ES8507610A1 - Produccion de activador de plasminogeno de tejido humano, utilizando vectores que codifican proteina de dihidrofolato- reductasa. - Google Patents

Produccion de activador de plasminogeno de tejido humano, utilizando vectores que codifican proteina de dihidrofolato- reductasa.

Info

Publication number
ES8507610A1
ES8507610A1 ES529023A ES529023A ES8507610A1 ES 8507610 A1 ES8507610 A1 ES 8507610A1 ES 529023 A ES529023 A ES 529023A ES 529023 A ES529023 A ES 529023A ES 8507610 A1 ES8507610 A1 ES 8507610A1
Authority
ES
Spain
Prior art keywords
protein
sequence
exportation
methods
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES529023A
Other languages
English (en)
Other versions
ES529023A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ES529023A0 publication Critical patent/ES529023A0/es
Publication of ES8507610A1 publication Critical patent/ES8507610A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Electrotherapy Devices (AREA)

Abstract

PROCEDIMIENTO PARA LA PRODUCCION DE PLASMINOGENO DE TEJIDO HUMANO EN UN CULTIVO DE CELULAS HOSPEDANTES TRANSFORMANTES, UTILIZANDO VECTORES QUE CODIFICAN PROTEINA DE DIHIDROFOLATO-REDUCTASA.CONSISTE EN AMPLIFICAR LA SECUENCIA DE ADN QUE CODIFICA TPA, EN UN CULTIVO DE CELULAS HOSPEDANTES, PROPORCIONANDO UN VECTOR DE EXPRESION, Y HACIENDO CRECER LAS CELULAS EN PRESENCIA DE UNA CONCENTRACION AMPLIFICADORA EFICAZ DE MTX. EL VECTOR DE EXPRESION COMPRENDE UNA PRIMERA SECUENCIA DE ACIDO DESOXIRRIBONUCLEICO (ADN) QUE CODIFICA UNA PROTEINA DE DIHIDROFOLATO-REDUCTASA (DHFR) Y UNA SEGUNDA SECUENCIA DE ADN QUE CODIFICA ACTIVADOR DE PLASMINOGENO DE TEJIDO HUMANO (TPA).
ES529023A 1983-01-19 1984-01-19 Produccion de activador de plasminogeno de tejido humano, utilizando vectores que codifican proteina de dihidrofolato- reductasa. Expired ES8507610A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45915383A 1983-01-19 1983-01-19

Publications (2)

Publication Number Publication Date
ES529023A0 ES529023A0 (es) 1985-10-01
ES8507610A1 true ES8507610A1 (es) 1985-10-01

Family

ID=23823628

Family Applications (1)

Application Number Title Priority Date Filing Date
ES529023A Expired ES8507610A1 (es) 1983-01-19 1984-01-19 Produccion de activador de plasminogeno de tejido humano, utilizando vectores que codifican proteina de dihidrofolato- reductasa.

Country Status (12)

Country Link
EP (1) EP0117059B1 (es)
JP (1) JPS59183693A (es)
KR (1) KR840007255A (es)
AT (1) ATE59410T1 (es)
AU (1) AU572108B2 (es)
CA (1) CA1271149A (es)
DE (1) DE3483839D1 (es)
DK (1) DK174081B1 (es)
ES (1) ES8507610A1 (es)
IE (1) IE56716B1 (es)
MY (1) MY101958A (es)
ZA (1) ZA84375B (es)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713339A (en) * 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
AU2353384A (en) * 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
KR850004274A (ko) * 1983-12-13 1985-07-11 원본미기재 에리트로포이에틴의 제조방법
GB8415186D0 (en) * 1984-06-14 1984-07-18 Ciba Geigy Ag Polypeptides
EP0174835A1 (en) * 1984-09-11 1986-03-19 The Upjohn Company Human tissue plasminogen activator and recombinant DNA compounds
DK175327B1 (da) * 1984-10-01 2004-08-23 Integrated Genetics Inc Replicerbar ekspressionsvektor og rekombinant DNA-molekyle, som indkoder aktiv human livmodervævsplasminogen-aktivator, pattedyrscelle transformeret med vektoren og fremgangsmåde til fremstilling af aktiv human vævsplasminogen-aktivator (TPA)
GR860875B (en) * 1985-04-04 1986-07-29 Beecham Group Plc Novel compounds
US5672502A (en) * 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
NZ217331A (en) * 1985-08-26 1989-05-29 Lilly Co Eli Tissue plasminogen activator derivatives and genetically engineered product
GB8528321D0 (en) * 1985-11-18 1985-12-24 Ciba Geigy Ag Modified fibrinolytic agents
JPH0714341B2 (ja) * 1985-11-20 1995-02-22 鐘淵化学工業株式会社 蛋白質の高生産株の取得方法
US5132214A (en) * 1986-04-09 1992-07-21 Monsanto Company Large scale production of plasminogen activator from normal human colon cells
US4751084A (en) * 1986-02-26 1988-06-14 Monsanto Company Tissue plasminogen activator from normal human colon cells
US4927630A (en) * 1986-02-26 1990-05-22 Monsanto Company Tissue plasminogen activator from normal human colon cells
US4851517A (en) * 1986-02-26 1989-07-25 Monsanto Company Tissue plasminogen activator oligosaccharide from normal human colon cells
CA1297434C (en) * 1986-04-14 1992-03-17 Kenji Murakami Method of producing peptides, recombinant plasmid for use in the same and animal cells transformed with the same
JP2585532B2 (ja) * 1986-05-10 1997-02-26 三井東圧化学株式会社 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法
JPS62265992A (ja) * 1986-05-12 1987-11-18 Ajinomoto Co Inc ヒト細胞を用いる有用物質の製造方法
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
JPS63105675A (ja) * 1986-10-23 1988-05-10 Green Cross Corp:The ヒトウロキナ−ゼの製造方法
PT86162B (pt) * 1986-11-21 1990-11-20 Smithkline Beecham Corp Expressao de proteina recombinante
ZA879286B (en) 1986-12-16 1988-10-26 Smith Kline Rit New plasminogen activators
EP0281246A3 (en) * 1987-02-04 1989-07-12 Invitron Corporation Method to enhance amplification and expression of foreign genes
NZ219516A (en) * 1987-02-10 1991-02-26 Wellcome Found Fusion proteins of hbcag (hepatitis b virus core antigen)
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
CA1341345C (en) * 1987-05-08 2002-03-05 Hanne Ranch Johansen Expression of foreign genes in drosophila cells
US5681713A (en) * 1987-05-08 1997-10-28 Smithkline Beecham Corporation Expression of heterologous proteins in Drosophila cells
US4873316A (en) * 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5017478A (en) * 1987-07-16 1991-05-21 Berlex Laboratories, Inc. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using
DE3880113T2 (de) * 1987-12-21 1993-07-15 Unilever Nv Nickel-kieselerde-katalysatoren und ihre bereitung.
AU644352B2 (en) * 1988-12-08 1993-12-09 Damon Biotech Inc. Expression induction method employing mutant dhfr gene
US5968502A (en) * 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
JP5648912B2 (ja) 2008-02-27 2015-01-07 国立大学法人北海道大学 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
ES2552325T3 (es) 2008-12-22 2015-11-27 National University Corporation Hokkaido University Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo
WO2011019619A1 (en) 2009-08-11 2011-02-17 Genentech, Inc. Production of proteins in glutamine-free cell culture media
RU2500817C1 (ru) * 2012-07-19 2013-12-10 Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рВК415, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 1F8 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ И ВЫДЕЛЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕНА
EP4332231A3 (en) 2014-04-29 2024-05-29 Novartis AG Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest
JP7264353B2 (ja) 2018-05-24 2023-04-25 国立大学法人北海道大学 新規ベクターおよびその利用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
JPS5879205A (ja) * 1981-11-06 1983-05-13 Canon Inc 色分解用フイルタ−の製造方法
AR241654A1 (es) * 1982-05-05 1992-10-30 Genentech Inc Procedimiento para producir activador de plasminogeno de tejido humano.
AU2353384A (en) * 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
JPS61185427A (ja) * 1985-02-13 1986-08-19 Toyoda Gosei Co Ltd 射出装置
JPS6423654A (en) * 1987-07-17 1989-01-26 Fujitsu Ltd Pre-emphasis circuit

Also Published As

Publication number Publication date
IE840097L (en) 1984-07-19
JPS59183693A (ja) 1984-10-18
AU572108B2 (en) 1988-05-05
EP0117059B1 (en) 1990-12-27
ES529023A0 (es) 1985-10-01
EP0117059A3 (en) 1986-03-05
DE3483839D1 (de) 1991-02-07
IE56716B1 (en) 1991-11-20
DK21184D0 (da) 1984-01-18
AU2353484A (en) 1984-07-26
MY101958A (en) 1992-02-15
DK174081B1 (da) 2002-05-27
CA1271149A (en) 1990-07-03
EP0117059A2 (en) 1984-08-29
KR840007255A (ko) 1984-12-06
DK21184A (da) 1984-08-24
ZA84375B (en) 1985-02-27
ATE59410T1 (de) 1991-01-15

Similar Documents

Publication Publication Date Title
ES8105386A1 (es) Metodo para construir un vehiculo de reproduccion o clona- cion
ES8602121A1 (es) Procedimiento para producir un vehiculo de expresion para la formacion de uroquinasa humana
ES8507179A1 (es) Un metodo de conseguir una deseada proteina madura en una celula hospedante de vertebrado.
ES529023A0 (es) Produccion de activador de plasminogeno de tejido humano, utilizando vectores que codifican proteina de dihidrofolato- reductasa.
ES8706726A1 (es) Un metodo de purificar activador de plasminogeno de tejido uterino
ES501196A0 (es) Metodo para sintetizar dentro de un hospedador bacteriano,y secretar a traves de la membrana del hospedador,una proteinao polipeptido
ES8501629A1 (es) Procedimiento para producir t-p-a humano activador de plasminogeno.
ES8506801A1 (es) Un metodo para producir un gen que contiene una muteina sintetica de una proteina biologicamente activa
ES8600783A1 (es) Procedimiento para la preparacion de la hormona polipeptidica factor de crecimiento de nervios de humano.
ES8603957A1 (es) Procedimiento para obtener una proteina heterologa para levadura
ES8700868A1 (es) Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.
DK133388A (da) Human mangan-superoxiddismutase (hmn-sod)
ES8703490A1 (es) Un procedimiento para producir un polipeptido que tiene o contiene un polipeptido de factor de necrosis de tumor humano
ES8603955A1 (es) Preparacion de igf y egf de humano por tecnologia de adn recombinante
ATE172493T1 (de) Rekombinante dns-mittel und verfahren
ES8207514A1 (es) Un procedimiento para la preparacion de interferon
DK431988D0 (da) Protein og dna til kodning deraf
ES2073735T3 (es) Nuevos analogos de la cadena b del factor de crecimiento derivado de plaquetas y metodo para su produccion homogenea.
MX9302917A (es) Derivados de proteinas c humanas, procedimiento para su preparacion y formulacion famaceutica que los contiene.
ES8505405A1 (es) Un procedimiento para producir un activador de plasminogeno purificado.
ATE107960T1 (de) Varianten von menschlichem gewebeplasminogenaktivator sowie verfahren zu deren herstellung.
DK662288A (da) Vasculaere antikoagulerende proteiner, dna som koder for disse og fremgangsmaade til fremstilling deraf
ES2007425A6 (es) Un metodo para la produccion de una proteina con actividad inhibidora del crecimiento de tumores.
ATE102994T1 (de) Verfahren zur herstellung von proteinen.
ATE254171T1 (de) Gentherapeutisches verfahren unter verwendung von dna-vektoren ohne selektionsmarker-gen

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050614