BRPI0616054B8 - processo para a preparação de insulina, um análogo de insulina ou um derivado de insulina, tripsina porcina, método para sua produção, dna e vetor - Google Patents

processo para a preparação de insulina, um análogo de insulina ou um derivado de insulina, tripsina porcina, método para sua produção, dna e vetor

Info

Publication number
BRPI0616054B8
BRPI0616054B8 BRPI0616054A BRPI0616054A BRPI0616054B8 BR PI0616054 B8 BRPI0616054 B8 BR PI0616054B8 BR PI0616054 A BRPI0616054 A BR PI0616054A BR PI0616054 A BRPI0616054 A BR PI0616054A BR PI0616054 B8 BRPI0616054 B8 BR PI0616054B8
Authority
BR
Brazil
Prior art keywords
insulin
production
dna
vector
preparation
Prior art date
Application number
BRPI0616054A
Other languages
English (en)
Inventor
Hoh Christoph
Kopetzki Erhard
Geipel Frank
Zocher Frank
Mueller Rainer
Glaser Stephan
Original Assignee
Sanofi Aventis Deutschland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Aventis Deutschland filed Critical Sanofi Aventis Deutschland
Publication of BRPI0616054A2 publication Critical patent/BRPI0616054A2/pt
Publication of BRPI0616054B1 publication Critical patent/BRPI0616054B1/pt
Publication of BRPI0616054B8 publication Critical patent/BRPI0616054B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

clivagem de precursores de insulunas por uma variante de tripsina. a presente invenção refere-se à produção de uma variante de tripsina recombinante com especificidade de substrato aumentada para arginina versus usina, em organismos hospedeiros não-animais. além disso, a presente invenção refere-se a uma variante de tripsina recombinante e sua produção. também proporcionados estão o uso de variantes de tripsina pancreática porcina recombinante para clivagem de precursores de insulinas, e kits contendo a variante de tripsina.
BRPI0616054A 2005-09-14 2006-08-26 processo para a preparação de insulina, um análogo de insulina ou um derivado de insulina, tripsina porcina, método para sua produção, dna e vetor BRPI0616054B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05077086.6 2005-09-14
EP05077086 2005-09-14
PCT/EP2006/008380 WO2007031187A1 (en) 2005-09-14 2006-08-26 Cleavage of precursors of insulins by a variant of trypsin

Publications (3)

Publication Number Publication Date
BRPI0616054A2 BRPI0616054A2 (pt) 2011-06-07
BRPI0616054B1 BRPI0616054B1 (pt) 2019-08-06
BRPI0616054B8 true BRPI0616054B8 (pt) 2021-05-25

Family

ID=36190640

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0616054A BRPI0616054B8 (pt) 2005-09-14 2006-08-26 processo para a preparação de insulina, um análogo de insulina ou um derivado de insulina, tripsina porcina, método para sua produção, dna e vetor

Country Status (19)

Country Link
US (1) US7981635B2 (pt)
EP (1) EP1926749B1 (pt)
JP (1) JP5027812B2 (pt)
KR (1) KR101304958B1 (pt)
CN (1) CN101253196B (pt)
AR (1) AR055428A1 (pt)
AT (1) ATE517919T1 (pt)
AU (1) AU2006291780B2 (pt)
BR (1) BRPI0616054B8 (pt)
CA (1) CA2621344C (pt)
CY (1) CY1112667T1 (pt)
ES (1) ES2370657T3 (pt)
IL (1) IL189887A (pt)
MX (1) MX2008003362A (pt)
MY (1) MY146082A (pt)
PL (1) PL1926749T3 (pt)
PT (1) PT1926749E (pt)
SI (1) SI1926749T1 (pt)
WO (1) WO2007031187A1 (pt)

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DE102008025007A1 (de) 2008-05-24 2009-11-26 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008003568A1 (de) 2008-01-09 2009-07-16 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
KR20100111683A (ko) 2008-01-09 2010-10-15 사노피-아벤티스 도이칠란트 게엠베하 극히 지연된 시간-작용 프로필을 갖는 신규 인슐린 유도체
DE102008003566A1 (de) 2008-01-09 2009-07-16 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008025008A1 (de) 2008-05-24 2009-11-26 Sanofi-Aventis Deutschland Gmbh Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
JP5781308B2 (ja) * 2008-02-19 2015-09-16 バイオコン・リミテッドＢｉｏｃｏｎ Ｌｉｍｉｔｅｄ 異種タンパク質を得る方法およびインスリン類似体
PT3228320T (pt) 2008-10-17 2020-03-26 Sanofi Aventis Deutschland Combinação de uma insulina e de um agonista do glp-1
PH12011502708B1 (en) 2009-07-06 2017-11-10 Sanofi Aventis Deutschland Insulin preparations containing methionine
KR101410845B1 (ko) * 2009-09-10 2014-06-23 바이오콘 리미티드 새로운 프로리파제-보바인 트립시노겐 융합 단백질
JP5832439B2 (ja) 2009-11-13 2015-12-16 サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Ｇｌｐ−１アゴニスト、インスリン及びメチオニンを含む薬学的組成物
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CN103179978A (zh) 2010-08-30 2013-06-26 赛诺菲-安万特德国有限公司 Ave0010用于制造供治疗2型糖尿病用的药物的用途
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
WO2013015697A1 (en) * 2011-07-28 2013-01-31 Mabion S.A. A recombinant protein, a polynucleotide encoding it as well as a method of obtaining insulin or its an analogue
JP6367115B2 (ja) 2011-08-29 2018-08-01 サノフィ−アベンティス・ドイチュラント・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング ２型糖尿病患者の血糖コントロールに使用する組合せ医薬
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
EP2831262B1 (en) * 2012-03-29 2018-02-14 Biocon Limited Secretion of functional insulin glargine directly into the culture medium through over expression of kex2p intracellularly
CN102816785B (zh) * 2012-05-24 2016-03-09 上海仁会生物制药股份有限公司 一种甘精胰岛素及其类似物的制备方法
CN102994600A (zh) * 2012-12-11 2013-03-27 鲁南新时代生物技术有限公司 甘精胰岛素前体的酶切转化方法
CN107206058A (zh) 2014-12-12 2017-09-26 赛诺菲-安万特德国有限公司 甘精胰岛素/利西拉来固定比率配制剂
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
CN108018277A (zh) * 2016-11-04 2018-05-11 江苏万邦生化医药股份有限公司 重组牛胰蛋白酶突变体及其制备方法
KR20190036956A (ko) 2017-09-28 2019-04-05 한미약품 주식회사 지속형 단쇄 인슐린 아날로그 및 이의 결합체
WO2019162423A1 (en) 2018-02-22 2019-08-29 Sanofi-Aventis Deutschland Gmbh Variants of porcine trypsin
EP3650541A1 (en) * 2018-11-08 2020-05-13 BioPharma Translationsinstitut Dessau Forschungs GmbH Trypsin mutant for c- and n-terminal modification of polypeptides
KR20200080747A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법
KR20220112817A (ko) 2019-12-10 2022-08-11 사노피 설폰아미드 및 폴리펩티드의 콘주게이트를 형성하는 방법
CN113773391B (zh) * 2020-06-09 2023-10-20 宁波鲲鹏生物科技有限公司 一种门冬胰岛素的制备方法
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CN121801875A (zh) * 2026-03-12 2026-04-07 天津科技大学 一种免肠激酶激活的胰蛋白酶的重组酵母表达系统及其构建方法和应用

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Also Published As

Publication number Publication date
WO2007031187A8 (en) 2007-08-16
CA2621344A1 (en) 2007-03-22
CY1112667T1 (el) 2016-02-10
AU2006291780A1 (en) 2007-03-22
MY146082A (en) 2012-06-29
EP1926749A1 (en) 2008-06-04
KR20080055843A (ko) 2008-06-19
ES2370657T3 (es) 2011-12-21
SI1926749T1 (sl) 2011-11-30
US7981635B2 (en) 2011-07-19
JP5027812B2 (ja) 2012-09-19
EP1926749B1 (en) 2011-07-27
WO2007031187A1 (en) 2007-03-22
IL189887A (en) 2012-02-29
AR055428A1 (es) 2007-08-22
ATE517919T1 (de) 2011-08-15
CN101253196A (zh) 2008-08-27
PT1926749E (pt) 2011-09-29
MX2008003362A (es) 2008-03-25
KR101304958B1 (ko) 2013-09-17
US20100196953A1 (en) 2010-08-05
BRPI0616054B1 (pt) 2019-08-06
BRPI0616054A2 (pt) 2011-06-07
IL189887A0 (en) 2008-11-03
JP2009507500A (ja) 2009-02-26
PL1926749T3 (pl) 2011-12-30
CA2621344C (en) 2014-12-16
CN101253196B (zh) 2012-09-05
HK1124341A1 (en) 2009-07-10
AU2006291780B2 (en) 2011-12-08

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
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