ATE528647T1 - Verfahren zur untersuchung von protein-protein- interaktionen - Google Patents
Verfahren zur untersuchung von protein-protein- interaktionenInfo
- Publication number
- ATE528647T1 ATE528647T1 AT07758759T AT07758759T ATE528647T1 AT E528647 T1 ATE528647 T1 AT E528647T1 AT 07758759 T AT07758759 T AT 07758759T AT 07758759 T AT07758759 T AT 07758759T AT E528647 T1 ATE528647 T1 AT E528647T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- studying
- activator
- proteins
- interaction
- Prior art date
Links
- 230000003993 interaction Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 150000001875 compounds Chemical class 0.000 abstract 3
- 239000012190 activator Substances 0.000 abstract 2
- 230000002797 proteolythic effect Effects 0.000 abstract 2
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 230000007026 protein scission Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78298006P | 2006-03-16 | 2006-03-16 | |
| PCT/US2007/064243 WO2007127538A1 (en) | 2006-03-16 | 2007-03-16 | Methods for assaying protein-protein interaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE528647T1 true ATE528647T1 (de) | 2011-10-15 |
Family
ID=38655843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07758759T ATE528647T1 (de) | 2006-03-16 | 2007-03-16 | Verfahren zur untersuchung von protein-protein- interaktionen |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP2002021B1 (de) |
| JP (1) | JP2009529893A (de) |
| AT (1) | ATE528647T1 (de) |
| WO (1) | WO2007127538A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014196932A1 (en) * | 2013-06-06 | 2014-12-11 | Agency For Science, Technology And Research | Protease-responsive peptide biosensors and methods for analyte detection |
| WO2015100058A1 (en) * | 2013-12-23 | 2015-07-02 | Research Corporation Technologies, Inc. | Pichia pastoris surface display system |
| WO2017123559A2 (en) | 2016-01-11 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric proteins and methods of regulating gene expression |
| JP7012645B2 (ja) | 2016-01-11 | 2022-01-28 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | キメラタンパク質および免疫治療の方法 |
| WO2020043812A1 (en) * | 2018-08-30 | 2020-03-05 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Method for detecting a modulator compound for chemical targeting of protein-protein interactions |
| KR20210125476A (ko) * | 2018-12-07 | 2021-10-18 | 옥탄트, 인크. | 단백질-단백질 상호작용 스크리닝을 위한 시스템 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5891646A (en) | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
| US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
| US7128915B2 (en) | 1997-09-18 | 2006-10-31 | Research Development Foundation | Membrane virus host range mutations and their uses as vaccine substrates |
| US6884870B2 (en) | 1998-03-20 | 2005-04-26 | California Institute Of Technology | Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells |
| US6893827B1 (en) * | 2000-02-07 | 2005-05-17 | Applera Corporation | Receptor function assay for G-protein coupled receptors and orphan receptors by reporter enzyme mutant complementation |
| US20030157553A1 (en) | 2000-03-03 | 2003-08-21 | Gabriel Berstein | Methods of assaying for G protein-coupled receptor ligands and modulators |
| EP1205191A1 (de) | 2000-11-13 | 2002-05-15 | Centre National De La Recherche Scientifique | Zielgerichte Änderung von intrazellular Verbindungen |
| US6551786B2 (en) * | 2001-01-04 | 2003-04-22 | Myriad Genetics, Inc. | Screen assay for selecting protein-protein interaction modulators |
| DE10211063A1 (de) * | 2002-03-13 | 2003-10-09 | Axaron Bioscience Ag | Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo |
| EP1353182A3 (de) | 2002-04-12 | 2004-02-04 | Smithkline Beecham Corporation | Verfahren zur Vorhersage von Zell-basierten Assay Resultaten mit Hilfe von Bindungsprofilen |
| CN1894581B (zh) * | 2003-07-09 | 2012-02-01 | 生命技术公司 | 检测蛋白-蛋白相互作用的方法 |
-
2007
- 2007-03-16 JP JP2009500634A patent/JP2009529893A/ja active Pending
- 2007-03-16 WO PCT/US2007/064243 patent/WO2007127538A1/en not_active Ceased
- 2007-03-16 EP EP07758759A patent/EP2002021B1/de not_active Not-in-force
- 2007-03-16 AT AT07758759T patent/ATE528647T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP2002021A4 (de) | 2009-04-22 |
| WO2007127538A1 (en) | 2007-11-08 |
| EP2002021A1 (de) | 2008-12-17 |
| JP2009529893A (ja) | 2009-08-27 |
| EP2002021B1 (de) | 2011-10-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |