ATE403719T1 - Konditionelles selex - Google Patents

Konditionelles selex

Info

Publication number
ATE403719T1
ATE403719T1 AT00947548T AT00947548T ATE403719T1 AT E403719 T1 ATE403719 T1 AT E403719T1 AT 00947548 T AT00947548 T AT 00947548T AT 00947548 T AT00947548 T AT 00947548T AT E403719 T1 ATE403719 T1 AT E403719T1
Authority
AT
Austria
Prior art keywords
selex
conditional
nucleic acid
signal
target molecule
Prior art date
Application number
AT00947548T
Other languages
English (en)
Inventor
Jonathan Smith
Larry Gold
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somalogic Inc filed Critical Somalogic Inc
Application granted granted Critical
Publication of ATE403719T1 publication Critical patent/ATE403719T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Glass Compositions (AREA)
  • Prostheses (AREA)
  • Polyesters Or Polycarbonates (AREA)
AT00947548T 1999-07-29 2000-07-19 Konditionelles selex ATE403719T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/364,541 US6506887B1 (en) 1999-07-29 1999-07-29 Conditional-selex

Publications (1)

Publication Number Publication Date
ATE403719T1 true ATE403719T1 (de) 2008-08-15

Family

ID=23434946

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00947548T ATE403719T1 (de) 1999-07-29 2000-07-19 Konditionelles selex

Country Status (9)

Country Link
US (2) US6506887B1 (de)
EP (1) EP1203006B1 (de)
JP (1) JP4565787B2 (de)
AT (1) ATE403719T1 (de)
AU (1) AU6113200A (de)
CA (1) CA2380395C (de)
DE (1) DE60039765D1 (de)
DK (1) DK1203006T3 (de)
WO (1) WO2001009158A1 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE516366T1 (de) 2002-07-25 2011-07-15 Archemix Corp Regulierte aptamer-therapeutika
US9303262B2 (en) 2002-09-17 2016-04-05 Archemix Llc Methods for identifying aptamer regulators
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
US20050106594A1 (en) * 2003-08-22 2005-05-19 Andrew Ellington In vitro selection of aptamer beacons
WO2005051174A2 (en) * 2003-11-21 2005-06-09 The Trustees Of Columbia University In The City Of New York Nucleic acid aptamer-based compositions and methods
EP1700912B1 (de) * 2003-11-22 2014-10-29 Techno Medica Co., Ltd. Verfahren zum nachweis eines zielmoleküls unter verwendung eines aptamers
JP2007525661A (ja) * 2003-12-12 2007-09-06 セントルイス ユニバーシティー 大分子その他の分析物の検出用生物センサー
US7207206B2 (en) 2004-02-19 2007-04-24 Ut-Battelle, Llc Chemically-functionalized microcantilevers for detection of chemical, biological and explosive material
WO2007001448A2 (en) 2004-11-04 2007-01-04 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (de) 2005-06-10 2012-07-04 Saint Louis University Verfahren zur selektion von aptameren
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
WO2007070682A2 (en) * 2005-12-15 2007-06-21 Massachusetts Institute Of Technology System for screening particles
CA2648099C (en) * 2006-03-31 2012-05-29 The Brigham And Women's Hospital, Inc System for targeted delivery of therapeutic agents
JP5630998B2 (ja) 2006-05-15 2014-11-26 マサチューセッツ インスティテュート オブ テクノロジー 機能的粒子のためのポリマー
WO2007150030A2 (en) 2006-06-23 2007-12-27 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
WO2008019142A2 (en) * 2006-08-04 2008-02-14 Massachusetts Institute Of Technology Oligonucleotide systems for targeted intracellular delivery
US9725713B1 (en) 2007-01-22 2017-08-08 Steven A Benner In vitro selection with expanded genetic alphabets
US8586303B1 (en) 2007-01-22 2013-11-19 Steven Albert Benner In vitro selection with expanded genetic alphabets
EP2134830A2 (de) * 2007-02-09 2009-12-23 Massachusetts Institute of Technology Oszillations-zellkultur-bioreaktor
JP2010523595A (ja) 2007-04-04 2010-07-15 マサチューセッツ インスティテュート オブ テクノロジー ポリ（アミノ酸）ターゲッティング部分
WO2008124634A1 (en) 2007-04-04 2008-10-16 Massachusetts Institute Of Technology Polymer-encapsulated reverse micelles
JP2011500569A (ja) 2007-10-12 2011-01-06 マサチューセッツ インスティテュート オブ テクノロジー ワクチンナノテクノロジー
US8343497B2 (en) 2008-10-12 2013-01-01 The Brigham And Women's Hospital, Inc. Targeting of antigen presenting cells with immunonanotherapeutics
US8343498B2 (en) 2008-10-12 2013-01-01 Massachusetts Institute Of Technology Adjuvant incorporation in immunonanotherapeutics
US8591905B2 (en) 2008-10-12 2013-11-26 The Brigham And Women's Hospital, Inc. Nicotine immunonanotherapeutics
US8277812B2 (en) * 2008-10-12 2012-10-02 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IgG humoral response without T-cell antigen
US8993245B2 (en) 2008-11-21 2015-03-31 Mediomics, Llc Biosensor for detecting multiple epitopes on a target
US8236570B2 (en) * 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
WO2011100561A1 (en) 2010-02-12 2011-08-18 Saint Louis University Molecular biosensors capable of signal amplification
CN112587671A (zh) 2012-07-18 2021-04-02 博笛生物科技有限公司 癌症的靶向免疫治疗
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
EP4170031A1 (de) 2012-10-23 2023-04-26 Caris Science, Inc. Aptamere und verwendungen davon
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
DK3092256T3 (da) 2014-01-10 2022-06-20 Birdie Biopharmaceuticals Inc Forbindelser og sammensætninger til immunterapi
CN121243402A (zh) 2014-07-09 2026-01-02 博笛生物科技有限公司 用于治疗肿瘤的抗-pd-l1组合
CN105233291A (zh) 2014-07-09 2016-01-13 博笛生物科技有限公司 用于治疗癌症的联合治疗组合物和联合治疗方法
CN112587672A (zh) 2014-09-01 2021-04-02 博笛生物科技有限公司 用于治疗肿瘤的抗-pd-l1结合物
WO2016040830A1 (en) 2014-09-12 2016-03-17 Mediomics, Llc Molecular biosensors with a modular design
US20180153884A1 (en) 2015-05-31 2018-06-07 Curegenix Corporation Combination compositions for immunotherapy
WO2017035666A1 (en) * 2015-09-04 2017-03-09 Neoventures Biotechnology Inc. Method for the selection of aptamers for unbound targets
JP2019531351A (ja) 2016-10-14 2019-10-31 シーエヌエス バイオサイエンシーズ，インコーポレイティド 脊髄損傷者における神経障害性疼痛の治療
EP3332812A1 (de) 2016-12-07 2018-06-13 Rheinische Friedrich-Wilhelms-Universität Bonn Nukleinsäurebasierte anordnung und verwendung der anordnung in der krebstherapie
EP4225922A1 (de) 2020-10-07 2023-08-16 Centre National de la Recherche Scientifique (CNRS) Verfahren zur selektion von aptameren, riboschaltern und desoxyriboschaltern

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE229046T1 (de) 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
ES2259800T3 (es) * 1990-06-11 2006-10-16 Gilead Sciences, Inc. Procedimientos de uso de ligandos de acido nucleico.
US5789163A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5683867A (en) * 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
US5641629A (en) * 1990-06-11 1997-06-24 Nexstar Pharmacueticals Inc Spectroscopically detectable nucleic acid ligands
JPH06508022A (ja) 1991-02-21 1994-09-14 ギリアド　サイエンシズ，インコーポレイテッド 生体分子に特異的なアプタマーおよび生産方法
EP1067134B1 (de) * 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridisierung von mit Chromophoren und Fluorophoren konjugierten Polynukleotiden zur Erzeugung eines Donor-Donor Energietransfersystems
WO1994013791A1 (en) 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Regulatable nucleic acid therapeutic and methods of use thereof
US6214546B1 (en) 1995-02-27 2001-04-10 Intelligene Ltd. Detection of biomolecules
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
CA2264246A1 (en) 1996-08-26 1998-03-05 Intelligene Ltd. Catalytic nucleic acid and its medical use
WO1998027104A1 (en) 1996-12-19 1998-06-25 Yale University Bioreactive allosteric polynucleotides
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
JP4153068B2 (ja) * 1997-12-24 2008-09-17 浜松ホトニクス株式会社 蛍光免疫測定方法
IL126731A0 (en) 1998-10-23 1999-08-17 Intelligene Ltd A method of detection
EP1133513A4 (de) 1998-11-03 2002-07-03 Univ Yale Aus mehreren domänen von polynukleotiden bestehende molekulare sensoren
IL130606A0 (en) 1999-06-23 2000-06-01 Intelligene Ltd An array of nucleic acid sequence
IL132710A0 (en) 1999-11-02 2001-03-19 Intelligene Ltd Catalytic nucleic acid sequences
US20010046674A1 (en) 2000-02-03 2001-11-29 Andrew Ellington Signaling aptamers that transduce molecular recognition to a differential signal
EP1257639A2 (de) 2000-02-08 2002-11-20 Ribozyme Pharmaceuticals, Inc. Nukleozyme mit endonuklease aktivität
WO2001064956A2 (en) 2000-03-01 2001-09-07 Amgen Inc. The identification and use of effectors and allosteric molecules for the alteration of gene expression
EP1263947A2 (de) 2000-03-06 2002-12-11 Ribozyme Pharmaceuticals, Inc. Nukleinsäure sensormoleküle

Also Published As

Publication number Publication date
EP1203006A4 (de) 2005-01-12
DK1203006T3 (da) 2008-12-15
WO2001009158A1 (en) 2001-02-08
US20030087301A1 (en) 2003-05-08
JP4565787B2 (ja) 2010-10-20
AU6113200A (en) 2001-02-19
EP1203006B1 (de) 2008-08-06
US6506887B1 (en) 2003-01-14
CA2380395C (en) 2010-10-19
JP2003506670A (ja) 2003-02-18
DE60039765D1 (de) 2008-09-18
CA2380395A1 (en) 2001-02-08
EP1203006A1 (de) 2002-05-08
US6706482B2 (en) 2004-03-16

Similar Documents

Publication Publication Date Title
ATE403719T1 (de) Konditionelles selex
DE60322880D1 (de) Einfache katalytische dna-biosensoren für ionen auf grundlage von farbveränderungen
ATE330038T1 (de) Nukleinsäure-biosensor
DE69731226D1 (de) Verwendung von substrat-subtraktionsbibliotheken zur unterscheidung von enzymspezifitäten
DE60039986D1 (de) Nukleinsäureliganden von tenascin-c
DE60029092D1 (de) Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
ATE309536T1 (de) Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
ATE419392T1 (de) Verfahren zum nachweis von alkyliertem cytosin in dna
DE60231606D1 (de) Zirkuläre sonden amplifizierung (cpa) von zirkularisierten nucleinsäure-molekülen
ATE391188T1 (de) Cst (compartmentalised self tagging)
ATE130096T1 (de) Verfahren zur verstärkung des emissionssignals einer lumineszierenden verbindung.
CY1112525T1 (el) Βελτιωμενες μεθοδοι βεαμινg
EP1090294A4 (de) Verfahren um die anwesenheit von krebs in einer probe zu bestimmen durch nachweis der expression eines ssx-gens und peptiden, die von ssx oder ny-eso-1 abgeleitet sind
ATE460014T1 (de) System und verfahren zur aufwärtsstrecken- leistungsregelung
ATE437946T1 (de) Ribozyme mit breiter trna-aminoacylierungswirkung
DE60126311D1 (de) Verfahren zur 2d-inversion von doppel-laterolog-messungen
ATE365319T1 (de) Methode zur identifizierung von regulatoren der bildung von protein-age-derivaten
ATE486418T1 (de) Verbesserung von signalen
DE69635998D1 (de) Nachweisverfahren für verbindungen, welche die somatostatin- und insulinproduktion stimulieren
ATE221657T1 (de) Entstörung durch rheumafaktoren
ATE513040T1 (de) Zinkfingerdomänenbibliotheken
ATE447035T1 (de) Proteine beteiligt an signaltransduktion
DE60029429D1 (de) Verfahren und zusammensetzungen zur überwachung der modifikation von natürlichen bindungspartners
DE10256898A8 (de) Elektrochemischer Nachweis von Nukleinsäuren
DE60137721D1 (de) Inhibitoren von lyn-verbundener signaltransduktion (last) und deren verwendung zur behandlung von prostatakrebs

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties