ATE81674T1 - Gereinigtes rekombinantes interleukin-2 und verfahren zu dessen rueckgewinnung und reinigung. - Google Patents

Gereinigtes rekombinantes interleukin-2 und verfahren zu dessen rueckgewinnung und reinigung.

Info

Publication number: ATE81674T1
Authority: AT; Austria
Prior art keywords: purification; recovery; methods; purified recombinant; cellular material
Prior art date: 1984-03-28

Application number

AT85103649T

Other languages

English (en)

Inventor

Kirston Edward Koths

James William Thomson

Michael Kunitani

Kenneth Wilson

Wolfgang Helmut Hanisch

Original Assignee

Cetus Oncology Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-03-28

Filing date

1985-03-27

Publication date

1992-11-15

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24378044&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE81674(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1985-03-27 Application filed by Cetus Oncology Corp filed Critical Cetus Oncology Corp

1992-11-15 Application granted granted Critical

1992-11-15 Publication of ATE81674T1 publication Critical patent/ATE81674T1/de

Links

108010002350 Interleukin-2 Proteins 0.000 title abstract 6
238000000034 method Methods 0.000 title abstract 2
102000000588 Interleukin-2 Human genes 0.000 title 1
238000000746 purification Methods 0.000 title 1
238000011084 recovery Methods 0.000 title 1
MSXVEPNJUHWQHW-UHFFFAOYSA-N 2-methylbutan-2-ol Chemical compound CCC(C)(C)O MSXVEPNJUHWQHW-UHFFFAOYSA-N 0.000 abstract 3
BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 abstract 3
230000001413 cellular effect Effects 0.000 abstract 2
238000000605 extraction Methods 0.000 abstract 2
239000000463 material Substances 0.000 abstract 2
244000005700 microbiome Species 0.000 abstract 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
239000007864 aqueous solution Substances 0.000 abstract 1
239000004202 carbamide Substances 0.000 abstract 1
210000000170 cell membrane Anatomy 0.000 abstract 1
230000003196 chaotropic effect Effects 0.000 abstract 1
239000003638 chemical reducing agent Substances 0.000 abstract 1
239000003795 chemical substances by application Substances 0.000 abstract 1
239000000284 extract Substances 0.000 abstract 1
238000001641 gel filtration chromatography Methods 0.000 abstract 1
230000000813 microbial effect Effects 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
230000001590 oxidative effect Effects 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
238000004007 reversed phase HPLC Methods 0.000 abstract 1
239000007790 solid phase Substances 0.000 abstract 1
230000003381 solubilizing effect Effects 0.000 abstract 1
239000000243 solution Substances 0.000 abstract 1
239000002904 solvent Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Biochemistry (AREA)
Zoology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Engineering & Computer Science (AREA)
General Chemical & Material Sciences (AREA)
General Engineering & Computer Science (AREA)
Analytical Chemistry (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Toxicology (AREA)
Wood Science & Technology (AREA)
Gastroenterology & Hepatology (AREA)
Biomedical Technology (AREA)
Animal Behavior & Ethology (AREA)
Physics & Mathematics (AREA)
Veterinary Medicine (AREA)
Public Health (AREA)
Plant Pathology (AREA)
Pharmacology & Pharmacy (AREA)
Microbiology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

AT85103649T 1984-03-28 1985-03-27 Gereinigtes rekombinantes interleukin-2 und verfahren zu dessen rueckgewinnung und reinigung. ATE81674T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US06/594,223 US4569790A (en)	1984-03-28	1984-03-28	Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
EP85103649A EP0156373B1 (de)	1984-03-28	1985-03-27	Gereinigtes rekombinantes Interleukin-2 und Verfahren zu dessen Rückgewinnung und Reinigung

Publications (1)

Publication Number	Publication Date
ATE81674T1 true ATE81674T1 (de)	1992-11-15

Family

ID=24378044

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT85103649T ATE81674T1 (de)	1984-03-28	1985-03-27	Gereinigtes rekombinantes interleukin-2 und verfahren zu dessen rueckgewinnung und reinigung.

Country Status (20)

Country	Link
US (3)	US4569790A (de)
EP (2)	EP0156373B1 (de)
JP (2)	JP2599903B2 (de)
KR (1)	KR850007083A (de)
AT (1)	ATE81674T1 (de)
AU (1)	AU592751B2 (de)
CA (1)	CA1341083C (de)
DE (2)	DE3588189T2 (de)
DK (1)	DK166324C (de)
ES (1)	ES8609362A1 (de)
FI (1)	FI94960C (de)
GR (1)	GR850741B (de)
IE (1)	IE59100B1 (de)
IL (1)	IL74733A (de)
IN (1)	IN162903B (de)
NO (1)	NO851235L (de)
NZ (1)	NZ211569A (de)
PH (1)	PH21012A (de)
PT (1)	PT80172B (de)
ZA (1)	ZA852347B (de)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4925919A (en) *	1984-04-25	1990-05-15	Roland Mertelsmann	Purified interleukin 2
US4992271A (en) *	1982-09-23	1991-02-12	Cetus Corporation	Formulation for lipophilic IL-2 proteins
US4853332A (en) *	1982-10-19	1989-08-01	Cetus Corporation	Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of biologically active proteins
JPS60115528A (ja) *	1983-11-28	1985-06-22	Takeda Chem Ind Ltd	ヒトインタ―ロイキン―２蛋白質を含有する抗腫瘍用または免疫機能低下疾患治療用組成物
DK174501B1 (da) *	1983-12-23	2003-04-28	Hoffmann La Roche	Fremgangsmåde til fremstilling af interleukin-2
US4656132A (en) *	1984-03-28	1987-04-07	Cetus Corporation	Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
US4530787A (en) *	1984-03-28	1985-07-23	Cetus Corporation	Controlled oxidation of microbially produced cysteine-containing proteins
US4569790A (en) *	1984-03-28	1986-02-11	Cetus Corporation	Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
DE3583880D1 (de) *	1984-04-09	1991-10-02	Takeda Chemical Industries Ltd	Stabile interleukin-2-zusammensetzung.
US4908434A (en) *	1984-04-25	1990-03-13	Sloan-Kettering Institute For Cancer Research	Process for preparing purified interleukin-2
US4908433A (en) *	1984-04-25	1990-03-13	Sloan-Kettering Institute For Cancer Research	Uses of interleukin-2
US4572798A (en) *	1984-12-06	1986-02-25	Cetus Corporation	Method for promoting disulfide bond formation in recombinant proteins
DE3500681A1 (de) *	1985-01-11	1986-07-17	Hoechst Ag, 6230 Frankfurt	Verfahren zur isolierung und reinigung von lymphokinen
JPH0646957B2 (ja) *	1985-03-11	1994-06-22	武田薬品工業株式会社	インタ−ロイキン−２の製造方法
US5523215A (en) *	1985-03-28	1996-06-04	Chiron Corporation	Enhanced purification and expression of insoluble recombinant proteins
US4748234A (en) *	1985-06-26	1988-05-31	Cetus Corporation	Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US5248769A (en) *	1985-06-26	1993-09-28	Cetus Oncology Corporation	Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4788144A (en) *	1985-06-28	1988-11-29	International Minerals & Chemical Corp.	Fermentation process for the high level production of swine growth
US4762784A (en) *	1985-07-15	1988-08-09	International Minerals & Chemical Corp.	Fermemtation process for the high level production of bovine growth hormone
US4690915A (en) *	1985-08-08	1987-09-01	The United States Of America As Represented By The Department Of Health And Human Services	Adoptive immunotherapy as a treatment modality in humans
CA1297003C (en) *	1985-09-20	1992-03-10	Jack H. Nunberg	Composition and method for treating animals
US4766205A (en) *	1985-11-13	1988-08-23	Beatrice Companies, Inc.	Method for isolation of recombinant polypeptides in biologically active forms
US4933433A (en) *	1986-01-31	1990-06-12	E. I. Du Pont De Nemours And Company	Recombinant interleukin-2 composition and process for making it
US5425940A (en) *	1986-04-09	1995-06-20	Cetus Oncology Corporation	Combination therapy using interleukin-2 and tumor necrosis factor
US4931544A (en) *	1986-09-04	1990-06-05	Cetus Corporation	Succinylated interleukin-2 for pharmaceutical compositions
US4683293A (en) *	1986-10-20	1987-07-28	Phillips Petroleum Company	Purification of pichia produced lipophilic proteins
US4894330A (en) *	1986-12-23	1990-01-16	Cetus Corporation	Purification of recombinant beta-interferon incorporating RP-HPLC
US4879374A (en) *	1987-03-13	1989-11-07	Immunex Corporation	Bovine interleukin-1β DNA sequence
US5108911A (en) *	1987-03-13	1992-04-28	Immunex Corporation	Process for preparing bovine interleukin-Iβ
CA1339757C (en) *	1987-04-16	1998-03-17	Robert F. Halenbeck	Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) *	1987-04-16	1990-05-29	Cetus Corporation	Production of purified, biologically active, bacterially produced recombinant human CSF-1
JPS6463395A (en)	1987-05-04	1989-03-09	Oncogen	Oncostatin m and novel composition having antitumor activity
US4931543A (en) *	1987-05-11	1990-06-05	Cetus Corporation	Process for recovering microbially produced interleukin-2
ATE156491T1 (de) *	1987-05-11	1997-08-15	Chiron Corp	Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
US5162507A (en) *	1987-05-11	1992-11-10	Cetus Corporation	Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4801691A (en) *	1987-05-15	1989-01-31	International Minerals & Chemical Corp.	Method for removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilized protein solutions
KR890701759A (ko) *	1987-07-29	1989-12-21	스테이너 브이. 칸스태드	에스케리키아 콜라이내에서 발현된 사람 인터루킨-4의 정제방법
JP2669859B2 (ja) *	1987-08-04	1997-10-29	協和醗酵工業株式会社	タンパク質の再活性化法
EP0337243A1 (de) *	1988-04-14	1989-10-18	F. Hoffmann-La Roche Ag	Verfahren zur Reinigung von rekombinantem Interleukin-2
FR2635527B1 (fr) *	1988-07-28	1992-06-12	Roussel Uclaf	Il2 humaine recombinante non glycosylee sous forme reduite, son procede d'obtention et son application comme medicament
US4923967A (en) *	1988-09-26	1990-05-08	Eli Lilly And Company	Purification and refolding of recombinant proteins
US5830452A (en) *	1990-11-20	1998-11-03	Chiron Corporation	Method for enhancing the anti-tumor therapeutic index of interleukin-2
ATE159260T1 (de) *	1990-11-23	1997-11-15	Roussel Uclaf	Verfahren zur herstellung eines proteins gehörend zu der klasse der cytokine, das mindestens eine intramolekulare disulfidbrücke enthält, durch oxidation bei einem ph-wert kleiner als 5.0 des entsprechenden rekombinanten reduzierten proteins
US5939524A (en) *	1991-12-09	1999-08-17	The Scripps Research Institute	Platelet GPIII P1A1 and P1A2 epitopes, their preparation and use
AU648214B2 (en) *	1991-12-31	1994-04-14	Lucky Limited	Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
FR2686899B1 (fr) *	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6063764A (en) *	1992-06-01	2000-05-16	Washington University & Chiron Corp.	Method for using lipoprotein associated coagulation inhibitor to treat sepsis
US20030171292A1 (en) *	1992-06-01	2003-09-11	Creasey Abla A.	Method for using lipoprotein associated coagulation inhibitor to treat sepsis
AU687763B2 (en) *	1992-10-20	1998-03-05	Central Laboratory Of The Netherlands Red Cross Blood Transfusion Service	Interleukin-6 receptor antagonists
CN1051092C (zh) *	1993-05-21	2000-04-05	沈阳军区后勤部军事医学研究所	一种重组大肠杆菌培养液中分离和纯化重组人白细胞介素2的方法
US5632983A (en)	1994-11-17	1997-05-27	University Of South Florida	Method for treating secondary immunodeficiency
US5912327A (en) *	1996-09-30	1999-06-15	Human Genome Sciences, Inc.	Method of purifying chemokines from inclusion bodies
US6632425B1 (en)	1997-03-20	2003-10-14	Human Genome Sciences, Inc.	Chemokine compositions
EP1935431A3 (de)	2000-05-15	2008-08-13	Health Research, Inc.	Krebsbehandlungen unter Verwendung einer Kombination aus einem HER2-Antikörper und Interleukin-2
JP4303969B2 (ja)	2001-03-29	2009-07-29	シェーリングコーポレイション	Ｃｃｒ５アンタゴニストとして有用なアリールオキシム−ピペラジン
US7674769B2 (en) *	2001-10-15	2010-03-09	Novartis Vaccines And Diagnostics, Inc.	Treatment of severe pneumonia by administration of tissue factor pathway inhibitor (TFPI)
WO2003099320A1 (en)	2002-05-24	2003-12-04	Zensun (Shanghai) Sci-Tech.Ltd	Neuregulin based methods and compositions for treating viral myocarditis and dilated cardiomyopathy
BRPI0408704A (pt)	2003-03-24	2006-03-07	Hoffmann La Roche	benzil-piridazinonas como inibidores de transcriptase reversa
JP4642025B2 (ja) *	2003-09-09	2011-03-02	ノバルティスバクシンズアンドダイアグノスティックス，インコーポレーテッド	治療的ポリペプチドのクロマトグラフィー分離
BRPI0415771A (pt)	2003-10-23	2006-12-26	Illumigen Biosciences Inc	detecção de mutações em um gene associado à resistência a infecção viral, oas1
ATE473016T1 (de)	2003-11-04	2010-07-15	Novartis Vaccines & Diagnostic	Verfahren zur behandlung von krebs mit expression des cd40-antigens
CN101426520A (zh) *	2004-03-17	2009-05-06	诺华疫苗和诊断公司	通过给予组织因子途径抑制剂（tfpi）治疗社区获得性重症肺炎
CU23297A1 (es)	2004-11-16	2008-07-24	Ct De Inmunologa A Molecular	Formulaciones inmunoterapã0/00uticas para la inducciã"n de autoanticuerpos bloqueadores de la uniã"n de interleucina-2 a su receptor. su uso en el tratamiento del cã ncer
DE102005005542A1 (de) *	2005-02-07	2006-08-10	Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville	Herstellung von Aldesleukin für die pharmazeutische Verwendung
WO2007045573A1 (en)	2005-10-19	2007-04-26	F. Hoffmann-La Roche Ag	Phenyl-acetamide nnrt inhibitors
RU2304586C1 (ru) *	2006-03-27	2007-08-20	Михаил Николаевич Смирнов	Препарат интерлейкина-2 и способ его получения
BRPI0715193A2 (pt)	2006-08-16	2013-10-01	Hoffmann La Roche	inibidores nço-nucleosÍdicos da transcriptase reversa
JP5415957B2 (ja)	2006-12-13	2014-02-12	エフ．ホフマン−ラロシュアーゲー	非ヌクレオシド逆転写酵素阻害剤としての２−（ピペリジン−４−イル）−４−フェノキシ−又はフェニルアミノ−ピリミジン誘導体
AU2008257771B2 (en)	2007-05-30	2015-02-19	F. Hoffmann-La Roche Ag	Non-nucleoside reverse transcriptase inhibitors
CN101903382B (zh)	2007-12-21	2012-11-28	弗·哈夫曼-拉罗切有限公司	杂环抗病毒化合物
BRPI0917961A2 (pt) *	2008-08-04	2017-04-11	Kai Bioenergy	cultivo, colheita e extração contínua de óleo de culturas fotossintéticas
HRP20210642T1 (hr)	2010-11-12	2021-05-28	Nektar Therapeutics	Konjugati il-2 ostatka i polimera
US9428567B2 (en)	2010-12-22	2016-08-30	The Board Of Trustees Of The Leland Stanford Junior University	Antagonists of interleukin-2 receptor
WO2013180650A1 (en) *	2012-05-31	2013-12-05	Agency For Science, Technology And Research	Methods for reducing levels of protein-contaminant complexes and aggregates in protein preparations by treatment with electropositive organic additives
CA2925421C (en)	2013-09-24	2023-08-29	Medicenna Therapeutics, Inc.	Interleukin-2 fusion proteins and uses thereof
WO2015164815A1 (en)	2014-04-24	2015-10-29	The Board Of Trustees Of The Leland Stanford Junior University	Superagonists, partial agonists and antagonists of interleukin-2
WO2016057651A1 (en)	2014-10-09	2016-04-14	Dana-Farber Cancer Institute, Inc.	Multiple-variable il-2 dose regimen for treating immune disorders
KR102861230B1 (ko)	2015-10-08	2025-09-17	넥타르 테라퓨틱스	Il-2r베타-선택적 효현제 및 지속 작용성 il-15 효현제의 조합
JP7433051B2 (ja)	2017-06-19	2024-02-19	メディシナセラピューティクスインコーポレイテッド	Ｉｌ－２スーパーアゴニスト、アゴニスト、およびそれらの融合体の使用および方法
CA3069321A1 (en)	2017-07-11	2019-01-17	The Scripps Research Institute	Incorporation of unnatural nucleotides and methods thereof
KR20250145137A (ko)	2017-08-03	2025-10-13	신톡스, 인크.	자가면역 질환의 치료를 위한 사이토카인 접합체
PH12020550661A1 (en)	2017-11-21	2021-04-19	Univ Leland Stanford Junior	Partial agonists of interleukin-2
JOP20200290A1 (ar)	2018-05-21	2020-11-15	Nektar Therapeutics	تركيبة RUR20kD-IL-2 الانتقائية لمحفز TREG وتركيبات ذات صلة
CA3103603A1 (en) *	2018-06-13	2019-12-19	Akron Biotechnology, LLC.	Method to prepare therapeutically active aldesleukin highly stable in liquid pharmaceutical compositions
SG11202107354WA (en)	2019-02-06	2021-08-30	Synthorx Inc	Il-2 conjugates and methods of use thereof
CN114245802B (zh)	2019-06-20	2024-10-15	石药集团巨石生物制药有限公司	修饰的il-2蛋白、peg偶联物及其用途
EP4090674A4 (de)	2020-01-14	2024-01-24	Synthekine, Inc.	Vorgespannte il2-muteins-verfahren und -zusammensetzungen
CN111676261A (zh) *	2020-06-24	2020-09-18	宁波博睿瀚达生物科技有限公司	一种高纯度重组白介素-2的制备工艺
CN111793124A (zh) *	2020-06-24	2020-10-20	宁波博睿瀚达生物科技有限公司	一种去除重组白介素-2内毒素的方法
WO2023114833A1 (en)	2021-12-14	2023-06-22	Eli Lilly And Company	Dosing regimens for selective treg stimulator rur20kd-il-2 and related compositions

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3897309A (en) *	1974-02-15	1975-07-29	Merck & Co Inc	Process for removing pyrogenic material from aqueous solutions
JPS6030291B2 (ja) *	1978-03-20	1985-07-16	森永乳業株式会社	人顆粒球の分化増殖を促進するｈｇｉ糖蛋白質、ｈｇｉ糖蛋白質の製造法及びｈｇｉ糖蛋白質を含有する白血球減少症治療剤
IN150740B (de) *	1978-11-24	1982-12-04	Hoffmann La Roche
JPS55148098A (en) *	1979-05-10	1980-11-18	Toray Ind Inc	Concentration and purification of interferon
CH648331A5 (de) *	1979-07-31	1985-03-15	Hoffmann La Roche	Homogenes fibroblasten-interferon und dessen herstellung.
US4390623A (en) *	1980-10-02	1983-06-28	Hooper Trading Company	Serum-free and mitogen-free T-cell growth factor and process for making same
US4315852A (en) *	1980-11-26	1982-02-16	Schering Corporation	Extraction of interferon from bacteria
US4464355A (en) *	1981-03-26	1984-08-07	Hooper Trading Co.	Serum-free and mitogen-free T-cell growth factor and process for making same
US4448879A (en) *	1981-03-26	1984-05-15	Hooper Trading Company	High yield process for in vitro production of serum-free and mitogen-free interleukin-2
US4401756A (en) *	1981-04-14	1983-08-30	Immunex Corporation	Process for preparing human interleukin 2
JPS58116498A (ja) *	1981-12-28	1983-07-11	Takeda Chem Ind Ltd	Ｉｌ‐２をコードする新規伝令ｒｎａの製造法
US4450103A (en) *	1982-03-01	1984-05-22	Cetus Corporation	Process for recovering human IFN-β from a transformed microorganism
CA1341562C (en) *	1982-03-31	2007-11-27	Tadatsugu Taniguchi	Gene coded for interleukin-2 polypeptide, recombinant dna carrying the said gene, a living cell line possessing the recombinant dna, and method for producing interleukin-2 using the said cell
DE3377363D1 (en) *	1982-03-31	1988-08-18	Ajinomoto Kk	Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells
US4925919A (en) *	1984-04-25	1990-05-15	Roland Mertelsmann	Purified interleukin 2
US4778879A (en) *	1982-04-20	1988-10-18	Sloan-Kettering Institute For Cancer Research	Highly purified human interleukin 2 and method
EP0092163B1 (de) *	1982-04-20	1988-09-28	Sloan-Kettering Institute For Cancer Research	Reinigung von Interleukin-2
JPS58198293A (ja) *	1982-05-12	1983-11-18	Shionogi & Co Ltd	インタ−ロイキン等免疫調節因子の製造方法
US4490289A (en) *	1982-09-16	1984-12-25	Hoffmann-La Roche Inc.	Homogeneous human interleukin 2
US4462940A (en) *	1982-09-23	1984-07-31	Cetus Corporation	Process for the recovery of human β-interferon-like polypeptides
US4992271A (en) *	1982-09-23	1991-02-12	Cetus Corporation	Formulation for lipophilic IL-2 proteins
DE3242851A1 (de) *	1982-11-19	1984-05-24	Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig	Verfahren zur reinigung von menschlichen lymphokinen
JPS59116231A (ja) *	1982-12-13	1984-07-05	スロ−ン−ケツタリング・インステイテユ−ト・フオ−・キヤンサ−・リサ−チ	抗−インタ−ロイキン−２単クロ−ン抗体
US4512922A (en) *	1982-12-22	1985-04-23	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
AU579089B2 (en) *	1983-02-08	1988-11-17	Biogen, Inc.	Human interleukin-2-like polypeptides
ZA842025B (en) *	1983-03-21	1984-11-28	Hoffmann La Roche	Interleuken-2
GB8308483D0 (en) *	1983-03-28	1983-05-05	Health Lab Service Board	Secretion of gene products
US4582799A (en) *	1983-04-15	1986-04-15	Damon Biotech, Inc.	Process for recovering nonsecreted substances produced by cells
US4518584A (en) *	1983-04-15	1985-05-21	Cetus Corporation	Human recombinant interleukin-2 muteins
NZ208309A (en) *	1983-06-01	1988-04-29	Hoffmann La Roche	Recombinant leukocyte, fibroblast, or immune interferons (rifn-#a#, b or #g#) with one cys residue replaced
WO1985000606A1 (fr) *	1983-07-19	1985-02-14	Takeda Chemical Industries, Ltd.	Il-2 humaine et son procede de preparation
US4464295A (en) *	1983-08-17	1984-08-07	The United States Of America As Represented By The Secretary Of Agriculture	Simple and rapid method for extraction of proteins from bacteria
US4476049A (en) *	1983-09-20	1984-10-09	Hoffmann-La Roche Inc.	Method for the extraction of immune interferon
GB8327880D0 (en) *	1983-10-18	1983-11-16	Ajinomoto Kk	Saccharomyces cerevisiae
US4675383A (en) *	1983-11-15	1987-06-23	The Salk Institute For Biological Studies	Purification of T-cell growth factor
JPS60115528A (ja) *	1983-11-28	1985-06-22	Takeda Chem Ind Ltd	ヒトインタ―ロイキン―２蛋白質を含有する抗腫瘍用または免疫機能低下疾患治療用組成物
DK174501B1 (da) *	1983-12-23	2003-04-28	Hoffmann La Roche	Fremgangsmåde til fremstilling af interleukin-2
JPS61500790A (ja) *	1984-03-28	1986-04-24	シタス　コ−ポレイシヨン	微生物的に製造されたインターロイキン―２を含有する組成物
US4569790A (en) *	1984-03-28	1986-02-11	Cetus Corporation	Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4530787A (en) *	1984-03-28	1985-07-23	Cetus Corporation	Controlled oxidation of microbially produced cysteine-containing proteins
EP0158198A1 (de) *	1984-03-29	1985-10-16	Takeda Chemical Industries, Ltd.	DNA und ihre Verwendung
DE3583880D1 (de) *	1984-04-09	1991-10-02	Takeda Chemical Industries Ltd	Stabile interleukin-2-zusammensetzung.
US4572798A (en) *	1984-12-06	1986-02-25	Cetus Corporation	Method for promoting disulfide bond formation in recombinant proteins
US4782139A (en) *	1985-10-28	1988-11-01	Eli Lilly And Company	Selective chemical removal of a protein amino-terminal residue
US4787658A (en) *	1986-07-29	1988-11-29	Harris Jr John	Unibody bumper system
US5156968A (en) *	1988-06-24	1992-10-20	Genentech, Inc.	Purified yeast ubiquitin hydrolase

1984
- 1984-03-28 US US06/594,223 patent/US4569790A/en not_active Expired - Lifetime
1985
- 1985-02-26 CA CA000475151A patent/CA1341083C/en not_active Expired - Lifetime
- 1985-03-25 PH PH32035A patent/PH21012A/en unknown
- 1985-03-25 IN IN220/CAL/85A patent/IN162903B/en unknown
- 1985-03-26 DK DK135985A patent/DK166324C/da not_active IP Right Cessation
- 1985-03-26 GR GR850741A patent/GR850741B/el unknown
- 1985-03-26 NZ NZ211569A patent/NZ211569A/en unknown
- 1985-03-27 NO NO851235A patent/NO851235L/no unknown
- 1985-03-27 FI FI851231A patent/FI94960C/fi not_active IP Right Cessation
- 1985-03-27 PT PT80172A patent/PT80172B/pt unknown
- 1985-03-27 EP EP85103649A patent/EP0156373B1/de not_active Expired - Lifetime
- 1985-03-27 DE DE3588189T patent/DE3588189T2/de not_active Expired - Lifetime
- 1985-03-27 EP EP91113235A patent/EP0470586B1/de not_active Expired - Lifetime
- 1985-03-27 ES ES541608A patent/ES8609362A1/es not_active Expired
- 1985-03-27 DE DE8585103649T patent/DE3586762T2/de not_active Expired - Lifetime
- 1985-03-27 IL IL74733A patent/IL74733A/xx not_active IP Right Cessation
- 1985-03-27 AT AT85103649T patent/ATE81674T1/de not_active IP Right Cessation
- 1985-03-28 IE IE79985A patent/IE59100B1/en not_active IP Right Cessation
- 1985-03-28 JP JP60062195A patent/JP2599903B2/ja not_active Expired - Lifetime
- 1985-03-28 ZA ZA852347A patent/ZA852347B/xx unknown
- 1985-03-28 AU AU40467/85A patent/AU592751B2/en not_active Expired
- 1985-03-28 KR KR1019850002078A patent/KR850007083A/ko not_active Ceased
1992
- 1992-05-06 US US07/879,753 patent/US5419899A/en not_active Expired - Lifetime
1995
- 1995-04-25 US US08/428,895 patent/US5614185A/en not_active Expired - Lifetime
1996
- 1996-05-15 JP JP8143485A patent/JP2648587B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
KR850007083A (ko)	1985-10-30
EP0470586B1 (de)	1998-07-15
AU4046785A (en)	1985-10-03
IN162903B (de)	1988-07-23
EP0156373A3 (en)	1987-09-02
CA1341083C (en)	2000-08-15
DK135985D0 (da)	1985-03-26
ES8609362A1 (es)	1986-09-01
PT80172A (en)	1985-04-01
FI94960B (fi)	1995-08-15
EP0470586A1 (de)	1992-02-12
DK135985A (da)	1985-09-29
IL74733A0 (en)	1985-06-30
AU592751B2 (en)	1990-01-25
HK1004474A1 (en)	1998-11-27
JP2648587B2 (ja)	1997-09-03
DK166324C (da)	1993-08-23
FI851231L (fi)	1985-09-29
DE3588189D1 (de)	1998-08-20
IE59100B1 (en)	1994-01-12
ES541608A0 (es)	1986-09-01
FI94960C (fi)	1995-11-27
DK166324B (da)	1993-04-05
GR850741B (de)	1985-06-26
PT80172B (pt)	1987-06-17
ZA852347B (en)	1986-11-26
DE3586762T2 (de)	1993-02-25
US4569790A (en)	1986-02-11
IE850799L (en)	1985-09-28
US5614185A (en)	1997-03-25
DE3588189T2 (de)	1998-11-19
IL74733A (en)	1990-11-05
EP0156373A2 (de)	1985-10-02
JPH08332089A (ja)	1996-12-17
JPS611393A (ja)	1986-01-07
JP2599903B2 (ja)	1997-04-16
NO851235L (no)	1985-09-30
PH21012A (en)	1987-06-23
NZ211569A (en)	1988-05-30
DE3586762D1 (de)	1992-11-26
FI851231A0 (fi)	1985-03-27
EP0156373B1 (de)	1992-10-21
US5419899A (en)	1995-05-30

Legal Events

Date	Code	Title
1993-05-15	UEP	Publication of translation of european patent specification
1999-01-15	EEIH	Change in the person of patent owner
2005-06-15	EELA	Cancelled due to lapse of time

Publication	Publication Date	Title
ATE81674T1 (de)	1992-11-15	Gereinigtes rekombinantes interleukin-2 und verfahren zu dessen rueckgewinnung und reinigung.
DK394786A (da)	1987-02-20	Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
DK426386A (da)	1987-03-07	Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
DK10189A (da)	1989-01-11	Fremgangsmaade til indvinding af renset, oxideret, renatureret interleukin-2 fra mikroorganismer
BR9810650A (pt)	2002-07-23	Vetor para expressão de proteìna heteróloga e métodos para extrair proteìna recombinante e para purificar insulina recombinante isolada
AU3053884A (en)	1985-01-17	Heterogeneous oligosaccharides
Koths et al.	1996	Purified, reduced recombinant interleukin-2 compositions
KR930013106A (ko)	1993-07-21	연어 성장 호르몬의 정제 방법