ATE469239T1 - Verfahren zum nachweis lebensfähiger zellen in einer probe unter verwendung eines virus - Google Patents

Verfahren zum nachweis lebensfähiger zellen in einer probe unter verwendung eines virus

Info

Publication number
ATE469239T1
ATE469239T1 AT06709788T AT06709788T ATE469239T1 AT E469239 T1 ATE469239 T1 AT E469239T1 AT 06709788 T AT06709788 T AT 06709788T AT 06709788 T AT06709788 T AT 06709788T AT E469239 T1 ATE469239 T1 AT E469239T1
Authority
AT
Austria
Prior art keywords
virus
sample
viable cells
cells
detecting viable
Prior art date
Application number
AT06709788T
Other languages
English (en)
Inventor
Martin Alan Lee
David James Squirrell
Original Assignee
Enigma Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enigma Diagnostics Ltd filed Critical Enigma Diagnostics Ltd
Application granted granted Critical
Publication of ATE469239T1 publication Critical patent/ATE469239T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT06709788T 2005-02-16 2006-02-16 Verfahren zum nachweis lebensfähiger zellen in einer probe unter verwendung eines virus ATE469239T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0503172.9A GB0503172D0 (en) 2005-02-16 2005-02-16 Detection method
PCT/GB2006/000551 WO2006087559A1 (en) 2005-02-16 2006-02-16 Method for detecting viable cells in a sample by using a virus

Publications (1)

Publication Number Publication Date
ATE469239T1 true ATE469239T1 (de) 2010-06-15

Family

ID=34385553

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06709788T ATE469239T1 (de) 2005-02-16 2006-02-16 Verfahren zum nachweis lebensfähiger zellen in einer probe unter verwendung eines virus

Country Status (12)

Country Link
US (2) US8071337B2 (de)
EP (1) EP1851325B1 (de)
JP (1) JP4903722B2 (de)
KR (1) KR20070112796A (de)
CN (1) CN101160410B (de)
AT (1) ATE469239T1 (de)
AU (1) AU2006215415B2 (de)
CA (1) CA2597835A1 (de)
DE (1) DE602006014505D1 (de)
DK (1) DK1851325T3 (de)
GB (1) GB0503172D0 (de)
WO (1) WO2006087559A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0719425A2 (pt) * 2006-11-29 2014-02-25 Microsens Medtech Ltd Métodos para a captura de uma amostra de microrganismos tendo uma superfície hidrofóbica, e para a detecção de um microorganismo, e, kit de ensaio de microrganismo
AU2008236615B2 (en) * 2007-04-05 2013-10-03 Sequella, Inc. Improved methods and compositions for determining the pathogenic status of infectious agents
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
WO2021186114A1 (fr) * 2020-03-20 2021-09-23 Prodose Procede et dispositif de detection et de suivi de la presence, du developpement et de la propagation d'agents infectieux, notamment des bacteries et des virus
CN112080475B (zh) * 2020-07-30 2022-02-11 扬州大学 一种副溶血性弧菌噬菌体及其在检测副溶血性弧菌大流行株活细胞含量中的应用
CN112063732B (zh) * 2020-09-17 2022-05-31 扬州大学 一种能够特异性识别阪崎肠杆菌存活细胞的快速定量检测方法及其引物

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2944694B2 (ja) 1988-10-04 1999-09-06 ディーエヌエー プラント テクノロジー コーポレイション 検出可能な表現型のファージ形質導入による細菌の検出
US5888725A (en) 1992-09-22 1999-03-30 The Secretary Of State For The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Method for identifying target bacteria
CA2130072C (en) * 1994-08-12 2003-02-18 John W. Cherwonogrodzky Method of detecting a pathogen using a virus
DE69534386T2 (de) * 1995-08-25 2006-04-20 Affymetrix, Inc. (a Delaware Corp.), Santa Clara Freigabe von intrazellulärem material
GB9525661D0 (en) 1995-12-15 1996-02-14 Biotec Diagnostics Limited Method
US5958675A (en) 1997-04-18 1999-09-28 3M Innovative Properties Company Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
WO2002072776A2 (en) 2001-03-09 2002-09-19 The Regents Of The University Of California Cell culture system for synthesis of infectious hepatitis c virus
WO2003035889A2 (en) 2001-07-13 2003-05-01 Investigen, Inc. Compositions and methods for bacteria detection
AU2003284228A1 (en) 2002-10-15 2004-05-04 Dwight L. Anderson Assays to detect or quantify bacterial or viral pathogens and contaminants
EP1613965A2 (de) 2003-04-10 2006-01-11 Kent J. Voorhees Vorrichtung und verfahren zum nachweis mikroskopischer lebender organismen mittels bakteriophagen
GB0525072D0 (en) 2005-12-09 2006-01-18 Enigma Diagnostics Ltd Fluorescence-based detection methods and apparatus

Also Published As

Publication number Publication date
AU2006215415A1 (en) 2006-08-24
CN101160410B (zh) 2016-03-30
JP4903722B2 (ja) 2012-03-28
EP1851325B1 (de) 2010-05-26
EP1851325A1 (de) 2007-11-07
DK1851325T3 (da) 2010-08-30
CN101160410A (zh) 2008-04-09
US8071337B2 (en) 2011-12-06
WO2006087559A1 (en) 2006-08-24
KR20070112796A (ko) 2007-11-27
US20080166700A1 (en) 2008-07-10
JP2008529548A (ja) 2008-08-07
CA2597835A1 (en) 2006-08-24
US8609375B2 (en) 2013-12-17
US20120141976A1 (en) 2012-06-07
DE602006014505D1 (de) 2010-07-08
GB0503172D0 (en) 2005-03-23
AU2006215415B2 (en) 2011-01-06

Similar Documents

Publication Publication Date Title
BRPI0922572A2 (pt) método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
WO2009060847A1 (ja) 核酸増幅用サンプルの調製方法及び調製キット
DK2499236T3 (da) Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand
WO2010020876A3 (en) Cell culture method to form aggregates
BRPI0921966A8 (pt) Célula hospedeira de levedura termotolerante, método de fermentar celulose e cultura
WO2008079469A3 (en) Genetically engineered biological indicator
EP2169048A3 (de) System und Verfahren zur Kultivierung von Zellen
WO2007140015A3 (en) Biochemical analysis of partitioned cells
WO2010089151A8 (en) Bioreactor for the cultivation of mammalian cells
WO2009022558A1 (ja) 微生物検出法及び微生物検出キット
BR112013020217A2 (pt) método e biorreator para o cultivo de micro-organismos
BR112013000413A2 (pt) processo para detectar e identificar diretamente um microrganismo em uma amostra biológica diluída em um caldo de enriquecimento.
WO2009114185A3 (en) Methods and compositions for translational profiling and molecular phenotyping
MX2010000031A (es) Deteccion mejorada de microorganismos.
NZ701866A (en) Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
BRPI0607540A2 (pt) ensaio para a produção de circovìrus porcino
EP2246414A4 (de) Zellkulturverfahren und screeningverfahren
ATE461443T1 (de) Verfahren zur bestimmung der viabilität von zellen in zellkulturen
DK1989544T3 (da) T-celle-testmetode
ATE555196T1 (de) Wärmebeständige duplexspezifische nuklease
JP2013535979A5 (de)
ATE469239T1 (de) Verfahren zum nachweis lebensfähiger zellen in einer probe unter verwendung eines virus
EP2644706A3 (de) Verfahren zur Beurteilung von kultivierten Zellen und Verfahren zum Screenen von Biomarkern
ATE499598T1 (de) Markierungsfreier einzelzelltest
BRPI1008762B8 (pt) métodos para seleção de células de mamífero expressando uma proteína heteróloga, para produzir um produto de interesse e uso de um meio de cultura seletivo

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties