ATE59410T1 - Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer. - Google Patents

Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer.

Info

Publication number: ATE59410T1
Authority: AT; Austria
Prior art keywords: production; expression vectors; tpa; methods; human tpa
Prior art date: 1983-01-19

Application number

AT84300299T

Other languages

English (en)

Inventor

Arthur David Levinson

Christian Clinton Simonsen

Elizabeth Mcleod Yelverton

Original Assignee

Genentech Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-01-19

Filing date

1984-01-18

Publication date

1991-01-15

1984-01-18 Application filed by Genentech Inc filed Critical Genentech Inc

1991-01-15 Application granted granted Critical

1991-01-15 Publication of ATE59410T1 publication Critical patent/ATE59410T1/de

Links

239000013604 expression vector Substances 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
102000003978 Tissue Plasminogen Activator Human genes 0.000 abstract 3
108090000373 Tissue Plasminogen Activator Proteins 0.000 abstract 3
229960000187 tissue plasminogen activator Drugs 0.000 abstract 3
102100024746 Dihydrofolate reductase Human genes 0.000 abstract 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 abstract 1
108020001096 dihydrofolate reductase Proteins 0.000 abstract 1
229960000485 methotrexate Drugs 0.000 abstract 1
238000003199 nucleic acid amplification method Methods 0.000 abstract 1
101150010939 tpa gene Proteins 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Magnetic Resonance Imaging Apparatus (AREA)
Electrotherapy Devices (AREA)
Ultra Sonic Daignosis Equipment (AREA)
Saccharide Compounds (AREA)
Peptides Or Proteins (AREA)

AT84300299T 1983-01-19 1984-01-18 Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer. ATE59410T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US45915383A	1983-01-19	1983-01-19
EP84300299A EP0117059B1 (de)	1983-01-19	1984-01-18	Verfahren zur Herstellung von menschlichem tPA und Expressionsvektoren dafür

Publications (1)

Publication Number	Publication Date
ATE59410T1 true ATE59410T1 (de)	1991-01-15

Family

ID=23823628

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT84300299T ATE59410T1 (de)	1983-01-19	1984-01-18	Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer.

Country Status (12)

Country	Link
EP (1)	EP0117059B1 (de)
JP (1)	JPS59183693A (de)
KR (1)	KR840007255A (de)
AT (1)	ATE59410T1 (de)
AU (1)	AU572108B2 (de)
CA (1)	CA1271149A (de)
DE (1)	DE3483839D1 (de)
DK (1)	DK174081B1 (de)
ES (1)	ES529023A0 (de)
IE (1)	IE56716B1 (de)
MY (1)	MY101958A (de)
ZA (1)	ZA84375B (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU2353384A (en) *	1983-01-19	1984-07-26	Genentech Inc.	Amplification in eukaryotic host cells
US4713339A (en) *	1983-01-19	1987-12-15	Genentech, Inc.	Polycistronic expression vector construction
US4703008A (en) *	1983-12-13	1987-10-27	Kiren-Amgen, Inc.	DNA sequences encoding erythropoietin
NZ210501A (en) *	1983-12-13	1991-08-27	Kirin Amgen Inc	Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
KR850004274A (ko) *	1983-12-13	1985-07-11	원본미기재	에리트로포이에틴의 제조방법
GB8415186D0 (en) *	1984-06-14	1984-07-18	Ciba Geigy Ag	Polypeptides
EP0174835A1 (de) *	1984-09-11	1986-03-19	The Upjohn Company	Menschlicher Gewebeplasminogenaktivator und rekombinante DNA-Zusammensetzungen
NZ213647A (en) *	1984-10-01	1989-05-29	Integrated Genetics Inc	Recombinant human tissue plasminogen activator (tpa) modified in n-linked glycosylation pattern
DK149786A (da) *	1985-04-04	1986-10-04	Beecham Group Plc	Proteolytisk enzym
US5672502A (en) *	1985-06-28	1997-09-30	Celltech Therapeutics Limited	Animal cell culture
AU593264B2 (en) *	1985-07-10	1990-02-08	Kanegafuchi Kagaku Kogyo Kabushiki Kaisha	Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
NZ217331A (en) *	1985-08-26	1989-05-29	Lilly Co Eli	Tissue plasminogen activator derivatives and genetically engineered product
GB8528321D0 (en) *	1985-11-18	1985-12-24	Ciba Geigy Ag	Modified fibrinolytic agents
JPH0714341B2 (ja) *	1985-11-20	1995-02-22	鐘淵化学工業株式会社	蛋白質の高生産株の取得方法
US4851517A (en) *	1986-02-26	1989-07-25	Monsanto Company	Tissue plasminogen activator oligosaccharide from normal human colon cells
US4927630A (en) *	1986-02-26	1990-05-22	Monsanto Company	Tissue plasminogen activator from normal human colon cells
US4751084A (en) *	1986-02-26	1988-06-14	Monsanto Company	Tissue plasminogen activator from normal human colon cells
US5132214A (en) *	1986-04-09	1992-07-21	Monsanto Company	Large scale production of plasminogen activator from normal human colon cells
CA1297434C (en) *	1986-04-14	1992-03-17	Kenji Murakami	Method of producing peptides, recombinant plasmid for use in the same and animal cells transformed with the same
JP2585532B2 (ja) *	1986-05-10	1997-02-26	三井東圧化学株式会社	動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
JPS62265992A (ja) *	1986-05-12	1987-11-18	Ajinomoto Co Inc	ヒト細胞を用いる有用物質の製造方法
PT84991B (pt) *	1986-06-06	1990-03-08	Genentech Inc	Processo para a producao de actividador do plasminogenio biologicamente activo
JPS63105675A (ja) *	1986-10-23	1988-05-10	Green Cross Corp:The	ヒトウロキナ−ゼの製造方法
PT86162B (pt) *	1986-11-21	1990-11-20	Smithkline Beecham Corp	Expressao de proteina recombinante
ZA879286B (en)	1986-12-16	1988-10-26	Smith Kline Rit	New plasminogen activators
EP0281246A3 (de) *	1987-02-04	1989-07-12	Invitron Corporation	Verfahren zur Erhöhung der Amplifikation und Expression fremder Gene
NZ219516A (en) *	1987-02-10	1991-02-26	Wellcome Found	Fusion proteins of hbcag (hepatitis b virus core antigen)
US5215896A (en) *	1987-03-20	1993-06-01	Creative Biomolecules, Inc.	Leader sequences for the production of recombinant proteins
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5681713A (en) *	1987-05-08	1997-10-28	Smithkline Beecham Corporation	Expression of heterologous proteins in Drosophila cells
CA1341345C (en) *	1987-05-08	2002-03-05	Hanne Ranch Johansen	Expression of foreign genes in drosophila cells
US4873316A (en) *	1987-06-23	1989-10-10	Biogen, Inc.	Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5017478A (en) *	1987-07-16	1991-05-21	Berlex Laboratories, Inc.	Transfected cells containing plasmids having genes oriented in opposing directions and methods of using
ATE87845T1 (de) *	1987-12-21	1993-04-15	Unilever Nv	Nickel-kieselerde-katalysatoren und ihre bereitung.
EP0555203B1 (de) *	1988-12-08	1995-10-18	Abbott Laboratories	Verfahren zur induzierbaren genexpression unter verwendung eines dhfr-gens
US5641670A (en) *	1991-11-05	1997-06-24	Transkaryotic Therapies, Inc.	Protein production and protein delivery
US5733761A (en) *	1991-11-05	1998-03-31	Transkaryotic Therapies, Inc.	Protein production and protein delivery
TW402639B (en) *	1992-12-03	2000-08-21	Transkaryotic Therapies Inc	Protein production and protein delivery
CA2716766A1 (en)	2008-02-27	2009-09-03	Fuso Pharmaceutical Industries, Ltd.	Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
CN102325881B (zh)	2008-12-22	2014-08-20	国立大学法人北海道大学	用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
AU2010282733B2 (en)	2009-08-11	2016-07-14	Genentech, Inc.	Production of proteins in glutamine-free cell culture media
RU2500817C1 (ru) *	2012-07-19	2013-12-10	Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум")	РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рВК415, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 1F8 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ И ВЫДЕЛЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА
KR102307278B1 (ko)	2014-04-29	2021-09-30	노파르티스 아게	신규 척추동물 세포 및 관심 폴리펩티드의 재조합적 발현 방법
JP7264353B2 (ja)	2018-05-24	2023-04-25	国立大学法人北海道大学	新規ベクターおよびその利用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4370417A (en) *	1980-04-03	1983-01-25	Abbott Laboratories	Recombinant deoxyribonucleic acid which codes for plasminogen activator
JPS5879205A (ja) *	1981-11-06	1983-05-13	Canon Inc	色分解用フイルタ−の製造方法
AR241654A1 (es) *	1982-05-05	1992-10-30	Genentech Inc	Procedimiento para producir activador de plasminogeno de tejido humano.
AU2353384A (en) *	1983-01-19	1984-07-26	Genentech Inc.	Amplification in eukaryotic host cells
JPS61185427A (ja) *	1985-02-13	1986-08-19	Toyoda Gosei Co Ltd	射出装置
JPS6423654A (en) *	1987-07-17	1989-01-26	Fujitsu Ltd	Pre-emphasis circuit

1984
- 1984-01-17 IE IE97/84A patent/IE56716B1/en not_active IP Right Cessation
- 1984-01-17 AU AU23534/84A patent/AU572108B2/en not_active Expired
- 1984-01-18 EP EP84300299A patent/EP0117059B1/de not_active Expired - Lifetime
- 1984-01-18 DK DK198400211A patent/DK174081B1/da not_active IP Right Cessation
- 1984-01-18 DE DE8484300299T patent/DE3483839D1/de not_active Expired - Lifetime
- 1984-01-18 AT AT84300299T patent/ATE59410T1/de not_active IP Right Cessation
- 1984-01-18 ZA ZA84375A patent/ZA84375B/xx unknown
- 1984-01-19 KR KR1019840000220A patent/KR840007255A/ko not_active Ceased
- 1984-01-19 CA CA000445592A patent/CA1271149A/en not_active Expired - Lifetime
- 1984-01-19 ES ES529023A patent/ES529023A0/es active Granted
- 1984-01-19 JP JP59007898A patent/JPS59183693A/ja active Pending
1988
- 1988-08-04 MY MYPI88000886A patent/MY101958A/en unknown

Also Published As

Publication number	Publication date
AU572108B2 (en)	1988-05-05
EP0117059A2 (de)	1984-08-29
EP0117059A3 (en)	1986-03-05
DK174081B1 (da)	2002-05-27
DK21184D0 (da)	1984-01-18
ZA84375B (en)	1985-02-27
CA1271149A (en)	1990-07-03
IE840097L (en)	1984-07-19
AU2353484A (en)	1984-07-26
ES8507610A1 (es)	1985-10-01
IE56716B1 (en)	1991-11-20
KR840007255A (ko)	1984-12-06
JPS59183693A (ja)	1984-10-18
DK21184A (da)	1984-08-24
EP0117059B1 (de)	1990-12-27
MY101958A (en)	1992-02-15
DE3483839D1 (de)	1991-02-07
ES529023A0 (es)	1985-10-01

Legal Events

Date	Code	Title	Description
1991-08-15	UEP	Publication of translation of european patent specification
2004-04-15	EELA	Cancelled due to lapse of time

Publication	Publication Date	Title
ATE59410T1 (de)	1991-01-15	Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer.
DE68928934D1 (de)	1999-04-01	Verfahren zur herstellung von menschlicher dnase
DE3479321D1 (en)	1989-09-14	Biosynthetically produced human nerve growth factor, process for producing it, compositions containing it, dna sequence encoding it, vectors containing the sequence and host cells transformed thereby
KR850007275A (ko)	1985-12-02	기능성 인체인자 ⅷ의 제조방법
ATE69466T1 (de)	1991-11-15	Superoxyddismutase.
ATE162551T1 (de)	1998-02-15	Verfahren zur herstellung von dihomo-gamma- linolensäure und das in dieser enthaltende lipid
ATE153696T1 (de)	1997-06-15	Verfahren und desoxyribonukleinsäure zur herstellung von gewebefaktor-protein
DE3778758D1 (de)	1992-06-11	Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.
ATE107362T1 (de)	1994-07-15	Polypeptid-mutanten des menschlichen tnf und für diese mutanten codierende dns.
ATE70301T1 (de)	1991-12-15	Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
DE3872049D1 (de)	1992-07-23	Alkohol, fett und protein enthaltendes aufschaumbares produkt sowie verfahren zu seiner herstellung.
DE3869230D1 (de)	1992-04-23	Verfahren zur herstellung einer loesung hoher spezifischer volumenaktivitaet von einem protein mit gewebe-plasminogenaktivator (t-pa)-aktivitaet, loesung, enthaltend protein mit t-pa-aktivitaet und verwendung der loesung in der human- und veterinaermedizin.
HUT56884A (en)	1991-10-28	Process for producing protein having urate oxidase activity, gene coding for it, expression vector, microorganisms and transformed cells
ATE67242T1 (de)	1991-09-15	Verfahren zur verbesserung der expression von eukaryontenprotein.
DE69328992D1 (de)	2000-08-17	Mutanten von menschlichem Antithrombin III
SE8702562D0 (sv)	1987-06-18	Novel fibrinolytic enzymes
DE68907166D1 (de)	1993-07-22	Verfahren zur herstellung von menschlichem lysozym.
ATE102994T1 (de)	1994-04-15	Verfahren zur herstellung von proteinen.
DE3783299D1 (de)	1993-02-11	Herstellung von mikroorganismen mit eiskristallbildungsaktivitaet.
DE68928634D1 (de)	1998-05-14	Verfahren zur Herstellung von aktivierten Killer-Monozyten und Verfahren zur Überprüfung ihrer tumoriziden Aktivität
DE3766667D1 (de)	1991-01-24	Verfahren zur herstellung von homo- und copolymeren des vinylchlorids in emulsion oder mikrosuspension.
DE3486168D1 (de)	1993-07-22	Verfahren zur herstellung von l-glutaminsaeure durch fermentation und dabei zu verwendende mutante mikroorganismen.
ATE48531T1 (de)	1989-12-15	Verfahren zum sterilisieren von rekombinantem mikroorganismus.
DE58902277D1 (de)	1992-10-22	Verfahren zur herstellung von gewuerzzubereitungen.
DE68920269D1 (de)	1995-02-09	Verfahren zur Herstellung des menschlichen Gewebeplasminogenaktivators.