ATE545712T1 - Verfahren mit verwendung von poren - Google Patents

Verfahren mit verwendung von poren

Info

Publication number
ATE545712T1
ATE545712T1 AT06808555T AT06808555T ATE545712T1 AT E545712 T1 ATE545712 T1 AT E545712T1 AT 06808555 T AT06808555 T AT 06808555T AT 06808555 T AT06808555 T AT 06808555T AT E545712 T1 ATE545712 T1 AT E545712T1
Authority
AT
Austria
Prior art keywords
nucleotide
pore
relates
pores
identity
Prior art date
Application number
AT06808555T
Other languages
English (en)
Inventor
Hagan Bayley
Yann Astier
Orit Braha
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Application granted granted Critical
Publication of ATE545712T1 publication Critical patent/ATE545712T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Nanotechnology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT06808555T 2005-11-15 2006-11-15 Verfahren mit verwendung von poren ATE545712T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0523282.2A GB0523282D0 (en) 2005-11-15 2005-11-15 Methods using pores
PCT/GB2006/004265 WO2007057668A1 (en) 2005-11-15 2006-11-15 Methods using pores

Publications (1)

Publication Number Publication Date
ATE545712T1 true ATE545712T1 (de) 2012-03-15

Family

ID=35516986

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06808555T ATE545712T1 (de) 2005-11-15 2006-11-15 Verfahren mit verwendung von poren

Country Status (16)

Country Link
US (2) US8105846B2 (de)
EP (2) EP2272981B1 (de)
JP (1) JP5328362B2 (de)
KR (1) KR20080083281A (de)
CN (1) CN101356288B (de)
AT (1) ATE545712T1 (de)
AU (1) AU2006314296B2 (de)
BR (1) BRPI0618391A2 (de)
CA (1) CA2629009C (de)
GB (1) GB0523282D0 (de)
IL (1) IL191319A (de)
MY (1) MY149161A (de)
NZ (1) NZ568163A (de)
RU (1) RU2008123842A (de)
UA (1) UA95087C2 (de)
WO (1) WO2007057668A1 (de)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) * 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP3543357A1 (de) 2007-05-08 2019-09-25 Trustees of Boston University Chemische funktionalisierung von festkörpernanoporen und nanoporenarrays und anwendungen davon
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2307540B1 (de) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzymporenstrukturen
EP2342359B1 (de) * 2008-08-01 2015-03-04 Brown University System und verfahren zur bestimmung von molekülen anhand von massenspektrometrie sowie entsprechende verfahren
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
US20100148126A1 (en) * 2008-11-26 2010-06-17 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
WO2010086622A1 (en) * 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US20120100530A1 (en) * 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0905140D0 (en) * 2009-03-25 2009-05-06 Isis Innovation Method
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
EP2483680A4 (de) 2009-09-30 2014-01-01 Quantapore Inc Ultraschnelle sequenzierung biologischer polymere mithilfe einer markierten nanopore
EP2507387B1 (de) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Vorrichtung und verfahren für biochemische analyse
GB2490846A (en) * 2010-03-05 2012-11-14 Univ Utah Res Found Detection of nucleic acid lesions and adducts using nanopores
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
US8673757B2 (en) 2010-10-28 2014-03-18 International Business Machines Corporation Structure and method for using high-k material as an etch stop layer in dual stress layer process
EP2673638B1 (de) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutante poren
EP3633370B1 (de) 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Verfahren und vorrichtung zur bestimmung der anwesenheit, abwesenheit oder der eigenschaften eines analyten
RU2467218C1 (ru) * 2011-06-16 2012-11-20 Закрытое Акционерное Общество "Научно-производственное объединение "Гидравлические аппараты" (ЗАО "НПО "Гидроаппарат") Гидромуфта
CN103328973B (zh) * 2011-07-20 2015-04-01 加利福尼亚大学董事会 双孔装置
IN2014DN00221A (de) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
EP2987870B1 (de) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Verfahren zur kennzeichnung eines zielpolynukleotids mit einer transmembranpore und eines molekularen motors
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2815235B1 (de) 2012-02-15 2020-04-15 Oxford Nanopore Technologies Limited Aptamer verfahren
WO2013151532A1 (en) * 2012-04-02 2013-10-10 Lux Bio Group, Inc. Apparatus and method for molecular separation, purification, and sensing
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103267785B (zh) * 2013-05-14 2016-04-13 西北大学 一种检测药物包裹或释放过程的单分子分析方法
ES2994071T3 (en) 2013-05-24 2025-01-16 Illumina Cambridge Ltd Pyrophosphorolytic sequencing using nanopores
EP3004385B1 (de) 2013-05-24 2018-11-28 Quantapore Inc. Nanoporenbasierte nukleinsäureanalyse mit gemischtem fret-nachweis
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (de) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Verfahren zur charakterisierung einer doppelsträngiger nukleinsäure mittels einer nanopore und ankermolekülen an beiden enden dieser nukleinsäure
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137627B1 (de) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Verfahren zur verbesserung der bewegung eines zielpolynukleotids bezüglich einer transmembranpore
GB201411285D0 (en) 2014-06-25 2014-08-06 Prosser Joseph Sequencer
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
ES2789000T3 (es) 2014-10-10 2020-10-23 Quantapore Inc Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
CA2966246C (en) 2014-10-31 2020-07-14 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
US10060903B2 (en) * 2014-11-05 2018-08-28 Genia Technologies, Inc. Exporting measurements of nanopore arrays
US9863904B2 (en) 2014-12-19 2018-01-09 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
US9557294B2 (en) 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
AU2016215455A1 (en) * 2015-02-02 2017-08-17 Ontera Inc. Labile linkers for biomarker detection
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) * 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
WO2016166232A1 (en) * 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10126262B2 (en) 2015-09-24 2018-11-13 Genia Technologies, Inc. Differential output of analog memories storing nanopore measurement samples
EP3766987B1 (de) 2015-09-24 2023-08-02 F. Hoffmann-La Roche AG Alpha-hämolysin-varianten
CN105259229B (zh) * 2015-10-22 2018-04-20 清华大学 一种检测药物的单分子分析方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
JP6579948B2 (ja) * 2015-12-24 2019-09-25 株式会社日立ハイテクノロジーズ 生体ポリマを分析するための測定試薬及び分析デバイス
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
EP4122949B1 (de) 2016-04-06 2024-06-05 Oxford Nanopore Technologies plc Mutante pore
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
CN119530363A (zh) * 2017-02-10 2025-02-28 牛津纳米孔科技公开有限公司 修饰的纳米孔、包含其的组合物及其用途
EP4026916B1 (de) 2017-04-19 2023-10-18 Electronic Biosciences Inc. Nanoporen-/nanowell-elektrode mit exonuklease-sequenzierung
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
EP3717905B1 (de) * 2017-11-27 2026-02-11 F. Hoffmann-La Roche AG Normalisierung und grundlinienverschiebungsentfernung für nanopore-sbs-signale
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane
WO2024030919A1 (en) 2022-08-02 2024-02-08 Glyphic Biotechnologies, Inc. Protein sequencing via coupling of polymerizable molecules
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
CA2160909A1 (en) 1993-04-28 1994-11-10 Hagan Bayley Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6267872B1 (en) * 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
AU5631200A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
US7087729B1 (en) 1999-06-29 2006-08-08 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
CA2360342A1 (en) 1999-12-02 2001-06-07 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
EP2083015B1 (de) 2000-02-11 2016-04-06 The Texas A & M University System Biosensorzusammensetzungen und Verfahren zu ihrer Verwendung
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
WO2002101038A1 (fr) * 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
CA2577079C (en) 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
WO2007084103A2 (en) 2004-12-21 2007-07-26 The Texas A & M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) * 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP1963530B1 (de) 2005-12-22 2011-07-27 Pacific Biosciences of California, Inc. Aktive oberflächengekoppelte polymerasen
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
EP3269824A1 (de) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
WO2010004273A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2307540B1 (de) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzymporenstrukturen
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法

Also Published As

Publication number Publication date
UA95087C2 (en) 2011-07-11
US20120219960A1 (en) 2012-08-30
RU2008123842A (ru) 2009-12-27
CA2629009C (en) 2016-02-02
BRPI0618391A2 (pt) 2011-08-30
US8785211B2 (en) 2014-07-22
US8105846B2 (en) 2012-01-31
EP1951898B1 (de) 2012-02-15
EP2272981A1 (de) 2011-01-12
GB0523282D0 (en) 2005-12-21
AU2006314296B2 (en) 2012-08-23
AU2006314296A1 (en) 2007-05-24
JP2009519705A (ja) 2009-05-21
WO2007057668A1 (en) 2007-05-24
CN101356288B (zh) 2016-02-03
CA2629009A1 (en) 2007-05-24
KR20080083281A (ko) 2008-09-17
JP5328362B2 (ja) 2013-10-30
CN101356288A (zh) 2009-01-28
NZ568163A (en) 2011-11-25
EP2272981B1 (de) 2013-01-09
EP1951898A1 (de) 2008-08-06
IL191319A (en) 2011-10-31
MY149161A (en) 2013-07-31
US20080311582A1 (en) 2008-12-18

Similar Documents

Publication Publication Date Title
GB0523282D0 (en) Methods using pores
EP4006169C0 (de) Vorbehandlungsverfahren, vorbehandlungslösung, kit zur detektion von virusnukleinsäuren und verwendung davon
DE602005021336D1 (de) Verfahren zur identifizierung von proteinen mit stärkephosphorylierender enzymatischer aktivität
ATE493513T1 (de) Verfahren zur sequenzierung einer polynukleotidmatrize
ATE511656T1 (de) Verfahren zur risikobewertung von herzeingriffen auf gdf-15-basis
JP2009519705A5 (de)
DE602004017426D1 (de) Benutzung von intronischen rna sequenzen zur quantifizierung der genexpression
ATE515488T1 (de) Click-chemie zur herstellung von reportermolekülen
ATE489482T1 (de) Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
DE10084146D2 (de) Verfahren zur schnellen Bestimmung von Mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
ATE474938T1 (de) Verfahren zur erhöhung der genauigkeit einer nukleinsäuresequenzierung
ATE456676T1 (de) Verfahren zur sequenzierung einer polynukleotidmatrize
ATE441102T1 (de) Verfahren zur detektion von intrazellulären parametern mit lumineszierenden proteinsonden zur selektion von molekülen, die diese parameter ändern kínnen
EP3765478C0 (de) Verfahren zur quantifizierung von rna- und dna-varianten durch sequenzierung unter verwendung von phosphorothioaten
ATE519116T1 (de) Verfahren zur normierung der konzentration von analyten in einer urinprobe
DE60321356D1 (de) Verfahren zur diagnostik von eierstock endometriose
EP3894839C0 (de) Systemtauglichkeitsverfahren zur verwendung bei der bestimmung der proteinkonzentration durch neigung
BRPI0513308A (pt) sistemas e métodos de análise de polìmeros de ácido nucléico e componentes relacionados
ATE529533T1 (de) Verfahren zum nachweis von analyten in einer probe
EP3908677C0 (de) Verfahren zur detektion von nukleinsäuresequenzen
DE602006021131D1 (de) Verfahren zur Bestimmung des Phänotyps von Zellen
ATE456797T1 (de) Verwendung von nogo-c bei der beurteilung von herzversagen
DE602004011228D1 (de) Verfahren und zusammensetzungen zur bestimmung glycierter proteine
DE60232466D1 (de) Verfahren zum testen von weizen
DE60334132D1 (de) Neues verfahren zum testen von nukleinsäure unter verwendung von markiertem nukleotid