ATE460488T1 - Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen - Google Patents

Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Info

Publication number
ATE460488T1
ATE460488T1 AT03724363T AT03724363T ATE460488T1 AT E460488 T1 ATE460488 T1 AT E460488T1 AT 03724363 T AT03724363 T AT 03724363T AT 03724363 T AT03724363 T AT 03724363T AT E460488 T1 ATE460488 T1 AT E460488T1
Authority
AT
Austria
Prior art keywords
dna sequence
plasmids
region
dna
variants
Prior art date
Application number
AT03724363T
Other languages
English (en)
Inventor
Howard Brody
Suzanne Otani
Joel Cherry
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE460488T1 publication Critical patent/ATE460488T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724363T 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen ATE460488T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37468802P 2002-04-22 2002-04-22
PCT/US2003/013577 WO2003089648A1 (en) 2002-04-22 2003-04-22 Methods for preparing variants of a dna sequence in filamentous fungi

Publications (1)

Publication Number Publication Date
ATE460488T1 true ATE460488T1 (de) 2010-03-15

Family

ID=29251220

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724363T ATE460488T1 (de) 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Country Status (7)

Country Link
US (1) US20030199038A1 (de)
EP (1) EP1499733B1 (de)
AT (1) ATE460488T1 (de)
AU (1) AU2003231228A1 (de)
DE (1) DE60331644D1 (de)
DK (1) DK1499733T3 (de)
WO (1) WO2003089648A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939304B2 (en) * 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
AU2003282724B2 (en) * 2002-10-02 2010-03-04 Catalyst Biosciences, Inc. Methods of generating and screening for proteases with altered specificity
US20060024289A1 (en) * 2002-10-02 2006-02-02 Ruggles Sandra W Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases
JP5629423B2 (ja) * 2004-04-12 2014-11-19 カタリスト バイオサイエンシーズ， インコーポレイテッド 野生型及び変異型ｍｔ−ｓｐ１による、ｖｅｇｆ及びｖｅｇｆ受容体の切断
EP1777292A1 (de) * 2005-10-19 2007-04-25 Signalomics GmbH Verfahren zum Erzeugen von genetischer Diversität in vivo
US10337019B2 (en) * 2016-01-25 2019-07-02 Intact Genomics, Inc. Fungal artificial chromosomes, compositions, methods and uses therefor

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518584A (en) 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0208491B1 (de) 1985-07-03 1993-08-25 Genencor International, Inc. Hybride Polypeptide und Verfahren zu deren Herstellung
US4894331A (en) 1985-09-27 1990-01-16 Amgen Inc. Partial marker cassette mutagenesis of xylose isomerase
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB9114734D0 (en) 1991-07-09 1991-08-28 Univ London Process for modifying proteins
FI964808A0 (fi) 1994-06-03 1996-12-02 Novo Nordisk Biotech Inc Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
DE69620766T2 (de) * 1995-08-11 2004-11-18 Novozymes A/S Verfahren zur herstellung von polypeptidabkömmlingen
CN1231588C (zh) 1996-01-19 2005-12-14 诺沃奇梅兹生物技术有限公司 丝状真菌的形态突变体
JP3462218B2 (ja) 1996-06-10 2003-11-05 ノボザイムス バイオテック，インコーポレイティド 糸状菌におけるヘムタンパク質生産を増加せしめるための方法
AU719536B2 (en) 1996-06-28 2000-05-11 Novozymes A/S A recombinant enzyme with dextranase activitiy
JP4436934B2 (ja) 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
AU4213999A (en) 1998-05-27 1999-12-13 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
AU3900700A (en) * 1999-03-17 2000-10-04 Paradigm Genetics, Inc. Methods and materials for the rapid and high volume production of a gene knock-out library in an organism

Also Published As

Publication number Publication date
AU2003231228A1 (en) 2003-11-03
EP1499733A4 (de) 2005-09-14
DK1499733T3 (da) 2010-07-12
EP1499733B1 (de) 2010-03-10
EP1499733A1 (de) 2005-01-26
US20030199038A1 (en) 2003-10-23
WO2003089648A1 (en) 2003-10-30
DE60331644D1 (de) 2010-04-22

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