ATE447615T1 - Verfahren zur sekretion und produktion von protein - Google Patents

Verfahren zur sekretion und produktion von protein

Info

Publication number
ATE447615T1
ATE447615T1 AT02708684T AT02708684T ATE447615T1 AT E447615 T1 ATE447615 T1 AT E447615T1 AT 02708684 T AT02708684 T AT 02708684T AT 02708684 T AT02708684 T AT 02708684T AT E447615 T1 ATE447615 T1 AT E447615T1
Authority
AT
Austria
Prior art keywords
coryneform bacterium
heterologous protein
protein
production
sequence encoding
Prior art date
Application number
AT02708684T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Haruo Heima
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of ATE447615T1 publication Critical patent/ATE447615T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT02708684T 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein ATE447615T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001098808 2001-03-30
PCT/JP2002/002978 WO2002081694A1 (fr) 2001-03-30 2002-03-27 Procede de secretion et de production de proteine

Publications (1)

Publication Number Publication Date
ATE447615T1 true ATE447615T1 (de) 2009-11-15

Family

ID=18952423

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02708684T ATE447615T1 (de) 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein

Country Status (14)

Country Link
US (1) US7252972B2 (de)
EP (1) EP1375664B1 (de)
JP (1) JP4362651B2 (de)
KR (1) KR100602807B1 (de)
CN (1) CN1250722C (de)
AT (1) ATE447615T1 (de)
AU (1) AU2002242986A1 (de)
BR (1) BR0208136B1 (de)
CA (1) CA2442679C (de)
DE (1) DE60234247D1 (de)
DK (1) DK1375664T3 (de)
ES (1) ES2335750T3 (de)
RU (1) RU2264463C2 (de)
WO (1) WO2002081694A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2000230762A1 (en) 2000-01-21 2001-07-31 Ajinomoto Co. Inc. Process for producing l-lysine
RU2316594C2 (ru) * 2003-03-07 2008-02-10 Адзиномото Ко., Инк. Способ продуцирования микробной трансглутаминазы
DE10359660A1 (de) * 2003-12-18 2005-07-28 Basf Ag Psod-Expressionseinheiten
DE10359594A1 (de) * 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
EP1748077B1 (de) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Verfahren zur proteinherstellung
DK1756273T3 (da) * 2004-06-14 2010-02-01 Novozymes As Signalpeptid til frembringelse af et polypeptid
US7527947B2 (en) 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
DE102004035065A1 (de) * 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) * 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
WO2007009082A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
RU2338784C2 (ru) * 2006-03-24 2008-11-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Новая альдолаза, днк, кодирующая альдолазу, клетки, трансформированные днк, способ получения альдолазы и способ получения 4-гидрокси-l-изолейцина (варианты)
EP2082045B1 (de) * 2006-10-24 2015-03-18 Basf Se Verfahren zur reduzierung der genexpression mittels modifizierter codonverwendung
EP2123756B1 (de) * 2007-02-15 2014-06-04 Ajinomoto Co., Inc. Transglutaminase mit darin eingeführter disulfidbindung
ES2480540T3 (es) 2007-03-29 2014-07-28 Ajinomoto Co., Inc. Preparación enzimática para la adherencia y procedimiento de producción de un producto alimenticio moldeado por adherencia
JPWO2009066785A1 (ja) 2007-11-19 2011-04-07 味の素株式会社 繊維加工物及びその製造法
JP5445453B2 (ja) * 2008-07-09 2014-03-19 味の素株式会社 アミノヒドロキシ安息香酸類の製造方法
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
EP2404999B1 (de) 2009-03-06 2015-04-29 Ajinomoto Co., Inc. Wärmetolerante transglutaminase aus actinomyces
WO2011105344A1 (ja) 2010-02-23 2011-09-01 東レ株式会社 カダベリンの製造方法
JP6064912B2 (ja) * 2011-10-25 2017-01-25 味の素株式会社 タンパク質の分泌生産法
EP2774982B1 (de) 2011-11-02 2017-06-21 Ajinomoto Co., Inc. Verfahren zur sekretion und produktion von proteinen
JP6020581B2 (ja) 2011-11-02 2016-11-02 味の素株式会社 タンパク質の分泌生産法
CA2868469A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
EP2831098A4 (de) 2012-03-26 2016-12-07 Axcella Health Inc Geladene nährstoffproteine und verfahren
HK1206764A1 (en) 2012-03-26 2016-01-15 Axcella Health Inc. Nutritive proteins and methods
SG11201405841RA (en) 2012-03-26 2014-10-30 Pronutria Inc Nutritive fragments, proteins and methods
SG11201602012RA (en) 2013-09-25 2016-04-28 Pronutria Inc Compositions and formulations for maintaining and increasing muscle mass, strength, and performance and methods of production and use thereof
DK3287523T3 (da) 2015-04-24 2022-07-25 Ajinomoto Kk Fremgangsmåde til sekretorisk fremstilling af protein
JP6729600B2 (ja) 2015-11-12 2020-07-22 味の素株式会社 Ｎε−アシル−Ｌ−リジンの製造方法
JP7020403B2 (ja) 2016-05-02 2022-02-16 味の素株式会社 アジド基含有Ｆｃタンパク質
DK3533875T5 (da) 2016-09-01 2024-09-02 Ningxia Eppen Biotech Co Ltd Corynebacterium til fremstilling af l-lysin ved fermentering
JP7021639B2 (ja) 2016-10-21 2022-02-17 味の素株式会社 タンパク質の分泌生産法
CN109937258B (zh) 2016-10-21 2023-06-23 味之素株式会社 蛋白质的分泌产生方法
EP3572522A4 (de) 2017-01-19 2020-10-28 Ajinomoto Co., Inc. Verfahren zur herstellung einer heparosanverbindung mit isomerisiertem hexulonsäurerest
EP3415622A1 (de) 2017-06-14 2018-12-19 Evonik Degussa GmbH Verfahren zur herstellung von feinchemikalien mittels eines corynebakteriums, welches modifizierte alpha-1,6-glucosidasen sezerniert
JP7200948B2 (ja) 2017-09-05 2023-01-10 味の素株式会社 ２－ｏ－硫酸化酵素変異体、および３－ｏ－硫酸化酵素変異体、ならびにそれらを用いる方法
JP7439749B2 (ja) 2018-04-20 2024-02-28 味の素株式会社 タンパク質の分泌生産法
JP7375767B2 (ja) 2018-10-25 2023-11-08 味の素株式会社 タンパク質の分泌生産法
JPWO2020090979A1 (ja) 2018-10-31 2021-09-24 味の素株式会社 抗体に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩
CN115243561A (zh) 2020-03-04 2022-10-25 味之素株式会社 突变型转谷氨酰胺酶
CN116456832A (zh) 2020-09-29 2023-07-18 味之素株式会社 变异型谷氨酰胺转氨酶
AU2022306337A1 (en) 2021-07-07 2024-02-01 Ajinomoto Co., Inc. Method for secretory production of unnatural-amino-acid-containing protein
WO2023200008A1 (ja) 2022-04-15 2023-10-19 味の素株式会社 培養肉の製造方法
KR20250019100A (ko) 2022-06-02 2025-02-07 아지노모토 가부시키가이샤 친화성 물질, 화합물, 항체 및 그것들의 염
EP4667580A1 (de) 2023-02-16 2025-12-24 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung von proteinen

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0158940B1 (de) 1984-04-04 1991-12-11 Ajinomoto Co., Inc. Zusammengesetztes transduzierbares Plasmid
JPH07112431B2 (ja) * 1985-09-06 1995-12-06 味の素株式会社 遺伝子発現調節法
RU2091490C1 (ru) * 1985-10-25 1997-09-27 Зимодженетикс, Инк. Способ получения гетерологичного полипептида в эукариотических микроорганизмов
US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
WO1993003158A1 (fr) 1991-07-30 1993-02-18 Orsan Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries
ATE165606T1 (de) 1993-01-13 1998-05-15 Ajinomoto Kk Zelloberflächenprotein aus brevibakterium lactofermentum
JP3711658B2 (ja) 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
EP0897003B1 (de) 1997-07-05 2004-05-12 Societe Des Produits Nestle S.A. Verstärkte Expression von proteolytischen Enzymen in Koji Pilz
JPH11169182A (ja) * 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
ES2372806T3 (es) * 1999-09-30 2012-01-26 Ajinomoto Co., Inc. Proceso para la producción de transglutaminasa.

Also Published As

Publication number Publication date
RU2003128977A (ru) 2005-02-27
DK1375664T3 (da) 2010-02-08
DE60234247D1 (de) 2009-12-17
BR0208136A (pt) 2004-03-02
KR20030087042A (ko) 2003-11-12
CN1250722C (zh) 2006-04-12
AU2002242986A1 (en) 2002-10-21
BR0208136B1 (pt) 2014-10-21
ES2335750T3 (es) 2010-04-05
JP4362651B2 (ja) 2009-11-11
JPWO2002081694A1 (ja) 2004-07-29
WO2002081694A1 (fr) 2002-10-17
US7252972B2 (en) 2007-08-07
RU2264463C2 (ru) 2005-11-20
WO2002081694A8 (fr) 2002-11-14
CN1500145A (zh) 2004-05-26
KR100602807B1 (ko) 2006-07-20
CA2442679C (en) 2010-06-01
EP1375664A4 (de) 2005-08-17
CA2442679A1 (en) 2002-10-17
EP1375664B1 (de) 2009-11-04
EP1375664A1 (de) 2004-01-02
US20040126847A1 (en) 2004-07-01

Similar Documents

Publication Publication Date Title
DE60234247D1 (de) Verfahren zur sekretion und produktion von protein
ATE532870T1 (de) Verfahren zur herstellung von transglutaminase
MXPA04004874A (es) Procedimiento para producir l-aminoacido utilizando escherichia.
NO20013801D0 (no) Ny karbonyl reduktase, gen derav og metode for anvendelse av det samme
DE60033553D1 (de) Verfahren zur herstellung von tripeptiden
EE05008B1 (et) Meetod kiraalsete karboksüülhapete saamiseks nitriilidest nitrilaasi v?i mikroorganismide abil, missisaldavad ühte geeni nitrilaasi jaoks
ATE290591T1 (de) Für adseverin kodierendes gen
ATE461997T1 (de) Neuartige glukose-6-phosphat dehydrogenase
ATE329927T1 (de) Rekombinante coryneformbakterie die glyceraldehyde-3-phosphate dehydrogenase -2 überexprimieren , und verfahren zur herstellung von l-lysine
PL348447A1 (en) L-glutamic acid production bacterium and method of obtaining l-glutamic acid
ATE124724T1 (de) Penicillin-g-acylase, dafür kodierendes gen und verfahren zur herstellung von diesem enzym.
ATE171728T1 (de) Trypsintyp-enzyme, dafür kodierende nukleinsäuresequenz und verfahren zur herstellung des enzymes
ATE480624T1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
DE60139207D1 (de) NUKLEOTIDSEQUENZEN, DIE FÜR DAS metY-GEN KODIEREN
DE60220797D1 (de) Nucleinsäuresequenzen kodierend für enantioselektive amidasen
EP1712627A4 (de) Polypeptid mit amidaseaktivität und dessen gen
WO2002002775A3 (de) Calpain-protease 12
WO2002055711A3 (de) Verfahren zur fermentativen herstellung von pantothensäure
ATE380863T1 (de) Neue, aus bacillus licheniformis stammende aminopeptidase, die aminopeptidase codierendes gen, das gen enthaltender expressionsvektor, transformant sowie verfahren zur herstellung davon
ATE269903T1 (de) Verbessertes, induktionsfreies verfahren zur gentechnischen herstellung von glutarylamidase

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties