ATE554166T1 - Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe - Google Patents

Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe

Info

Publication number
ATE554166T1
ATE554166T1 AT04820001T AT04820001T ATE554166T1 AT E554166 T1 ATE554166 T1 AT E554166T1 AT 04820001 T AT04820001 T AT 04820001T AT 04820001 T AT04820001 T AT 04820001T AT E554166 T1 ATE554166 T1 AT E554166T1
Authority
AT
Austria
Prior art keywords
compositions
methods
rna
present
fixed sample
Prior art date
Application number
AT04820001T
Other languages
English (en)
Inventor
Richard Conrad
Emily Zeringer
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34652241&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE554166(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE554166T1 publication Critical patent/ATE554166T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Enzymes And Modification Thereof (AREA)
AT04820001T 2003-07-25 2004-07-26 Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe ATE554166T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49032503P 2003-07-25 2003-07-25
PCT/US2004/024155 WO2005054466A2 (en) 2003-07-25 2004-07-26 Methods and compositions for preparing rna from a fixed sample

Publications (1)

Publication Number Publication Date
ATE554166T1 true ATE554166T1 (de) 2012-05-15

Family

ID=34652241

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04820001T ATE554166T1 (de) 2003-07-25 2004-07-26 Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe

Country Status (6)

Country Link
US (5) US20050059054A1 (de)
EP (2) EP1654361B2 (de)
JP (1) JP4871722B2 (de)
CN (2) CN1882688B (de)
AT (1) ATE554166T1 (de)
WO (1) WO2005054466A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1601450E (pt) 2003-03-10 2013-09-06 Expression Pathology Inc Preparação líquida de tecido a partir de amostras biológicas, de tecidos e de células tratados histopatologicamente
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
CA2586532C (en) 2004-11-05 2014-03-25 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
US7541144B2 (en) 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US8076064B2 (en) 2005-07-09 2011-12-13 Agilent Technologies, Inc. Method of treatment of RNA sample
US7718365B2 (en) 2005-07-09 2010-05-18 Agilent Technologies, Inc. Microarray analysis of RNA
SI1924252T1 (sl) * 2005-07-28 2011-12-30 Id Fish Technology Inc Metoda za izboljšanje celične permeabilnosti za tuje partikle
US8758990B2 (en) * 2005-07-28 2014-06-24 Id-Fish Technology, Inc. Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US7700289B2 (en) 2006-04-03 2010-04-20 Agilent Technologies, Inc. Method for labeling RNA
US8163535B2 (en) * 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
DE102006031764B4 (de) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren
EP2071313A4 (de) 2006-09-28 2012-10-31 Shimadzu Corp Verfahren zur entparaffinisierung von in paraffin eingebetteten präparaten und verfahren zur analyse von in paraffin eingebetteten präparaten
TW200911988A (en) 2007-06-22 2009-03-16 Response Genetics Inc Methods for isolating long fragment RNA from fixed samples
MY157651A (en) * 2007-07-23 2016-07-15 Yiap Beow Chin A method, kit and apparatus for extracting biological materials
US20090035748A1 (en) * 2007-08-01 2009-02-05 Lizzi Tiffiny Marie Bromelain as a clinical sample pre-treatment, lysis agent and nuclease inhibitor
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
EP2208785A1 (de) 2009-01-15 2010-07-21 Newbiotechnic, S.A. Verfahren und Kits zum Erzeugen von miRNA- und smallRNA-Expressionsvektoren und ihre Anwendung zur Entwicklung von lentiviralen Expressionsbibliotheken
DE102009025574A1 (de) * 2009-06-19 2010-12-23 Leica Biosystems Nussloch Gmbh Verfahren zum automatischen Bearbeiten von Gewebeproben in einem Gewebeprozessor
US20110027862A1 (en) * 2009-06-29 2011-02-03 Life Technologies Corporation Sample stabilization
EP2539449B9 (de) * 2010-02-26 2018-09-05 Qiagen GmbH Verfahren zur parallelen isolierung und/oder aufreinigung von rna und dna
CN101914523B (zh) * 2010-07-08 2013-04-24 浙江理工大学 从人血清/血浆样本中分离rna方法及其pcr验证法
CN101935648B (zh) * 2010-09-13 2013-04-17 原平皓(天津)生物技术有限公司 一种提取rna的方法和试剂盒
US20120149088A1 (en) 2010-11-30 2012-06-14 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
EP2505664B1 (de) * 2011-03-30 2014-12-03 Universität Leipzig Verfahren und Mittel zur Unterscheidung von gut- und bösartigen Tumorproben, insbesondere bei der FNAB
US20140243216A1 (en) 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
WO2013115476A1 (ko) * 2012-01-31 2013-08-08 국립암센터 검체 시료의 응집용 조성물, 이를 이용한 파라핀 블록의 제작방법 및 상기 파라핀 블록을 이용한 세포 마이크로어레이용 세포군집 표본의 제조방법
US20150225712A1 (en) * 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
US10233508B2 (en) 2012-08-21 2019-03-19 Qiagen Gmbh Virus particle stabilisation and method for isolating viral nucleic acids
WO2014033326A1 (en) * 2012-09-03 2014-03-06 Qiagen Gmbh Method for isolating rna including small rna with high yield
GB201217405D0 (en) 2012-09-28 2012-11-14 Fermentas Uab Protein removal agent
EP3670663B1 (de) 2014-02-28 2024-08-07 Gen-Probe Incorporated Verfahren zur isolierung von nukleinsäure aus proben in flüssigkeitsbasierten zytologischen konservierungsmitteln mit formaldehyd
US9771571B2 (en) 2014-02-28 2017-09-26 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
US9771572B2 (en) 2014-02-28 2017-09-26 Gen-Probe Incorporated Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
GB2559919B (en) 2014-02-28 2018-11-14 Gen Probe Inc Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
US10954507B2 (en) 2014-07-17 2021-03-23 Qiagen Gmbh Method for isolating RNA with high yield
SG10201505563XA (en) * 2015-07-15 2017-02-27 Delta Electronics Int’L Singapore Pte Ltd Nucleic acid extraction method
CN105063014A (zh) * 2015-07-28 2015-11-18 福建师范大学 一种利用吸附柱提取植物MicroRNA的方法
HUE062204T2 (hu) 2017-01-16 2023-10-28 Spectrum Solutions L L C Nukleinsavkonzerváló oldat és eljárások alkalmazására
CN111148834B (zh) 2017-09-27 2024-06-11 凯杰有限公司 以高收率分离rna的方法
CN112522792B (zh) * 2020-01-22 2022-12-20 微岩医学科技(北京)有限公司 一种rna测序文库的构建方法
EP4193137B1 (de) * 2020-08-10 2026-03-18 Leica Biosystems Nussloch GmbH Xylolfreie gewebeaufbereitungsvorrichtung und verfahren zur verarbeitung von gewebe
KR102291738B1 (ko) * 2021-01-15 2021-08-20 (주)진이어스 컬럼 방식의 단일튜브 pcr용 핵산 분리를 위한 버퍼 조성물 및 이의 용도
CN114426970A (zh) * 2022-03-17 2022-05-03 黑龙江省科学院高技术研究院 一种适合杨树rna的提取方法
CN115261368A (zh) * 2022-08-16 2022-11-01 北京思尔成生物技术有限公司 一种即用型胃蛋白酶工作液及其制备方法和应用

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US4946669A (en) 1987-10-09 1990-08-07 Siegfried Barry A Histological fixatives
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
JP2846018B2 (ja) 1988-01-21 1999-01-13 ジェネンテク,インコーポレイテッド 核酸配列の増幅および検出
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5260048A (en) * 1991-05-08 1993-11-09 Streck Laboratories, Inc. Tissue fixative solution and method
US5196182A (en) 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
AU3728093A (en) 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
DE69433425T2 (de) * 1993-08-30 2004-10-07 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
JP3256238B2 (ja) 1994-02-11 2002-02-12 クイアジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 二本鎖/単鎖核酸構造物の分離方法
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
AU707391B2 (en) 1994-05-28 1999-07-08 Tepnel Medical Limited Producing copies of nucleic acids
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US6593086B2 (en) 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US20070269799A9 (en) 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
CA2195562A1 (en) 1994-08-19 1996-02-29 Pe Corporation (Ny) Coupled amplification and ligation method
US5935825A (en) 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
JP2965131B2 (ja) * 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US6284535B1 (en) 1995-09-07 2001-09-04 The Trustees Of Columbia University In The City Of New York Splice variants of the heregulin gene, nARIA and uses thereof
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6168918B1 (en) * 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
DE29601618U1 (de) * 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
EP0818461B1 (de) * 1996-07-12 2005-09-28 Toyo Boseki Kabushiki Kaisha Verfahren zur Isolierung von Ribonucleinsäuren.
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
WO1998012354A1 (en) 1996-09-19 1998-03-26 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
EP1023460A4 (de) * 1997-06-25 2003-09-10 Promega Corp Verfahren zur isolierung von rna
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
DE19743518A1 (de) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
US6111096A (en) * 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6387695B1 (en) * 1997-12-23 2002-05-14 Merck & Co., Inc. DNA pharmaceutical formulations comprising citrate or triethanolamine and combinations thereof
US6324479B1 (en) 1998-05-08 2001-11-27 Rosetta Impharmatics, Inc. Methods of determining protein activity levels using gene expression profiles
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6316193B1 (en) 1998-10-06 2001-11-13 Origene Technologies, Inc. Rapid-screen cDNA library panels
US6664379B1 (en) 1999-09-24 2003-12-16 Ambion, Inc. Nuclease inhibitor cocktail
WO2001029265A1 (en) * 1999-10-15 2001-04-26 Ventana Medical Systems, Inc. Method of detecting single gene copies in-situ
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
AU6380001A (en) 2000-03-24 2001-10-03 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
DE10033991A1 (de) 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
GB2369822A (en) 2000-12-05 2002-06-12 Genovar Diagnostics Ltd Nucleic acid extraction method and kit
CN1373136A (zh) * 2001-03-06 2002-10-09 耿东进 快速分离,纯化核糖核酸(rna)的试剂及方法
US6509175B2 (en) 2001-04-11 2003-01-21 Incyte Genomics, Inc. cDNA libraries and methods for their production
WO2003046146A2 (en) * 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
WO2003057910A2 (en) * 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
DK2258872T3 (da) 2002-03-13 2013-11-18 Genomic Health Inc Genekspressionsprofilering i biopsier af tumorvæv
US7364846B2 (en) 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives

Also Published As

Publication number Publication date
CN1882688B (zh) 2014-07-16
EP1654361B1 (de) 2012-04-18
CN1878866A (zh) 2006-12-13
US20140329703A1 (en) 2014-11-06
US20100035770A1 (en) 2010-02-11
US20200109440A1 (en) 2020-04-09
EP1654361A2 (de) 2006-05-10
CN1882688A (zh) 2006-12-20
WO2005054466A3 (en) 2005-12-15
WO2005054466A2 (en) 2005-06-16
CN1878866B (zh) 2012-08-01
EP2295569A1 (de) 2011-03-16
JP4871722B2 (ja) 2012-02-08
EP1654361B2 (de) 2023-01-18
US20160333394A1 (en) 2016-11-17
US20050059054A1 (en) 2005-03-17
JP2007500009A (ja) 2007-01-11

Similar Documents

Publication Publication Date Title
WO2005054466A3 (en) Methods and compositions for preparing rna from a fixed sample
ATE547537T1 (de) Verfahren zur bestimmung der dna-methylierung in blut- oder urinproben
DE60234464D1 (de) Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
ATE430812T1 (de) Materialien und verfahren zum einfangen von krankheitserregern und zur abtrennung von aurintricarbonsäure aus einer probe
ATE349518T1 (de) Verfahren zur isolierung von rns aus fixierten paraffin-eingebetteten gewebeproben
DE60134436D1 (de) Lebensmittel für Diabetiker und Verfahren zur Herstellung desselben
DE60209193D1 (de) Verfahren zur Identifizierung von Adeno-assoziiertem Virus (AAV) Sequenzen sowie Kit zur Ausführung der Methode
EP2446892A3 (de) Isolierte adulte pluripotente Stammzellen und Verfahren zu deren Isolierung und Kultivierung
ATE458506T1 (de) Knochenmarkersatzmaterial-verbundwerkstoff, verfahren zu seiner herstelllung und kit
EP1585825A4 (de) Zusammensetzungen und verfahren zur nukleinsäureextraktion aus biologischen proben
DE60216389D1 (de) Verfahren zur zerstörungsfreien Prüfung
TW200745556A (en) Biomarkers for liver fibrotic injury
DE602004018971D1 (de) Verfahren zur gewinnung von polyhydroxyalkanolaten ( phas ) aus zellbiomasse
DE60226783D1 (de) Mikrofluidische Vorrichtung, Verfahren zur Herstellung und Verwendung desselben
DE60126943D1 (de) Kaelteresistente fettzusammensetzung sowie verfahren zur herstellung derselben
ATE406809T1 (de) Zusammensetzungen enthaltend milchproteinkonzentrat und fettsäure und verfahren zur herstellung
DE60218988D1 (de) Verfahren zur bakterienunterstützten haldenauslaugung von chalkopyrit
DE69932597D1 (de) Verfahren zur reinigung von prpres aus einem biologischen muster und dessen verwendung
DE50204289D1 (de) Vorrichtung und verfahren zur selektion von selbstbeweglichen biologischen spezies, insbesondere von samenzellen
DE60326327D1 (de) Verfahren und zusammensetzungen zur identifikation und charakterisierung von hepatitis c
ATE440145T1 (de) Verfahren zur produktion von plasminogen-kringle 2 plus serine protease domäne (k2s) im grossmassstab in prokaryonten
WO2009125936A3 (ko) 다낭성 난소 증후군 진단을 위한 분석방법 및 키트
DE60132549D1 (de) Verfahren zur Herstellung eines Legierungspulvers für eine Elektrode
DE60141221D1 (de) Verfahren zur genexpressionsanalyse
DE60108746D1 (de) Verfahren zur bestimmung der lipid-aufnahme