ATE420061T1 - 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung - Google Patents

13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung

Info

Publication number
ATE420061T1
ATE420061T1 AT98945835T AT98945835T ATE420061T1 AT E420061 T1 ATE420061 T1 AT E420061T1 AT 98945835 T AT98945835 T AT 98945835T AT 98945835 T AT98945835 T AT 98945835T AT E420061 T1 ATE420061 T1 AT E420061T1
Authority
AT
Austria
Prior art keywords
labeled proteins
production
methods
labeled
proteins
Prior art date
Application number
AT98945835T
Other languages
English (en)
Inventor
Frank Anderson
Jonathan Brown
Philip Coughlin
Steven Homans
Charles Weller
Carrie Zimmerman-Wilson
Eddie Oliver
Original Assignee
Martek Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Biosciences Corp filed Critical Martek Biosciences Corp
Application granted granted Critical
Publication of ATE420061T1 publication Critical patent/ATE420061T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B59/00—Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
    • C07B59/008—Peptides; Proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/46—NMR spectroscopy
    • G01R33/465—NMR spectroscopy applied to biological material, e.g. in vitro testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT98945835T 1997-09-02 1998-09-02 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung ATE420061T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/921,554 US6111066A (en) 1997-09-02 1997-09-02 Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains

Publications (1)

Publication Number Publication Date
ATE420061T1 true ATE420061T1 (de) 2009-01-15

Family

ID=25445613

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98945835T ATE420061T1 (de) 1997-09-02 1998-09-02 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung

Country Status (12)

Country Link
US (4) US6111066A (de)
EP (2) EP1009725B1 (de)
JP (1) JP2001514239A (de)
AT (1) ATE420061T1 (de)
AU (1) AU754232B2 (de)
CA (1) CA2301046C (de)
DE (1) DE69840448D1 (de)
HU (1) HUP0003383A3 (de)
NO (1) NO20001052L (de)
TW (1) TW581772B (de)
WO (1) WO1999011589A1 (de)
ZA (1) ZA987975B (de)

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US6111066A (en) 1997-09-02 2000-08-29 Martek Biosciences Corporation Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains
WO2002035239A1 (en) * 2000-10-20 2002-05-02 Martek Biosciences Corporation Rapid determination of protein global folds
US6882939B2 (en) * 2000-10-20 2005-04-19 Prospect Pharma Rapid determination of protein global folds
WO2003002724A2 (en) * 2001-03-12 2003-01-09 Affinium Pharmaceuticals, Inc. Proteins, druggable regions of proteins and target analysis for chemistry of therapeutics
WO2003006488A1 (en) * 2001-07-13 2003-01-23 The Regents Of The University Of California In-cell nmr spectroscopy
JP2003096098A (ja) * 2001-09-25 2003-04-03 Nippon Sanso Corp 安定同位体標識抗体及びその製造方法ならびにこれを用いた検出方法
US20030077572A1 (en) * 2001-10-11 2003-04-24 Abramson Fred P. Quantitative differential display using isotope ratio monitoring
US20040030239A1 (en) * 2001-12-13 2004-02-12 The Johns Hopkins University School Of Medicine Method for MR/NMR imaging
CA2471105C (en) * 2001-12-19 2010-01-12 Japan Science And Technology Agency Stable isotope-labeled amino acid and method for incorporating the same into target protein, nmr method for the structural analysis of protein and method for producing regio-selective stable isotope-labeled fumaric acid and tartaric acid
CA2488773A1 (en) * 2002-06-10 2003-12-18 Prospect Pharma Method for obtaining dynamic and structural data pertaining to proteins and protein/ligand complexes
EP2392659B1 (de) 2002-06-17 2015-01-14 Thrasos Innovation, Inc. TDF-zugehörige Einzeldomänenverbindungen und Analoge davon
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
US7553455B1 (en) * 2003-04-02 2009-06-30 Sandia Corporation Micromanifold assembly
US7672791B2 (en) * 2003-06-13 2010-03-02 International Business Machines Corporation Method of performing three-dimensional molecular superposition and similarity searches in databases of flexible molecules
RU2409657C2 (ru) 2003-07-11 2011-01-20 Татьяна А. ЕГОРОВА-ЗАЧЕРНЮК Композиции и способы для мечения стабильным изотопом биологических соединений
WO2005037853A2 (en) * 2003-10-07 2005-04-28 Prospect Pharma Side chain deuterated amino acids and methods of use
CA2544305C (en) * 2003-10-31 2011-07-12 Japan Science And Technology Agency Stable isotope-labeled aromatic amino acids, method for incorporating the same in target protein and method for nmr-structural analysis of proteins
WO2005073747A1 (ja) * 2004-02-02 2005-08-11 Mitsubishi Chemical Corporation Ｎｍｒシグナル帰属方法
CA2571218C (en) 2004-06-17 2015-11-03 William D. Carlson Tdf-related compounds and analogs thereof
US7372895B2 (en) * 2004-12-08 2008-05-13 Telefonaktiebolaget Lm Ericsson (Publ) Method of and system for delay estimation with minimized finger allocation
US7480356B2 (en) * 2004-12-08 2009-01-20 Telefonaktiebolaget L M Ericsson (Publ) Method of and system for path selection in rich multipath conditions
EP3467096B1 (de) 2005-06-07 2022-01-12 DSM Nutritional Products AG Eukaryotische mikroorganismen zur herstellung von lipiden und antioxidantien
EP2497780B1 (de) 2005-09-20 2015-04-15 Thrasos Innovation, Inc. Verbindungen im Zusammenhang mit TDF und Analoga davon
US20070196280A1 (en) * 2006-02-21 2007-08-23 The Government Of The U.S.A. As Represented By The Sec. Of The Dept. Of Health And Human Services In vivo magnetic resonance spectroscopy of aspartate transaminase activity
GB0604647D0 (en) 2006-03-08 2006-04-19 Shchepinov Mikhail Stabilized food supplements and their derivatives
JP2007255910A (ja) * 2006-03-20 2007-10-04 Hokkaido Univ Ｎｍｒシグナルの帰属方法
ES2572833T3 (es) * 2006-08-01 2016-06-02 Dsm Nutritional Products Ag Proceso de producción de aceite microbiano que contiene ácidos grasos poliinsaturados
DK2276774T3 (en) 2008-03-14 2016-11-28 Retrotope Inc Drugs that modulate genome methylation.
US10029956B2 (en) * 2008-03-14 2018-07-24 Retrotope, Inc. Therapies for cancer using isotopically substituted lysine
EP3536317A1 (de) 2009-10-30 2019-09-11 Retrotope, Inc. Milderung von oxidativen stresserkrankungen mit pufa-derivaten
CN101914030B (zh) * 2010-07-23 2013-04-24 上海化工研究院 一种13c，15n双标记甘氨酸的合成方法
AU2012249920B2 (en) 2011-04-26 2017-06-15 Biojiva Llc Disorders implicating PUFA oxidation
AU2012249918B2 (en) 2011-04-26 2017-06-15 Biojiva Llc Impaired energy processing disorders and mitochondrial deficiency
CA2834274C (en) 2011-04-26 2021-08-03 Retrotope, Inc. Neurodegenerative disorders and muscle diseases implicating pufas
US10058522B2 (en) 2011-04-26 2018-08-28 Retrotope, Inc. Oxidative retinal diseases
US9873880B2 (en) 2013-03-13 2018-01-23 Dsm Nutritional Products Ag Engineering microorganisms
EP3380452B1 (de) 2015-11-23 2021-03-24 Retrotope, Inc. Ortsspezifische isotopische markierung von 1,4-dien-systemen
FR3055337A1 (fr) * 2016-08-24 2018-03-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Methode de preparation de milieux de culture riches pour le marquage d'acides amines incorpores dans des proteines
CN116438187A (zh) 2020-02-21 2023-07-14 拜奥吉瓦有限责任公司 多不饱和脂肪酸及其衍生物的同位素修饰方法
US12109194B2 (en) 2021-02-05 2024-10-08 Biojiva Llc Synergistic combination therapy for treating ALS

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0768446B2 (ja) * 1988-11-09 1995-07-26 三菱化学株式会社 芳香族ポリカーボネート樹脂組成物を用いた医療用部品
JP2518917B2 (ja) 1989-02-07 1996-07-31 日本電子株式会社 蛋白質の構造解析方法
WO1990015525A1 (en) 1989-06-14 1990-12-27 Martek Corporation Media for cell growth and method for making them
JP2517688B2 (ja) * 1989-12-25 1996-07-24 日本電子株式会社 ３次元核磁気共鳴スペクトルの対称化処理方法
JPH0446143A (ja) * 1990-06-11 1992-02-17 Hitachi Ltd 同位体多重標識アミノ酸の合成法
US5393669A (en) * 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
US5891643A (en) 1995-11-14 1999-04-06 Abbott Laboratories Use of nuclear magnetic resonance to design ligands to target biomolecules
US5698401A (en) * 1995-11-14 1997-12-16 Abbott Laboratories Use of nuclear magnetic resonance to identify ligands to target biomolecules
US6043024A (en) 1997-04-18 2000-03-28 Abbott Laboratories Use of one-dimensional nuclear magnetic resonance to identify ligands to target biomolecules
US6111066A (en) 1997-09-02 2000-08-29 Martek Biosciences Corporation Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains

Also Published As

Publication number Publication date
AU754232B2 (en) 2002-11-07
US6111066A (en) 2000-08-29
US6340578B1 (en) 2002-01-22
HUP0003383A3 (en) 2003-07-28
US6335196B1 (en) 2002-01-01
CA2301046A1 (en) 1999-03-11
AU9298898A (en) 1999-03-22
EP1889823A1 (de) 2008-02-20
TW581772B (en) 2004-04-01
US6376253B1 (en) 2002-04-23
DE69840448D1 (de) 2009-02-26
NO20001052D0 (no) 2000-03-01
NO20001052L (no) 2000-04-28
EP1009725B1 (de) 2009-01-07
HUP0003383A2 (hu) 2001-02-28
ZA987975B (en) 2000-01-21
CA2301046C (en) 2009-03-10
EP1009725A1 (de) 2000-06-21
JP2001514239A (ja) 2001-09-11
WO1999011589A1 (en) 1999-03-11

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