ATE435304T1 - Verfahren zur in-vitro detektion und quantifizierung von hiv dna mittels quantitativer pcr - Google Patents
Verfahren zur in-vitro detektion und quantifizierung von hiv dna mittels quantitativer pcrInfo
- Publication number
- ATE435304T1 ATE435304T1 AT05777709T AT05777709T ATE435304T1 AT E435304 T1 ATE435304 T1 AT E435304T1 AT 05777709 T AT05777709 T AT 05777709T AT 05777709 T AT05777709 T AT 05777709T AT E435304 T1 ATE435304 T1 AT E435304T1
- Authority
- AT
- Austria
- Prior art keywords
- quantitative pcr
- hiv dna
- quantification
- hiv
- dna
- Prior art date
Links
- 238000003753 real-time PCR Methods 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000338 in vitro Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000011002 quantification Methods 0.000 title abstract 2
- 241000725303 Human immunodeficiency virus Species 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108020005202 Viral DNA Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 108700004026 gag Genes Proteins 0.000 abstract 1
- 101150098622 gag gene Proteins 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- AIDS & HIV (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200401986 | 2004-08-06 | ||
| PCT/EP2005/053384 WO2006013144A2 (en) | 2004-08-06 | 2005-07-14 | Method of in-vitro detection and quantification of hiv dna by quantitative pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE435304T1 true ATE435304T1 (de) | 2009-07-15 |
Family
ID=35517608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05777709T ATE435304T1 (de) | 2004-08-06 | 2005-07-14 | Verfahren zur in-vitro detektion und quantifizierung von hiv dna mittels quantitativer pcr |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20090208929A1 (de) |
| EP (1) | EP1711636B1 (de) |
| CN (1) | CN101035911A (de) |
| AT (1) | ATE435304T1 (de) |
| BR (1) | BRPI0514156A (de) |
| CA (1) | CA2573591A1 (de) |
| DE (1) | DE602005015189D1 (de) |
| ES (1) | ES2328944T3 (de) |
| RU (1) | RU2007108303A (de) |
| WO (1) | WO2006013144A2 (de) |
| ZA (1) | ZA200701945B (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5709897B2 (ja) * | 2009-12-24 | 2015-04-30 | シージーン アイエヌシー | 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出 |
| WO2012153153A1 (en) | 2011-05-11 | 2012-11-15 | Diagon Kft. | Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose |
| US20170044631A1 (en) * | 2014-04-14 | 2017-02-16 | The Unite States of America, as represented by the Secretary, Dept. of Health and Human Services | Methods for rapid detection and identification of viral nucleic acids |
| CN109371169B (zh) * | 2018-11-29 | 2022-02-11 | 中国人民解放军军事科学院军事医学研究院 | 一种用于辅助诊断早期hiv感染的试剂盒及其专用成套引物对 |
| CN120758675A (zh) * | 2025-07-04 | 2025-10-10 | 武汉潇正生物科技有限公司 | 快速定量检测整合hiv前病毒基因组的试剂盒及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0630973A3 (de) * | 1993-05-14 | 1995-04-26 | Eastman Kodak Co | Diagnosezusammensetzungen, Elemente, Verfahren und Testkits zur Amplifikation und zum Nachweis von zwei oder mehren DNS und Verwendung von Primern mit angepassten Schmelztemperaturen. |
| US6294326B1 (en) * | 1997-11-07 | 2001-09-25 | Abbott Laboratories | Analyte detection process using dual labeled probes |
| JP2000139500A (ja) * | 1998-11-17 | 2000-05-23 | Tokyoto Igaku Kenkyu Kiko | リアルタイム検出pcrによるhiv遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ |
-
2005
- 2005-07-14 AT AT05777709T patent/ATE435304T1/de not_active IP Right Cessation
- 2005-07-14 RU RU2007108303/13A patent/RU2007108303A/ru not_active Application Discontinuation
- 2005-07-14 US US11/632,248 patent/US20090208929A1/en not_active Abandoned
- 2005-07-14 DE DE602005015189T patent/DE602005015189D1/de not_active Expired - Fee Related
- 2005-07-14 ES ES05777709T patent/ES2328944T3/es not_active Expired - Lifetime
- 2005-07-14 EP EP05777709A patent/EP1711636B1/de not_active Expired - Lifetime
- 2005-07-14 CA CA002573591A patent/CA2573591A1/en not_active Abandoned
- 2005-07-14 BR BRPI0514156-7A patent/BRPI0514156A/pt not_active IP Right Cessation
- 2005-07-14 CN CNA2005800339359A patent/CN101035911A/zh active Pending
- 2005-07-14 WO PCT/EP2005/053384 patent/WO2006013144A2/en not_active Ceased
-
2007
- 2007-03-06 ZA ZA200701945A patent/ZA200701945B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1711636A2 (de) | 2006-10-18 |
| DE602005015189D1 (de) | 2009-08-13 |
| CA2573591A1 (en) | 2006-02-09 |
| EP1711636B1 (de) | 2009-07-01 |
| CN101035911A (zh) | 2007-09-12 |
| US20090208929A1 (en) | 2009-08-20 |
| RU2007108303A (ru) | 2008-09-20 |
| WO2006013144A3 (en) | 2006-06-01 |
| BRPI0514156A (pt) | 2008-06-03 |
| ZA200701945B (en) | 2008-07-30 |
| ES2328944T3 (es) | 2009-11-19 |
| WO2006013144A2 (en) | 2006-02-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |