ATE42339T1 - Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung. - Google Patents

Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung.

Info

Publication number: ATE42339T1
Authority: AT; Austria
Prior art keywords: polypeptide; fusion protein; calcitonin; cat; manufacture
Prior art date: 1983-05-24

Application number

AT84303517T

Other languages

English (en)

Inventor

Alan David Bennett

Stephen Keith Rhind

Peter Anthony Lowe

Christopher Clive Ga Hentschel

Original Assignee

Celltech Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-05-24

Filing date

1984-05-24

Publication date

1989-05-15

1984-05-24 Application filed by Celltech Ltd filed Critical Celltech Ltd

1989-05-15 Application granted granted Critical

1989-05-15 Publication of ATE42339T1 publication Critical patent/ATE42339T1/de

Links

229920001184 polypeptide Polymers 0.000 title abstract 5
102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
238000004519 manufacturing process Methods 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
108090000623 proteins and genes Proteins 0.000 title abstract 2
102000004169 proteins and genes Human genes 0.000 title abstract 2
108020001507 fusion proteins Proteins 0.000 abstract 4
102000037865 fusion proteins Human genes 0.000 abstract 4
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 abstract 3
108090000746 Chymosin Proteins 0.000 abstract 2
230000000890 antigenic effect Effects 0.000 abstract 2
102000055006 Calcitonin Human genes 0.000 abstract 1
108060001064 Calcitonin Proteins 0.000 abstract 1
239000000055 Corticotropin-Releasing Hormone Substances 0.000 abstract 1
108090000790 Enzymes Proteins 0.000 abstract 1
102000004190 Enzymes Human genes 0.000 abstract 1
241000710198 Foot-and-mouth disease virus Species 0.000 abstract 1
239000004471 Glycine Substances 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
238000001042 affinity chromatography Methods 0.000 abstract 1
BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 abstract 1
229960004015 calcitonin Drugs 0.000 abstract 1
229940080701 chymosin Drugs 0.000 abstract 1
238000003776 cleavage reaction Methods 0.000 abstract 1
IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 abstract 1
229960000258 corticotropin Drugs 0.000 abstract 1
229940088598 enzyme Drugs 0.000 abstract 1
239000000122 growth hormone Substances 0.000 abstract 1
229940088597 hormone Drugs 0.000 abstract 1
239000005556 hormone Substances 0.000 abstract 1
230000002163 immunogen Effects 0.000 abstract 1
239000004026 insulin derivative Substances 0.000 abstract 1
GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical compound C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 abstract 1
108010064037 prorennin Proteins 0.000 abstract 1
230000007017 scission Effects 0.000 abstract 1
239000000126 substance Substances 0.000 abstract 1
239000000758 substrate Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- C07K14/695—Corticotropin [ACTH]
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Engineering & Computer Science (AREA)
Biophysics (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Epidemiology (AREA)
Biomedical Technology (AREA)
Endocrinology (AREA)
Immunology (AREA)
Mycology (AREA)
Pharmacology & Pharmacy (AREA)
Biotechnology (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Saccharide Compounds (AREA)

AT84303517T 1983-05-24 1984-05-24 Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung. ATE42339T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB838314362A GB8314362D0 (en)	1983-05-24	1983-05-24	Polypeptide and protein products
EP84303517A EP0131363B1 (de)	1983-05-24	1984-05-24	Polypeptid- und Proteinprodukte und Verfahren zu deren Herstellung und Verwendung

Publications (1)

Publication Number	Publication Date
ATE42339T1 true ATE42339T1 (de)	1989-05-15

Family

ID=10543274

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT84303517T ATE42339T1 (de)	1983-05-24	1984-05-24	Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung.

Country Status (8)

Country	Link
US (1)	US5093241A (de)
EP (1)	EP0131363B1 (de)
JP (1)	JPH0638755B2 (de)
AT (1)	ATE42339T1 (de)
CA (1)	CA1306207C (de)
DE (1)	DE3477810D1 (de)
GB (1)	GB8314362D0 (de)
WO (1)	WO1984004756A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
US4751180A (en) *	1985-03-28	1988-06-14	Chiron Corporation	Expression using fused genes providing for protein product
CA1260858A (en) *	1985-03-28	1989-09-26	Lawrence S. Cousens	Expression using fused genes providing for protein product
US5342921A (en) *	1985-03-28	1994-08-30	Chiron Corporation	Superoxide dismutase fusion polypeptides for expression of mammalian proteins
DE3686365T3 (de)	1985-06-20	2000-07-27	Fujisawa Pharmaceutical Co., Ltd.	Herstellungsverfahren für humanes atriales natriuretisches Polypeptid.
WO1987002385A1 (en) *	1985-10-11	1987-04-23	Genetics Institute, Inc.	Method for producing heterologous proteins
ES2043610T3 (es) *	1986-01-31	1994-01-01	Sagami Chem Res	Procedimiento para la produccion de un polipeptido hibrido del tipo activador de plasminogeno.
SE8601940D0 (sv) *	1986-04-25	1986-04-25	Kabigen Ab	Preparation of fused proteins, antibodies and processes therefor
US4859609A (en) *	1986-04-30	1989-08-22	Genentech, Inc.	Novel receptors for efficient determination of ligands and their antagonists or agonists
US5215896A (en) *	1987-03-20	1993-06-01	Creative Biomolecules, Inc.	Leader sequences for the production of recombinant proteins
US5013653A (en) *	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
FR2615528B1 (fr) *	1987-05-18	1991-05-24	Pasteur Institut	Procede de production d'un polypeptide ayant des proprietes choisies determinees, dans un organisme-hote transforme par un adn recombinant contenant des sequences nucleiques codant pour ce polypeptide et pour une proteine affine d'ose
DE3731875A1 (de) *	1987-09-18	1989-03-30	Schering Ag	Gentechnische herstellung von neukombinierten plasmiden fuer die produktion von humanem adrenocorticotropen horman (acth) und davon abgeleiteten derivaten durch enzymatische spaltung primaer synthetisierter fusionsproteine
JP2653820B2 (ja) *	1988-03-14	1997-09-17	壽之松尾	アミド化酵素及びその製造方法
AU4216689A (en) *	1988-08-11	1990-03-05	California Biotechnology, Inc.	Method for stabilizing heterologous protein expression and vectors for use therein
IL91462A0 (en) *	1988-08-30	1990-04-29	Applied Research Systems	Recombinant fusion proteins and dna encoding for such a protein
JP2852431B2 (ja) *	1988-12-07	1999-02-03	財団法人阪大微生物病研究会	レトロウイルスのプロテアーゼ、逆転写酵素及びエンドヌクレアーゼ、並びにこれ等の酵素の製法
AU5355790A (en) *	1989-04-19	1990-11-16	Cetus Corporation	Multifunctional m-csf proteins and genes encoding therefor
WO1991006319A1 (en) *	1989-10-27	1991-05-16	Arch Development Corporation	Methods and compositions for promoting immunopotentiation
HUT62654A (en) *	1990-05-28	1993-05-28	Univ Osaka Res Found	Process for producing plasmid capable of expressing retrovirus gene and processing it after translation, as well its expression products
US5595887A (en) *	1990-07-16	1997-01-21	Bionebraska, Inc.	Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US6316004B1 (en) *	1993-06-22	2001-11-13	T. Tikhonenko	Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals
AU4415796A (en) *	1994-12-07	1996-06-26	Bionebraska, Inc.	Production of c-terminal amidated peptides from recombinant protein constructs
US5962270A (en)	1996-02-06	1999-10-05	Bionebraska, Inc.	Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
DE69930938T2 (de) *	1998-07-10	2007-01-11	Scios Inc., Fremont	Verfahren zur herstellung eines peptids mit einem pi grösser als 8 oder kleiner als 5
US6818611B1 (en)	1998-10-13	2004-11-16	University Of Georgia Research Foundation, Inc.	Stabilized bioactive peptides and methods of identification, synthesis and use
US20030190740A1 (en)	1998-10-13	2003-10-09	The University Of Georgia Research Foundation, Inc	Stabilized bioactive peptides and methods of identification, synthesis, and use
US6461834B1 (en)	1998-11-06	2002-10-08	Bionebraska, Inc.	Clostripain catalyzed amidation of peptides
DK1035133T3 (da)	1999-02-17	2005-05-02	Pfizer Prod Inc	Fusionsproteiner, der omfatter bærere, som kan inducere et dobbelt immunrespons
AU2003243316A1 (en) *	2002-05-24	2003-12-12	Nps Allelix Corp.	Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides
CN101511387A (zh) *	2006-09-08	2009-08-19	惠氏公司	使用亲和色谱法纯化蛋白质的精氨酸洗涤液
AU2008246442B2 (en)	2007-05-04	2014-07-03	Technophage, Investigacao E Desenvolvimento Em Biotecnologia, Sa	Engineered rabbit antibody variable domains and uses thereof
AU2013296237B2 (en)	2012-08-03	2019-05-16	Foundation Medicine, Inc.	Human papilloma virus as predictor of cancer prognosis
US10227384B2 (en)	2013-04-12	2019-03-12	Yale University	Modified proteins and methods of use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4411994A (en) *	1978-06-08	1983-10-25	The President And Fellows Of Harvard College	Protein synthesis
CA1200773A (en) *	1980-02-29	1986-02-18	William J. Rutter	Expression linkers
WO1983000346A1 (en) *	1981-07-15	1983-02-03	Roger Kingdon Craig	Human calcitonin precursor polyprotein structural gene

1983
- 1983-05-24 GB GB838314362A patent/GB8314362D0/en active Pending
1984
- 1984-05-24 CA CA000455032A patent/CA1306207C/en not_active Expired - Lifetime
- 1984-05-24 AT AT84303517T patent/ATE42339T1/de not_active IP Right Cessation
- 1984-05-24 DE DE8484303517T patent/DE3477810D1/de not_active Expired
- 1984-05-24 WO PCT/GB1984/000179 patent/WO1984004756A1/en not_active Ceased
- 1984-05-24 JP JP50233884A patent/JPH0638755B2/ja not_active Expired - Lifetime
- 1984-05-24 EP EP84303517A patent/EP0131363B1/de not_active Expired
1988
- 1988-09-23 US US07/250,962 patent/US5093241A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0131363B1 (de)	1989-04-19
JPH0638755B2 (ja)	1994-05-25
US5093241A (en)	1992-03-03
JPS60501391A (ja)	1985-08-29
WO1984004756A1 (en)	1984-12-06
DE3477810D1 (en)	1989-05-24
GB8314362D0 (en)	1983-06-29
CA1306207C (en)	1992-08-11
EP0131363A1 (de)	1985-01-16

Legal Events

Date	Code	Title	Description
1989-09-15	UEP	Publication of translation of european patent specification
1995-02-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE42339T1 (de)	1989-05-15	Polypeptid- und proteinprodukte und verfahren zu deren herstellung und verwendung.
DE68922917D1 (de)	1995-07-13	KEX2-Endoprotease und Verfahren zu ihrer Herstellung.
ATE124456T1 (de)	1995-07-15	Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen.
DE3752284D1 (de)	1999-08-19	Hormon-rezeptor-verbindungen und methoden
BG38166A3 (bg)	1985-10-15	Метод за получаване на полипептид,включващ експресия на негов хетероложен ген от рекомбинантен микробен клониращ вектор
ES8600783A1 (es)	1985-11-01	Procedimiento para la preparacion de la hormona polipeptidica factor de crecimiento de nervios de humano.
DE3669175D1 (de)	1990-04-05	Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung.
EP0775750B1 (de)	2007-06-13	Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
ATE90944T1 (de)	1993-07-15	Fuer ein hirudinaehnliches protein kodierende dns- sequenz und verfahren zur herstellung eines solchen proteins.
ATE30246T1 (de)	1987-10-15	Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen.
ES2083352T3 (es)	1996-04-16	Produccion de proteinas activas que contienen residuos de cisteina.
DK383984D0 (da)	1984-08-09	Fremgangsmaade til fremstilling af sekretin
DE58909692D1 (de)	1996-07-25	Verfahren zur selektiven Spaltung von Fusionsproteinen
KR100312455B1 (ko)	2001-11-03	공유적 결합을 이용한 고정화 유로키나제의 제조방법
KR100289500B1 (ko)	2001-05-02	반공유적 결합을 이용한 고정화 유로키나제의 제조방법
FI97239C (fi)	1996-11-11	Menetelmä fuusioproteiinin valmistamiseksi
JPS6091987A (ja)	1985-05-23	特定の蛋白質の過剰生産を指示する発現プラスミド