ATE460488T1 - Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen - Google Patents

Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Info

Publication number
ATE460488T1
ATE460488T1 AT03724363T AT03724363T ATE460488T1 AT E460488 T1 ATE460488 T1 AT E460488T1 AT 03724363 T AT03724363 T AT 03724363T AT 03724363 T AT03724363 T AT 03724363T AT E460488 T1 ATE460488 T1 AT E460488T1
Authority
AT
Austria
Prior art keywords
dna sequence
plasmids
region
dna
variants
Prior art date
Application number
AT03724363T
Other languages
English (en)
Inventor
Howard Brody
Suzanne Otani
Joel Cherry
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE460488T1 publication Critical patent/ATE460488T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724363T 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen ATE460488T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37468802P 2002-04-22 2002-04-22
PCT/US2003/013577 WO2003089648A1 (en) 2002-04-22 2003-04-22 Methods for preparing variants of a dna sequence in filamentous fungi

Publications (1)

Publication Number Publication Date
ATE460488T1 true ATE460488T1 (de) 2010-03-15

Family

ID=29251220

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724363T ATE460488T1 (de) 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Country Status (7)

Country Link
US (1) US20030199038A1 (de)
EP (1) EP1499733B1 (de)
AT (1) ATE460488T1 (de)
AU (1) AU2003231228A1 (de)
DE (1) DE60331644D1 (de)
DK (1) DK1499733T3 (de)
WO (1) WO2003089648A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060024289A1 (en) * 2002-10-02 2006-02-02 Ruggles Sandra W Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases
WO2004031733A2 (en) * 2002-10-02 2004-04-15 Catalyst Biosciences Methods of generating and screenign for porteases with altered specificity
US7939304B2 (en) * 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
WO2005110453A2 (en) * 2004-04-12 2005-11-24 Catalyst Biosciences, Inc. Cleavage of vegf and vegf receptor by wildtype and mutant mt-sp1
EP1777292A1 (de) * 2005-10-19 2007-04-25 Signalomics GmbH Verfahren zum Erzeugen von genetischer Diversität in vivo
US10337019B2 (en) * 2016-01-25 2019-07-02 Intact Genomics, Inc. Fungal artificial chromosomes, compositions, methods and uses therefor

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518584A (en) 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3688920D1 (de) 1985-07-03 1993-09-30 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
US4894331A (en) * 1985-09-27 1990-01-16 Amgen Inc. Partial marker cassette mutagenesis of xylose isomerase
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB9114734D0 (en) 1991-07-09 1991-08-28 Univ London Process for modifying proteins
CA2191718A1 (en) 1994-06-03 1995-12-14 Randy M. Berka Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
WO1997007205A1 (en) * 1995-08-11 1997-02-27 Novo Nordisk A/S Method for preparing polypeptide variants
AU1750697A (en) 1996-01-19 1997-08-11 Novo Nordisk Biotech, Inc. Morphological mutants of filamentous fungi
CN1267556C (zh) 1996-06-10 2006-08-02 诺沃奇梅兹有限公司 提高丝状真菌中血红素蛋白产量的方法
JP2000514289A (ja) 1996-06-28 2000-10-31 ノボ ノルディスク アクティーゼルスカブ デキストラナーゼ活性を有する組換え酵素
JP4436934B2 (ja) 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
EP1080210A2 (de) 1998-05-27 2001-03-07 Novo Nordisk Biotech, Inc. Methoden zur herstellung eines polypeptides durch modifizierung der kopiezahl des gens
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
JP2003502015A (ja) * 1999-03-17 2003-01-21 パラダイム ジェネティックス、 インコーポレイテッド 生物中における遺伝子ノックアウトライブラリの迅速かつ大量産生のための方法類および素材類

Also Published As

Publication number Publication date
US20030199038A1 (en) 2003-10-23
DE60331644D1 (de) 2010-04-22
EP1499733A4 (de) 2005-09-14
WO2003089648A1 (en) 2003-10-30
EP1499733A1 (de) 2005-01-26
DK1499733T3 (da) 2010-07-12
AU2003231228A1 (en) 2003-11-03
EP1499733B1 (de) 2010-03-10

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