ATE517919T1 - Spaltung von insulinvorstufen durch eine trypsinvariante - Google Patents
Spaltung von insulinvorstufen durch eine trypsinvarianteInfo
- Publication number
- ATE517919T1 ATE517919T1 AT06791678T AT06791678T ATE517919T1 AT E517919 T1 ATE517919 T1 AT E517919T1 AT 06791678 T AT06791678 T AT 06791678T AT 06791678 T AT06791678 T AT 06791678T AT E517919 T1 ATE517919 T1 AT E517919T1
- Authority
- AT
- Austria
- Prior art keywords
- classification
- insulin precursors
- trypsin variant
- trypsin
- variant
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title 2
- 102000004877 Insulin Human genes 0.000 title 1
- 108090001061 Insulin Proteins 0.000 title 1
- 102000004142 Trypsin Human genes 0.000 title 1
- 108090000631 Trypsin Proteins 0.000 title 1
- 229940125396 insulin Drugs 0.000 title 1
- 239000002243 precursor Substances 0.000 title 1
- 239000012588 trypsin Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Diabetes (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05077086 | 2005-09-14 | ||
| PCT/EP2006/008380 WO2007031187A1 (en) | 2005-09-14 | 2006-08-26 | Cleavage of precursors of insulins by a variant of trypsin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE517919T1 true ATE517919T1 (de) | 2011-08-15 |
Family
ID=36190640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06791678T ATE517919T1 (de) | 2005-09-14 | 2006-08-26 | Spaltung von insulinvorstufen durch eine trypsinvariante |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US7981635B2 (de) |
| EP (1) | EP1926749B1 (de) |
| JP (1) | JP5027812B2 (de) |
| KR (1) | KR101304958B1 (de) |
| CN (1) | CN101253196B (de) |
| AR (1) | AR055428A1 (de) |
| AT (1) | ATE517919T1 (de) |
| AU (1) | AU2006291780B2 (de) |
| BR (1) | BRPI0616054B8 (de) |
| CA (1) | CA2621344C (de) |
| CY (1) | CY1112667T1 (de) |
| ES (1) | ES2370657T3 (de) |
| IL (1) | IL189887A (de) |
| MX (1) | MX2008003362A (de) |
| MY (1) | MY146082A (de) |
| PL (1) | PL1926749T3 (de) |
| PT (1) | PT1926749E (de) |
| SI (1) | SI1926749T1 (de) |
| WO (1) | WO2007031187A1 (de) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2524423C2 (ru) | 2008-01-09 | 2014-07-27 | Санофи-Авентис Дойчланд Гмбх | Новые производные инсулина с чрезвычайно замедленным профилем время/действие |
| DE102008025007A1 (de) | 2008-05-24 | 2009-11-26 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| DE102008003568A1 (de) | 2008-01-09 | 2009-07-16 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| KR20100111683A (ko) | 2008-01-09 | 2010-10-15 | 사노피-아벤티스 도이칠란트 게엠베하 | 극히 지연된 시간-작용 프로필을 갖는 신규 인슐린 유도체 |
| DE102008003566A1 (de) | 2008-01-09 | 2009-07-16 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| DE102008025008A1 (de) | 2008-05-24 | 2009-11-26 | Sanofi-Aventis Deutschland Gmbh | Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil |
| JP5781308B2 (ja) * | 2008-02-19 | 2015-09-16 | バイオコン・リミテッドＢｉｏｃｏｎ Ｌｉｍｉｔｅｄ | 異種タンパク質を得る方法およびインスリン類似体 |
| PT3228320T (pt) | 2008-10-17 | 2020-03-26 | Sanofi Aventis Deutschland | Combinação de uma insulina e de um agonista do glp-1 |
| PH12011502708B1 (en) | 2009-07-06 | 2017-11-10 | Sanofi Aventis Deutschland | Insulin preparations containing methionine |
| KR101410845B1 (ko) * | 2009-09-10 | 2014-06-23 | 바이오콘 리미티드 | 새로운 프로리파제-보바인 트립시노겐 융합 단백질 |
| JP5832439B2 (ja) | 2009-11-13 | 2015-12-16 | サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | Ｇｌｐ−１アゴニスト、インスリン及びメチオニンを含む薬学的組成物 |
| SG10201500871TA (en) | 2009-11-13 | 2015-04-29 | Sanofi Aventis Deutschland | Pharmaceutical composition comprising a glp-1 agonist and methionine |
| CN103179978A (zh) | 2010-08-30 | 2013-06-26 | 赛诺菲-安万特德国有限公司 | Ave0010用于制造供治疗2型糖尿病用的药物的用途 |
| US9821032B2 (en) | 2011-05-13 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin |
| WO2013015697A1 (en) * | 2011-07-28 | 2013-01-31 | Mabion S.A. | A recombinant protein, a polynucleotide encoding it as well as a method of obtaining insulin or its an analogue |
| JP6367115B2 (ja) | 2011-08-29 | 2018-08-01 | サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | ２型糖尿病患者の血糖コントロールに使用する組合せ医薬 |
| TWI559929B (en) | 2011-09-01 | 2016-12-01 | Sanofi Aventis Deutschland | Pharmaceutical composition for use in the treatment of a neurodegenerative disease |
| EP2831262B1 (de) * | 2012-03-29 | 2018-02-14 | Biocon Limited | Sekretion von funktionellem insulinglargin direkt in das kulturmedium durch intrazelluläre überexpression von kex2p |
| CN102816785B (zh) * | 2012-05-24 | 2016-03-09 | 上海仁会生物制药股份有限公司 | 一种甘精胰岛素及其类似物的制备方法 |
| CN102994600A (zh) * | 2012-12-11 | 2013-03-27 | 鲁南新时代生物技术有限公司 | 甘精胰岛素前体的酶切转化方法 |
| CN107206058A (zh) | 2014-12-12 | 2017-09-26 | 赛诺菲-安万特德国有限公司 | 甘精胰岛素/利西拉来固定比率配制剂 |
| TWI748945B (zh) | 2015-03-13 | 2021-12-11 | 德商賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患治療 |
| TW201705975A (zh) | 2015-03-18 | 2017-02-16 | 賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患之治療 |
| CN108018277A (zh) * | 2016-11-04 | 2018-05-11 | 江苏万邦生化医药股份有限公司 | 重组牛胰蛋白酶突变体及其制备方法 |
| KR20190036956A (ko) | 2017-09-28 | 2019-04-05 | 한미약품 주식회사 | 지속형 단쇄 인슐린 아날로그 및 이의 결합체 |
| WO2019162423A1 (en) | 2018-02-22 | 2019-08-29 | Sanofi-Aventis Deutschland Gmbh | Variants of porcine trypsin |
| EP3650541A1 (de) * | 2018-11-08 | 2020-05-13 | BioPharma Translationsinstitut Dessau Forschungs GmbH | Trypsin-mutante zur c- und n-terminalen modifizierung von polypeptiden |
| KR20200080747A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법 |
| KR20220112817A (ko) | 2019-12-10 | 2022-08-11 | 사노피 | 설폰아미드 및 폴리펩티드의 콘주게이트를 형성하는 방법 |
| CN113773391B (zh) * | 2020-06-09 | 2023-10-20 | 宁波鲲鹏生物科技有限公司 | 一种门冬胰岛素的制备方法 |
| CN119552847B (zh) * | 2025-01-21 | 2025-04-11 | 四川大学 | 链霉菌胰蛋白酶自剪切位点突变体及其应用 |
| CN121801875A (zh) * | 2026-03-12 | 2026-04-07 | 天津科技大学 | 一种免肠激酶激活的胰蛋白酶的重组酵母表达系统及其构建方法和应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3886043A (en) * | 1972-08-24 | 1975-05-27 | Choay Sa | Process for preparing pure porcine beta-trypsin |
| NZ199391A (en) | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| NZ199722A (en) | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
| DE3382547D1 (de) | 1983-01-12 | 1992-05-27 | Chiron Corp | Sekretorische expression in eukaryoten. |
| US4870008A (en) | 1983-08-12 | 1989-09-26 | Chiron Corporation | Secretory expression in eukaryotes |
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4885242A (en) | 1984-10-30 | 1989-12-05 | Phillips Petroleum Company | Genes from pichia histidine pathway and uses thereof |
| US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
| US4837148A (en) | 1984-10-30 | 1989-06-06 | Phillips Petroleum Company | Autonomous replication sequences for yeast strains of the genus pichia |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| PH25772A (en) | 1985-08-30 | 1991-10-18 | Novo Industri As | Insulin analogues, process for their preparation |
| US4882279A (en) | 1985-10-25 | 1989-11-21 | Phillips Petroleum Company | Site selective genomic modification of yeast of the genus pichia |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US5032516A (en) | 1985-10-25 | 1991-07-16 | Phillips Petroleum Company | Pichia pastoris alcohol oxidase II regulatory region |
| US5166329A (en) | 1985-10-25 | 1992-11-24 | Phillips Petroleum Company | DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia |
| US5135868A (en) | 1985-10-25 | 1992-08-04 | Phillips Petroleum Company | Cultures of yeast of the genus Pichia altered by site selective genomic modification |
| US4895800A (en) | 1985-11-26 | 1990-01-23 | Phillips Petroleum Company | Yeast production of hepatitis B surface antigen |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4857467A (en) | 1986-07-23 | 1989-08-15 | Phillips Petroleum Company | Carbon and energy source markers for transformation of strains of the genes Pichia |
| US5002876A (en) | 1986-09-22 | 1991-03-26 | Phillips Petroleum Company | Yeast production of human tumor necrosis factor |
| US4683293A (en) | 1986-10-20 | 1987-07-28 | Phillips Petroleum Company | Purification of pichia produced lipophilic proteins |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| US5004688A (en) | 1988-04-15 | 1991-04-02 | Phillips Petroleum Company | Purification of hepatitis proteins |
| DK257988D0 (da) | 1988-05-11 | 1988-05-11 | Novo Industri As | Nye peptider |
| HUT56857A (en) | 1988-12-23 | 1991-10-28 | Novo Nordisk As | Human insulin analogues |
| IL93282A (en) | 1989-02-09 | 1995-08-31 | Lilly Co Eli | Insulin analogues |
| US5122465A (en) | 1989-06-12 | 1992-06-16 | Phillips Petroleum Company | Strains of pichia pastoris created by interlocus recombination |
| CA2090969C (en) | 1990-09-04 | 2001-08-21 | Russell Arthur Brierley | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| DE19821866A1 (de) * | 1998-05-15 | 1999-11-18 | Hoechst Marion Roussel De Gmbh | Verfahren zur Katalyse von komplexen Reaktionen großer Moleküle mittels an einem polymeren Träger gebundener Enzyme |
| WO2000056903A2 (en) | 1999-03-22 | 2000-09-28 | Zymogenetics, Inc. | IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) |
| DK1399568T3 (da) * | 2001-02-01 | 2006-03-27 | Hoffmann La Roche | Fremgangsmåde til fremstilling af rekombinant trypsin |
| US7364892B2 (en) * | 2002-09-24 | 2008-04-29 | Novozymes, Inc. | Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same |
-
2006
- 2006-08-26 AT AT06791678T patent/ATE517919T1/de active
- 2006-08-26 JP JP2008530363A patent/JP5027812B2/ja active Active
- 2006-08-26 MX MX2008003362A patent/MX2008003362A/es active IP Right Grant
- 2006-08-26 EP EP06791678A patent/EP1926749B1/de active Active
- 2006-08-26 WO PCT/EP2006/008380 patent/WO2007031187A1/en not_active Ceased
- 2006-08-26 PT PT06791678T patent/PT1926749E/pt unknown
- 2006-08-26 PL PL06791678T patent/PL1926749T3/pl unknown
- 2006-08-26 MY MYPI20080235A patent/MY146082A/en unknown
- 2006-08-26 SI SI200631146T patent/SI1926749T1/sl unknown
- 2006-08-26 KR KR1020087006393A patent/KR101304958B1/ko active Active
- 2006-08-26 CN CN200680031396XA patent/CN101253196B/zh active Active
- 2006-08-26 BR BRPI0616054A patent/BRPI0616054B8/pt active IP Right Grant
- 2006-08-26 AU AU2006291780A patent/AU2006291780B2/en active Active
- 2006-08-26 CA CA2621344A patent/CA2621344C/en active Active
- 2006-08-26 US US12/063,313 patent/US7981635B2/en active Active
- 2006-08-26 ES ES06791678T patent/ES2370657T3/es active Active
- 2006-09-12 AR ARP060103963A patent/AR055428A1/es active IP Right Grant
-
2008
- 2008-03-02 IL IL189887A patent/IL189887A/en active IP Right Grant
-
2011
- 2011-10-27 CY CY20111101023T patent/CY1112667T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007031187A8 (en) | 2007-08-16 |
| CA2621344A1 (en) | 2007-03-22 |
| BRPI0616054B8 (pt) | 2021-05-25 |
| CY1112667T1 (el) | 2016-02-10 |
| AU2006291780A1 (en) | 2007-03-22 |
| MY146082A (en) | 2012-06-29 |
| EP1926749A1 (de) | 2008-06-04 |
| KR20080055843A (ko) | 2008-06-19 |
| ES2370657T3 (es) | 2011-12-21 |
| SI1926749T1 (sl) | 2011-11-30 |
| US7981635B2 (en) | 2011-07-19 |
| JP5027812B2 (ja) | 2012-09-19 |
| EP1926749B1 (de) | 2011-07-27 |
| WO2007031187A1 (en) | 2007-03-22 |
| IL189887A (en) | 2012-02-29 |
| AR055428A1 (es) | 2007-08-22 |
| CN101253196A (zh) | 2008-08-27 |
| PT1926749E (pt) | 2011-09-29 |
| MX2008003362A (es) | 2008-03-25 |
| KR101304958B1 (ko) | 2013-09-17 |
| US20100196953A1 (en) | 2010-08-05 |
| BRPI0616054B1 (pt) | 2019-08-06 |
| BRPI0616054A2 (pt) | 2011-06-07 |
| IL189887A0 (en) | 2008-11-03 |
| JP2009507500A (ja) | 2009-02-26 |
| PL1926749T3 (pl) | 2011-12-30 |
| CA2621344C (en) | 2014-12-16 |
| CN101253196B (zh) | 2012-09-05 |
| HK1124341A1 (en) | 2009-07-10 |
| AU2006291780B2 (en) | 2011-12-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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