ATE408681T1 - Verfahren zur reinigung einer rhizomucor aspartat-protease - Google Patents

Verfahren zur reinigung einer rhizomucor aspartat-protease

Info

Publication number
ATE408681T1
ATE408681T1 AT03755152T AT03755152T ATE408681T1 AT E408681 T1 ATE408681 T1 AT E408681T1 AT 03755152 T AT03755152 T AT 03755152T AT 03755152 T AT03755152 T AT 03755152T AT E408681 T1 ATE408681 T1 AT E408681T1
Authority
AT
Austria
Prior art keywords
microbial protease
separation medium
purifying
rhizomucor
aspartate protease
Prior art date
Application number
AT03755152T
Other languages
English (en)
Inventor
Van Beckhoven
Thierry Naeye
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE408681T1 publication Critical patent/ATE408681T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Detergent Compositions (AREA)
AT03755152T 2002-05-29 2003-05-28 Verfahren zur reinigung einer rhizomucor aspartat-protease ATE408681T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02100578 2002-05-29

Publications (1)

Publication Number Publication Date
ATE408681T1 true ATE408681T1 (de) 2008-10-15

Family

ID=29558402

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03755152T ATE408681T1 (de) 2002-05-29 2003-05-28 Verfahren zur reinigung einer rhizomucor aspartat-protease

Country Status (7)

Country Link
US (1) US7670821B2 (de)
EP (1) EP1507858B1 (de)
AT (1) ATE408681T1 (de)
AU (1) AU2003232845A1 (de)
DE (1) DE60323631D1 (de)
DK (1) DK1507858T3 (de)
WO (1) WO2003100048A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0004808D0 (sv) 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme
US20070215548A1 (en) * 2006-01-05 2007-09-20 Zhou Joe Xin H Systems and methods for packing chromatography columns
EA025412B1 (ru) 2006-04-13 2016-12-30 ДСМ АйПи АССЕТС Б.В. Жидкая композиция, содержащая аспарагиновую протеазу
CN102383555A (zh) * 2011-10-11 2012-03-21 常熟市华夏建筑节能材料有限公司 一种节能天花板
KR101743094B1 (ko) * 2015-05-13 2017-06-07 대한민국 신규 리조무코르 푸실루스 ｍ2559 균주 및 이의 용도
WO2017089613A1 (en) 2015-11-26 2017-06-01 Csk Food Enrichment B.V. Liquid composition comprising an aspartic protease having improved enzyme stability

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE280333C (de)
US2683682A (en) 1950-02-06 1954-07-13 Research Corp Differential inactivation of enzymes
DK110031A (de) 1965-12-02
US4086139A (en) 1976-04-09 1978-04-25 Gb Fermentation Industries Inc. Differential inactivation of amylase in amylase-protease mixtures
US4721673A (en) 1983-09-01 1988-01-26 Genex Corporation Recovery and activation process for microbially produced calf prochymosin
US4743551A (en) 1984-11-05 1988-05-10 Miles Inc. Purification of microbial rennet from Mucor miehei
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DD280333A1 (de) * 1989-03-02 1990-07-04 Adw Ddr Verfahren zur gewinnung von milchfaellenden enzymen
US5139943A (en) 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
DK165090D0 (da) 1990-07-09 1990-07-09 Kem En Tec As Konglomererede partikler
SE9101149D0 (sv) 1991-04-17 1991-04-17 Pharmacia Lkb Biotech Beads for down stream processing
AU653279B2 (en) * 1991-12-30 1994-09-22 Sanofi Novel 2-saccharinylmethyl heterocyclic carboxylates useful as proteolytic enzyme inhibitors and compositions and method of use thereof
DE4323913A1 (de) * 1993-04-03 1995-01-19 Merck Patent Gmbh Trennmaterialien für die hydrophobe Chromatographie
DK47493D0 (da) 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
US20040214157A1 (en) 1994-06-29 2004-10-28 Simon C. Burton Chromatographic resins and methods for using same
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
FR2737892B1 (fr) * 1995-08-17 1997-10-24 Fournier Ind & Sante Nouveaux composes de benzenesulfonamide, procede de preparation et utilisation en therapeutique
CN1203627A (zh) 1995-12-07 1998-12-30 诺沃挪第克公司 酶活性的选择性灭活
SE9700383D0 (sv) 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
WO1998058911A2 (en) * 1997-06-23 1998-12-30 Pfizer Inc. Prostaglandin agonists
ES2277910T3 (es) * 2000-02-08 2007-08-01 Upfront Chromatography A/S Nuevos materiales de resina y su uso para recuperar proteinas o peptidos.
SE0004808D0 (sv) * 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme

Also Published As

Publication number Publication date
DE60323631D1 (de) 2008-10-30
EP1507858B1 (de) 2008-09-17
EP1507858A1 (de) 2005-02-23
AU2003232845A1 (en) 2003-12-12
WO2003100048A1 (en) 2003-12-04
US7670821B2 (en) 2010-03-02
DK1507858T3 (da) 2009-02-02
US20050153418A1 (en) 2005-07-14

Similar Documents

Publication Publication Date Title
ATE448258T1 (de) Befestigung von molekülen auf oberflächen
DK1252328T3 (da) Fremgangsmåde og midler til isolering af mikroorganismer
ATE454463T1 (de) Überexpression von extremozymgenen in pseudomonas und nahe verwandte bakterien
ATE435280T1 (de) Methode zur isolierung von dna aus biologischen materialien
ATE196148T1 (de) Verfahren zur selektiver trennung organischer komponenten aus biologischen flüssigkeiten
DE602004026343D1 (de) Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
DE502004009871D1 (de) Verfahren zur modifikation von chiralen flüssigkri
FI20051248A7 (fi) Biologisten komponenttien rikastusyksikkö ja rikastusmenetelmä
ATE531691T1 (de) Verfahren zur reinigung von l-cystein
BRPI0509023A (pt) nitrila hidratase de rhodococcus
GB0411381D0 (en) Assay method and apparatus
ATE362530T1 (de) Verfahren und vorrichtung zur reinigung von nukleinsäuren
DE69739898D1 (de) Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten
ATE225362T1 (de) Verfahren zur reinigung kurzer nukleinsäuren
ATE408681T1 (de) Verfahren zur reinigung einer rhizomucor aspartat-protease
ATE263842T1 (de) Multiplex-sequenzierungsverfahren
SE0400490D0 (sv) Plasmid purification
ATE248631T1 (de) Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle
DE60219428D1 (de) Biochemische methode und apparat zur detektion genetischer charakteristika
ATE362896T1 (de) Verfahren zur reinigung von wasser
ATE340634T1 (de) Verfahren zur verringerung einer adsorptionsneigung von molekülen oder biologischen zellen an einer materialoberfläche
ATE420939T1 (de) Verfahren zur transalkylierung von aromatischen fluiden
ATE518963T1 (de) Evaneszenz-basierendes multiplex- sequenzierungsverfahren
ATE402198T1 (de) Verfahren zur immobilisierung biologischer moleküle an feste oberflächen
ATE386107T1 (de) Verfahren zur aufreinigung von chymosin

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties
REN Ceased due to non-payment of the annual fee