ATE524541T1 - Kultivierte epidermale zellschicht, laminierte kultivierte hautschicht und verfahren zu deren herstellung - Google Patents

Kultivierte epidermale zellschicht, laminierte kultivierte hautschicht und verfahren zu deren herstellung

Info

Publication number
ATE524541T1
ATE524541T1 AT01945763T AT01945763T ATE524541T1 AT E524541 T1 ATE524541 T1 AT E524541T1 AT 01945763 T AT01945763 T AT 01945763T AT 01945763 T AT01945763 T AT 01945763T AT E524541 T1 ATE524541 T1 AT E524541T1
Authority
AT
Austria
Prior art keywords
cultured
sheet
temperature
critical solution
solution temperature
Prior art date
Application number
AT01945763T
Other languages
English (en)
Inventor
Teruo Okano
Masayuki Yamato
Mika Utsumi
Ai Kushida
Chie Konno
Akihiko Kikuchi
Original Assignee
Cellseed Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellseed Inc filed Critical Cellseed Inc
Application granted granted Critical
Publication of ATE524541T1 publication Critical patent/ATE524541T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/362—Skin, e.g. dermal papillae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT01945763T 2000-07-21 2001-07-02 Kultivierte epidermale zellschicht, laminierte kultivierte hautschicht und verfahren zu deren herstellung ATE524541T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000221383 2000-07-21
PCT/JP2001/005723 WO2002010349A1 (fr) 2000-07-21 2001-07-02 Cellules epidermiques cultivees en couche mince, peau cultivee de façon stratifiee et procede de production associe

Publications (1)

Publication Number Publication Date
ATE524541T1 true ATE524541T1 (de) 2011-09-15

Family

ID=18715794

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01945763T ATE524541T1 (de) 2000-07-21 2001-07-02 Kultivierte epidermale zellschicht, laminierte kultivierte hautschicht und verfahren zu deren herstellung

Country Status (6)

Country Link
US (1) US8889417B2 (de)
EP (3) EP2264148A1 (de)
JP (1) JP4716479B2 (de)
AT (1) ATE524541T1 (de)
ES (1) ES2369253T3 (de)
WO (1) WO2002010349A1 (de)

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EP2267115B8 (de) * 2003-02-06 2014-07-23 Cellseed Inc. Zellblatt des vorderen Augensegments, dreidimensionale Strukturen und Herstellungsverfahren dafür
US20060240400A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato High-density cell array board, process for producing the same and method of using the same
KR101141985B1 (ko) 2003-02-06 2012-05-17 고지 니시다 각막 상피 형성용 세포 시트, 그들의 제조 방법 및 그들의이용 방법
WO2004073761A1 (ja) * 2003-02-20 2004-09-02 Cellseed Inc. 再生角膜内皮細胞シート、製造方法及びその利用方法
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
ES2569780T3 (es) 2003-06-27 2016-05-12 DePuy Synthes Products, Inc. Células posparto derivadas de tejido placentario y métodos de preparación y uso de las mismas
WO2005047496A1 (ja) * 2003-11-14 2005-05-26 Keio University 細胞培養法、細胞の三次元培養法、三次元組織、人工臓器、及び組織移植方法
EP1748064B1 (de) * 2004-04-25 2019-04-17 CellSeed Inc. Kultivierte Periodontalverbindungzellage, Herstellungsverfahren sowie Verwendung davon
WO2006083394A2 (en) * 2004-12-21 2006-08-10 Ethicon, Inc. Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
WO2006101548A2 (en) * 2004-12-21 2006-09-28 Ethicon, Inc. Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
ES2621847T3 (es) * 2004-12-23 2017-07-05 DePuy Synthes Products, Inc. Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas
WO2006093153A1 (ja) 2005-02-28 2006-09-08 Cellseed Inc. 培養細胞シート、製造方法及びその利用した組織修復方法
EP1857126B1 (de) * 2005-02-28 2018-10-24 CellSeed Inc. Gezüchtete zellplatte, herstellungsverfahren und anwendungsverfahren dafür
US20100239498A1 (en) * 2006-01-12 2010-09-23 Kazuo Ohashi Method of maintaining the function of liver tissue cells over long time
JP2007229249A (ja) * 2006-03-01 2007-09-13 Olympus Corp 細胞シートの供給方法
JP4869736B2 (ja) * 2006-03-01 2012-02-08 オリンパス株式会社 細胞シートの取付方法および取付装置
JP4831543B2 (ja) 2006-06-20 2011-12-07 セイコーインスツル株式会社 細胞剥離方法
GB2442271B (en) * 2006-09-27 2011-06-15 Denis Eon Solomon An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells
US8105380B2 (en) * 2006-10-23 2012-01-31 Stemedica Cell Technologies, Inc. Cellular scaffold
US8709081B2 (en) 2006-10-23 2014-04-29 Stemedica Cell Technologies, Inc. Cellular scaffold
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JP5424538B2 (ja) * 2007-03-19 2014-02-26 株式会社セルシード 移植用成熟誘導化樹状細胞、製造方法及びその利用方法
KR100871652B1 (ko) 2007-07-30 2008-12-02 한양대학교 산학협력단 세포 배양용 3차원 마이크로 캐리어, 이의 제조방법 및이를 이용한 세포의 3차원 부유 배양법
US20120015440A1 (en) * 2008-09-08 2012-01-19 Tokyo University Of Science Educational Foundation Administrative Org. Spheroid composite, spheroid-containing hydrogel and processes for production of same
JP5388092B2 (ja) * 2008-09-08 2014-01-15 学校法人東京理科大学 スフェロイド含有ハイドロゲルおよびその製造方法、ならびにスフェロイド含有ハイドロゲル積層体
JP5388093B2 (ja) * 2008-09-08 2014-01-15 学校法人東京理科大学 スフェロイド複合体およびその製造方法、ならびに多層型スフェロイド複合体
WO2010044417A1 (ja) * 2008-10-14 2010-04-22 株式会社セルシード 温度応答性細胞培養器材、及びその製造方法
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JP5407344B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
JP5407345B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
JP5407343B2 (ja) * 2009-01-13 2014-02-05 大日本印刷株式会社 生体組織の作製方法
US20120142103A1 (en) * 2009-05-18 2012-06-07 Kohji Nishida Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells
DE102010012252A1 (de) * 2010-03-22 2011-09-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Thermoresponsives Substrat mit Mikrogelen, Verfahren zu dessen Herstellung und Kultivierungsverfahren für biologische Zellen
WO2011155565A1 (ja) 2010-06-10 2011-12-15 国立大学法人九州工業大学 可逆的な性質を示す温度応答性シートとそれを用いた細胞シートの製造方法
US9546349B2 (en) * 2011-05-31 2017-01-17 Taipei Medical University Supports for cell culture and cell sheet detachment and methods for cell sheet detachment
TWI452138B (zh) * 2011-06-01 2014-09-11 Univ Taipei Medical 用於細胞培養及片狀細胞層脫著之支撐物及片狀細胞層脫著之方法
PL2574664T3 (pl) 2011-09-30 2015-02-27 Centrum Mat Polimerowych I Weglowych Pan Sposób wytwarzania podłoża z powłoką termoczułą, podłoże z powłoką termoczułą oraz jego zastosowanie
JP5713086B2 (ja) * 2013-11-06 2015-05-07 大日本印刷株式会社 生体組織の作製方法
JP6781973B2 (ja) * 2016-01-29 2020-11-11 学校法人東京女子医科大学 腎臓病進行抑制細胞シート組成物、その製造方法、及び、それを用いた腎臓病進行抑制方法
CN106924817B (zh) * 2017-03-02 2019-12-17 浙江大学 一种超薄载体细胞片及其制备方法
KR102227434B1 (ko) * 2017-12-29 2021-03-16 진메디신 주식회사 유전자 전달용 세포 시트
JP7228672B2 (ja) * 2018-07-31 2023-02-24 ロキット ヘルスケア インク． 多層細胞シートの製造方法、及び、これを用いて製造された多層細胞シート
EP3911344A4 (de) 2019-01-16 2022-10-19 University of Utah Research Foundation Mesenchymale stammzellschichten aus menschlicher nabelschnur und verfahren zu ihrer herstellung

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Also Published As

Publication number Publication date
JP4716479B2 (ja) 2011-07-06
EP1312669B1 (de) 2011-09-14
EP1312669A1 (de) 2003-05-21
US20040028657A1 (en) 2004-02-12
WO2002010349A1 (fr) 2002-02-07
US8889417B2 (en) 2014-11-18
ES2369253T3 (es) 2011-11-28
EP2264147A1 (de) 2010-12-22
EP1312669A4 (de) 2007-09-05
EP2264148A1 (de) 2010-12-22
JPWO2002010349A1 (ja) 2004-02-05

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