ATE59410T1 - Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer. - Google Patents
Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer.Info
- Publication number
- ATE59410T1 ATE59410T1 AT84300299T AT84300299T ATE59410T1 AT E59410 T1 ATE59410 T1 AT E59410T1 AT 84300299 T AT84300299 T AT 84300299T AT 84300299 T AT84300299 T AT 84300299T AT E59410 T1 ATE59410 T1 AT E59410T1
- Authority
- AT
- Austria
- Prior art keywords
- production
- expression vectors
- tpa
- methods
- human tpa
- Prior art date
Links
- 239000013604 expression vector Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 abstract 3
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 abstract 3
- 229960000187 tissue plasminogen activator Drugs 0.000 abstract 3
- 102100024746 Dihydrofolate reductase Human genes 0.000 abstract 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 abstract 1
- 108020001096 dihydrofolate reductase Proteins 0.000 abstract 1
- 229960000485 methotrexate Drugs 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 101150010939 tpa gene Proteins 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Electrotherapy Devices (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45915383A | 1983-01-19 | 1983-01-19 | |
| EP84300299A EP0117059B1 (de) | 1983-01-19 | 1984-01-18 | Verfahren zur Herstellung von menschlichem tPA und Expressionsvektoren dafür |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE59410T1 true ATE59410T1 (de) | 1991-01-15 |
Family
ID=23823628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT84300299T ATE59410T1 (de) | 1983-01-19 | 1984-01-18 | Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer. |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0117059B1 (de) |
| JP (1) | JPS59183693A (de) |
| KR (1) | KR840007255A (de) |
| AT (1) | ATE59410T1 (de) |
| AU (1) | AU572108B2 (de) |
| CA (1) | CA1271149A (de) |
| DE (1) | DE3483839D1 (de) |
| DK (1) | DK174081B1 (de) |
| ES (1) | ES8507610A1 (de) |
| IE (1) | IE56716B1 (de) |
| MY (1) | MY101958A (de) |
| ZA (1) | ZA84375B (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4713339A (en) * | 1983-01-19 | 1987-12-15 | Genentech, Inc. | Polycistronic expression vector construction |
| AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
| US4703008A (en) * | 1983-12-13 | 1987-10-27 | Kiren-Amgen, Inc. | DNA sequences encoding erythropoietin |
| NZ210501A (en) * | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| KR850004274A (ko) * | 1983-12-13 | 1985-07-11 | 원본미기재 | 에리트로포이에틴의 제조방법 |
| GB8415186D0 (en) * | 1984-06-14 | 1984-07-18 | Ciba Geigy Ag | Polypeptides |
| EP0174835A1 (de) * | 1984-09-11 | 1986-03-19 | The Upjohn Company | Menschlicher Gewebeplasminogenaktivator und rekombinante DNA-Zusammensetzungen |
| DK175327B1 (da) * | 1984-10-01 | 2004-08-23 | Integrated Genetics Inc | Replicerbar ekspressionsvektor og rekombinant DNA-molekyle, som indkoder aktiv human livmodervævsplasminogen-aktivator, pattedyrscelle transformeret med vektoren og fremgangsmåde til fremstilling af aktiv human vævsplasminogen-aktivator (TPA) |
| GR860875B (en) * | 1985-04-04 | 1986-07-29 | Beecham Group Plc | Novel compounds |
| US5672502A (en) * | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| AU593264B2 (en) * | 1985-07-10 | 1990-02-08 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor |
| NZ217331A (en) * | 1985-08-26 | 1989-05-29 | Lilly Co Eli | Tissue plasminogen activator derivatives and genetically engineered product |
| GB8528321D0 (en) * | 1985-11-18 | 1985-12-24 | Ciba Geigy Ag | Modified fibrinolytic agents |
| JPH0714341B2 (ja) * | 1985-11-20 | 1995-02-22 | 鐘淵化学工業株式会社 | 蛋白質の高生産株の取得方法 |
| US5132214A (en) * | 1986-04-09 | 1992-07-21 | Monsanto Company | Large scale production of plasminogen activator from normal human colon cells |
| US4751084A (en) * | 1986-02-26 | 1988-06-14 | Monsanto Company | Tissue plasminogen activator from normal human colon cells |
| US4927630A (en) * | 1986-02-26 | 1990-05-22 | Monsanto Company | Tissue plasminogen activator from normal human colon cells |
| US4851517A (en) * | 1986-02-26 | 1989-07-25 | Monsanto Company | Tissue plasminogen activator oligosaccharide from normal human colon cells |
| CA1297434C (en) * | 1986-04-14 | 1992-03-17 | Kenji Murakami | Method of producing peptides, recombinant plasmid for use in the same and animal cells transformed with the same |
| JP2585532B2 (ja) * | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| JPS62265992A (ja) * | 1986-05-12 | 1987-11-18 | Ajinomoto Co Inc | ヒト細胞を用いる有用物質の製造方法 |
| IL82746A (en) * | 1986-06-06 | 1994-10-07 | Genentech Inc | Process for producing biologically active tissue-type plasminogen activator |
| JPS63105675A (ja) * | 1986-10-23 | 1988-05-10 | Green Cross Corp:The | ヒトウロキナ−ゼの製造方法 |
| PT86162B (pt) * | 1986-11-21 | 1990-11-20 | Smithkline Beecham Corp | Expressao de proteina recombinante |
| ZA879286B (en) | 1986-12-16 | 1988-10-26 | Smith Kline Rit | New plasminogen activators |
| EP0281246A3 (de) * | 1987-02-04 | 1989-07-12 | Invitron Corporation | Verfahren zur Erhöhung der Amplifikation und Expression fremder Gene |
| NZ219516A (en) * | 1987-02-10 | 1991-02-26 | Wellcome Found | Fusion proteins of hbcag (hepatitis b virus core antigen) |
| US5013653A (en) * | 1987-03-20 | 1991-05-07 | Creative Biomolecules, Inc. | Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage |
| US5215896A (en) * | 1987-03-20 | 1993-06-01 | Creative Biomolecules, Inc. | Leader sequences for the production of recombinant proteins |
| CA1341345C (en) * | 1987-05-08 | 2002-03-05 | Hanne Ranch Johansen | Expression of foreign genes in drosophila cells |
| US5681713A (en) * | 1987-05-08 | 1997-10-28 | Smithkline Beecham Corporation | Expression of heterologous proteins in Drosophila cells |
| US4873316A (en) * | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
| US5017478A (en) * | 1987-07-16 | 1991-05-21 | Berlex Laboratories, Inc. | Transfected cells containing plasmids having genes oriented in opposing directions and methods of using |
| DE3880113T2 (de) * | 1987-12-21 | 1993-07-15 | Unilever Nv | Nickel-kieselerde-katalysatoren und ihre bereitung. |
| AU644352B2 (en) * | 1988-12-08 | 1993-12-09 | Damon Biotech Inc. | Expression induction method employing mutant dhfr gene |
| US5968502A (en) * | 1991-11-05 | 1999-10-19 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
| TW402639B (en) * | 1992-12-03 | 2000-08-21 | Transkaryotic Therapies Inc | Protein production and protein delivery |
| JP5648912B2 (ja) | 2008-02-27 | 2015-01-07 | 国立大学法人北海道大学 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
| ES2552325T3 (es) | 2008-12-22 | 2015-11-27 | National University Corporation Hokkaido University | Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo |
| WO2011019619A1 (en) | 2009-08-11 | 2011-02-17 | Genentech, Inc. | Production of proteins in glutamine-free cell culture media |
| RU2500817C1 (ru) * | 2012-07-19 | 2013-12-10 | Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рВК415, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 1F8 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ И ВЫДЕЛЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА |
| EP4332231A3 (de) | 2014-04-29 | 2024-05-29 | Novartis AG | Neuartige wirbeltierzellen und verfahren zur rekombinanten expression eines polypeptids von interesse |
| JP7264353B2 (ja) | 2018-05-24 | 2023-04-25 | 国立大学法人北海道大学 | 新規ベクターおよびその利用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| JPS5879205A (ja) * | 1981-11-06 | 1983-05-13 | Canon Inc | 色分解用フイルタ−の製造方法 |
| AR241654A1 (es) * | 1982-05-05 | 1992-10-30 | Genentech Inc | Procedimiento para producir activador de plasminogeno de tejido humano. |
| AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
| JPS61185427A (ja) * | 1985-02-13 | 1986-08-19 | Toyoda Gosei Co Ltd | 射出装置 |
| JPS6423654A (en) * | 1987-07-17 | 1989-01-26 | Fujitsu Ltd | Pre-emphasis circuit |
-
1984
- 1984-01-17 AU AU23534/84A patent/AU572108B2/en not_active Expired
- 1984-01-17 IE IE97/84A patent/IE56716B1/en not_active IP Right Cessation
- 1984-01-18 AT AT84300299T patent/ATE59410T1/de not_active IP Right Cessation
- 1984-01-18 DK DK198400211A patent/DK174081B1/da not_active IP Right Cessation
- 1984-01-18 EP EP84300299A patent/EP0117059B1/de not_active Expired - Lifetime
- 1984-01-18 ZA ZA84375A patent/ZA84375B/xx unknown
- 1984-01-18 DE DE8484300299T patent/DE3483839D1/de not_active Expired - Lifetime
- 1984-01-19 CA CA000445592A patent/CA1271149A/en not_active Expired - Lifetime
- 1984-01-19 ES ES529023A patent/ES8507610A1/es not_active Expired
- 1984-01-19 JP JP59007898A patent/JPS59183693A/ja active Pending
- 1984-01-19 KR KR1019840000220A patent/KR840007255A/ko not_active Ceased
-
1988
- 1988-08-04 MY MYPI88000886A patent/MY101958A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IE840097L (en) | 1984-07-19 |
| JPS59183693A (ja) | 1984-10-18 |
| AU572108B2 (en) | 1988-05-05 |
| EP0117059B1 (de) | 1990-12-27 |
| ES529023A0 (es) | 1985-10-01 |
| EP0117059A3 (en) | 1986-03-05 |
| DE3483839D1 (de) | 1991-02-07 |
| IE56716B1 (en) | 1991-11-20 |
| DK21184D0 (da) | 1984-01-18 |
| AU2353484A (en) | 1984-07-26 |
| MY101958A (en) | 1992-02-15 |
| DK174081B1 (da) | 2002-05-27 |
| CA1271149A (en) | 1990-07-03 |
| EP0117059A2 (de) | 1984-08-29 |
| KR840007255A (ko) | 1984-12-06 |
| DK21184A (da) | 1984-08-24 |
| ZA84375B (en) | 1985-02-27 |
| ES8507610A1 (es) | 1985-10-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE3483839D1 (de) | Verfahren zur herstellung von menschlichem tpa und expressionsvektoren dafuer. | |
| DE68928934D1 (de) | Verfahren zur herstellung von menschlicher dnase | |
| DE3479321D1 (en) | Biosynthetically produced human nerve growth factor, process for producing it, compositions containing it, dna sequence encoding it, vectors containing the sequence and host cells transformed thereby | |
| DK306386A (da) | Fibronectin og polypeptidsekvenser omfattende de collagenbindende og/eller fibrinbindende dele deraf, cdna-sekvenser, som koder herfor, vektorer indeholdende cdna-sekvenserne mikroorganismer transformeret dermed og fremgangsmaade til rensning af stoffer | |
| DE3855921D1 (de) | Verfahren und Desoxyribonukleinsäure zur Herstellung von Gewebefaktor-Protein | |
| ATE107362T1 (de) | Polypeptid-mutanten des menschlichen tnf und für diese mutanten codierende dns. | |
| DE3682891D1 (de) | Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen. | |
| ATE75775T1 (de) | Verfahren zur herstellung von biologisch aktivem plasminogenaktivator. | |
| DE3872049D1 (de) | Alkohol, fett und protein enthaltendes aufschaumbares produkt sowie verfahren zu seiner herstellung. | |
| HUT56884A (en) | Process for producing protein having urate oxidase activity, gene coding for it, expression vector, microorganisms and transformed cells | |
| ATE67242T1 (de) | Verfahren zur verbesserung der expression von eukaryontenprotein. | |
| ATE95836T1 (de) | Zum abbau von glycosaminoglycan faehige enzymzusammensetzung und verfahren zur herstellung dieser zusammensetzung. | |
| ATE194624T1 (de) | Mutanten von menschlichem antithrombin iii | |
| SE8702562D0 (sv) | Novel fibrinolytic enzymes | |
| DE68907166D1 (de) | Verfahren zur herstellung von menschlichem lysozym. | |
| ATE102994T1 (de) | Verfahren zur herstellung von proteinen. | |
| DE3783299D1 (de) | Herstellung von mikroorganismen mit eiskristallbildungsaktivitaet. | |
| DE68928634D1 (de) | Verfahren zur Herstellung von aktivierten Killer-Monozyten und Verfahren zur Überprüfung ihrer tumoriziden Aktivität | |
| DE3766667D1 (de) | Verfahren zur herstellung von homo- und copolymeren des vinylchlorids in emulsion oder mikrosuspension. | |
| ATE48531T1 (de) | Verfahren zum sterilisieren von rekombinantem mikroorganismus. | |
| DE58902277D1 (de) | Verfahren zur herstellung von gewuerzzubereitungen. | |
| FI894657A0 (fi) | Menetelmä ihmiskudostyyppisen plasminogeeniaktivaattorin tuottamiseksi | |
| DE3860417D1 (de) | 1-fluor-1-halogen dioxa-3,6-bizyklo(4.1.0)heptan, verfahren zur herstellung und verwendung. | |
| DE58909205D1 (de) | Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten. | |
| JPS5794290A (en) | Method for producing interferon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EELA | Cancelled due to lapse of time |