DK1625219T3 - Fremgangsmåder til fremstilling af secernerede polypeptider - Google Patents

Fremgangsmåder til fremstilling af secernerede polypeptider

Info

Publication number
DK1625219T3
DK1625219T3 DK04751203.3T DK04751203T DK1625219T3 DK 1625219 T3 DK1625219 T3 DK 1625219T3 DK 04751203 T DK04751203 T DK 04751203T DK 1625219 T3 DK1625219 T3 DK 1625219T3
Authority
DK
Denmark
Prior art keywords
nucleotide sequence
polypeptide
signal peptide
methods
fungal host
Prior art date
Application number
DK04751203.3T
Other languages
English (en)
Inventor
Suchindra Maiyuran
Ana Fidantsef
Howard Brody
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK1625219T3 publication Critical patent/DK1625219T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK04751203.3T 2003-05-02 2004-04-30 Fremgangsmåder til fremstilling af secernerede polypeptider DK1625219T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46776603P 2003-05-02 2003-05-02
PCT/US2004/013711 WO2004111228A1 (en) 2003-05-02 2004-04-30 Methods for producing secreted polypeptides

Publications (1)

Publication Number Publication Date
DK1625219T3 true DK1625219T3 (da) 2010-11-01

Family

ID=33551405

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04751203.3T DK1625219T3 (da) 2003-05-02 2004-04-30 Fremgangsmåder til fremstilling af secernerede polypeptider

Country Status (8)

Country Link
US (4) US7393664B2 (da)
EP (1) EP1625219B1 (da)
CN (1) CN1836038B (da)
AT (1) ATE478140T1 (da)
DE (1) DE602004028724D1 (da)
DK (1) DK1625219T3 (da)
ES (1) ES2350903T3 (da)
WO (1) WO2004111228A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2437198T3 (es) * 2003-10-28 2014-01-09 Novozymes Inc. Polipéptidos con actividad de beta-glucosidasa y polinucleótidos aislados que codifican los polipéptidos
DK1735454T3 (da) 2004-03-25 2017-08-28 Novozymes Inc Fremgangsmåder til nedbrydning eller omdannelse af plantecellevægspolysaccharider
WO2006125068A2 (en) * 2005-05-19 2006-11-23 Novozymes North America, Inc Production of enzymes
ATE503018T1 (de) * 2006-06-26 2011-04-15 Dsm Ip Assets Bv Transektion filamentöser pilze mit hohem durchsatz
DK2046819T3 (da) 2006-07-21 2015-06-22 Novozymes Inc Fremgangsmåder til forøgelse af secernering af polypeptider med biologisk aktivitet
BRPI0817474B1 (pt) * 2007-09-28 2018-04-24 Novozymes A/S Célula hospedeira microbiana transgênica, construção de ácido nucleico, vetor de expressão, composição detergente, e, métodos para produzir uma proteína, para degradar ou converter um material celulósico, e para produzir um produto de fermentação
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EP2397491A1 (en) 2010-06-21 2011-12-21 Technische Universität Wien LeaA from Trichoderma reesei
KR20130132789A (ko) * 2010-11-05 2013-12-05 아사히 가라스 가부시키가이샤 스키조사카로미세스속 효모의 형질 전환체 및 그 제조 방법
BR112014004186B1 (pt) 2011-08-24 2020-12-15 Novozymes, Inc. Composição enzimática, célula hospedeira de fungo filamentoso recombinante, método para produzir uma composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação, e, para fermentar um material celulósico
CN103184159B (zh) * 2011-12-27 2016-09-21 丰益(上海)生物技术研发中心有限公司 紧密枝顶孢霉菌株及其特异性脂肪酶
EP2708553A1 (en) 2012-09-18 2014-03-19 Technische Universität Wien Modified fungal cell
CA2905033C (en) 2013-03-15 2023-06-27 Lallemand Hungary Liquidity Management Llc Expression of beta-glucosidases for hydrolysis of lignocellulose and associated oligomers
US20170166939A1 (en) 2013-11-26 2017-06-15 Novozymes A/S Enzyme Compositions and Uses Thereof
EP3152315B1 (en) 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
WO2016145084A1 (en) 2015-03-09 2016-09-15 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN104975039B (zh) * 2015-06-24 2018-05-11 方诩 一种重组质粒及其在降解纤维素原料中的应用
CN108350470B (zh) 2015-10-30 2022-01-11 三得利控股株式会社 使用aobgl3同源体的甜菊醇糖苷及甜菊醇的制造方法
EP3369824A4 (en) * 2015-10-30 2019-04-03 Suntory Holdings Limited PROCESS FOR THE PREPARATION OF STEVIOL AND STEVIOL GLYCOSIDE USING AN AOBGL1 HOMOLOGOUS
US20210284991A1 (en) 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
JP6839424B2 (ja) * 2016-12-27 2021-03-10 国立大学法人 鹿児島大学 真菌におけるタンパク質の選択的分泌技術
CN113015782A (zh) * 2018-11-19 2021-06-22 巴斯夫欧洲公司 用于酵母的前导序列
CN112048468B (zh) * 2019-09-03 2021-04-06 南京市妇幼保健院 一种卵母细胞体外成熟培养试剂
CN111378674B (zh) * 2020-03-25 2021-09-10 中国科学院天津工业生物技术研究所 异宗毁丝霉糖化酶MhglaA及其编码基因和在葡萄糖生产中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220721A (en) * 1979-04-27 1980-09-02 University Of Arkansas Foundation Method for enzyme reutilization
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
CA2082279C (en) 1990-05-09 2007-09-04 Grethe Rasmussen Cellulase preparation comprising an endoglucanase enzyme
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US6159720A (en) * 1996-07-24 2000-12-12 Meiji Seika Kaisha, Ltd. Enzyme endoglucanase and cellulase preparations containing the same
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Also Published As

Publication number Publication date
WO2004111228A1 (en) 2004-12-23
US7393664B2 (en) 2008-07-01
EP1625219A1 (en) 2006-02-15
US20120122191A1 (en) 2012-05-17
ATE478140T1 (de) 2010-09-15
EP1625219A4 (en) 2007-09-05
DE602004028724D1 (de) 2010-09-30
US20080268526A1 (en) 2008-10-30
CN1836038B (zh) 2011-04-06
EP1625219B1 (en) 2010-08-18
CN1836038A (zh) 2006-09-20
US8497115B2 (en) 2013-07-30
US20140011262A1 (en) 2014-01-09
ES2350903T3 (es) 2011-01-28
US20040248258A1 (en) 2004-12-09

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