BRPI0507233B8 - processo para a produção de um polipeptídeo heterólogo em e. coli - Google Patents

processo para a produção de um polipeptídeo heterólogo em e. coli

Info

Publication number
BRPI0507233B8
BRPI0507233B8 BRPI0507233A BRPI0507233A BRPI0507233B8 BR PI0507233 B8 BRPI0507233 B8 BR PI0507233B8 BR PI0507233 A BRPI0507233 A BR PI0507233A BR PI0507233 A BRPI0507233 A BR PI0507233A BR PI0507233 B8 BRPI0507233 B8 BR PI0507233B8
Authority
BR
Brazil
Prior art keywords
coli
producing
heterologous polypeptide
polypeptide
cells
Prior art date
Application number
BRPI0507233A
Other languages
English (en)
Inventor
R Swartz James
Leung Woon-Lam Susan
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BRPI0507233A publication Critical patent/BRPI0507233A/pt
Publication of BRPI0507233B1 publication Critical patent/BRPI0507233B1/pt
Publication of BRPI0507233B8 publication Critical patent/BRPI0507233B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

processo de produção de polipeptídeo heterólogo em e. coli é descrito um processo de produção de polipeptídeo heterólogo a e. coli, em que as células de e. coli compreendem ácido nucléico codificador do polipeptídeo são cultivadas em meio de cultivo mediante alimentação ao meio de cultivo de organofosfato transportável, de tal forma que o ácido nucléico seja expresso. o polipeptídeo é recuperado das células em seguida.
BRPI0507233A 2004-03-11 2005-03-10 processo para a produção de um polipeptídeo heterólogo em e. coli BRPI0507233B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55267804P 2004-03-11 2004-03-11
US60/552,678 2004-03-11
PCT/US2005/007880 WO2005087802A2 (en) 2004-03-11 2005-03-10 Process for producing polypeptides

Publications (3)

Publication Number Publication Date
BRPI0507233A BRPI0507233A (pt) 2007-06-26
BRPI0507233B1 BRPI0507233B1 (pt) 2018-01-23
BRPI0507233B8 true BRPI0507233B8 (pt) 2021-05-25

Family

ID=34976293

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0507233A BRPI0507233B8 (pt) 2004-03-11 2005-03-10 processo para a produção de um polipeptídeo heterólogo em e. coli

Country Status (21)

Country Link
US (4) US7294483B2 (pt)
EP (1) EP1733037B1 (pt)
JP (1) JP5014116B2 (pt)
KR (1) KR101210083B1 (pt)
CN (1) CN1954074B (pt)
AU (1) AU2005222397A1 (pt)
BR (1) BRPI0507233B8 (pt)
CA (2) CA2558911C (pt)
DK (1) DK1733037T3 (pt)
ES (1) ES2527291T3 (pt)
HR (1) HRP20150077T1 (pt)
IL (1) IL177488A0 (pt)
ME (1) ME02058B (pt)
NZ (1) NZ549266A (pt)
PL (1) PL1733037T3 (pt)
PT (1) PT1733037E (pt)
RS (1) RS53774B1 (pt)
RU (1) RU2433185C2 (pt)
SI (1) SI1733037T1 (pt)
WO (1) WO2005087802A2 (pt)
ZA (1) ZA200606913B (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0507233B8 (pt) * 2004-03-11 2021-05-25 Genentech Inc processo para a produção de um polipeptídeo heterólogo em e. coli
US8340883B2 (en) * 2005-09-15 2012-12-25 Continental Teves Ag & Co. Ohg Method and apparatus for predicting a movement trajectory
JP2009095237A (ja) 2006-02-02 2009-05-07 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
SG11201404991YA (en) 2012-02-23 2014-09-26 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
JP6787894B2 (ja) 2014-12-05 2020-11-18 アレクシオン ファーマシューティカルズ， インコーポレイテッド 組換えアルカリホスファターゼを用いた発作の処置
CA2973883A1 (en) 2015-01-28 2016-08-04 Alexion Pharmaceuticals, Inc. Methods of treating a subject with an alkaline phosphatase deficiency
JP6993961B2 (ja) * 2015-08-17 2022-01-14 アレクシオン ファーマシューティカルズ， インコーポレイテッド アルカリホスファターゼの製造
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
RU2761555C2 (ru) 2015-10-22 2021-12-09 Джуно Терапьютикс Гмбх Способы, наборы, средства и устройства для трансдукции
EP3368062A4 (en) 2015-10-30 2019-07-03 Alexion Pharmaceuticals, Inc. METHODS OF TREATING CRANIOSYNOSTOSIS IN A PATIENT
KR20180125979A (ko) 2016-04-01 2018-11-26 알렉시온 파마슈티칼스, 인코포레이티드 알칼리성 포스파타아제로 근육 약화의 치료
KR20190129058A (ko) 2017-03-31 2019-11-19 알렉시온 파마슈티칼스, 인코포레이티드 성인 및 청소년에서 저포스파타제증 (hpp)을 치료하는 방법
AU2018260380B2 (en) 2017-04-27 2025-02-13 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
WO2019190752A1 (en) 2018-03-30 2019-10-03 Alexion Pharmaceuticals, Inc. Manufacturing of glycoproteins
WO2020033867A2 (en) 2018-08-10 2020-02-13 Alexion Pharmaceuticals, Inc. Methods of treating neurofibromatosis type 1 and related conditions with alkaline phosphatase
WO2020089343A1 (en) 2018-10-31 2020-05-07 Juno Therapeutics Gmbh Methods for selection and stimulation of cells and apparatus for same
CA3161266A1 (en) 2019-12-09 2021-06-17 Alexion Pharmaceuticals, Inc. Alkaline phosphatase polypeptides and methods of use thereof
US12611447B2 (en) 2020-09-04 2026-04-28 Alexion Pharmaceuticals, Inc. Methods for treating bone mineralization disorders
US12426594B2 (en) 2020-09-24 2025-09-30 Everest Medical Innovation GmbH Cryoprotective compositions and methods for protection of a surgical site during cryosurgery
US12453805B2 (en) 2020-09-24 2025-10-28 Everest Medical Innovation GmbH Cryoprotective compositions, surgical kits, and methods for protection of a surgical site during cryosurgery
AU2022218782B2 (en) 2021-02-12 2026-06-25 Alexion Pharmaceuticals, Inc. Alkaline phosphatase polypeptides and methods of use thereof
CN113308393A (zh) * 2021-04-20 2021-08-27 上田环境修复有限公司 一种降解氨氮和总氮的制剂及其制备方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304472A (en) * 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
CN1266275C (zh) * 1995-06-07 2006-07-26 丹尼斯科公司 重组己糖氧化酶和其产生方法及用途
CA2225378C (en) 1995-06-29 2012-04-17 Immunex Corporation Cytokine that induces apoptosis
US6030945A (en) 1996-01-09 2000-02-29 Genentech, Inc. Apo-2 ligand
EP1001990A4 (en) 1997-06-26 2001-12-19 Immunex Corp PROTEIN BINDING TO TRAIL PROTEIN
RU2139343C1 (ru) * 1997-10-31 1999-10-10 Государственный научный центр прикладной микробиологии Питательная среда для выделения возбудителя геморрагического колита с гемолитико-уремическим синдромом, вызываемого кишечной палочкой 0157:н7 (сорбитол e.coli 0157:н7агар)
WO2000055353A1 (en) * 1999-03-17 2000-09-21 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
US6337191B1 (en) * 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
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RU2165455C1 (ru) * 1999-11-23 2001-04-20 Государственный научно-исследовательский институт особо чистых биопрепаратов РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pSS5, КОДИРУЮЩАЯ СИНТЕЗ РЕКОМБИНАНТНОГО ЧЕЛОВЕЧЕСКОГО АЛЬФА-2b ИНТЕРФЕРОНА, ШТАММ ESCHERICHIA COLI SS5 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ЧЕЛОВЕЧЕСКОГО АЛЬФА-2b ИНТЕРФЕРОНА И СПОСОБ ПОЛУЧЕНИЯ ИНТЕРФЕРОНА АЛЬФА-2b
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BRPI0507233B8 (pt) * 2004-03-11 2021-05-25 Genentech Inc processo para a produção de um polipeptídeo heterólogo em e. coli

Also Published As

Publication number Publication date
CA2558911C (en) 2016-12-06
KR20070029144A (ko) 2007-03-13
KR101210083B1 (ko) 2012-12-07
AU2005222397A1 (en) 2005-09-22
CA2558911A1 (en) 2005-09-22
HK1103761A1 (en) 2007-12-28
EP1733037A2 (en) 2006-12-20
DK1733037T3 (en) 2015-01-12
IL177488A0 (en) 2006-12-10
BRPI0507233B1 (pt) 2018-01-23
ZA200606913B (en) 2008-03-26
PL1733037T3 (pl) 2015-04-30
WO2005087802A3 (en) 2006-03-02
US7294483B2 (en) 2007-11-13
ME02058B (me) 2015-05-20
US20110059484A1 (en) 2011-03-10
WO2005087802A2 (en) 2005-09-22
RS53774B1 (sr) 2015-06-30
HRP20150077T1 (hr) 2015-04-10
RU2433185C2 (ru) 2011-11-10
SI1733037T1 (sl) 2015-02-27
CN1954074A (zh) 2007-04-25
ES2527291T3 (es) 2015-01-22
US20070026496A1 (en) 2007-02-01
US20050244927A1 (en) 2005-11-03
JP5014116B2 (ja) 2012-08-29
US20130236930A1 (en) 2013-09-12
BRPI0507233A (pt) 2007-06-26
JP2007537725A (ja) 2007-12-27
NZ549266A (en) 2008-07-31
EP1733037B1 (en) 2014-11-05
PT1733037E (pt) 2015-02-05
CA2946323A1 (en) 2005-09-22
RU2006135933A (ru) 2008-04-20
CN1954074B (zh) 2011-08-24
US8389242B2 (en) 2013-03-05

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
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