ATE542904T1 - Verfahren und mittel zur herstellung von proteinen mit vorbestimmten posttranslationalen modifikationen - Google Patents

Verfahren und mittel zur herstellung von proteinen mit vorbestimmten posttranslationalen modifikationen

Info

Publication number: ATE542904T1
Authority: AT; Austria
Prior art keywords: translational modifications; post; mammalian cells; present; proteinaceous molecules
Prior art date: 2001-10-29

Application number

AT02770322T

Other languages

English (en)

Inventor

Dirk Opstelten

Johan Kapteyn

Petrus Passier

Ronald Brus

Abraham Bout

Original Assignee

Crucell Holland Bv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-10-29

Filing date

2002-10-29

Publication date

2012-02-15

2001-10-29 Priority claimed from PCT/NL2001/000792 external-priority patent/WO2003050286A1/en

2002-04-19 Priority claimed from PCT/NL2002/000257 external-priority patent/WO2003089468A1/en

2002-10-29 Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv

2012-02-15 Application granted granted Critical

2012-02-15 Publication of ATE542904T1 publication Critical patent/ATE542904T1/de

Links

238000000034 method Methods 0.000 title abstract 4
102000004169 proteins and genes Human genes 0.000 title abstract 3
108090000623 proteins and genes Proteins 0.000 title abstract 3
238000012986 modification Methods 0.000 title 1
230000001323 posttranslational effect Effects 0.000 title 1
210000004962 mammalian cell Anatomy 0.000 abstract 5
230000004481 post-translational protein modification Effects 0.000 abstract 5
102000003951 Erythropoietin Human genes 0.000 abstract 3
108090000394 Erythropoietin Proteins 0.000 abstract 3
229940105423 erythropoietin Drugs 0.000 abstract 3
OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 abstract 3
230000013595 glycosylation Effects 0.000 abstract 2
238000006206 glycosylation reaction Methods 0.000 abstract 2
102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
230000001537 neural effect Effects 0.000 abstract 1
229920001542 oligosaccharide Polymers 0.000 abstract 1
150000002482 oligosaccharides Chemical class 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
General Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Genetics & Genomics (AREA)
Biophysics (AREA)
Biomedical Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Wood Science & Technology (AREA)
Neurosurgery (AREA)
Neurology (AREA)
General Engineering & Computer Science (AREA)
Toxicology (AREA)
Immunology (AREA)
Cardiology (AREA)
Heart & Thoracic Surgery (AREA)
Virology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Urology & Nephrology (AREA)
Vascular Medicine (AREA)
Hospice & Palliative Care (AREA)
Psychiatry (AREA)
Pain & Pain Management (AREA)
Rheumatology (AREA)

AT02770322T 2001-10-29 2002-10-29 Verfahren und mittel zur herstellung von proteinen mit vorbestimmten posttranslationalen modifikationen ATE542904T1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
PCT/NL2001/000792 WO2003050286A1 (en)	2001-10-29	2001-10-29	Methods and means for producing proteins with predetermined post-translational modifications
PCT/NL2002/000257 WO2003089468A1 (en)	2002-04-19	2002-04-19	Methods and means for producing proteins with predetermined post-translational modifications
PCT/NL2002/000686 WO2003038100A1 (en)	2001-10-29	2002-10-29	Methods and means for producing proteins with predetermined post-translational modifications

Publications (1)

Publication Number	Publication Date
ATE542904T1 true ATE542904T1 (de)	2012-02-15

Family

ID=26642018

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02770322T ATE542904T1 (de)	2001-10-29	2002-10-29	Verfahren und mittel zur herstellung von proteinen mit vorbestimmten posttranslationalen modifikationen

Country Status (14)

Country	Link
US (3)	US7304031B2 (de)
EP (2)	EP2292770B1 (de)
JP (1)	JP4583029B2 (de)
CN (1)	CN100347306C (de)
AT (1)	ATE542904T1 (de)
AU (1)	AU2002335585B2 (de)
BR (1)	BR0213402A (de)
CA (2)	CA2465007C (de)
DK (1)	DK1440157T3 (de)
EA (2)	EA008220B1 (de)
ES (1)	ES2381104T3 (de)
IL (3)	IL161674A (de)
NO (1)	NO20042209L (de)
WO (1)	WO2003038100A1 (de)

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DE69633565T3 (de)	1995-06-15	2013-01-17	Crucell Holland B.V.	Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
US20050164386A1 (en) *	1999-04-15	2005-07-28	Uytdehaag Alphonsus G.	Overexpression of enzymes involved in post-translational protein modifications in human cells
US7297680B2 (en)	1999-04-15	2007-11-20	Crucell Holland B.V.	Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US6855544B1 (en)	1999-04-15	2005-02-15	Crucell Holland B.V.	Recombinant protein production in a human cell
US7604960B2 (en)	1999-04-15	2009-10-20	Crucell Holland B.V.	Transient protein expression methods
US8236561B2 (en)	1999-04-15	2012-08-07	Crucell Holland B.V.	Efficient production of IgA in recombinant mammalian cells
US7192759B1 (en)	1999-11-26	2007-03-20	Crucell Holland B.V.	Production of vaccines
US7527961B2 (en)	1999-11-26	2009-05-05	Crucell Holland B.V.	Production of vaccines
US7521220B2 (en)	1999-11-26	2009-04-21	Crucell Holland B.V.	Production of vaccines
US20030072737A1 (en)	2000-12-29	2003-04-17	Michael Brines	Tissue protective cytokines for the protection, restoration, and enhancement of responsive cells, tissues and organs
US7767643B2 (en)	2000-12-29	2010-08-03	The Kenneth S. Warren Institute, Inc.	Protection, restoration, and enhancement of erythropoietin-responsive cells, tissues and organs
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ATE384785T1 (de)	2001-12-07	2008-02-15	Crucell Holland Bv	Herstellung von viren, virusisolaten, und impfstoffen
US8524477B2 (en)	2002-10-29	2013-09-03	Crucell Holland B.V.	Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
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EP1954815B1 (de) *	2005-11-15	2015-02-25	GlycoFi, Inc.	Herstellung von glycoproteinen mit reduzierter o-glycosylierung
US7642078B2 (en)	2005-12-28	2010-01-05	Crucell Holland B.V.	Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
US10351615B2 (en)	2006-02-03	2019-07-16	Opko Biologics Ltd.	Methods of treatment with long-acting growth hormone
US7553941B2 (en) *	2006-02-03	2009-06-30	Modigene Inc	Long-acting polypeptides and methods of producing same
US9249407B2 (en)	2006-02-03	2016-02-02	Opko Biologics Ltd.	Long-acting coagulation factors and methods of producing same
US10221228B2 (en)	2006-02-03	2019-03-05	Opko Biologics Ltd.	Long-acting polypeptides and methods of producing and administering same
US20140113860A1 (en)	2006-02-03	2014-04-24	Prolor Biotech Ltd.	Long-acting polypeptides and methods of producing and administering same
US8048849B2 (en)	2006-02-03	2011-11-01	Modigene, Inc.	Long-acting polypeptides and methods of producing same
US8946155B2 (en)	2006-02-03	2015-02-03	Opko Biologics Ltd.	Long-acting polypeptides and methods of producing and administering same
US20150038413A1 (en)	2006-02-03	2015-02-05	Opko Biologics Ltd.	Long-acting polypeptides and methods of producing and administering same
WO2007098548A1 (en) *	2006-03-01	2007-09-07	Apollo Life Sciences Limited	A molecule and chimeric molecules thereof
CN101460188B (zh)	2006-06-07	2013-09-04	国立大学法人德岛大学	使用促红细胞生成素的局部缺血疾病的治疗
EP2057997A4 (de)	2006-08-22	2012-11-07	Chugai Pharmaceutical Co Ltd	Mittel zur prävention und/oder behandlung von peripherer neuropathie
AR065613A1 (es)	2007-03-09	2009-06-17	Chugai Pharmaceutical Co Ltd	Agentes de proteccion para organos transplantados
ES2626080T3 (es)	2007-04-16	2017-07-21	Momenta Pharmaceuticals, Inc.	Caracterización de N-glicanos utilizando exoglicosidasas
TWI488640B (zh)	2008-04-16	2015-06-21	菲瑞茵國際中心股份有限公司	藥學製劑
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US9663778B2 (en)	2009-07-09	2017-05-30	OPKO Biologies Ltd.	Long-acting coagulation factors and methods of producing same
US12203113B2 (en)	2009-07-09	2025-01-21	Opko Biologics Ltd.	Long-acting coagulation factors and methods of producing same
TWI532495B (zh) *	2009-10-05	2016-05-11	菲瑞茵國際中心股份有限公司	藥學製劑
CA3163525A1 (en)	2011-03-31	2012-10-04	Ferring Bv.	Pharmaceutical preparation
UY34317A (es)	2011-09-12	2013-02-28	Genzyme Corp	Anticuerpo antireceptor de célula T (alfa)/ß
EP2773964A4 (de) *	2011-11-03	2015-06-24	Stc Biolog Inc	Verfahren zur bestimmung der pharmakologischen eigenschaften rekombinanter proteine
WO2013157002A1 (en)	2012-04-19	2013-10-24	Prolor Biotech Inc.	Long-acting oxyntomodulin variants and methods of producing same
US9790268B2 (en)	2012-09-12	2017-10-17	Genzyme Corporation	Fc containing polypeptides with altered glycosylation and reduced effector function
SG11201503879QA (en)	2012-11-20	2015-06-29	Opko Biolog Ltd	Method of increasing the hydrodynamic volume of polypeptides by attaching to gonadotrophin carboxy terminal peptides
US9580511B2 (en)	2013-03-11	2017-02-28	Genzyme Corporation	Site-specific antibody-drug conjugation through glycoengineering
US20150158926A1 (en)	2013-10-21	2015-06-11	Opko Biologics, Ltd.	Long-acting polypeptides and methods of producing and administering same
HUE061339T2 (hu)	2014-03-19	2023-06-28	Genzyme Corp	Targetáló csoportok helyspecifikus glikotechnikai átalakítása
CA2954066A1 (en) *	2014-07-01	2016-01-07	Stc Biologics, Inc.	A method for development of recombinant proteins with fingerprint like similarity to the reference product
KR102527180B1 (ko)	2014-12-10	2023-04-27	옵코 바이오로직스 리미티드	장기간 작용성 ctp-변형된 성장 호르몬 폴리펩타이드의 제조 방법
KR102662956B1 (ko)	2015-06-19	2024-05-03	옵코 바이오로직스 리미티드	장기-작용성 응고 인자 및 그의 제조 방법
EP4303228B1 (de)	2016-07-11	2026-05-27	OPKO Biologics Ltd.	Langwirkender gerinnungsfaktor vii und verfahren zur herstellung davon
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CN116440252A (zh) *	2020-06-16	2023-07-18	温州医科大学附属第二医院（温州医科大学附属育英儿童医院）	神经营养因子3在制备提高睾丸中睾酮含量的药物中的应用

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2002
- 2002-10-29 EA EA200400605A patent/EA008220B1/ru not_active IP Right Cessation
- 2002-10-29 CA CA2465007A patent/CA2465007C/en not_active Expired - Fee Related
- 2002-10-29 EP EP10177590.6A patent/EP2292770B1/de not_active Expired - Lifetime
- 2002-10-29 DK DK02770322.2T patent/DK1440157T3/da active
- 2002-10-29 EA EA200602163A patent/EA012340B1/ru not_active IP Right Cessation
- 2002-10-29 WO PCT/NL2002/000686 patent/WO2003038100A1/en not_active Ceased
- 2002-10-29 CN CNB028216903A patent/CN100347306C/zh not_active Expired - Fee Related
- 2002-10-29 BR BR0213402-0A patent/BR0213402A/pt not_active IP Right Cessation
- 2002-10-29 CA CA2756610A patent/CA2756610C/en not_active Expired - Fee Related
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- 2002-10-29 AU AU2002335585A patent/AU2002335585B2/en not_active Ceased
- 2002-10-29 ES ES02770322T patent/ES2381104T3/es not_active Expired - Lifetime
- 2002-10-29 AT AT02770322T patent/ATE542904T1/de active
2004
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2007
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Also Published As

Publication number	Publication date
CA2465007C (en)	2012-01-17
CN100347306C (zh)	2007-11-07
IL161674A (en)	2014-06-30
JP2005507426A (ja)	2005-03-17
EA008220B1 (ru)	2007-04-27
US7785833B2 (en)	2010-08-31
CA2465007A1 (en)	2003-05-08
CN1578838A (zh)	2005-02-09
US7304031B2 (en)	2007-12-04
NO20042209L (no)	2004-07-28
ES2381104T3 (es)	2012-05-23
US7696157B2 (en)	2010-04-13
EP2292770A3 (de)	2012-12-19
IL201674A (en)	2013-10-31
EA200602163A1 (ru)	2007-04-27
WO2003038100A1 (en)	2003-05-08
IL201673A (en)	2013-10-31
EA200400605A1 (ru)	2004-08-26
EP1440157B1 (de)	2012-01-25
DK1440157T3 (da)	2012-05-07
US20050164917A1 (en)	2005-07-28
CA2756610A1 (en)	2003-05-08
EP1440157A1 (de)	2004-07-28
CA2756610C (en)	2015-08-25
AU2002335585B2 (en)	2007-08-16
EP2292770A2 (de)	2011-03-09
BR0213402A (pt)	2004-10-13
EA012340B1 (ru)	2009-10-30
US20070117742A1 (en)	2007-05-24
EP2292770B1 (de)	2014-11-26
US20080032922A1 (en)	2008-02-07
JP4583029B2 (ja)	2010-11-17

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