A1 (en) 2003-10-06 2005-04-07 Nguyen Tuoc Tan Minimally invasive valve replacement system
EP2290073A3 (de) 2004-05-28 2011-08-31 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
EP2302055B1 (de) 2004-11-12 2014-08-27 Asuragen, Inc. Verfahren und Zusammensetzungen, die miRNAs und miRNAa-inhibitorischen Molekülen verbunden sind
US20090131348A1 (en) 2006-09-19 2009-05-21 Emmanuel Labourier Micrornas differentially expressed in pancreatic diseases and uses thereof
WO2008036776A2 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
GB0618514D0 (en) * 2006-09-20 2006-11-01 Univ Nottingham Trent Method of detecting interactions on a microarray using nuclear magnetic resonance
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
WO2009137807A2 (en) 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
JP5221248B2 (ja) * 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のcDNAクローンの含有率を低減させたcDNAライブラリーの作製方法
US20100331204A1 (en) * 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
EP2542678B1 (de) 2010-03-04 2017-04-12 InteRNA Technologies B.V. Mirna-molekül, das durch seine quelle definiert ist, und seine therapeutischen verwendungen bei emt assoziierten krebs
EP2591106A1 (de) 2010-07-06 2013-05-15 InteRNA Technologies B.V. Mirna und ihre diagnostischen und therapeutischen verwendungen für mit melanomen assoziierte erkrankungen oder leiden oder für mit dem aktivierten braf-pfad assoziierte erkrankungen oder leiden
BR112013012265A2 (pt) 2010-11-17 2016-08-02 Asuragen Inc mirnas como biomarcadores para distinguir neoplasmas de tireoide benignos de malignos
EP2474617A1 (de) 2011-01-11 2012-07-11 InteRNA Technologies BV MIR zur Behandlung von Neoangiogenese
EP2681566A2 (de) 2011-02-28 2014-01-08 University of Iowa Research Foundation Anti-müller-hormon-veränderungen in der schwangerschaft und vorhersage von schwangerschaftsnebenwirkungen und des geschlechts
MX2013012709A (es) 2011-05-02 2013-12-06 Pioneer Hi Bred Int Estrategia de analisis de arnm bacteriano para descubrir nuevas moleculas de acido nucleico codificante de pesticidas.
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
WO2013063519A1 (en) 2011-10-26 2013-05-02 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
WO2013063544A1 (en) 2011-10-27 2013-05-02 Asuragen, Inc. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
WO2014007623A1 (en) 2012-07-03 2014-01-09 Interna Technologies B.V. Diagnostic portfolio and its uses
WO2014055117A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
US9758835B2 (en) 2013-03-15 2017-09-12 Baylor Research Institute Ulcerative colitis (UC)-associated colorectal neoplasia markers
US9944992B2 (en) 2013-03-15 2018-04-17 The University Of Chicago Methods and compositions related to T-cell activity
KR101516976B1 (ko) * 2013-10-30 2015-05-04 에스케이텔레콤 주식회사 표적 염기 서열 해독에서의 바이어스 제거 방법
CN111566212A (zh) 2017-11-03 2020-08-21 因特尔纳技术有限公司 miRNA分子,等同物,安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生
WO2019104155A2 (en) 2017-11-22 2019-05-31 The University Of Chicago Chemical probe-dependent evaluation of protein activity and uses thereof
EP3947727A4 (de) 2019-04-05 2023-01-04 Board of Regents, The University of Texas System Verfahren und anwendungen zur zellen-barcodierung
JP7566775B2 (ja) 2019-04-12 2024-10-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 筋肉量及び酸化的代謝を増加させるための組成物及び方法
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders
WO2025248505A1 (en) 2024-05-31 2025-12-04 Wayne State University Methods for treating endometrial and ovarian hyperproliferative disorders

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5591575A (en) 1993-04-07 1997-01-07 Amersham International Plc Subtraction hybridization employing aziridinylbenoquinone cross-linking agents
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5728529A (en) 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US5851772A (en) 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
US5712127A (en) 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
US5827658A (en) 1996-08-09 1998-10-27 The United States Of America As Reprsented By The Department Of Health And Human Services Isolation of amplified genes via cDNA subtractive hybridization
GB9618050D0 (en) 1996-08-29 1996-10-09 Cancer Res Campaign Tech Global amplification of nucleic acids
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
US5953727A (en) * 1996-10-10 1999-09-14 Incyte Pharmaceuticals, Inc. Project-based full-length biomolecular sequence database
WO1998043088A1 (en) 1997-03-24 1998-10-01 Smithkline Beecham Corporation Method of producing a subtraction library
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
WO1999023256A1 (en) 1997-10-30 1999-05-14 Cold Spring Harbor Laboratory Probe arrays and methods of using probe arrays for distinguishing dna
US5882874A (en) 1998-02-27 1999-03-16 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display

Also Published As

Publication number Publication date
NO20015635D0 (no) 2001-11-19
WO2000070098A1 (en) 2000-11-23
CA2372698C (en) 2010-04-27
KR100733930B1 (ko) 2007-07-03
DE60037584D1 (de) 2008-02-07
KR20020008190A (ko) 2002-01-29
JP2002543856A (ja) 2002-12-24
DK1185699T3 (da) 2008-04-28
IL146547A0 (en) 2002-07-25
US6638717B2 (en) 2003-10-28
US20020051970A1 (en) 2002-05-02
MXPA01011730A (es) 2002-04-24
CA2372698A1 (en) 2000-11-23
CY1107428T1 (el) 2012-12-19
JP4714883B2 (ja) 2011-06-29
IL146547A (en) 2008-04-13
EP1185699A1 (de) 2002-03-13
PT1185699E (pt) 2008-02-26
DE60037584T2 (de) 2009-01-08
NO20015635L (no) 2002-01-15
NO330813B1 (no) 2011-07-25
EP1185699B1 (de) 2007-12-26
ZA200109609B (en) 2003-04-30
AU779901B2 (en) 2005-02-17
AU5145500A (en) 2000-12-05
BR0011297A (pt) 2002-02-26
ES2296623T3 (es) 2008-05-01

Similar Documents

Publication Publication Date Title
ATE382097T1 (de) Auf microarrays basierende substraktive hybridisierung
Zhang et al. Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells
Fu et al. Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations
Li et al. RASL‐seq for massively parallel and quantitative analysis of gene expression
Guo et al. Four-color DNA sequencing with 3′-O-modified nucleotide reversible terminators and chemically cleavable fluorescent dideoxynucleotides
Akintunde et al. The evolution of next-generation sequencing technologies
Oberley et al. High-throughput screening of chromatin immunoprecipitates using CpG-island microarrays
Simon et al. Short-read sequencing technologies for transcriptional analyses
US5780231A (en) DNA extension and analysis with rolling primers
Raine et al. SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
Matz et al. Different strategies of differential display: areas of application
ATE343586T1 (de) Verfahren zur verwendung einer universellen bibliothek von peptid-nukleinsäuren zur optimierung der dns-hybridisierung
US5962228A (en) DNA extension and analysis with rolling primers
Bai et al. Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry
Hirst et al. Next generation sequencing based approaches to epigenomics
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
WO2000040755A3 (en) Method for accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
ATE322554T1 (de) Dns charakterisierung
Myllykangas et al. Overview of sequencing technology platforms
ATE403747T1 (de) Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten
GB2401433A (en) Methods of parallel gene cloning and analysis
Marinov On the design and prospects of direct RNA sequencing
Lovett The applications of single-cell genomics
JP2001521398A (ja) 性状検査用dna
Higgs et al. Using genomics to study how chromatin influences gene expression

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1185699

Country of ref document: EP

EEIH Change in the person of patent owner