ATE398181T1 - Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces - Google Patents

Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

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Publication number
ATE398181T1
ATE398181T1 AT98928202T AT98928202T ATE398181T1 AT E398181 T1 ATE398181 T1 AT E398181T1 AT 98928202 T AT98928202 T AT 98928202T AT 98928202 T AT98928202 T AT 98928202T AT E398181 T1 ATE398181 T1 AT E398181T1
Authority
AT
Austria
Prior art keywords
desired gene
copies
ribosomal rna
yeast cells
kluyveromyces
Prior art date
Application number
AT98928202T
Other languages
English (en)
Inventor
Bart Swinkels
OOIJEN Albert VAN
Der Haak Adria Noordermeer-Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE398181T1 publication Critical patent/ATE398181T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT98928202T 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces ATE398181T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97201053 1997-04-11
EP97201637 1997-06-03

Publications (1)

Publication Number Publication Date
ATE398181T1 true ATE398181T1 (de) 2008-07-15

Family

ID=26146351

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98928202T ATE398181T1 (de) 1997-04-11 1998-04-14 Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces

Country Status (8)

Country Link
EP (1) EP0973918B1 (de)
JP (1) JP4531868B2 (de)
AT (1) ATE398181T1 (de)
AU (1) AU8013798A (de)
DE (1) DE69839602D1 (de)
DK (1) DK0973918T3 (de)
ES (1) ES2308805T3 (de)
WO (1) WO1998046774A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3293264A1 (de) 2009-07-22 2018-03-14 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
CA3056920A1 (en) 2017-03-29 2018-10-04 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59501572A (ja) * 1982-05-19 1984-09-06 ユニリーバー ナームローゼ ベンノートシヤープ クルイベロミセス属酵母およびその製造法
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
DE69432543T2 (de) * 1993-07-23 2003-12-24 Dsm N.V., Te Heerlen Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

Also Published As

Publication number Publication date
WO1998046774A1 (en) 1998-10-22
EP0973918B1 (de) 2008-06-11
DE69839602D1 (de) 2008-07-24
ES2308805T3 (es) 2008-12-01
JP2002514077A (ja) 2002-05-14
JP4531868B2 (ja) 2010-08-25
EP0973918A1 (de) 2000-01-26
DK0973918T3 (da) 2008-09-22
AU8013798A (en) 1998-11-11

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ATE398181T1 (de) Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces
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