BRPI0514156A - método de detecção e quantificação in vitro de dna de hiv através de pcr quantitativa - Google Patents
método de detecção e quantificação in vitro de dna de hiv através de pcr quantitativaInfo
- Publication number
- BRPI0514156A BRPI0514156A BRPI0514156-7A BRPI0514156A BRPI0514156A BR PI0514156 A BRPI0514156 A BR PI0514156A BR PI0514156 A BRPI0514156 A BR PI0514156A BR PI0514156 A BRPI0514156 A BR PI0514156A
- Authority
- BR
- Brazil
- Prior art keywords
- quantitative pcr
- hiv dna
- vitro
- hiv
- dna
- Prior art date
Links
- 238000003753 real-time PCR Methods 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000338 in vitro Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000011002 quantification Methods 0.000 title abstract 2
- 241000725303 Human immunodeficiency virus Species 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108020005202 Viral DNA Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 108700004026 gag Genes Proteins 0.000 abstract 1
- 101150098622 gag gene Proteins 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- AIDS & HIV (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
MéTODO DE DETECçãO E QUANTIFICAçãO IN VITRO DE DNA DE HIV ATRAVéS DE PCR QUANTITATIVA. A presente invenção refere-se a um processa para a detecção e quantificação in vitro de DNA de HIV através de PCR quantitativa. A invenção consiste no desenvolvimento de pares de oligonucleotídeos capazes de hibridizar com fragmentos da seqüência de gene gag presentes no genoma do vírus HIV. Esses oligonucleotídeos permitem a amplificação do DNA viral através de PCR quantitativa. Mesmo no caso de amostras com baixa carga viral, a invenção permite a detecção do DNA do vírus HIV através de justaposição ou de uma PCR convencional com uma PCR quantitativa, ou com uma PCR quantitativa aninhada dupla, usando pares de iniciadores diferentes em cada amplificação. A invenção, comparada com os métodos conhecidos, permite a determinação quantitativa e qualitativamente, in vitro, da presença de DNA de HIV em amostras, de uma maneira rápida, reproduzível e com alta sensibilidade.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200401986 | 2004-08-06 | ||
| PCT/EP2005/053384 WO2006013144A2 (en) | 2004-08-06 | 2005-07-14 | Method of in-vitro detection and quantification of hiv dna by quantitative pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0514156A true BRPI0514156A (pt) | 2008-06-03 |
Family
ID=35517608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0514156-7A BRPI0514156A (pt) | 2004-08-06 | 2005-07-14 | método de detecção e quantificação in vitro de dna de hiv através de pcr quantitativa |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20090208929A1 (pt) |
| EP (1) | EP1711636B1 (pt) |
| CN (1) | CN101035911A (pt) |
| AT (1) | ATE435304T1 (pt) |
| BR (1) | BRPI0514156A (pt) |
| CA (1) | CA2573591A1 (pt) |
| DE (1) | DE602005015189D1 (pt) |
| ES (1) | ES2328944T3 (pt) |
| RU (1) | RU2007108303A (pt) |
| WO (1) | WO2006013144A2 (pt) |
| ZA (1) | ZA200701945B (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011078439A1 (en) * | 2009-12-24 | 2011-06-30 | Seegene, Inc. | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
| EP2707496A1 (en) | 2011-05-11 | 2014-03-19 | Diagon Kft. | Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose |
| WO2015160536A1 (en) * | 2014-04-14 | 2015-10-22 | The United States Of America, As Represented By The Secretary Department Of Health And Human Services | Methods for rapid detection and identification of viral nucleic acids |
| CN109371169B (zh) * | 2018-11-29 | 2022-02-11 | 中国人民解放军军事科学院军事医学研究院 | 一种用于辅助诊断早期hiv感染的试剂盒及其专用成套引物对 |
| CN120758675A (zh) * | 2025-07-04 | 2025-10-10 | 武汉潇正生物科技有限公司 | 快速定量检测整合hiv前病毒基因组的试剂盒及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG63589A1 (en) * | 1993-05-14 | 1999-03-30 | Johnson & Johnson Clin Diag | Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures |
| US6294326B1 (en) * | 1997-11-07 | 2001-09-25 | Abbott Laboratories | Analyte detection process using dual labeled probes |
| JP2000139500A (ja) * | 1998-11-17 | 2000-05-23 | Tokyoto Igaku Kenkyu Kiko | リアルタイム検出ｐｃｒによるｈｉｖ遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ |
-
2005
- 2005-07-14 BR BRPI0514156-7A patent/BRPI0514156A/pt not_active IP Right Cessation
- 2005-07-14 ES ES05777709T patent/ES2328944T3/es not_active Expired - Lifetime
- 2005-07-14 WO PCT/EP2005/053384 patent/WO2006013144A2/en not_active Ceased
- 2005-07-14 EP EP05777709A patent/EP1711636B1/en not_active Expired - Lifetime
- 2005-07-14 DE DE602005015189T patent/DE602005015189D1/de not_active Expired - Fee Related
- 2005-07-14 CA CA002573591A patent/CA2573591A1/en not_active Abandoned
- 2005-07-14 AT AT05777709T patent/ATE435304T1/de not_active IP Right Cessation
- 2005-07-14 CN CNA2005800339359A patent/CN101035911A/zh active Pending
- 2005-07-14 US US11/632,248 patent/US20090208929A1/en not_active Abandoned
- 2005-07-14 RU RU2007108303/13A patent/RU2007108303A/ru not_active Application Discontinuation
-
2007
- 2007-03-06 ZA ZA200701945A patent/ZA200701945B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE435304T1 (de) | 2009-07-15 |
| ES2328944T3 (es) | 2009-11-19 |
| WO2006013144A2 (en) | 2006-02-09 |
| RU2007108303A (ru) | 2008-09-20 |
| CA2573591A1 (en) | 2006-02-09 |
| WO2006013144A3 (en) | 2006-06-01 |
| DE602005015189D1 (de) | 2009-08-13 |
| US20090208929A1 (en) | 2009-08-20 |
| ZA200701945B (en) | 2008-07-30 |
| EP1711636A2 (en) | 2006-10-18 |
| CN101035911A (zh) | 2007-09-12 |
| EP1711636B1 (en) | 2009-07-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2158 DE 15/05/2012. |